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14.03.08
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Four pattern of nuclear staining have been described:
1. Only peripheral or rim pattern (DNA pattern)
• Shows the most specific traditional ANA pattern for SLE
• Have two form that was differentiated by staining of chromosome
(chromosome pattern) or not
2. Homogenous pattern
• Specific for SLE
3. Speckled pattern (dot-dot)
• The least specific for SLE
• Also have two form that was differentiated by staining of
chromosome (chromosome pattern) or not
4. Nucleolar pattern (inside nucleus)
• Anti ribo-nucleoprotein (anti – RNP) in progressive systemic
sclerosis (scleroderma)
Anti – DNA:
1. Ab against ds DNA were more specific for SLE
2. Immunofluorescence test used hemoflagellate (Crithidia luciliae) as
substrate
• Kinetoblast contains ds DNA with no ss DNA
• Nucleus: both ss DNA and ds DNA (highly specific for SLE)
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Disease associated with anti – nuclear antibodies
1. MCTD: mix connective tissue disease
2. SLE: systemic lupus erythematosus
3. Sjorgen syndrome
4. Scleroderma
PSS (Scleroderma):
1. Replacement of smooth muscle throughout the body by fibrous
connective tissue
2. Nucleolar ANA (ribonucleoprotein) pattern was associated with PSS
Sjorgen syndrome:
1. Dry eyes, dry mouth, and arthritis (inflammation of the joints)
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