Mek8222 TRM B

Automated
Hematology Analyzer
MEK-8222J/K
0634-002048B
If you have any comments or suggestions
on this manual, please contact us at:
www.nihonkohden.com
Copyright Notice
The entire contents of this manual are copyrighted by Nihon Kohden. All rights are reserved. No part of this
document may be reproduced, stored, or transmitted in any form or by any means (electronic, mechanical,
photocopied, recorded, or otherwise) without the prior written permission of Nihon Kohden.
N.1 Technical Reference Manual MEK-8222
After signing this non-disclosure agreement or equivalent, you can use the technical reference manual.

Non-Disclosure Agreement (Sample)

THIS AGREEMENT between __________________________ (COMPANY) and Nihon Kohden Corporation (NK)
effective from the date stated below by COMPANY, shall govern the conditions of disclosure of the Technical
Reference Manual and other confidential technical information given to COMPANY by NK relating to Nihon
Kohden hematology analyzer ____________________(collectively "Confidential Information").

COMPANY hereby agrees not to disclose Confidential Information to any other party or copy the Confidential
Information without the express written consent of NK. Upon request of NK, COMPANY shall immediately return
the Technical Reference Manual, including all copies, to NK.

THIS AGREEMENT shall not apply to the following information.
(a) Publicly known before or after disclosure by NK through no fault of COMPANY.
(b) In the possession of COMPANY before disclosure by NK.
(c) Acquired by COMPANY from a third party having an independent right to disclose the information.

If COMPANY causes damage to NK against THIS AGREEMENT, COMPANY shall make compensation for the
damage.

When any discrepancy occurs in the interpretation of THIS AGREEMENT or when any discrepancy occurs between
COMPANY and NK in regard to matters not stipulated in THIS AGREEMENT, COMPANY and NK shall discuss
the discrepancy amicably in order to reach a solution in good faith.

Two copies of THIS AGREEMENT shall be made and signed by COMPANY and NK. COMPANY and NK shall
each have one copy of THIS AGREEMENT.

COMPANY NK
Date: ______________________________________ Date: _____________________________________
Company: __________________________________ Nihon Kohden Corporation
Address: ___________________________________ 1-31-4, Nishiochiai, Shinjuku-ku, Tokyo
___________________________________________

Signature: __________________________________ Signature: _________________________________
Signee's name in block letters:

___________________________________________
CONTENTS
Technical Reference Manual MEK-8222 C.1
Contents
Section 1 General .................................................................................. 1C.1
Connection Cable List .......................................................................................................... 1.1
Connection and Wiring Diagram............................................................................................ 1.3
Hydraulic System Diagram................................................................................................... 1.4
Tube List ............................................................................................................................... 1.5
Section 2 Replaceable Parts List......................................................... 2C.1
Units and Boards.................................................................................................................. 2.2
Chassis ................................................................................................................................ 2.4
MO-820V Laser Optical Unit ................................................................................................. 2.6
MF-820V Flowcell Unit ......................................................................................................... 2.7
JQ-820V Inlet/Outlet Unit ..................................................................................................... 2.8
JQ-821V Left Side Piping Unit .............................................................................................. 2.9
JQ-822V Center Piping Unit ............................................................................................... 2.10
JQ-823V 4-channel Liquid Sensor Unit ............................................................................... 2.11
PV-820V Display Unit ......................................................................................................... 2.12
ZY-820V Warmer Piping Unit ............................................................................................... 2.13
ZK-821V/VG CCD Bar Code Reader Unit (Option) .............................................................. 2.14
MB-820V Sub Bath Unit ..................................................................................................... 2.15
MC-820V CBC Measuring Unit ........................................................................................... 2.16
MC-821V Flow Cytometry Piping Unit ................................................................................ 2.18
MP-520V Pump Unit ........................................................................................................... 2.20
MP-821V Sample Pump Unit .............................................................................................. 2.21
MP-820V Complex Pump Unit ............................................................................................ 2.22
MP-822V Sheath Pump Unit ............................................................................................... 2.24
MS-820V Closed Sampler Unit ........................................................................................... 2.26
MS-821V Open Sampler Unit ............................................................................................. 2.28
MS-822V Table Unit ............................................................................................................ 2.29
PV-820V Display Unit ......................................................................................................... 2.30
ZY-820V Warmer Piping Unit ............................................................................................... 2.31
ZK-821V/VG CCD Bar Code Reader Unit (Option) .............................................................. 2.32
CONTENTS
C.2 Technical Reference Manual MEK-8222
This page is intentionally left blank.
Technical Reference Manual MEK-8222 i
EMC RELATED CAUTION
This equipment and/or system complies with the International Standard EN61326-1 for electromagnetic
compatibility for medical electrical equipment and/or system. However, an electromagnetic environment that
exceeds the limits or levels stipulated in the EN61326-1, can cause harmful interference to the equipment
and/or system or cause the equipment and/or system to fail to perform its intended function or degrade its
intended performance. Therefore, during the operation of the equipment and/or system, if there is any
undesired deviation from its intended operational performance, you must avoid, identify and resolve the
adverse electromagnetic effect before continuing to use the equipment and/or system.
The following describes some common interference sources and remedial actions:
1. Strong electromagnetic interference from a nearby emitter source such as an authorized radio station or
cellular phone:
Install the equipment and/or system at another location if it is interfered with by an emitter source such
as an authorized radio station. Keep the emitter source such as cellular phone away from the equipment
and/or system.
2. Radio-frequency interference from other equipment through the AC power supply of the equipment and/
or system:
Identify the cause of this interference and if possible remove this interference source. If this is not
possible, use a different power supply.
3. Effect of direct or indirect electrostatic discharge:
Make sure all users and patients in contact with the equipment and/or system are free from direct or
indirect electrostatic energy before using it. A humid room can help lessen this problem.
4. Electromagnetic interference with any radio wave receiver such as radio or television:
If the equipment and/or system interferes with any radio wave receiver, locate the equipment and/or
system as far as possible from the radio wave receiver.
If the above suggested remedial actions do not solve the problem, consult your Nihon Kohden Corporation
subsidiary or distributor for additional suggestions.
The CE mark is a protected conformity mark of the European Community. The products herewith comply
with the requirements of the IVD Directive 98/79/EEC.
This equipment complies with International Standard EN55011 (1999) Group 1, Class B. Class B
EQUIPMENT is equipment suitable for use in domestic establishments and in establishments directly
connected to a low voltage power supply network which supplies buildings used for domestic purposes.
ii Technical Reference Manual MEK-8222
Technical Reference Manual MEK-8222 iii
Conventions Used in this Manual and Instrument
Warnings, Cautions and Notes
Warnings, cautions and notes are used in this manual to alert or signal the reader to specific information.
WARNING
A warning alerts the user to the possible injury or death associated with the use or misuse of the
instrument.
CAUTION
A caution alerts the user to possible injury or problems with the instrument associated with its use or
misuse such as instrument malfunction, instrument failure, damage to the instrument, or damage to other
property.
NOTE
A note provides specific information, in the form of recommendations, prerequirements, alternative methods
or supplemental information.
iv Technical Reference Manual MEK-8222
Symbol Description Symbol Description
Main power lamp
HEMOLYNAC3
(hemolysing reagent)
On only for part of the equipment
HEMOLYNAC5
(hemolysing reagent)
Off only for part of the equipment WASTE
Laser ON
Hand positions for carrying the
instrument
Reset RS-232C socket
Manual USB socket
Clean IVD conformity
Eject Fuse
Start
AC power off
(Disconnection from the mains)
Emergency
AC power on
(Connection to the mains)

Do not touch the sample rack
during measurement
Alternating current
Inlet Equipotential terminal
Outlet Year of manufacture
Attention, consult operator's manual Serial number

ISOTONAC3
(diluent)

CLEANAC
(detergent)

CLEANAC3
(detergent)

The CE mark is a protected
conformity mark of the European
Community. The products herewith
comply with the requirements of the
IVD Directive 98/79/EEC.

Explanations of the Symbols in this Manual and Instrument
The following symbols found in this manual/instrument bear the respective descriptions as given.
On panel
IVD
Technical Reference Manual MEK-8222 v
On screen and recorded data
Symbol Description Symbol Description

Beside WBC or RBC measured
value: Sample error
Beside HGB measured value: Dirty
measurement baths

Beside HGB measured value: HGB
circuit error

Beside WBC measured value: Poor
hemolyzation
Beside HGB measured value: HGB
voltage adjustment error

Beside WBC or PLT measured
value: Platelet coagulation

C
Technical Reference Manual MEK-8222 1C.1
Section 1 General
Connection Cable List ......................................................................................................... 1.1
Connection and Wiring Diagram........................................................................................... 1.3
Hydraulic System Diagram.................................................................................................. 1.4
Tube List .............................................................................................................................. 1.5
1. GENERAL
Technical Reference Manual MEK-8222 1.1
Connection Cable List
Cable No. Unit NK code Cable model or description Cable ends
CNP 306 JQ-820V 261753 HKP-6F02-3BT 4WAY LIQUID SENSOR board 2 valves
CNP 238 JQ-821V 374391 MFC20HF DRIVER2 board 7 valves
CNA 205 JQ-822V 622489B
PHR-4/HKP-8F02-4BT
(W170)
DRIVER1 board LIQUID LEVEL SENSOR board
CNA 206 JQ-823V 620393C 1-171822-2 (W300) DRIVER1 board 4WAY LIQUID SENSOR board
CNA 220 620384 PHDR-12VS/ZHR6 (W280) DRIVER4 board 2 motors
CNA 221
MB-820V
620366
PHDR-10VS/171822-3
(W280)
DRIVER4 board 2 sensors
CNA 239 620321A
171822-3/1-0480435-0
W390
DRIVER2 board Manual count switch
CNP 240 374382 MFC-16HF DRIVER2 board 7 valves
CNA 241 620339A 171822-3/(W70) PRE AMP board Electrode for RBC
CNA 242 620339A 171822-3/(W70) PRE AMP board Electrode for WBC
CNA 243 620348A 171822-6/M63M83-07 (W70) PRE AMP board Phototransistor of RBC manometer
CNA 244 536582 M63M83-04 (S80) PRE AMP board LED of RBC mamometer
CNA 245 620348A 171822-6/M63M83-07 (W70) PRE AMP board
Phototransistor of WBC
manometer
CNA 246 536582 M63M83-04 (S80) PRE AMP board LED of WBC mamometer
CNA 247
MC-820V
620357A
M63M83-08/M63M83-04
(W140)
PRE AMP board HGB AMP and HGB LED boards
CNP 204 MC-821V 374382 MFC16HF DRIVER1 board Valve
CNA 103 MO-820V 529652A
MFC16/M63M83/171822-4
(W690)
MASTER board
MO preamp board set and MO LD
DRIVER board
CNA 240 MP-520V DRIVER1 board 5-part WBC pump unit
CNA 236 MP-520V DRIVER1 board RBC pump unit
CNA 237 MP-520V DRIVER1 board WBC pump unit
CNP 222 374391 MFC-20HF DRIVER4 board 2 motors and 4 valves
CNA 223 620099C 1-171822-0 (W580) DRIVER4 board MD CONNECTION board
CNA 224
MP-820V
620081C 171822-4 (W300) DRIVER4 board MP CONNECTION board
CNP 201 374408 MFC10HF DRIVER1 board 2 valves and motor
CNA 202
MP-821V
620107B 171822-8/171822-3 (W330) DRIVER1 board 2 sensors
CNA 105 619011A PHDR-28VS (W230) MASTER board SHEATH CONTROL board
CNP 207 374382 MFC16HF SHEATH CONTROL board 5 valves and motor
CNA 208
MP-822V
619002 PHR-4 (W50) SHEATH CONTROL board LIQUID LEVEL SENSOR board
CNA 014 619992 VHR-6N (W370) SLAVE MS board DRIVER3 board
CNA 109 620009 HIF3BA-50D-AC-27 SLAVE MS board DRIVER3 board
CNA 227 620018C MFC26HF/PHR-11/(W260) DRIVER3 board 3 motors and 3 valves
CNP 227 374408 MFC10HF DRIVER3 board 2 motors
CNA 228 620036B
PHDR-20VS/171822-6
(W400)
DRIVER3 board
3 sensors and 3POS SENSOR
board
CNA 229
MS-820V
620045A
PHDR-24VS/171822-3
(W350)
DRIVER3 board 6 sensors
CNP 226 374382 MFC16HF DRIVER4 board 2 motors and valve
CNA 225
MS-821V
620054B
PHDR-18VS/171822-3
(W290)
DRIVER4 board 3 sensors and SENSOR board
CNA 015 620125A VHR-6N (W500) SLAVE MS board DRIVER5 board
CNA 110 620116A HIF3BA-60D-AA-47 SLAVE MS board DRIVER5 board
CNA 230 536591 171822-8/PHR-11 (W85) DRIVER5 board Motor
CNP 231 149287 171822-7 DRIVER5 board Motor
CNA 232 620134A 171822-6 (W260) DRIVER5 board SENSOR3 board
CNA 233
MS-822V
620143A HIF3BA-16D-CC-27 DRIVER5 board TABLE SENSOR board

1. GENERAL
1.2 Technical Reference Manual MEK-8222
Cable No. Unit NK code Cable model or description Cable ends
CNA 209 601716
Dedicated cable for touch
screen
LCD CONTROL board Touch screen
CNA 210 622444 PHDR-16VS (W430) LCD CONTROL board MANUAL KEY board
CNA 211 622453A PHDR-12VS (W330) LCD CONTROL board RACK KEY board
CNA 212 622462 DF19G-30S-1C (W40) LCD CONTROL board LCD
CNA 213 601707 Dedicated cable for LCD LCD CONTROL board LCD
CNA 214 575726A
51021-0800/PHDR-10VS
(W100)
LCD CONTROL board Inverter board
CNA 215 622471A PHR-7/XHP-7 (W65) LCD CONTROL board Inverter board

PV-820V
539463 NA19002-4220 (FLCV-08) Inverter board
CNA 113 620268A
PHDR-20VS/MFC-20HF
(W135)
MASTER board INTERFACE board
CNA 216 622515A
PHDR-20VS/JEC-9P/-25S
(W60)
INTERFACE board Rear panel
CNA 301 620277A PHDR-10VS (W60) MASTER board SLAVE MS board
CNA 302 620286 PHDR-10VS (W430) MASTER board SLAVE CBC board
CNA 303 620277A PHDR-10VS (W60) SLAVE MS board SLAVE CBC board
CNA 304 622524A 171822-4 (S130) 4WAY LIQUID SENSOR board Emergency switch
374426 LP2S-17S-889 Emergency switch
CNA 305 622533A 171822-2 (S100) 4WAY LIQUID SENSOR board Laser key switch
374417 SK-12BAS1 Laser key switch
CNA 008 536617 VHR-6N (W200) MASTER board DRIVER1 board
CNA 102 620446A HIF3BA-40D-AC-17 MASTER board DRIVER1 board
CNA 010 620401 VHR-6N (W290) SLAVE CBC board DRIVER2 board
CNA 011 620428 VHR-2N (W200) SLAVE CBC board DRIVER2 board
CNA 106 620455 HIF3BA-50D-AC-17 SLAVE CBC board DRIVER2 board
CNA 013 620125A VHR-6N (W500) SLAVE MS board DRIVER4 board
CNA 108 620464B HIF3BA-64D-AC-57 SLAVE MS board DRIVER4 board
CNA 009 622426A VHR-5N (W1050) MASTER board LCD CONTROL board
CNA 104 622435B HIF3BA-60-AC-117 MASTER board LCD CONTROL board
CNA 012 620303A VHR-8N (W400) SLAVE CBC board PRE AMP board
CNA 107
RK-820V
620312B HIF3BA-40D-AA-47 SLAVE CBC board PRE AMP board
CNA 001 419272 41729 L=130
AC inlet socket with main power
switch
Ground termimnal
534486B T-534486A
switch
Power transformer for 100 V AC
534495B T-534495A
switch
534503B T-534503A
switch
539561 L-41-20 POWER board High frequency reduction coil
CNA 002 620197A VHR-10N (W100) POWER board MASTER board
CNA 003 620241 VHR-3N (W360) POWER board MASTER board
CNA 005 620223A VHR-10N (W370) POWER board SLAVE CBC board
CNA 006 620214A VHR-7N (W500) POWER board SLAVE CBC board
CNA 007 620223A VHR-10N (W370) POWER board SLAVE MS board
CNA 101
SC-820V
620232 PHR-6 (W90) POWER board MASTER board
CNA 217 ZK-820V 531489
Dedicated cable for barcode
reader
Rear panel CCD barcode reader
CNA 234 620072 PHDR-12VS/ZHR-6 (w250) DRIVER5 board Motor and sensor
CNP 235 620161 PHDR-10VS DRIVER5 board Bar-code reader

ZK-821V
531578 BL-185 DRIVER5 board Bar-code reader
CNA 004 622498 VHR-2N (W600) POWER board WARMER CONTROL board
CNA 112 622506A PHDR-12VS (W660) MASTER board WARMER CONTROL board
CNA 218 536983
Dedicated cable for container
heater
WARMER CONTROL board Container heater
CNP 219
ZY-820V
374408 MFC10HF DRIVER4 board 2 valves
CNA 241 278111
Dedicated cable for card
printer
Rear panel Card printer
CNA 242 584351A
DSUB-HAB13-FC07
(L=0.7m)
Rear panel Personal computer
CNA 243 514026A
DSUB-HAB13-FC20
(L=2.0m)
Rear panel Personal computer

1. GENERAL
Connection and Wiring Diagram
Display unit PV-820V
Inverter board
POWER board UT-7160
DRIVER5 board
UT-7156
Left side piping unit
JQ-821V
Connection and Wiring Diagram
H8S
CPU
CCD barcode
reader ZK-822V
(Option)
Power supply unit SC-820VK
Sheath pump unit MP-822V
SHEATH CONTROL board
UT-7158
Flow cytometry piping unit
MC-821V 2MV
1 2MV
2
PMV
7
2MV
3
PMV
5
2MV
4
3MV
8
Laser optical unit MO-820V
MO preamp board set UT-7172
LD DRIVER board UT-7176
Touch screen
MANUAL KEY board
INTERFACE board UT-7161
3MV
1
3MV
2
3MV
1
3MV
4
3MV
5
3MV
6
3MV
7
WARMER CONTROL board
UT-7159
CBC measuring unit MC-820V
MANOMETER
PTR board
2MV
1
2MV
2
2MV
3
2MV
4
3MV
5
2MV
6
3MV
7
Pump unit MP-520V (5-part WBC)
MT
PH
Position Sensor
Sample pump unit MP-821V
Open sampler unit MS-821V
3MV
1
3MV
2
Table unit
MS-822V
4WAY LIQUID SENSOR board
DRIVER1 board
UT-7152
3MV
2
3MV
3
Complex pump unit
MP-820V
DRIVER2 board
UT-7153
MT
PH
MT
PH
MT
PX
3MV
1
3MV
2
3MV
3
MT
PX
3MV
4
3MV
1
DRIVER3 board
UT-7154
3MV
1
3MV
2
3MV
3
TABLE SENSOR board UT-7157
P
i
RACK KEY board
Switch LED
Eject
Start
Emergency
Clean
USB
LCD CONTROL board
MTR
SPG
MTW
SPG
LIQUID LEVEL
SENSOR board
UT-7171
LIQUID LEVEL SENSOR board
UT-7171
Laser switch Emergency stop switch
MD CONNECTION board
MD SET
SW1
MT1
PX
MT2
PX
MT1
PX
MT2
DC
MT1
PK5
MT2
PX
MT3
PK5
MT1
PK5
MT2
PX
MT
SPG
2MV
2MV
1
PK
243
RS-232C
DSUB 9P
RS-232C
DSUB 25P
Data Management
Program for PC
Qi-820V (Option)
Handy Barcode
Reader Unit
ZY-820V (Option)
Impact Printer WA-711V
(Option) or locally avialable
printer LQ-300, LQ-300+,
LX-300+
Card Printer
WA-460V (Option)
Thermal Printer
WA-820V (Option)
2MV
1
2MV
2
2MV
3
2MV
4
MT1
DC
2MV
5
50P
LED board
HGB
AMP
board
HGB
LED
Pump unit MP-520V (RBC)
MFC20P
MFC16P
MFC10P
VH6P
PHD12P
PHD10P
VH10P
VH10P VH10P
VH 7P VH 7P
VH10P
HIF60P
VH6P
VH6P
SENSOR3 board
UT-7086
AMP6P
60P
VH-6P
AMP8P AMP7P
HIF-16P PHD12
BL-185
PHD10
VH6P
PHD20P
PHD24P
UT-7155
2MV
6
POS
CODE0
CODE1
UD1
UD0
UD4
UD5
UD0
UD1
UD2
UD3
CN201
CN203
CN202
HIF50P
CN101
CN001
P
i
P
O
S
0
MFC16P
PHD28P
CN101
CN201
PHD28P
P
i
P
O
S
1
P
i
P
O
S
2
P
i
P
O
S
3
P
i
P
O
S
4
P
i
P
O
S
5
P
i
P
O
S
6
P
i
P
O
S
7
P
i
P
O
S
8
P
i
P
O
S
9
P
i
P
O
S
1
0
P
i
P
O
S
1
1
CN001
VH6P
CN201
CN202
CN203
ORIGIN
ENCODE
MD SET
SW2
RL0,1,2,3
SL0 SL1 AMP10P
UD1
Position sensor UD0
AMP4P
CN205
CN206
CN207
CN208
SENSOR board UT-7065
POS
CODE0
CODE1
UD0
UD1
50P
HIF50P
HIF50P
CN101 CN001
VH6P
HIF-60P
CN101
CN001
CN201 CN202
CN102 CN203
Position sensor POS
CODE0
CODE1
CODE2 CN204 CN205
CN204
UD2
VH5P HIF-60P
VH5P
DC line
AC line
VH6P
Serial communication Iine
VH10P
VH3P
PHK6P
VH10P
VH3P
PHK6P
MFC16P
PHD12P
AMP2P AMP4P AMP12P
MFC10P
MFC16P
AMP8P
AMP9P
UD1
UD0
SEN
PHD12P
SEN
SEN
VH6P
CN002
VH-2P
VH2P 40P
CN001
HIF40P HIF40P
VH8P VH8P
CN001
CN002
CN003
CN101
CN004
CN005
CN102
SLAVE MS board
UT-7148
PHD10P
CN302
PHD10P
CN301
PHD10P
CN301
PHD10P
SLAVE CBC board
UT-7147
PHD10P
CN301
CN302
CN001
HIF50P
CN102
CN003
CN002
64P
HIF64P
HIF-64P
CN101
CN101
CN004
CN103
MFC-26P
PHD10P
CN302
HKP6P
AMP12P
CN201
CN202 CN203
CN101
Up
CN005
CN101
CN003
CN004
CN009
CN007
CN006
CN202
CN201
CN203
CN204
CN205
CN206
VH6P
40P
CN001
HIF40P
CN101
AMP2P
HIF40P
CN001
CN002
CN101
CN104
CN004
CN103
CN003
CN102
CN105
CN303
CN106 CN107
MASTER board
UT-7146
VH2P
CN001 CN101
CN102
PHD12P
CN103
CN301
LCD unit
DF-30P
CN204
SM04P
CN203
SFW15R
PHD16P
PHK7P PHD10P
CN205 CN202
172034-1
CN001
350428-1
CN002
AMP9P
CN203
AMP9P
CN204
CN102
AMP3P
CN103
MFC16P
CN201
MFC20P
CN202
3MV
1
CNA224
CNA223
AMP10P
AMP4P
CNA012
CNA107
CNA211
CNA210
CNA239
AMP3P
CN204
MFC-10P
CNA227
CNP227
CNA009
CNA104
HKP8P
CNA008 CNA102
CNA206
CNA240
CNA219
CNA108 CNA013
CNA
014
CNA
109
VH2P
HIF-60P
CNA216
CNA113
PHD20P
CNA105
CNP207
CNA208
PHKS4P
CN202
CNA305 CNA304
CNA302
CNA301
CNA302
CNA303 CNA303
CNA005
CNA006
CNA007
CNA101
CNA002
CNA003
CNA103
CNA205
CNP238
CNP306
C
N
A
2
0
9
C
N
A
2
1
2
C
N
A
2
1
3
C
N
A
2
1
4
C
N
A
2
1
5
CNA004
CNA112
PHR5
CNA218
CNA228
CNA227
CNA229
CNA220
CNA221
CNP222
CNP225
CNA226
CNA110
CNA015
CNP231 CNA230
CNP235
CNA234
CNA232
CNA233
C
N
A
0
1
0
C
N
A
0
1
1 CNA106
CNA237
CNA236
CNA202
CNP201
C
N
A
2
4
2
C
N
A
2
4
3
CNA241 CNA217
CNP240
CNP238
CNA247
WBC RBC
CNA243
CNA246
C
N
A
2
4
2
PTR board
CNA242
CNA245
C
N
A
2
4
1
PHD18P
Eject
Start
Emergency
Clean
LED Switch
Main power
Power
Laser
Operation indicator
Manual mode
Power key
Manual mode key
Reset key
H8S
CPU
4-Channel liquid sensor unit JQ-823V
UT-7179
Depressure needle
Upper Position
Position sensor
Lower Position
Position sensor
Pump unit MP-520V (WBC)
Manual
count
switch
PRE AMP board UT-7143
Spare
Turn Up and Down
Upper position
Lower position
Middle position
Down Position sensor
Position sensor
Position sensor
Position sensor
Position sensor
Position sensor
Position Sensor
Position Sensor
Position Sensor
3POS SENSOR board UT-7185
Closed sampler unit MS-820V
Warmer unit ZY-820V
Container
with
heater
for MP
for MD (Diluter)
Up and Down Turn
Turn Up and Down
(Needle)
Up and Down
(Nozzle)
Right and Left Back and Forth
Upper position
Upper position
Lower position
Lower position
Manual sampling nozzle
DRIVER4 board
H8S
CPU
Center piping unit
JQ-822V
Inlet/outlet unit
JQ-820V
RBC WBC
LED board
LED board
LED board
board
A
p
e
r
tu
r
e
c
a
p
A
p
e
r
tu
r
e
c
a
p
WBC
RBC
Sub bath unit MB-820V
Position sensor
Position sensor
Position sensor
Depressure needle Middle position
Upper position
Middle position
Lower position
Sample tube
For mixing
Sampling nozzle
Sampling nozzle
Sampling nozzle
Depressure needle
2
MANOMETER
MP CONNECTION board
1. GENERAL
Hydraulic System Diagram
1. GENERAL
<
M
P
-
8
2
0
V
>
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9
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4
Y
K
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5
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Y
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3
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1
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1
1
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2
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1
3
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2
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1
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3
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3
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2
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3
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6
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4
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1
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M
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(
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1
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m

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Tube List
N
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7
.
5

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4
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1
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2
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2
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1
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1
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4
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5
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1
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1
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2
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2
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4
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2
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M
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R
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m
a
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k
s

1

Y
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0
8
5

2

Y
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0
8
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3

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0
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4

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1
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5

Y
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4
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6

Y
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1
4
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7

Y
K
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5

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Y
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1
2
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Y
K
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Y
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2
3
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1

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1
2

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1
3

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1
4

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1
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1
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3
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1
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0
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1
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=
5
5

1
9

Y
Z
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2
8
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P
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v
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e

t
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2

e
a
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=
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0

1. GENERAL
<
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2
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N
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(
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Q
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3
)
Technical Reference Manual MEK-8222 2C.1
Section 2 Replaceable Parts List
Units and Boards................................................................................................................. 2.2
Chassis ............................................................................................................................... 2.4
JQ-820V Inlet/Outlet Unit .................................................................................................... 2.6
JQ-821V Left Side Piping Unit ............................................................................................. 2.7
JQ-822V Center Piping Unit ................................................................................................ 2.8
JQ-823V 4-channel Liquid Sensor Unit ................................................................................ 2.9
MB-820V Sub Bath Unit .................................................................................................... 2.10
MC-820V CBC Measuring Unit .......................................................................................... 2.12
MC-821V Flow Cytometry Piping Unit ............................................................................... 2.14
MF-820V Flow Cell Unit ..................................................................................................... 2.16
MO-820V Laser Optical Unit .............................................................................................. 2.17
MP-520V Pump Unit .......................................................................................................... 2.18
MP-820V Complex Pump Unit ........................................................................................... 2.20
MP-821V Sample Pump Unit ............................................................................................. 2.22
MP-822V Sheath Pump Unit .............................................................................................. 2.24
MS-820V Closed Sampler Unit .......................................................................................... 2.26
MS-821V Open Sampler Unit ............................................................................................ 2.28
MS-822V Table Unit ........................................................................................................... 2.29
PV-820V Display Unit ........................................................................................................ 2.30
ZY-820V Warmer Piping Unit .............................................................................................. 2.31
ZK-821V/VG CCD Bar Code Reader Unit (Option) ............................................................. 2.32
2. REPLACEABLE PARTS LIST
When ordering parts or accessories from your nearest Nihon Kohden Corporation
distributor, please quote the NK code number and part name which are listed in
this service manual, and the name or model of the unit in which the required part
is located. This will help us to promptly attend to your needs. Always use Nihon
Kohden parts and accessories to assure maximum performance from your
instrument.
Units and Boards
Rank Index NK Code No. Qty Description
D 100 JQ-820V 1 Inlet/outlet unit
D 200 JQ-821V 1 Left side piping unit
D 300 JQ-822V 1 Center piping unit
C 400 JQ-823V 1 4-channel liquid sensor unit
C 500 MB-820V 1 Sub bath unit
C 600 MC-820V 1 CBC measuring unit
D 700 MC-821V 1 Flow cytometry piping unit
C 800 MO-820V 1 Laser optical unit
C 900 MP-520V 3 Pump unit
C 1000 MP-820V 1 Complex pump unit
C 1100 MP-821V 1 Sample pump unit
C 1200 MP-822V 1 Sheath pump unit
C 1300 MS-820V 1 Closed sampler unit
C 1400 MS-821V 1 Open sampler unit
C 1500 MS-822V 1 Table unit
C 1600 PV-820V 1 Display unit
D 1700 RK-820V 1 Chassis
C 1800 MF-820V 1 Flow cell unit
D 1900 ZY-820V 1 Warmer unit
C 2000 SC-820VJ 1 Power supply unit (for J type)
C 2100 SC-820VK 1 Power supply unit (for K type)
C A UT-7146 1 MASTER board
C B UT-7147 1 SLAVE CBC board
C C UT-7148 1 SLAVE MS board
C D UT-7153 1 DRIVER2 board
C E UT-7155 1 DRIVER4 board
C F UT-7160 1 POWER board
C G UT-7161 1 INTERFACE board
Rank
A Requires replacement within about 1 to 3 months.
B Requires replacement within about 6 to 12 months.
C Requires replacement when this component has deterioration or damage.
D Requires replacement which must be done by service personnel trained in Nihon Kohden or by distributor.
1400
600
1000
1300
400
800
1800
900
1500
900
1200
1100
100
700
300
E
D
B
C
A
G
F
Chassis
C 7 532639
PharMed tube 1/16 1/8 (7.5 m)
(Sampling tube)
C 8 6113-042788A 1 Sampling cup
D 9 6111-006457D 1 Top cover
D 10 6113-040923C 1 Right cover
D 11 6113-040861C 1 Left cover
D 12 6111-006484C 1 Front cover
1400
1300
400
900
1500
600
900
8
7
100
1700
Rank
1600
2000
2100
F
C
A
B
G
D
E
12
11
1700
10
9
1700
700
1100
7
1200
500
200
1900
300
1000
1800
800
103
103
2
3
1
4
102
104
104
101
101
105
106
103
103
JQ-820V Inlet/Outlet Unit
C 1 553144 100* O-ring P-3
C 2 6114-095942B 100* Tube fitting nut
C 3 6114-095951A 100* Tube stopper
C 4 XP-513V 19* 3-way valve
D 101 2114-072518C 2 Diluent tube joint (tube joint 6)
D 102 2114-051042A 2 Nut for diluent tube joint
C 103 6114-000591B 4 Detergent/waste tube joint
D 104 6114-000074 4 Nut for detergent/waste tube joint
D 105 6114-067447B 1 Hemolynac 3 tube joint (tube joint 9)
D 106 6114-126134 1 Hemolynac 5 tube joint
* Total quantity per instrument
Rank
C
Requires replacement when this component has deterioration or
damage.
D
Requires replacement which must be done by service personnel trained
in Nihon Kohden or by distributor.
JQ-821V Left Side Piping Unit
Rank
4
4
4
4
4
4
4
JQ-822V Center Piping Unit
C 1 553144 100* O-ring P-3
C 3 6114-095951A 100* Tube stopper
C 301 6113-045321A 1 Liquid chamber
C 302 534316 1 O-ring P-30 (Silicone)
C 303 2114-065847 1 Rubber packing
C 304 2114-080812 1 Stopper
C 305 6113-043154 1 Liquid chamber cap
D H UT-7171 1 LIQUID LEVEL SENSOR board
Rank
H
305
302
301
303
304
1
3
2
4
JQ-823V 4-channel Liquid Sensor Unit
C 401 6113-034476A 4 Bubble sensor
C I UT-7179 1 4WAY LIQUID SENSOR board
Rank
I
401
MB-820V Sub Bath Unit
D 501 553242 2 Stepping motor
C 502 YZ-0283 2 Sub bath 2 assy
Rank
501
502
MC-820V CBC Measuring Unit
Rank
C 1 553144 100* O-ring P-3
C 3 6114-095951A 100* Tube stopper
C 601 6113-043305A 1 Air trap
C 602 6114-126143 1 Air trap cap
C 603 315375A 1 O-ring P-8 (Red silicone)
C 604 6114-095505D 1 Aperture cap (aperture: 100 m in dia.)
C 605 6114-095514D 1 Aperture cap (aperture: 80 m in dia.)
C 606 553198 1 O-ring P-10A (Silicone)
C 607 553233 1 O-ring P-10A (Red Silicone)
C 608 556827 2 O-ring P-10A
C 609 YS-020B6 2 Bath
C 610 6114-036641 1 Hemolysing reagent inlet cap
A 611 T802 2 Hemoglobin filter
B 612 2114-082062B 2 Filter packing S
C 613 YZ-0286 2 Counter assy
C 614 YZ-0287 2 Vacuum chamber (electrode excluded)
C 615 YZ-0288 2 Air chamber
C J UT-7143 1 PRE AMP board
6
1
4
6
1
3
6
1
4
6
1
5
6
1
5
6
0
9
6
1
0
6
1
2
6
1
1
6
0
2
6
0
3
6
0
1
6
0
7
6
0
5
6
0
8
6
0
6
6
0
4
6
0
8
1
3
2
1
3
4
5
J
2
6
1
1
6
1
2
1
3
2
5
5
a
a
6
1
3
MC-821V Flow Cytometry Piping Unit
C 1 553144 100* O-ring P-3
C 3 6114-095951A 100* Tube stopper
C 701 6113-043305A 1 Air trap
C 702 6114-126143 1 Air trap cap
C 703 315375A 1 O-ring P-8 (Red silicone)
C 704 6113-042271 1 Mixing chamber
C 705 6114-120327A 1 Mixing chamber cap
C 707 459264 1 O-ring AS568-004 (Silicone)
D 708 723611 3 Pinch valve TPV-N040
B
709-1
709-2
YZ-0282 2 each
Pinch valve tube set
(709-1: L60, 709-2: L55)
C 710 YZ-0293 1 Silicone tube set (for flow cell unit)
D 711 6114-123048A 1 Tube joint ID05
D 712 6114-122994A 1 Tube joint ID1F
D 713 6114-123003A 4 Tube joint ID1
D 714 2114-051015B 1 Tube ring
D 715 6114-015495B 1 Valve joint A
C K UT-7152 1 DRIVER1 board
Rank
7
0
9
-
1
7
0
9
-
2
7
0
5
7
0
6
7
0
4
7
0
7
5
5
5
7
1
3
4
5
7
1
3
7
0
8
K
7
0
8
7
0
8
7
1
3
7
1
3
7
1
0
7
1
1
231
7
0
2
7
0
3
7
0
1
7
0
9
-
1
7
1
2
1803
1802
1804
1801
801
1805
1806
1806
MF-820V Flow Cell Unit
C 801 532639 PharMed tube 1/16 1/8 (7.5 m)
D 1801 295538 1 O-ring AS568-003
D 1802 6114-124118 1 O-ring 1 4 (Silicone)
D 1803 530597A 1 Flow cell
D 1804 6114-129318 1 Flow cell base
D 1805 565586 2 Steel ball 6
D 1806 358524A 2 O-ring P-6 (Silicone)
Rank
MO-820V Laser Optical Unit
C 710 YZ-0293 1 Silicone tube set (for flow cell unit)
C 801 532639 PharMed tube 1/161/8 (7.5m)
Rank
1800
710
801
MP-520V Pump Unit
D 6 551895 21* Photo-interruptor
A 901 T462 3 Pump tube assy
C 902 YS-003B43 3 Pump rotor assy
Rank
901 902
6
MP-820V Complex Pump Unit
Rank
C 1 553144 100* O-ring P-3
C 3 6114-095951A 100* Tube stopper
C 4-2 XP-503V 3* 3-way valve
C 1001 487296 2 Universal coupling
C 1002 6113-034485B 1 Cylinder block
D 1003 6114-048495B 1 Cylinder bottom cover
D 1004** 6114-108341 1 10 mL piston
D 1005 6144-006261A 1 50 L piston assy
D 1006** 6114-083456B 1 10 mL piston guide
D 1007** 487313 1 Cap seal
C 1008 535903 1 BALSEAL ID20 (X55409B)
C 1009 6114-123396 1 50 L piston guide
C 1010 315348A 1 O-ring P-5 (Silicone)
C 1011 536029 1 BALSEAL ID1.4 (X55408A)
C 1012 6113-041085A 3 Cylinder block
D 1013 6114-125839A 3 Cylinder bottom cover
D 1014 6114-114272B 3 2 mL piston
C 1015 535868 2 BALSEAL ID8 (X203528A)
D 1016 6144-006279B 2 Ball screw assy
C 1017 1 BALSEAL ID8 (-)
D L UT-7177 1 MD/MP connection board set
** 1004, 1006 and 1007 are not replaceable locally. When 1004, 1006 or 1007
replacement is necessary, you must replace the assembly which includes 1004,
1006 and 1007. The assembly model number will be registered in the near
future. Contact Nihon Kohden about the availability.
2
1
3
1012
1015
1008
1002
1010
1009
1011
1001
1001
4
4- 2
4- 2
4- 2
1006
1004
1003
1007
1014
1013
1005
1016
1016
L
L
1017
1101
1102
1104
1
3
2
5
5
6
6
1106 1103
1105
MP-821V Sample Pump Unit
C 1 553144 100* O-ring P-3
C 3 6114-095951A 100* Tube stopper
C 1101 487296 1 Universal coupling
C 1102 6113-043145A 1 Cylinder block
D 1103 6114-114503B 1 Cylinder bottom cover
C 1104 544234 1 BALSEAL ID3 (X200886C)
D 1105 6114-114539B 1 250 L piston
D 1106 6114-114548A 1 Lead screw P2
Rank
MP-822V Sheath Pump Unit
Rank
C 1 553144 100* O-ring P-3
C 3 6114-095951A 100* Tube stopper
C 1201 6113-045321A 1 Liquid chamber
C 1203 2114-065847 2 Rubber packing
C 1204 2114-080812 2 Stopper
C 1205 6113-043154 1 Liquid chamber cap
C 1206 107938 3 O-ring P-6
C 1207 534102 PharMed tube 3/32 7/32 (7.5m)
C 1208 559147 PharMed tube 3 5 (7.5m)
C 1209 541371 1 Check valve
C 1210 548195 1 Air filter
D 1211 534174 1 Compressor
D 1212 548159 10 Hose clip
D 1213 534192 1 Regulator
C 1214 6113-043305A Air trap
C 1215 6114-126143 Air trap cap
C 1216 315375A O-ring P-8 (Red silicone)
C M UT-7158 1 SHEATH CONTROL board
D H UT-7171 1 LIQUID LEVEL SENSOR board
1
2
0
3
1
2
0
4

1
2
0
1

1
2
0
2

1
2
0
5

2

3

1

1
2
0
7

1
2
0
8

1
2
0
9
1
2
0
8

1
2
0
7
1
2
0
6

1

1
2
0
8
1
2
1
0

5

1
2
0
3

1
2
0
4

a

a

M

5

5

5

5

3

2

1
2
0
7

1
2
0
8

1
2
1
1

1
2
1
2

1
2
1
3

H

1
2
1
4

1
2
1
6

1
2
1
5

MS-820V Closed Sampler Unit
C 1 553144 100* O-ring P-3
C 3 6114-095951A 100* Tube stopper
C 1301 534637 1 Timing belt (Polyurethane rubber)
C 1302 534655 1 Timing belt 40 S2M 126
C 1303 534673 1 Timing belt 40 S2M 184
C 1304 534887 1 Timing belt 40 S2M 256
C 1305 534992 1 Timing belt 40 S2M 328
C 1306 6114-115075B 1 Rinse unit
C 1307 6114-115084B 1 Sampling nozzle guide
C 1308 295538 1 O ring AS568-003
C 1309 315366 1 O ring AS568-004
C 1310 6114-122227C 1 Waste cup
B 1311 2114-080153A 1 Waste cup packing
B 1312 YZ-0261 1 Sampling nozzle set
B 1313 YZ-0262 1 Cap pierce nozzle set
A 1314 T802 1 Hemoglobin filter assy
D 1315 6114-028285C 1 Roller head
D 1316 6114-130397A 1 Monovette roller head
D 1317 534441 1 DC motor
D 1318 6114-114878B 1 Lead screw (MIX)
D 1319 6113-039818B 2 Sampler ball screw
C N UT-7154 1 DRIVER3 board
D O UT-7185 1 3POS SENSOR board
Rank
1312
1307
1308
1306
1309
1313
2
1314
1311
1310
1301
1302
4
4
4
1303
1305
1304
1
3
2
N
O
6
1319
6
6
1315
1318
1317
1401
1402
1407
1406
1404
1405
1403
P
1408
6
MS-821V Open Sampler Unit
C 1401 553153 1 Timing belt 40 S2M 176
C 1402 553162 1 Timing belt 40 S2M 272
C 1403 6113-034467B 1 Manual rinse unit
C 1404 6114-102418A 1 Manual rinse unit cap
C 1405 6114-108475 1 Seat spacer
C 1406 315366 1 O ring AS568-004
A 1407
YZ-0193
(T444B)
1 Manual sampling nozzle
D 1408 6113-026823C 1 Lead screw
D P UT-7065 1 SENSOR board
Rank
C
damage.
D
1502
1501
S
1503
1504
Q
R
MS-822V Table Unit
C 1501 535164 1 Timing belt 40 S2M 460
C 1502 535173 1 Timing belt 40 S2M 796
D 1503 6111-007171 1 Rack table
D 1504 6124-035659 1 Table caution label
D Q UT-7086 1 SENSOR3 board
D R UT-7157 1 TABLE SENSOR board
C S UT-7156 1 DRIVER5 board
Rank
C
damage.
D
PV-820V Display Unit
D 1601 601707 1 Color LCD
D 1602 601716 1 Touch screen
D 1603 539463 1 Inverter board
D 1604 6121-001176C 1 Display front panel
D 1605 6111-006475B 1 Display front cover
D T UT-7178 1 Front panel board set
Rank
1604
1605
1601
1602
T
T
T
1603
ZY-820V Warmer Piping Unit
C 1 553144 100* O-ring P-3
C 3 6114-095951A 100* Tube stopper
D 1901 536983 1 Tubed warmer
D U UT-7159 1 WARMER CONTROL board
Rank
4
4
1901
U
1
2
3
ZK-821V/VG CCD Bar Code Reader Unit (Option)
D 2201 6144-010933B 1 Sample tube roller
Rank
2201

Mek8222 TRM B

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