You are on page 1of 3

Everjoy Kanyangarara

Core practical: Investigating the effect of Temperature on enzyme


activity

Hypothesis: temperature increases the rate of the enzyme-catalysed


reaction but at a certain temperature the enzymes are denatured and
temperature stops to have an effect on the reaction. This is a one-tailored
hypothesis.

Procedure: The equipment that were used in this experiment are: A


measuring cylinder, container, water baths-24°C,40°C,60°C,80°C and an
Ice bath, yeast cells, distilled water, scale, test tubes, gas delivery tube
connected to a bung, hydrogen peroxide, stop watch, pipette,

 Put water into the measuring cylinder and then place it face down in a
container full of water and then record the making that the water gets to.
 Measure out 0.1 grams of yeast cells on the scale and using a pipette
measure 2cm³ hydroxide into a test tube
 This is done under room temperature and at the time that I carried this
experiment out it was 24°C
 Connect the delivery tube to the measuring cylinder that is filled with
water
 Add the weighed yeast into the test tube containing the hydrogen
peroxide and quickly bung the test tube at the same time using a stop
watch record the amount of gas that is released within 30 seconds.
 Measure the new level at which the water is at and then take this value
from the original water level to know the amount of oxygen that is
produced
 Record the results into a table and then repeat the experiment twice with
the same temperature
 Repeat this experiment three time with all the other temperatures

Results:

Repeats Temperature

24°C 40°C 60°C 80°C Ice 1°C

Volume of O₂ Produced

1 30 21 20 9 10

2 32 22 21 10 11

3 34 25 23 13 12

Table 1:
Table for the results that I collected at the different
temperatures and the repeats that I carried out

Everjoy Kanyangarara
Everjoy Kanyangarara

Temperatu Volume of O₂ Rate of


res produced reactions

24°C 30 1

32 1.06

34 1.13

40°C 21 0.7

22 0.73

25 0.83

60°C 20 0.67

21 0.7

23 0.66

80°C 9 0.3

10 0.33

13 0.43

Ice 1°C 10 0.33

11 0.37

12 0.4

Table 2:
Table for the rates of reactions for all the different
temperatures used to carry out the experiment. Formula to work
out the rate of reaction= (Volume of O₂ produced) ÷ (time) this
was for 30 seconds. These are for all the repeats.

Temperatur Mean
es Rate of
reaction
s
1°C 0.37

24°C 0.91

Everjoy Kanyangarara
Everjoy Kanyangarara

40°C 0.75

60°C 0.68

80°C 0.35

This is the table that I used to sketch my graph as I found


Table 3:
the mean average rate of reactions for all the temperatures

Graph 1: this is the graph for the temperatures plotted against their rate of
reactions. The scale for the temperature =1-13. 1 represents 1 then from there
all the other values represent a number n*6. E.g. 4 is =24

Discussion: as the temperature increased the rate of the reaction


increased. I say this is so because at the start when the temperature was
1°C the rate of reaction was 0.37 and as the temperature got to 24°C the
rate of reaction increased to 0.91. As the reaction went on and the
temperature got to 40°C, the rate of reaction started to decrease. This
agreed with my hypothesis because for all the other temperatures the
rates of reactions decreased. This showed that there is a positive
correlation between the rate of reaction and the temperature. At 40°C
onwards the rate of reaction started to decrease, this agreed with my
hypothesis because the enzymes in the reaction became denatured
around 24°C -26°C so the reaction was not taking place at a fast rate
anymore. The rate at which kinetic energy was being produced was
decreasing because the temperature was decreasing so there was less
chance of the substrate and the active site colliding. So overall the rate of
reaction will decrease.

Everjoy Kanyangarara

You might also like