Professional Documents
Culture Documents
SRIKANTH
S/o Mr. N.Madhusudana Rao
Near R.T.C Workshop,
Bhavanipuram, 9966096407
Vijayawada 520 012 srikanth.nuka@gmail.com
Career objective
To have challenging and rewarding career where I can use my technical and
analytical skills.
Qualifications
Training undergone
Exposure to Methodology/Techniques/Instruments
1. INSTRUMENTATION: Agarose gel Electrophoresis, SDS-PAGE, Western
Blotting, RT- PCR, Gradient-PCR, Paper Chromatography.
2. TECHNIQUES: Isolation of genomic DNA, Isolation & Quantification of
RNA, Immunohistochemistry, Isolation & Estimation of Protein, Basic
Microbiological & Fermentation Techniques, Plant Tissue Culture Techniques
Seminars & Technical paper presentations
Educational Projects:
M.Sc. Project:
Title : Differential expression of CD34 and NOTCH in the normal and
cancerous tissues of gastric epithelium and its implication in carcinogenesis.
Location : University of Madras, Guindy campus.
Duration : June to August 2007
Guide : Dr.H.Devaraj
Description : Ectopic expression of CD 34 observed in gastric cancer not in normal
cells by RT-PCR indicates the presence of C 34 expressing undifferentiated HSC in
cancerous mass. NOTCH suppresses differentiation and promotes redifferentiation of
cancer stem cells, so its reexpression during cancer favours neoplastic transformation.
From experiment, it has been observed that differentiation of CD34 expressing HSC
cell precursors created by notch expression could promote tumour progression under
proliferative environment created by inflammation and their mediators IL-6 and IL-8.
B.Sc. Project:
Title : In vitro fruiting of Datura inoxia using Ovary Culture
Location : Andhra Loyola College, Acharya Nagarjuna University.
Duration : November 2004 to March 2005.
Guide : Fr.Dr.S.Emmanuel.sj.
Description : Evaluating the effect of plant growth hormones and effect of different
media on in vitro fruiting from ovaries. Experiments were conducted with MS
medium, Gamborg, B5 medium with different growth hormones like BAP, kinetin,
GA, NAA at different concentrations. Results obtained concluded that BAP is suitable
hormone to obtain in vitro fruiting on full strength MS medium.
Reference:
Declaration:
I hereby declare that the above written particulars are true and fair to the best
of my knowledge and belief.
NUKA.SRINIVASA SRIKANTH