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n , =
!
, ''
i
''
i
, H
.
/ . '
i
"
#he reverse reactions are:
. '
i
''
i
, H
.
/
''
i
, polynucleotide
n , =!
polynucleotide
n!
, 9#',
9ot:
polynucleotide
n , =!
, H
.
/ polynucleotide
n!
, 9I' nucleoside
monophosphate!,
which is the reaction by which $9) is hydroly;ed" a! and b! are untrue:
en;ymes cataly;e both forward and reverse reactions, and hydrolysis is an
energetically favorable reaction" d! is untrue, since en;ymes are unchanged by
participating in catalysis" Whether e! is true or not for any particular reaction is
irrelevant"
3-38 In order to add the * nucleotide to the polynucleotide chain, it must be in the form of a
*#' cytidine triphosphate!" *onversion of *I' to *#' occurs by the sequential transfer
of two terminal phosphate groups from two molecules of )#'" #hus, hydroylysis of )#'
is coupled to phosphorylation of *I' and then to phosphorylation of *3'" +ubsequently,
the reaction that adds *I' to the polynucleotide chain releases pyrophosphate ''i!,
which is hydroly;ed to inorganic phosphate: this favorable reaction provides the energetic
drive for the overall condensation or polymeri;ation! reaction"
+*