Blood Sampling

Regen PRP PRP PRP

Draw 8 cc of blood

Draw 13.5 cc ( 15cc Kit ) of blood 18 cc ( 20cc Kit ) of blood 27 cc (30cc Kit ) of blood

By sampling the 18cc blood PRO PRP KIT can extract the needed PRP volume but Regen kit have to use 2 tubes to extract PRP.

Move Drawn Blood into the PRP KIT

Regen PRP PRO PRP

Regen kit, containing Gel, is a kind of vacuum blood collection tube, does not move blood into another container.

Move drawn blood into the PRP kit.

Gel material injected into human body may possibly cause allergy, foreign body reactions, and blockages in blood vessels.

Centrifugation
Regen PRP PRO PRP

centrifugation Speed : 1500G (3100 ~3400 RPM) Time : 9~15 min

Centrifugation Speed : 1050G (2800 RPM) Time : 5 min

Regenlab recommends centrifugation at 3400rpm/5min, it may be a condition to cause more damages. PRO PRP Centrifuge start slowly and stop slowly In order to get fine PRP without damages

After complete Concentration

Regen PRP PRO PRP

Regen kit, containing gel in tube, separates serum and RBC layer like the blood collection tube.

After centrifugation, PRO PRP separates PPP/PRP/Buffy Coat/RBC layer, and you can watch the movement of Buffy coat layer with the naked eye with parallel Layer which can extract fine PRP

*Regen kit: Each layer is not separated completely, so you can find some RBCs in gel, which means Buffy coat layer is not completely separated and some remains in gel and RBC layer, causing loss of platelets. Slope layer dont make to Extract fine PRP

Analysis
Regen PRP PRO PRP

A A

Regen have insisted that A is PRP but A is not a concentrated platelet but Serum . Serums quantity is about 3.5~4cc . If insist all of 3.5~4cc PRP , its PRP density might be low. If saying Regens insist for our Kit, PRO PRP Kit can say to get 10~12cc (20cc Kit ) PRP including BuffyCoat on the aspect of Regen . But PRO PRP is 2cc high density PRP including BuffyCoat . If you want to increase qty of PRP , you can extract more PRP by including PPP Layer more.

Reference

Reference

Extraction Step
Regen PRP PRO PRP

Withdraw 0.9cc ~ 4cc

Extraction of PRO PPP Minimize PRP loss by complete PRP extraction.

Regen kit uses the whole plasma layer after centrifugation, without separation process of PPP/PRP/Buffy coat layers, so the concentration of platelets is low and less effective in treatment. And, the extraction using a needle may possibly cause contamination.

Summary
Regen PRP PRO PRP

*PRO PRP Kit may adjust the concentration of PRP, by adjusting the volume of PPP, and also adjust RBC inclusion volume and Buffy-coat layer at doctors opinion and discretion.

Microscope Images of the extracted PRP

A and B show the images of platelets clustered and activated after centrifugation. (These images are not the case of Regen PRP). In these conditions, PRP has no proper effects after the PRP procedure. -Growth Factors already released outside the body are less effective in the body. This phenomenon is found in the case of high-speed centrifugation, long time or repeated centrifugations. * C is the image of platelets extracted by PRO PRP system, which shows each platelet in unimpaired, stable(inactivated) conditions.

PRP PRP Microscope images

Stable state

PRO PRP SYSTEM

For effective PRP treatment, platelets should be kept In their original conditions. (they should not be activated in the early stages, but collected in unimpaired conditions). Activated state

Conclusion
Regen
Centrifugation condition Possibility of contamination while extracting Gel Dangerousness of Gel 3100 RPM/9min or 3400RPM/5min Possible contamination by using a needle Used Gel material injected into human body may possibly cause allergy, foreign body reactions, and blockages in blood vessels. 8cc Difficult Difficult to get PRP and PPP separately : PRP + PPP = about 4cc Difficult (Fixed) Difficult (Fixed)

PRO PRO
3,300RPM/ 5min (15cc,20cc) 2,800RPM/ 5 min (30cc) Little possibility by not using a needle Not used Just use the difference of density gradient (among, RBC, buffy-coat and plasma) without Gel Blood 16.5cc + anticoagulant 3.5cc ( total 20cc) Easy to control Extract PRP and PPP separately PRP + PPP = about 11cc Easy Easy

Volume Control of PRP concentration Extraction volume of PPP, PRP Extraction of highconcentrated PRP Extraction of lowconcentrated PRP

PRO PRP KIT TEST RESULT

3.000.000 2.500.000 2.000.000 1.500.000 1.000.000 500.000 A B C Base line D Test 1 E Test 2 F Test 3 G H I J Average Concentration rate(Max)

(Tested by Green Cross Reference Lab)

1009% 773% 672% 806% 696% 809% 708% 608%

1100% 1000% 900%

733%

792%800%
700% 600% 500% 400% 300% 200% 100% 0%

Patient Name
A B C D E F G H I J Average

Base line
214,000 215,000 283,000 283,000 304,000 200,000 258,000 193,000 300,000 143,000 239,300

Test 1
1,366,000 1,397,000 1,815,000 2,267,000 2,113,000 1,666,000 1,826,000 1,324,000 1,824,000 1,231,000 1,682,900

Test 2
1,439,000 1,662,000 1,970,000 2,280,000 2,229,000 2,202,000 1,630,000 1,290,000 1,288,000 1,254,000 1,724,400

Test 3
1,424,000 1,462,000 1,721,000 2,137,000 1,925,000 1,925,000 1,622,000 1,561,000 1,439,000 1,443,000 1,665,900

Concentration rate (Max)

672% 773% 696% 806% 733% 1101% 708% 809% 608% 1009% 792%

TEST REPORT

Patient A

TEST REPORT

Patient B

Comparison between PRO PRP KIT & other PRP KITS

(Tested by Green Cross Reference Lab)
1101% 2.202.000 3.000.000 2.500.000 2.000.000 1.500.000 274.000 266.000 247.000 200.000 247.000 1.000.000 500.000 0 YES PRP A Base line B PRP count C Concetration rate D E 1200% 1.899.999 1.899.000 1.907.000 1.776.000 1000% 800% 600% 400% 200% 0%

769%

769%

696%

668%

590%

Company Name
YES PRP
A B C D E

Base line
200,000
247,000 274,000 266,000 258,000 258,000

PRP count
2,202,000
1,899,000 1,907,000 1,776,000 1,522,000 1,150,000

Concentration rate
1101%
769% 696% 668% 590% 446%

258.000

1.522.000

Microscope Verification Result

Old

young

Certificate Of numbers of platelets using YES PRP kit Examined by laboratory at Cell & Tech corp.