Predicting the flowering cycle of Indian mustard plants through the glucosinolate analysis of the seed by near-infrared spectroscopy

Mercedes del Ro-Celestino*, Rafael Font and Antonio de Haro-Bailn Department of Agronomy and Plant Breeding !nstit"te of #"staina$le Agric"lt"re %C#!C& Alameda del '$ispo s(n )*+,+ Crdo$a #pain *e-mail- mdrc.cica es /l - 01* 234 *225))6 fa7- 01* 234 *22535 Abstract !ndian m"stard %Brassica juncea C8ern 9 Coss & is a plant species c:aracterised $y t:e presence of a :ig: concentration of er"cic acid and a large ;aria$ility in t:e gl"cosinolate pattern of t:e seed <:ereas B. juncea genotypes of ="ropean or >ort: American origin contain from )3+ to 5++ ?mol g -) of sinigrin %5-propenyl gl"cosinolate& %oil e7tracted, air-dried meal&, genotypes from t:e !ndian s"$continent contain ;aria$le amo"nts of sinigrin and gl"conapin %$"t-1-enyl gl"cosinolate& !n addition, ot:er minor gl"cosinolates are also present in t:e seed, s"c: as *-:ydro7ygl"co$rassicin /:e gl"cosinolate pattern e7:i$ited $y B. juncea seed ma@es it one of t:e most promising species as a potential so"rce of ;aria$ility for gl"cosinolates /:e Department of Agronomy and Plant Breeding %!nstit"te of #"staina$le Agric"lt"re, C#!C, Crdo$a, #pain& :olds a germplasm collection of B. juncea from ="rope and t:e !ndian s"$continent, s:oAing a large ;aria$ility in its oil and meal composition, incl"ding gl"cosinolates /:is germplasm collection :as $een t:e $asis for de;eloping near-infrared spectroscopy %>!R#& researc: in recent years %Font et al , 5++1& <e :a;e fo"nd t:ro"g: e7perience in field trials performed at !A#, Crdo$a, t:at t:e partic"lar pattern of gl"cosinolates fo"nd in t:e seed of t:is species, relates Ait: t:e floAering cycle of t:e plant According to t:is, genotypes containing mainly sinigrin s:oA a larger floAering cycle t:an t:ose :a;ing gl"conapin as t:e main gl"cosinolate /:is correlation alloAs "s to infer t:e floAering date from t:e gl"cosinolate pattern of t:e seed !n order to s"pport t:is fact, gl"cosinolate determinations :a;e to $e done t:ro"g: t:eir normal occ"rrence ranges, A:ic: implies t:e analysis of :"ndreds of seed samples to co;er t:e A:ole distri$"tion of t:ese compo"nds in t:e plant /:"s, t:e s"ccessf"l of t:e e7periment implies t:e "se of fast analytical tec:niB"es t:at alloA acc"rate res"lts !n a pre;io"s Aor@ %Font et al , 5++*&, Ae reported t:e "se of nearinfrared spectroscopy %>!R#& as a s"ita$le tec:niB"e for predicting indi;id"al and total gl"cosinolates in B. juncea seed /:e p"rpose of t:is Aor@ Aas to st"dy t:e relations:ip e7isting $etAeen floAering cycle and gl"cosinolate composition in t:e seed of B. juncea in field assays /o do t:is, >!R# Aas "sed as a rapid, c:eap and s"fficient acc"rate tec:niB"e for t:e analysis of gl"cosinolates and for predicting t:e floAering cycle in B. juncea References Font, R , M Del Ro, C M FernDnde8 9 A De Haro, 5++1 Acid detergent fi$er anDlisis in oilseed Brassicas $y near-infrared spectroscopy C Agri Food C:em 3)52)4-5255 Font, R , M Del Ro, C M FernDnde8-Martne8 9 A De Haro, 5++* Ese of nearinfrared spectroscopy for screening t:e indi;id"al and total gl"cosinolate content in !ndian m"stard seed %Brassica juncea F C8ern 9 Coss & C Agri Food C:em 3513G1-13G2