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Creating the calibration curve: Ferroin: Concentration (M) Absorbance 1.25E-05 0.100 2.50E-05 0.200 3.75E-05 0.320 5.00E-05 0.440 6.25E-05 0.450
2 pts
Place your calibration plot here. Make your plot big enough to cover this instruction box so that it is large Concentration of Ferroin vs. Absorbance enought for someone else to read. This calibration plot is Abs vs. concentration of ferroin (M) (y vs. x)
0.500 Use the online resources if you need help figuring out how plot a in Excel. y to = 7520x + graph 0.02 0.400 Title the graph and label the X and Y axis, including the correct units (Absorbance data is unitless). Be sure to double check your units and formatting once you print the report. Absorbance 0.300
Series1 Linear (Series1)
0.600
4 pts
Add a Trendline to show the linear fit of your data. Choose a linear line and choose the options that will 0.200 "display the equation on the chart".
0.100 0.000 0.00E+00
1.00E-05
2.00E-05
3.00E-05
4.00E-05
5.00E-05
6.00E-05
7.00E-05
Slope of Absorbance versus concentration graph -1 7520 M y-intercept of Absorbance versus concentration graph 0.02 Detailed calibration equation: y = 7520x + .02 Determining theAmount of Iron in an Iron Tablet 1) 2) 3) Average mass of a tablet Mass of crushed tablet used in analysis Final volume after filtered crushed tablet solution is diluted in volumetric flask (lab manual Part II, Step 5) Volume of diluted crushed tablet solution transferred to the new volumetric flask Final volume of ferroin complex solution (end of lab manual Part II, Step 9) Absorbance of the ferroin complex solution (lab manual Part III, Step 3) 436 19 100
1 pt 1 pt 2pts
mg mg mL 1 pt
(provided in manual)
4)
mL
5)
100
mL
6)
0.130
1 pt
7)
Using the calibration equation and the absorbance you measured for the prepared sample, calculate the ferroin concentration. Show your work and don't forget to include units. .13 = 7520x + .02 x = 1.46E-5 M
3 pts
8)
Based on the procedural steps and the ferroin concentration you just calculated, calculate the moles of ferroin in the final ferroin complex solution prepared in Part II, Step 9. Show your work. 1.46E-5 M / 1000 mL/L x 100 mL = 1.46E-6 mol
2 pts
9)
Based on the moles of ferroin in the final ferroin complex solution, calculate the moles of iron in the crushed tablet solution prepared in Part II, Steps 2-5. Show your work. 1.46E-6 mol x (100/5) = 2.92E-5 mol
3 pts
10)
Using the "moles of iron in the crushed tablet solution" you just calculated, calculate the mass (in mg) of iron in the crushed sample that you weighed out. Show your work. 2.92E-5 mol x 55.85 g/mol x 1000 mg/g = 1.63 mg
2 pts
11)
From the mass of iron in the crushed tablet sample you weighed out, calculate the mass (in mg) of iron in a whole tablet. Show your work. 1.63 mg = x 20 mg 436 mg x = 35.5 mg
3 pts
12)
27
mg
2. If you did not wait for the complete formation of the ferroin complex in Part II, step 10, how would your data look different and how would this affect your determination of the mass of iron in the tablet? ( 5 pts) If the feroin complex had not fully formed, the absorbancy would have been affected and the resultant mass of iron found in the tablet would have been too little. 3. You use atomic emission spectroscopy, another spectroscopic technique, to measure the Li+ concentration in 5 standard solutions of varying concentrations of LiCl. The intensities for the standard solutions are plotted versus the concentrations and the resulting calibration equation is: Intensity = 84,250 M-1 * [Li+] + 1.75
If the intensity of your unknown sample is 90, what is the concentration of Li+ in the analyzed sample? (5 pts)
[Li+] = 1.05E-3 M
If 2 mL of the original unknown sample was diluted to 150 mL prior to analysis, what is the concentration of Li+ in the original solution? (5 pts) .001 M x .001 L/mL x 2 mL / 150 mL = 1.33E-8 M
Laboratory Waste Evaluation (1 pt) Laboratory waste is considered anything generated during an experiment that is disposed of down the sewer drain, thrown in the garbage, collected in a container for disposal by the UW Environmental Health & Safety department, or released into the environment. Based on the written lab procedure and your actions during the lab, list the identity and approximate amount (mass or volume) of waste that you generated while performing this experiment.
- 2 mL 1 M HCl - .019 g iron - 2 mL hydroxylamine hydrochloride .23 M - 1 mL 1 M Na acetate sol. - 3 mL 3.6E-3 phenanthroline - around 250 mL water - about 400 mg of miscellaneous contents of iron tablet - wrappers from new lab equiptment - a few tissues