Volume 1, Issue 2, February 1999, Pages 125129 Copyright 1999 ditions scientifiques et mdicales Elsevier SAS. All rights reserved.
Abstract Allicin, one of the active principles of freshly crushed garlic homogenates, has a variety of antimicrobial activities. Allicin in its pure form was found to exhibit i) antibacterial activity against a wide range of Gram-negative and Gram-positive bacteria, including multidrug- resistant enterotoxicogenic strains of Escherichia coli; ii) antifungal activity, particularly against Candida albicans; iii) antiparasitic activity, including some major human intestinal protozoan parasites such as Entamoeba histolytica and Giardia lamblia; and iv) antiviral activity. The main antimicrobial effect of allicin is due to its chemical reaction with thiol groups of various enzymes, e.g. alcohol dehydrogenase, thioredoxin reductase, and RNA polymerase, which can affect essential metabolism of cysteine proteinase activi ty involved in the virulence of E. histolytica.
Antimicrobial properties of Allium sativum (garlic) Authors : J. C. Harris, S. Cottrell, S. Plummer, D. Lloyd Applied Microbiology and Biotechnology October 2001, Volume 57, Issue 3, pp 282-286 Abstract. Although garlic has been used for its medicinal properties for thousands of years, investigations into its mode of action are relatively recent. Garlic has a wide spectrum of actions; not only is it antibacterial, antiviral, antifungal and antiprotozoal, but it also has beneficial effects on the cardiovascular and immune systems. Resurgence in the use of natural herbal alternatives has brought the use of medicinal plants to the forefront of pharmacological investigations, and many new drugs are being discovered. This review aims to address the historical use of garlic and its sulfur chemistry, and to provide a basis for further research into its antimicrobial properties.
Natural microflora of prepeeled garlic and their resistance to garlic antimicrobial activity Authors : S.T. Shim, K.H. Kyung f1
Volume 16, Issue 2, April 1999, Pages 165172 Copyright 1999 Academic Press. All rights reserved.
Abstract Prepeeled garlic carried 10 6 -10 8 cfu g 1 micro-organisms; 10 3 -10 5 times more than freshly peeled garlic. Scanning electron micrographs revealed the presence of various types of micro-organisms on the surface of the garlic. Fifty-one lactic acid bacteria, 40 non-lactic acid bacteria and 15 yeasts were isolated from prepeeled garlic with heterofermentative lactic acid bacteria predominating among the lactic acid bacteria, andLeuconostococcurring most frequently. Twenty-six isolates of lactic acid bacteria grown in MRS broth with 20% garlic juice or higher were extremely resistant to garlic. Gram negative rod-shaped bacteria were the most frequently occurring non-lactic acid bacteria, with a predominance of the generalPseudomonasandEnterobacter. Cryptococcus neoformanswas the only species of yeast isolated. The inhibitory action of methyl methanethiosulfonate, but not of iodoacetate, appeared similar to that of garlic.
Antimicrobial activity of essential oil extracts of various onions (Allium cepa) and garlic (Allium sativum)
Author : N. Benkeblia Volume 37, Issue 2, March 2004, Pages 263268 Copyright 2003 Swiss Society of Food Science and Technology. Published by Elsevier Ltd. All rights reserved.
Abstract Antimicrobial activity of different concentrations (50, 100, 200, 300 and 500 ml/l) of essential oil extracts of three type of onions (green, yellow and red) and garlic against two bacteria, Staphylococcus aureus,Salmomella Enteritidis, and three fungi, Aspergillus niger, Penicillium cyclopium and Fusarium oxysporum, was investigated. The essential oil (EO) extracts of these Allium plants (garlic and onions) exhibited marked antibacterial activity, with garlic showing the highest inhibition and green onion the lowest. Comparatively, 50 and 100 ml/l concentrations of onions extracts were less inhibitory than 200, 300 and 500 ml/l concentrations. However, with garlic extract, high inhibitory activity was observed for all tested concentrations. S. aureus showed less sensitivity towards EO extracts inhibition, however S. Enteritidis was strongly inhibited by red onion and garlic extracts. The fungus F. oxysporum showed the lowest sensitivity towards EO extracts, whereas A. niger and P. cyclopium were significantly inhibited particularly at low concentrations. Conclusively, where seasoning is desired, essential oil extracts of onions and garlic can be used as natural antimicrobial additives for incorporating in various food products.
Antimicrobial Properties of Garlic Oil against Human Enteric Bacteria: Evaluation of Methodologies and Comparisons with Garlic Oil Sulfides and Garlic Powder
Authors : Z. M. Ross, E. A. O'Gara, D. J. Hill, H. V. Sleightholme, and D. J. Maslin *
o Received 19 June 2000. o Accepted 9 November 2000. * Corresponding author. Mailing address: University of Wolverhampton, School of Health Sciences, 62-68 Lichfield St., Wolverhampton WV1 1DJ, United Kingdom. ABSTRACT The antimicrobial effects of aqueous garlic extracts are well established but those of garlic oil (GO) are little known. Methodologies for estimating the antimicrobial activity of GO were assessed and GO, GO sulfide constituents, and garlic powder (GP) were compared in tests against human enteric bacteria. Test methodologies were identified as capable of producing underestimates of GO activity. Antimicrobial activity was greater in media lacking tryptone or cysteine, suggesting that, as for allicin, GO effects may involve sulfhydryl reactivity. All bacteria tested, which included both gram- negative and -positive bacteria and pathogenic forms, were susceptible to garlic materials. On a weight-of-product basis, 24 h MICs for GO (0.02 to 5.5 mg/ml, 62 enteric isolates) and dimethyl trisulfide (0.02 to 0.31 mg/ml, 6 enteric isolates) were lower than those for a mixture of diallyl sulfides (0.63 to 25 mg/ml, 6 enteric isolates) and for GP, which also exhibited a smaller MIC range (6.25 to 12.5 mg/ml, 29 enteric isolates). Viability time studies of GO and GP against Enterobacter aerogenes showed time- and dose-dependent effects. Based upon its thiosulfinate content, GP was more active than GO against most bacteria, although some properties of GO are identified as offering greater therapeutic potential. Further exploration of the potential of GP and GO in enteric disease control appears warranted.