AQUARIA MICROFLOW User Manual 12/08 rev.

5 page 1












USER MANUAL

MICROBIOLOGICAL
AIR SAMPLER


Microflow 60

Microflow 90-C

Microflow 60/90-C

AQUARIA MICROFLOW User Manual 12/08 rev.5 page 2
Contents

INDEX page. 2

GENERAL INFORMATION page. 3
Working principle page. 3

DESCRIPTION OF THE UNIT page. 4
Case content page. 4
Main features page. 4
Technical data page. 5
Back control panel page. 5

OPERATIVE INSTRUCTIONS page. 6
Preparing the unit page. 6
Placing the plate page. 6
Setting a sampling program page. 7
Function Sampling page. 7
Run a manual sampling page. 8
Run a programmed sampling page. 9
Run a sequential sampling page. 11
End of sampling page. 14


OTHER FUNCTIONS page. 15
Battery level page. 16
Setup date and time page. 17
Stored samplings page. 17
Download data page. 18
Delete memory page. 19
Flow rate calibration page. 20
Setup language page. 20
Setup head page. 21
Reset system page. 21



POWER SUPPLY page. 22

BATTERY CHARGER page. 22

UNIT DISINFECTION page. 24

TROUBLE SHOOTING page. 25

WARRANTY page. 26

CONVERSION TABLES page. 27

STANDARDS AND GUIDELINES page. 29

CERTIFICATES page. 30









AQUARIA MICROFLOW User Manual 12/08 rev.5 page 3
GENERAL INFORMATION

MICROFLOW has been developed for monitoring of air biocontamination level in critical places
(e.g.: clean rooms, food plants, fermentations plants, etc.). The equipment conforms to the new
European ISO/TC 209 dated 25 July 1999 recommendation.
MICROFLOW allows collection of a wide range of airborne biocontaminant by impacting on agar
standard plates (60 mm + 2 mm and 90 mm + 2 mm diameter, filled with every kind of culture
media).
MICROFLOW can be used successfully in a wide range of applications (air quality control in
pharmaceutical, food, cosmetics plants, in hospitals, clinics and laboratories) for sterile or non
sterile areas monitoring. It is very useful also for air monitoring in building air conditioning (HVAC)
plants.


Working principle

The air to be sampled is drawn through a perforated head, at constant velocity and for a period of
time depending on the condition of the area to be controlled. The inflow air impacts in the surface of
the culture media, chosen on the basis of the microbiological investigation to be carried out, placed
in a 90 mm Petri dish. At the end of the sampling period the Petri dish is removed and placed into
an incubator. At the end of the incubation it will be possible to count the CFU/cm
2
and evaluate the
air biocontamination level of the critical area on the basis of air volume sampled .










































AQUARIA MICROFLOW User Manual 12/08 rev.5 page 4
DESCRIPTION OF THE UNIT

Case content


MICROFLOW air sampler kit includes:

Carrying case
Microbiological sampler "Microflow "
60 mm aluminium adding head (inside kit
Microflow 60 , cod. G.1004 )
90-C mm aluminium adding head (inside kit
Microflow 90-C, cod. G.1015)
60 or 90 mm aluminium head assembly
(inside kit Microflow 60/90-C, G.1027)
1 pack of 10 PCA Rodac plates
(only inside kit Microflow 60 e 60/90-C, cod. G.1004 e G.1027)
Battery charger with full charge indicator
I.R. remote control
Pencil torch
1 pack of 10 Contact plates (only Microflow 60 and 60-C and Microflow 60/90-C)
User manual in English
Calibration Report
Warranty card


Main features

Used with: contact plates 60 + 2 mm (Microflow 60)
Petri plates 90 + 2 mm (Microflow 90)
the same instrument allows to sample with 60 or 90 mm plates (Microflow 60/90-C)
Rechargeable battery operated. Battery Ni/Mh (memory effect free)
Silent electric fan microprocessor controlled to assure the maximum reliability of the air volume
sampled
Compensation airflow system (models compensated)
Low battery control and alarm
User friendly. Prompts in 5 languages: English, French, German, Spanish, Italian. All functions
are visualised step-by-step.
Delay start
Total volume of sampled air programmable from 1 up to 2000 litres, with 1 litre steps
Manual sampling (for air volumes greater than 2000 litres)
Sequential sampling also available
Storing and visualising up to 99 samplings, with record of progressive number, date, hour,
volume or time of sampling, flow rate, type of sampling (manual, sequential, programmable).
USB port to download data to a PC (optional)
Calibration control function (with optional calibration kit)
Remote switch with infra-red rays control
Two sampling positions (90-180) and built-in soc ket for tripod








AQUARIA MICROFLOW User Manual 12/08 rev.5 page 5

Technical data

Dimensions: 310 x 130 x 170 mm (W x D x H)
Weight: 1,9 Kg
Case: Polyurethane, joint-free, easy to disinfect
60 mm head: Autoclavable anodised aluminium head, with 219 holes ( 1 mm)
90 mm head: Autoclavable anodised aluminium head, with 380 holes ( 1 mm)
Plate support: Autoclavable anodised aluminium plate support for 60 and 90 mm 2 mm
plates.
Flow rates: 30 60 90 - 100 (ISO/TC 209) -120 l/min
Sampled Air Volume: 1 - 2000 litres (and more with manual control)



Back control panel



















- ON/OFF button
This is the power switch.
Put in the ON position to power the unit.
Place on OFF at the end of the use to save the battery charge.

- Data Output
USB port (optional) to transfer on the PC the stored data

- Battery charger connector

- Remote control infra-red sensor

- Display
High contrast graphic display matrix

- Start/Stop; Prog; Clear; Set ; Enter ; Up ; Down.
Programming multifunctional keys

Fig. 1 - Control panel

AQUARIA MICROFLOW User Manual 12/08 rev.5 page 6
OPERATIVE INSTRUCTIONS

Preparing the unit

Before to start the sampling, MICROFLOW must be disinfected, programmed, loaded with a plate
and put on a flat surface or on a suitable photographic tripod (avoid to screw too hard).

WARNING: dont screw too hard the tripod to avoid demage

MICROFLOW allows to operate with 60 + 2 mm or 90 mm + 2 mm AGAR PLATES. It depends
on the head assembly chosen.

Before to operate its necessary to set the right plate diameter of the aluminium plate support. This
setting will not be modified until the change of the type or brand of Petri dishes used for the
sampling.


















Placing the plate

Wear sterile gloves. Remove the sampling head handling with care to avoid any contamination of
its surfaces. Deposit the head on a clean surface.

Disinfect the aluminium plate support with a suitable disinfectant, avoiding liquid spillage inside the
instrument.

Place a sterile filled plate on the first aluminium plate
support. Press gently in outer direction and lay the
plate on the others (see figure).Remove the dish lid,
and deposit it on a clean surface.
Close the sampling unit screwing clockwise the
sterilised aluminium head on the plate support.





Aluminium plate
support
Screw
Screw
Plate holders

AQUARIA MICROFLOW User Manual 12/08 rev.5 page 7
Setting a sampling program

Switch on the unit by pressing ON push button.

On the display will appear sequentially the following start information:



























FUNCTION: "Sampling"

Press [Start] key to start a sampling using the last stored parameters.

WARNING: using this procedure the delay start is not considered

Press [Prog] key to enter a new sampling program.

Press [Up] and [Down] key to display the following indications :













When the needed sampling is shown, press [Enter].

MICROFLOW 90
Version 3.0.0
01/05/09 12:00
Serial Number
PRG Program
SET Setup
START Start
Level 100%

Sampl i ngs Type:
Manual
change
ENTER sel ect
Sampl i ngs Type:
Programmed
change
ENTER sel ect
Sampl i ngs Type:
Sequent i al
change
ENTER sel ect
COMPANY NAME
Address
Address
Address
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 8

Run a Manual sampling

The operator can run a manual sampling with times or volumes different from the preset by the
manual equipment control.

To program the Microflow on a manual sampling, display:








and Press [Enter] key.

It will appear:




Where xxx is the last selected flow rate.


If the flow rate is correct, press [Enter]. If not, select the needed flow rate using the [Up] and
[Down] keys. When the flow rate needed is displayed press [Enter].

It will appear:


Where:
Manual Samp.= manual sampling selected
xxx is the needed flow rate.


If the programmed data sampling are correct, press [Enter] and it will appear the sequence of the
three initial displays. If it is necessary to change some data, press the [Clear] key and the first
display (Manual sampling) will appear.

Press [Start] key to start the sampling. The two red LED will switch on and, on the display, it will
appear:

Where:
t = sampling time
L= sampling volume
Fr = selected flow rate


Press [Stop] key to end the sampling. The two red LED will switch off and, on the display, it will
appear the following message:








A beep will continue until it will not be pressed [ENTER] to store the sampling data or [CLEAR] to
exit without storing .
Sampl i ng t ype:
Manual
change
ENTER sel ect
Fl ow Rat e
xxx l / mi n
change
ENTER sel ect
Manual Sampl i ng

xxx l / mi n
ENTER sel ect
Sampl i ng end
Fr xxx t hh: mm: ss
ENTER esc
CLEAR del et e
Manual sampl i ng
t hh: mm: ss
L xxxx. x Fr xxx

AQUARIA MICROFLOW User Manual 12/08 rev.5 page 9

Run a programmed sampling

To program the Microflow on a programmed sampling, display:









and Press [Enter] key.


It will appear:




xxxx is the last selected volume.




If the volume is correct, press [Enter]. If not, select the needed volume using the [Up] and [Down]
keys, to increase or decrease the value, and the [Set] key to move the cursor.

WARNING: Programmed mode allows to sample air volume between 1 and 2000
Lt. To sample greater air volumes it is necessary to work in Manual
mode.

When the volume needed is displayed press [Enter].

It will appear:





xxx is the last flow rate selected.



If the flow rate is correct, press [Enter]. If not, select the needed flow rate using the [Up] and
[Down] keys. When the flow rate needed is displayed press [Enter].

It will appear:








Set the needed delay using the [Up] and [Down] keys, to increase or decrease the value, and the
[Set] key to move the cursor. When the delay needed is displayed press [Enter].
Sampl i ng t ype:
Programmed
change
ENTER sel ect
Vol ume
L xxxx
change
ENTER sel ect
Fl ow Rat e
xxx l / mi n
change
ENTER sel ect
Del ay St art
00 : 00
change
ENTER sel ect
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 10
WARNING: Set EXACTLY delay time (hour an minutes) for the beginning of the
sampling.
If the delay start is not needed, leave 00:00


After the [Enter] key has been pressed, all the parameters will be displayed (Volume, Flow rate,
Sampling time calculated on the volume and flow rate basis, and the eventual delay start)


Where:
Fr = set flow rate (L/min.)
V = sampling volume (Litres)
Ds = delay start
t = sampling duration (the sampling time calculation
is based on the flow rate and volume set)

If the set data are correct, press [Enter] to save the program.
The three first displays will appear.

If it is necessary to change one or more data, press the [Clear] key and it will be shown the initial
display with the programmed sampling settings. Press the [Enter] key up to the parameter that it is
necessary to change. Modify the parameter and press [Enter] to save the setting. Press [Enter]
up to the summing up screen and save the set parameters pressing the [Enter] key.

The three initial displays will appear and the Microflow is ready for the sampling.
Press [Start] key to start the sampling. The two red LED will switch on and, on the display, it will
appear:


a) The delay start is not active:

The two red LED will brighten and, on the display, will be shown:



and then






At the end of the sampling period the red LED will go out and the following message will
appear:











At the same time a beep will inform the user that the sampling is finished. A beep will
continue until it will not be pressed [ENTER] to store the sampling data or [CLEAR] to exit
without storing .




Fr xxx l / mi n
V yyyy L
Ds 00: 00 t mm: ss
ENTER sel ect
Program sampl i ng
wai t
st art

Program sampl i ng
t hh: mm: ss
L xxxx. x Fr xxx

Sampl i ng end
Fr xxx L xxxx. x
ENTER esc
CLEAR del et e
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 11

b) The delay start is activated

The two red LED will lighten and, on the display, will be shown:








At the end of the delay start the LED will brighten and, on the display, will be shown:




and then





At the end of the sampling period the red LED will go out and the following message
will appear.










At the same time a beep will inform the user that the sampling is terminated.The beep will
continue until it will not be pressed [ENTER] to store the sampling data or [CLEAR] to exit
without storing .


Run a sequential sampling

To program the Microflow on a sequential sampling, display:









and Press [Enter] key.

It will appear:



xxxx is the last selected volume.



Wai t pl ease
Del ay St art
hh : mm : ss

Sampl i ng t ype:
Sequent i al
change
ENTER sel ect
Vol ume
L xxxx
change
ENTER sel ect
Program sampl i ng
wai t
st art

Program sampl i ng
t hh: mm: ss
L xxxx. x Fr xxx

Sampl i ng end
Fr xxx L xxxx. x
ENTER esc
CLEAR del et e
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 12

If the volume is correct, press [Enter]. If not, select the needed volume using the [Up] and [Down]
keys, to increase or decrease the value, and the [Set] key to move the cursor.


WARNING: The Seqential mode allows ONLY samplings with air volume between
1 and 2000 litres


When the needed volume is displayed press [Enter] key.

It will appear:



xxx is the last flow rate selected.




If the flow rate is correct, press [Enter]. If not, select the needed flow rate using the [Up] and
[Down] keys. When the flow rate needed is displayed press [Enter].



It will appear:

Where:

NUM SQ = number of sequential samplings

BreakSQ = break (minutes) between two
samplings


Set the needed data using the [Up] and [Down] keys, to increase or decrease the value, and the
[Set] key to move the cursor.


WARNING: It is possible to set from 1 up to 99 cycles
It is possible to set a break between 1 and 120 minutes max.


When the sequence needed is displayed press [Enter].

It will appear:









Set the needed delay start for the first sampling using the [Up] and [Down] keys, to increase or
decrease the value, and the [Set] key to move the cursor. When the delay needed is displayed
press [Enter].


Fl ow Rat e
xxx l / mi n
change
ENTER sel ect
NUM SQ 01
BreakSQ 001 mi n
change
ENTER sel ect
Del ay St art
00 : 00
change
ENTER sel ect
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 13
WARNING: Set EXACTLY delay time (hour an minutes) for the beginning of the
sampling
If the delay start is not needed, leave 00:00


After the [Enter] key has been pressed, all the parameters will be displayed (Flow rate, volume, the
eventual delay start, sampling time calculated on the volume and flow rate basis, number of setting
sequences, time between sequences).








Where:
xxx = set flow rate (L/min.)
yyyy = sampling volume (Litres)
Ds 00:00 = Delay start
t mm:ss = sampling duration in seconds (the sampling time calculation is based
on the flow rate and volume set)
Nseq nn = number of sequences
Bseq sss = break between a sampling and the following (seconds)

If the set data are correct, press [Enter] to save the program.
The three first displays will appear.
If it is necessary to change one or more data, press the [Clear] key and the it will be shown the
initial display with the programmed sampling settings. Press the [Enter] key up to the parameter
that it is necessary to change. Modify the parameter and press [Enter] to save the setting. Press
[Enter] up to the summing up screen and save the set parameters pressing the [Enter] key.

The three initial displays will appear and the Microflow is ready for the sampling.

Press [Start] and the Microflow will start the sampling as shown for the programmed sampling.

It will appear:









During sampling:

t = countdown end sampling (h/m/s)
L = sampling volume (Litres)
Sq = sampling number respect the numbers
sampling set (es : 01/02 says that we are
doing the first sampling up two)


In this moment the LED will switch on.





Fr xxx l / mi n
V yyyy
Ds 00: 00 t mm: ss
Nseq nn Bseq sss
Sequen. sampl i ng
t hh: mm: ss
L xxxx. x Sq01/ NN

Sequent i al
sampl i ng
wai t
st art
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 14
Between two sequences sampling:


Sq nn/NN = n sampling to do over the total
sampling to do (es : 02/02 says that we
are waiting for beginning the second
sampling up two)
t = countdown end sampling (h/m/s)


In this moment the LED will brighten.


During the following sampling:



Only the number of sequences changes





In this moment the LED will switch on.

At the end of the sampling period the red LED will go out and then it will appear:











At the same time a beep will inform the user that the sampling is terminated. The beep will continue
until it will not be pressed [ENTER] to store the sampling data or [CLEAR] to exit without storing .



End of sampling

Switch off the Microflow pressing the [Off] key.

Remove the sampling head unscrewing anticlockwise.

Close the plate with the lid, and after the identification, bring it to incubation.

Prepare the unit to the next sampling.

WARNING: In order to save energy when MICROFLOW is waiting for a new
sampling, after about 10 minutes of its inactivity the LCD display
lighting system automatically switch off, it will appear the indication
Stand by, until ENTER key is pressed.



Sequen. sampl i ng
t hh: mm: ss
Next sampl i ng
Sq nn/ NN
Sequen. sampl i ng
t hh: mm: ss
L xxxx. x Sq02/ NN

Sampl i ng end
Fr xxx Sq nn/ NN
ENTER esc
CLEAR del et e
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 15
OTHER FUNCTIONS

Press [on/off] to switch on the instrument.

Press [Set] to enter in the setting menu of the MICROFLOW .

Press the [Up] key to scroll the following menus on the Display:



























Press [Enter] to enter in a menu.


In the Microflow 60/90-C (cod. G.1069), and ONLY in this instrument, after the display







It will appear:








Bat t ery l evel

change
ENTER sel ect
Set up
dat e and cl ock
change
ENTER sel ect
St ored sampl i ngs

change
ENTER sel ect
Del et e memory

change
ENTER sel ect
Fl ow rat e
cal i brat i on
change
ENTER sel ect
Language Set up

change
ENTER sel ect
Reset Syst em

change
ENTER sel ect
Set up
Language
change
ENTER sel ect
Set up head

change
ENTER sel ect
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 16
When inside the instrument is fitted the download data system (cod. G1117), after the display








It will appear:












FUNCTION: "Battery level"

In the battery level menu:









Press [Enter] and it will appear while the fun switch on:



xxx % battery level

000 % means that the instrument isnt under
recharge



The battery level is shown by a number (%) on by the wording HIGH, WARNING and LOW .

The word WARNING will appear when the level is around 40%, in this case we suggest to recharge
the battery because the instrument will be able to sample for about 20 minutes, lower is the
percentage and lower is the possible working time .
The word LOW will appear when the level arrives to 5%, in this case you have to recharge the
battery before use the instrument, you can only press the [ON/OFF] key.

AQUARIA RECOMMEND TO CHARGE THE BATTERY WHEN IT
ARRIVES IN WARNING LEVEL.

Press the [Clear] key to exit from the Battery level menu.

Press again the [Clear] key to visualize the first three displays.
Downl oaded dat a

change
ENTER sel ect
Level HI GH
xxx%
Charge current
000%
St ored sampl i ngs

change
ENTER sel ect
Bat t ery Level

change
ENTER sel ect
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 17
FUNCTION: "Setup date and clock"


In the Setup date and clock menu,










Press [Enter] after the visualisation and it will appear:










Set data and time using the [Up] and [Down] keys, to increase or decrease the value, and the
[Set] key to move the cursor.

Press the [Clear] key to exit from the Setup date and clock menu.
Press one time the [Clear] key to stay in the Set menu. Press two times the [Clear] key to visualize
the first three displays.


FUNCTION: "Stored samplings"

In the Stored samplings menu:










Press [Enter] after the visualisation an it will appear:


a) Manual sampling

Nxxx = sampling sequential number
dd/mm/yy = sampling date (day/month/year)
hh:mm = sampling time (hour/minutes)
Fr = yyy = flow rate (L/min)
H = sampling head
t= wwww = sampling duration (seconds)




Dat a 25/ 02/ 07
Hour 12: 42: 03
change
ENTER sel ect
Set up
dat e and cl ock
change
ENTER sel ect
St ored sampl i ng

change
ENTER sel ect
N xxx
dd/ mm/ yy hh: mm
Fryyy l / mi n H 90
t wwww sec
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 18
b) Programmed sampling


Nxxx = sampling sequential number
dd/mm/yy = sampling date (day/month/year)
hh:mm = sampling time (hour/minutes)
Fr = yyy = flow rate (L/min)
H = sampling head
V=zzzz = air volume sampled (Litres)


c) Sequential sampling

Where:
Nxxx = number of sampling
dd/mm/yy = sampling date (day/month/year) of t
hh:mm = sampling time (hour/minutes)
Seq = sequential sampling
Fr=yyy = flow rate (L/min)
H = sampling head
V=zzzz = air volume sampled (Litres)
01 of 02 = the first sampling of a sequence of 2
samplings
02 of 02 = the second sampling of a sequence
of 2 samplings
IS xxx =delay start between sequences of
sampling

Press the [Clear] key to exit from the Stored samplings menu.

Press one time the [Clear)] key to stay in the Set menu. Press two times the [Clear] key to
visualise the first two displays.

FUNCTION: "Download data "
(only for Microflow with PC interface installed)

In the Download data menu:




Tx = numbers of samplings to download




Press [Enter] after the visualisation and it will appear:









After the download it will appear automatically the set menu. Press the [Clear] key to visualise the
first three displays.

N xxx Seq 01/ 02
dd/ mm/ yy hh: mm
Fryyy l / mi n H 90
V zzzz l I S xxx
N xxx
dd/ mm/ yy hh: mm
Fryyy l / mi n H 90
V zzzz l
Downl oad Dat a

change
ENTER sel ect
Wai t pl ease
Downl oad dat a
Tx 002/ 002
Downl oaded Dat a
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 19
NOTE: during this step, the Microflow has to be connected to a PC with the proper cable. We
suggest to download only after the connection with the PC.
WARNING: Every time the download is done, the Microflow transfer all the data to the
PC. We suggest to erase the data stored on the Microflow after the
download.

FUNCTION: "Delete memory"

In the Delete memory menu








Press [Enter] after the visualisation and it will appear:








Select the starting point of deletion of samplings memorized
Press the [Up] and [Down] keys to scroll the sampling number.
Use [Set] to move the cursor to select the end point of deletion of samplings memorized
Press the [Up] and [Down] keys to scroll the sampling number.

Press [Enter] after the visualisation and it will appear:








If it is not necessary to delete the stored data press the [Enter] key to exit from this menu.
If it is necessary to delete the data press the [Down] key and it will appear:








Press the [Enter] key to delete all the stored data an it will appear








After waiting the time for the memory reset, it will appear automatically the set menu. Press the
[Clear] key to visualise the first three displays.
Del et e memory

change
ENTER sel ect
Are you sure ?
No
Are you sure ?
Yes
Erase
memory
Del et e
sampl i ngs
i n memory
f rom 001 t o 001
Del et e
sampl i ngs
i n memory
f rom 001 t o 001
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 20
FUNCTION: "Flow rate calibration"

In the Flow rate calibration menu:









Press [Enter] after the visualisation and It will appear:








In order to avoid an undesired cancellation of the calibration, the password
will be supplied ONLY at the customers who buy the MICROCONTROL II
calibration kit.
Press the [Enter] key to exit from the Flow rate calibration menu.

Press one time the [Enter] key to stay in the Set menu. Press the [Clear] key to visualise the first
three displays.

FUNCTION: " Language Setup "

In the Language Setup menu:









Press [Enter] after the visualisation and It will appear:









Press the [Up] and [Down] keys to scroll the available languages (ITALIANO, ENGLISH,
FRANCAIS, DEUTSCH, ESPANOL).

Press [Enter] to confirm.
After the confirmation, it will appear automatically the set menu. Press the [Clear] key to visualise
the first three displays.
Password
0000
Fl ow rat e
cal i brat i on
change
ENTER sel ect
Language Set up
ENGLI SH
Language Set up

change
ENTER sel ect
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 21
FUNCTION: "Setup head"
(only for Microflow 60/90-C)

In the Setup head menu:








Press [Enter] after the visualisation and it will appear:






Press the [Up] and [Down] keys to scroll the available heads: 60 and 90.
Press [Enter] and the following display will appear:







Press the [Up] and [Down] keys to choose yes or no and confirm with [Enter] key.

The Setup head menu will be displayed again. Press the [Clear] key to come back to the main
menu.

FUNCTION: "Reset system"

In the reset system menu:









Press [Enter] after the visualisation and it will appear:








This menu can be only used by the manufacturer.

Press two times the [Enter] key to visualise the first three displays.
Password
0000
Reset Syst em

change
ENTER sel ect
Set up head

change
ENTER sel ect
Head
90
change
ENTER sel ect
Are you sure ?
No
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 22
POWER SUPPLY


The unit is powered by a 8 elements Ni/MH battery pack (each element: 1,2V) protect against
short-circuits and termic overcharges.

NOTE: These batteries have no memory effect and can be recharged at any time without
damaging the elements

The chip installed on the instrument avoid to run a sampling if the battery level is not enough to
maintain a constant flow rate as long as necessary


BATTERY CHARGER

Discharge battery

a) Sampler switch on but not operative

If the MICROFLOW has been left switched on, although not operating, the battery level will slowly
decrease. If the level decrease over the limit WARNING, will appear:








b) Sampler operative
If MICROFLOW is sampling and during the sampling the battery charge decreases under the
WARNING level (and it is not possible to maintain the constant flow rate), the display will show:


Fr: Flowrate setted
T : Past sampling time



Push the button [ Enter ] the sampling will be stored
Push the button [ Clear ] the sampling will not be stored

ATTENTION : The sampling will not be stored on the basis of the values setted but on the basis of
the real sampling time

After pressing the button [ Enter / Clear ] will appear :







and its not possible proceed with other samplings until the instrument will not be charge.
Bat t ery
Level
Very l ow
WARNI NG
Bat t ery
Level
Very l ow
WARNI NG

Sampl i ng end
Fr xxx t hh: mm: ss
ENTER
CLEAR
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 23

Battery charge

ATTENTION : to avoid any damages to batteries or power adapter we suggest to follow the
following sequence:

- Connect recharger jack to Microflow
- Connect the charger the power supply

After the recharge, disconnect the charger to the power supply then disconnect the jack from the
instrument.

The complete recharging time is about 4-5 hours but it depends on the residual battery charge.

ATTENTION : we recommend to recharge the batteries when the instrument is off.
You can also recharge the instrument switched on but, in this case the charging time could be
longer and the full battery recharge is not guaranteed.

If the Microflow remains connected to the power for more than 8 hours, the charger automatically
switches off.

- Instrument OFF

The charging of the instrument may be followed by observing the LED on the instrument

LED flash rapid: the instrument is in recharge

LED flash slow : the instrument is fully charged (battery level 100%) and in maintenance phase

LED off : the instrument is fully charged (battery level 100%)


- Instrument ON

The LED will be switched off if the instrument is switched on but not active and will display the word
"Battery charging"

ATTENTION : If necessary the Microflow can also be used connected to the power supply
through the battery charger

During the recharging, you can enter the menu and select "level battery charge", on the display will
appear both the charge percentage and the current recharge percentage.
If the charger is connected to the instrument, the battery charge level showed on the display will be
100% (this means that the battery is charging at 100%) while the current recharge will be 100% at
the beginning but it will decrease during the battery charge.
If the charger is not connected to the power supply the current recharge will be equal to 0% (any
current recharge is issued) and the battery charge real percentage value will be displayed.

ATTENTION : To avoid the total batteries discharge, under the threshold value, that would
cause a very long recharge battery time (also over 10 hours), we recommend to recharge the
batteries at least once a month even if the instrument has not been used and in any case, to
recharge always the instrument after the utilisation .

Replacement batteries
To change the batteries is enough to unscrew the metal panel placed at the bottom of the
instrument.
Be careful to avoid lifting the lid to detach the lead from the IR sensor (if something happens
reconnect the cable paying attention to the color of wires).
The batteries are joined to the battery holder by a velcro strip : remove the batteries, disconnect the
battery connector and replaced the batteries.
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 24
Head Control - valve
UNIT DISINFECTION


MICROFLOW must be cleaned and disinfected after each sampling cycle to avoid cross
contamination which could modify the reliability of the sampling.

Switch off the unit.

Clean the surface of MICROFLOW using a sterile cloth wet with a suitable disinfectant. After
drying, the unit is ready for a new sampling cycle.

WARNING: DONT SPRAY disinfectant solution directly on the instrument or the
keyboard: these solutions could enter inside the instrument and they
cause damage as example and oxidation of the circuits.

AQUARIA WILL NOT RECOGNIZE THE WARRANTY CAUSED OF
UNSUITABLE DISINFECTANT SOLUTION


The sampling head and the support plate can be sterilized in autoclave, following the standard
procedure of sterilisation ( i.e. 120 C, 18 minute s).

Between two samplings is necessary to disinfect the sampling head with pencil torch supplied with
the starting kit. In the absence of this, it is possible to use a sterile cloth with disinfectant over this. It
is very important to avoid the liquid penetration inside the instrument.

Let dry at room temperature. After drying the sampling head is ready to be used.

Sampling head fire disinfecting

The MICROFLOW kit is equipped with a pencil to disinfect the head. Disinfect always the head
between two samplings to avoid a cross-contamination.











To switch on the pencil torch rotate clockwise the control-valve. When the gas goes out
from the head, bring the fire to light the pencil.

WARNING: Pass quickly the fire on the interior part of the sampling head.
Do not stop the fire on a specific point of the head, it can be damaged.

To switch off the pencil torch rotate counter-clockwise the control-valve.

To recharge the pencil torch it is possible to use the Butan gas used for cigarette-lighters.

Before to recharge the pencil torch be sure it is switched off. The complete recharge needs about
10 seconds. It is necessary to wait some minutes so that the gas is stable.

For correct recharging it is recommended to keep horizontal the pencil torch and the and the
recharge cartridge
P E N C I L T O R C H
AQUARIA MICROFLOW User Manual 12/08 rev.5 page 25
MAINTENANCE

All operations shall be performed by qualified personnel only!

There are no parts replaceable by the user himself. In case of problems please call your dealer for
service.

WARNING: It is recommended to calibrate the instrument at least once a
year


TROUBLE SHOOTING

In the unit nothing is switched on Verify:
1. The unit is switch on
2. The battery is charged
3. If doesnt work again, please call for service.

The blower motor does not work Please call for service

The infrared remote control switch doesnt
start the sampling
Verify:
1. Infrared remote switch battery is charged
2. If doesnt work again, please call for service.

LCD display doesnt work properly

Please call for service.
LCD display shows question marks or other
characters

Please call for service.
Red LED doesnt light during the sampling

Please call for service.
Blower motor make noise Verify:
1. Presence of foreign bodies inside the fan
2. If noise still persists, please call for service

Fan velocity is out of any control 1. If the problem is present for a flow rate only,
calibrate that flow rate with the calibration kit
following related instructions.
2. If the problem is present for all flow rates,
verify each flow rate calibration
3. If the model is 60/90, take off the head and
the support plate. Verify the alignment of the
fan
4. If the problem still persists, please call for
service.

Soft touch control panel doesnt work. Please call for service.

AQUARIA MICROFLOW User Manual 12/08 rev.5 page 26
WARRANTY



AQUARIA provides 1 year warranty on the instrument starting on the date of purchase.

Within the warranty period Aquaria will eliminate, free of charge, any defects in the MICROFLOW
microbiological air sampler resulting from failures in material or workmanship either by repairing, or
exchanging parts or exchanging the whole appliance as Aquaria may choose.

Damages caused by improper use as well as negligence to use the instrument, normal
deterioration of internal parts, some defects with negligible effects on the ordinary function of the
instrument are not included in this warranty.

This warranty declines in case of technical intervention repairs which are carried out by non
authorized personnel.

Aquaria is not ever responsible, in front of the purchaser or end user, for damage caused by people
or things (included the same instrument) in relation to the functioning of the instrument, that it is
caused by:

a) any type of work or modification carried out inside the instrument unit, or its accessories,
without Aquaria previous authorization and /or Aquaria technical direct instructions, even in
the case that such works are carried out by Aquaria employees, but without Aquaria proper
authorization.

b) to use the MICROFLOW and/or its accessories for purposes which are different from the
original purposes indicated by Aquaria at the time of planning the design, manufacture and
sell the instrument

c) to clean MICROFLOW and/or its accessories with chemical products different from the
Aquaria indications and /or to use Microflow under different operational conditions as
indicated by Aquaria


For further information concerning Microflow please contact:












For technical assistance please contact:








AQUARIA srl Via della Levata, 14
20084 - Lacchiarella, Milan (Italy)
Tel +39-02-90091399 - FAX +39-02-9054861
E-mail: info@aquariasrl.com

AQUARIA srl - Via della Levata, 14
20084 - Lacchiarella, Milan (Italy)
Tel +39-02-90091399- FAX +39-02-9054861
E-mail: assistenza@aquariasrl.com

AQUARIA MICROFLOW User Manual 12/08 rev.5 page 27
CONVERSION TABLES

To correlate the CFUs (Colony Forming Units) present on the agar plate to the most probable
number (MPN) of micro-organisms per cubic meter of air sampled, the following formula is used:

P
r
= N [1/N + 1/(N-1) + 1/(N-2) + ... + 1/N-(r+1)]

Where:
P
r
= most probable number of micro-organisms in the volume of air sampled
N = number of holes on Microflow sampling head
R = number of CFUs on the agar plates after incubation

How to calculate a result:

To calculate the correct result, following data are necessary:
- Chosen sampling volume on Microflow (litres)
- Number o colonies on the agar plate after incubation (cfu)
- Corrected number of colonies from tables below (cfu)

Number of cfu/m
3
= Corrected number of colonies from tables below (cfu) x 1000
Chosen sampling volume on Microflow (litres)

CONVERSION TABLE Microflow 60

r P
r
r P
r
r P
r
r P
r
r P
r
r P
r
r P
r
r P
r
r P
r

1 1 27 29 53 61 79 98 105 143 131 199 157 275 183 393 209 666
2 2 28 30 54 62 80 99 106 144 132 201 158 279 184 399 210 688
3 3 29 31 55 63 81 101 107 146 133 204 159 282 185 405 211 712
4 4 30 32 56 65 82 102 108 148 134 206 160 286 186 412 212 739
5 5 31 33 57 66 83 104 109 150 135 209 161 290 187 418 213 771
6 6 32 35 58 67 84 106 110 152 136 212 162 293 188 425 214 807
7 7 33 36 59 69 85 107 111 154 137 214 163 297 189 432 215 851
8 8 34 37 60 70 86 109 112 156 138 217 164 301 190 440 216 906
9 9 35 38 61 71 87 111 113 158 139 220 165 305 191 447 217 979
10 10 36 39 62 73 88 112 114 160 140 222 166 309 192 455 218 1.088
11 11 37 40 63 74 89 114 115 163 141 225 167 313 193 463 219 1.307
12 12 38 42 64 75 90 116 116 165 142 228 168 317 194 471
13 13 39 43 65 77 91 117 117 167 143 231 169 322 195 480
14 14 40 44 66 78 92 119 118 169 144 234 170 326 196 489
15 16 41 45 67 80 93 121 119 171 145 237 171 331 197 499
16 17 42 47 68 81 94 122 120 173 146 240 172 335 198 509
17 18 43 48 69 83 95 124 121 175 147 243 173 340 199 519
18 19 44 49 70 84 96 126 122 178 148 246 174 345 200 530
19 20 45 50 71 86 97 128 123 180 149 249 175 349 201 542
20 21 46 52 72 87 98 130 124 182 150 252 176 354 202 554
21 22 47 53 73 89 99 131 125 185 151 255 177 360 203 567
22 23 48 54 74 90 100 133 126 187 152 258 178 365 204 580
23 24 49 55 75 92 101 135 127 189 153 261 179 370 205 595
24 25 50 57 76 93 102 102 128 192 154 265 180 376 206 611
25 26 51 58 77 95 103 139 129 194 155 268 181 381 207 628
26 28 52 59 78 96 104 141 130 196 156 272 182 387 208 646


AQUARIA MICROFLOW User Manual 12/08 rev.5 page 28
CONVERSION TABLE Microflow 90



r P
r
r P
r
r P
r
r P
r
r P
r
r P
r
r P
r
r P
r
r P
r


1 1 46 49 91 104 136 168 181 245 226 342 271 473 316 674 361 1129
2 2 47 50 92 105 137 170 182 247 227 345 272 477 317 680 362 1149
3 3 48 51 93 106 138 171 183 249 228 347 273 480 318 686 363 1170
4 4 49 52 94 108 139 173 184 251 229 350 274 484 319 693 364 1192
5 5 50 54 95 109 140 174 185 253 230 352 275 487 320 699 365 1216
6 6 51 55 96 110 141 176 186 255 231 355 276 491 321 705 366 1242
7 7 52 56 97 112 142 178 187 257 232 358 277 495 322 712 367 1269
8 8 53 57 98 113 143 179 188 259 233 360 278 498 323 718 368 1298
9 9 54 58 99 115 144 181 189 261 234 363 279 502 324 725 369 1330
10 10 55 59 100 116 145 182 190 263 235 365 280 506 325 732 370 1364
11 11 56 60 101 117 146 184 191 265 236 368 281 510 326 738 371 1402
12 12 57 62 102 119 147 186 192 267 237 371 282 514 327 745 372 1444
13 13 58 63 103 120 148 187 193 269 238 373 283 517 328 753 373 1492
14 14 59 64 104 121 149 189 194 271 239 376 284 521 329 760 374 1546
15 15 60 65 105 123 150 190 195 273 240 379 285 525 330 767 375 1609
16 16 61 66 106 124 151 192 196 275 241 381 286 529 331 775 376 1685
17 17 62 68 107 125 152 194 197 277 242 384 287 533 332 783 377 1780
18 18 63 69 108 127 153 195 198 279 243 387 288 537 333 791 378 1907
19 19 64 70 109 128 154 197 199 281 244 390 289 542 334 799 379 2097
20 21 65 71 110 130 155 199 200 283 245 392 290 546 335 807 380 2477
21 22 66 72 111 131 156 200 201 285 246 395 291 550 336 815
22 23 67 74 112 132 157 202 202 288 247 398 292 554 337 824
23 24 68 75 113 134 158 204 203 290 248 401 293 559 338 833
24 25 69 76 114 135 159 206 204 292 249 404 294 563 339 842
25 26 70 77 115 137 160 207 205 294 250 407 295 567 340 851
26 27 71 78 116 138 161 209 206 296 251 410 296 572 341 861
27 28 72 80 117 140 162 211 207 298 252 413 297 576 342 871
28 29 73 81 118 141 163 213 208 301 253 415 298 581 343 881
29 30 74 82 119 143 164 214 209 303 254 418 299 586 344 891
30 31 75 83 120 144 165 216 210 305 255 421 300 590 345 901
31 32 76 85 121 145 166 218 211 307 256 425 301 595 346 912
32 33 77 86 122 147 167 220 212 310 257 428 302 600 347 923
33 34 78 87 123 148 168 221 213 312 258 431 303 605 348 935
34 36 79 88 124 150 169 223 214 314 259 434 304 610 349 947
35 37 80 90 125 151 170 225 215 316 260 437 305 615 350 959
36 38 81 91 126 153 171 227 216 319 261 440 306 620 351 972
37 39 82 92 127 154 172 229 217 321 262 443 307 625 352 985
38 40 83 94 128 156 173 230 218 323 263 447 308 630 353 998
39 41 84 95 129 157 174 232 219 326 264 450 309 635 354 1012
40 42 85 96 130 159 175 234 220 328 265 453 310 641 355 1027
41 43 86 97 131 160 176 236 221 330 266 456 311 646 356 1042
42 44 87 99 132 162 177 238 222 333 267 460 312 652 357 1058
43 46 88 100 133 163 178 240 223 335 268 463 313 657 358 1075
44 47 89 101 134 165 179 242 224 338 269 466 314 663 359 1092
45 48 90 103 135 167 180 243 225 340 270 470 315 669 360 1110










AQUARIA MICROFLOW User Manual 12/08 rev.5 page 29
STANDARDS AND GUIDELINES


Standards and guidelines (pharmaceutical industry):

Cleanroom classification acc. To EU GMP guidelines (for sterile products) valid from January 1,
1997

Cleanroom
Class
Max. Number Of Particles/m
3
= 0,5 m Max. Number Of Particles/m
3
= 5 m
A 3.500 0
B 350.000 2.000
C 3.500.000 20.000
D - -


Cleanroom classification acc. To Federal Standard 209E (United States); USP

Cleanroom
Class
Max. Number Of Particles/m
3
= 0,5 m Max. Number Of Particles/m
3
= 5 m
100 3.530 -
10.000 353.000 2.470
100.000 3.530.000 24.700


Microbial limits:

Microbial classification acc. To EU GMP guidelines (for sterile products) valid from January 1,
1997

Cleanroom
Class
cfu/m
3
air Petri plate ( 90 mm);
cfu / 4 h
Rodac plate ( 55 mm);
cfu / 4 h
Finger print;
5 finger cfu
A < 1 < 1 < 1 < 1
B 10 5 5 5
C 100 50 25
D 200 100 50 -

Acc. To draft USP chapter <1116>

Cleanroom
Class
cfu/m
3
air Surface cfu /
24 cm
2
Personell, gloves
cfu / 24 cm
2

Personell, masks, cap,
overall cfu / 24 cm
2

100 < 3 3 < 1 < 1
10.000 < 20 5
10 (floor)
20 10
100.000 < 100 - - -


Test frequencies (air sampling) for environmental monitoring:

Acc. To USP chapter <1116> 2
nd
. Draft (March/April 1995)

Aseptic production (cleanroom area) Evaluation frequency
Class 100 Every shift
Class 10.000 Daily
Class 100.000 2 times per week
Class 100.000
(non-product / container contact)
1 time per week


AQUARIA MICROFLOW User Manual 12/08 rev.5 page 30



The Company

AQUARIA srl.
Via della Levata, 14 20084 Lacchiarella (MI) Italy

nella persona del suo Presidente e Legale rappresentante
in the person of President and Legal representative

Emilio Dadati


DICHIARA CHE
declares that

il campionatore daria microbiologico, mod. MICROFLOW
the MICROFLOW microbiological air sampler
Matricola N.

conforme alle seguenti normative Europee:
conforms to the following E.C. standard


Immunit: IEC 1000 4- 3 (R.F.E.M. Field)
Immunity: IEC 1000 4 2 (Electrostatic Discharge)

Emissione: EN 55011 (Conducted Emission)
Emission: EN 55011 (Radiated Emission Pre-Compl.)

Altre: CEI EN 50082 - 2 (Generic Immunity Std)
Others: CEI EN 50081 - 1 (Generic Emission Std)


Lacchiarella, .



AQUARIA srl.

The President


AQUARIA MICROFLOW User Manual 02/07 rev.1 page 31