Professional Documents
Culture Documents
Biomedical Instrumentation
for
Instrumentation Engineering
By
www.thegateacademy.com
Syllabus
A.O.B
Percentage of marks
2013
3.0
2012
6.0
2011
2.0
2010
9.0
2009
11.0
2008
16.0
2007
16.0
2006
14.66
2005
12.66
2004
25.0
2003
18.0
Overall Percentage
12.12%
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Contents
A.O.B
CONTENTS
Chapter
#1.
#2.
#3.
#4.
#5.
Page No.
1 - 15
1-3
3-7
7-9
10 - 11
11 - 12
13
13 - 15
Analytical Instrumentation
Beer Lamberts law
Infrared Spectroscopy Instrumentation
Assigment 1
Assigment 2
Answer Keys
Explanations
Mass Spectrometer
16 - 22
16 - 17
17 - 18
19 - 20
21
21 - 22
Introduction
Time of Flight Mass Spectrometer
Assignment
Answer Keys
Explanations
23 - 34
23 - 24
24 - 26
26 - 28
29 - 30
30 - 31
32
32 - 34
Origin of X rays
X-ray Diffraction Braggs Law
Nuclear Detectors
Assignment 1
Assignment 2
Answer Keys
Explanations
35 - 55
35 - 37
37 - 41
41 - 49
50 - 51
51 - 52
53
53 - 55
Optical Sources
LASER
Photo Detectors
Assignment 1
Assignment 2
Answer Keys
Explanations
Introduction
Michelsons Interferometer Working
Application in Metrology
Assignment
56 63
56
56 - 57
57 - 58
59 - 60
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Contents
Answer Keys
Explanations
#7.
#8.
#9.
Introduction
Construction
Fibre Characteristics and Classification
Assignment 1
Assignment 2
Answer Keys
Explanations
A.O.B
61
61 - 63
64 76
64
64 - 66
66 - 69
70 - 71
71 - 72
73
73 - 76
77 - 83
77
77
78 - 79
79
80 - 81
82
82 - 83
Introduction
Acoustic Impedence(z)
Ultrasonic Transducers
Doppler Shift Ultrasound Transducer
Assignment
Answer Keys
Explanations
84 - 102
84 - 87
87 - 89
89 - 91
91 - 94
95 - 96
97 - 98
99
99 - 102
Introduction
Measurement of Blood Pressure
Measurement of Blood Volume
Measurement of Heart Sounds
Test on Blood Cells
Principle of Computer Assisted Tomography
Assignment
Answer Keys
Explanations
103 - 114
103
103 - 104
104
105
105 - 109
109 - 110
111 - 112
113
113 - 114
THE GATE ACADEMY PVT.LTD. H.O.: #74, Keshava Krupa (third Floor), 30th Cross, 10th Main, Jayanagar 4th Block, Bangalore-11
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Contents
A.O.B
Module Test
115 - 126
Test Questions
Answer Keys
Explanations
115 - 119
120
120 - 126
Reference Books
127
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Chapter 1
A.O.B
CHAPTER 1
U.V, Visible and IR spectrometry
Analytical Instrumentation
Analytical instruments are primarily used to obtained qualitative and quantitative
information regarding the composition of a given unknown sample.
The basic building blocks are:
Chemical
information
source
Analytical
instrument
Signal
conditioner
Display
system
ergs-s (or)
Joules-s
: frequency
If is the wavelength interval between successive maxima and minima of the wave), then
C =
Where C: velocity of propagation of radiant energy in vacuum.
Interaction of radiation with matter
S. No
Radiation absorbed
Energy changes involved
1.
Visible, ultraviolet, x Electronic transitions, vibrational
ray
rotational changes
or
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Page 1
Chapter 1
2.
Infrared
3.
4.
Microwave
Radio frequency
A.O.B
300 m
10 m
0.67 m
30 m
3 cm
7000
3000
30 3
7000 4000
MICROWAVES
FREQUENCY RANGE
OF HUMAN EYE
EXTRA HIGH
VERY LOW
MEDIUM
HIGH
VERY HIGH ULTRA HIGH SUPER HIGH
LOW
FREQUENCY INFRARED
FREQUENCY FREQUENCY FREQUENCY FREQUENCY FREQUENCY FREQUENCY FREQUENCY
10 kHz
100 kHz
1 MHz
30 MHz
450 MHz
NUCLEAR QUADRUPOLE
RESONANCE 2 1000 MHz
1 GHz
10 GHz
ELECTRON SPIN
RESONANCE; X-BAND
9.46 GHz
300 GHz
4.3
VISIBLE
ULTRAVIOLET
X-RAY
INFRARED
SPECTROSCOPY 1 MM2.5 M 10 4000 cm
RAMAN SPECTROSCOPY
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Page 2
Chapter 1
A.O.B
Absorbed
Radiation
Transmitted Radiation
Incident
Radiation
Sample
Absorption spectroscopy is based on the principle that the amount of absorption that occurs
is dependent on the number of molecules present in the sample.
Here the analysis is done by studying the intensity of the radiant power leaving the
substance, i.e., the transmitted radiation which is an indication of concentration of the
sample.
The absorbance is calculated as;
Transmittance (T)
where:
p: energy transmitted
P : Incident energy
Absorbance
log ( )
log
Optical density
( )
log (
Chapter 1
A.O.B
abc
log ( ) and T =
Assumptions
1.
2.
3.
The instrument module for UV and visible spectrometry can be pictorized as below
Example: The transmittance of a coloured solution is 0.5, the absorption of the solution is?
A = log
= log
= 0.3
Example: In a particular sample the absorption is 0.6 for a molar concentration of the solute of
1.0
moles and 2cm path length the molar absorptivity is?
A = abc
a=
Substitute a = 3000
Radiant
Source
Wavelength
Selector
Solvent
Photo
detector
Read out
device
Sample
Chapter 1
A.O.B
952cm-1 and d =
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