Biotechnology: Introduction to Gel Electrophoresis Go to: http://www.classzone.com/cz/books/bio_07/resources/htmls/virtual_labs/virtualLabs.

html Name: Selena Feli Explore: Define the following/Describe what is it used for: * !"arose Gel: ! "el that hol#s $N! samples an# its use# %or the seperation o% the $N! %ra"ments. & $N! 'arkers: $N! %ra"ments with known len"ths. & $i"este# $N! Samples: $N! samples that have alrea#( been cut b( the restriction enz(mes. & )lectrophoresis *hamber: +here the process o% electrophoresis takes place, where the ne"ative char"e $N! travel to the positive si#e o% the chamber. & -ower Suppl(: .t is use# to pro#uce electric currents in the electrophoresis chamber. & /romophenol /lue: it is a #(e that #(es the $N! movements so we can track the #istance o% the $N!. & 0/) /u%%er: .t is use# to help carr( electric current in the "el. & Gel Stainin" 0ra(: 1ol#s the a"arose "el a%ter bein" separate. & )thi#ium /romi#e: 0he %luorescent #(e use# %or stainin" $N!. & 'icropipette: .s use# to trans%er #(e an# $N! samples. & -ipette 0ips: 0heir use# %or the trans%er o% #(e or $N! samples an# uses a clean tip %or ever( #i%%erent solution to avoi# contamination. & $eionize# +ater: 2se# to rise the "el a%ter it has been staine# with the ethi#ium bromi#e. & 23 Li"ht /o : 2se# to help rea# the $N! marks on the "el that has been staine# b( the ethi#ium bromi#e Procedure: Step #1: Explain how comparing DNA Fingerprints can help identify a person who has committed a crime *omparin" $N! %in"erprints can help i#enti%( a person who has committe# a crime because ever(one has #i%%erent %in"erprints an# i% the( %in# the %in"erprint at the crime scene then that person can be a suspect an# coul# have potentiall( committe# the crime.

Go through the steps #2-11 Record the BASICS o% each step below: Step 4: -ut the 0/) bu%%er solution onto the a"arose "el to cover the sur%ace o% it. Step5: +e pipette bromophenol blue #(e an# mi it with the marker $N! an# then e6ect it into the marker $N! well. Step 7: -ipette the blue #(e into the victims $N! an# mi it. 0hen pipette it in the victims well. Step 8: -ipette the blue #(e into victim 9:s $N! an# mi it, then loa# it onto victim 9:s well. Step ;: -ipette the blue #(e into victim 4:s $N! an# mi it, then loa# it onto victim 4:s well. Step 7: -ipette the blue #(e into the evi#ence $N! an# mi it, then loa# it into the evi#ence well. Step <: *onnect the electo#es %rom the power source to the eletophoresis chamber to create electric currents %or one hour. Step =: 0rans%er the ara"arose "el to the stainin" tra( an# %loo# the "el with ethi#ium bromi#e. Step #12: Questions 9: Ev lu te: Does the DNA found on the hair sample match suspect #1 or suspect #2 !ow do you "now Suspect #1 4: An ly!e: #hy do a series of $ands appear on the gel They are the Suspects DNA, Victims DNA, Ladder (used for comparisons), DNA form cigarette butt, and DNA from fingernail clippings. All these are used to compare and see if they have a match with the DNAs. 5: Identi"y C use: #hy is the largest DNA fragment $and found closest to the well in which it was placed The further you go down, the smaller they are. 7: In"er: #hat is true of the DNA fragment $and closest to the positi%e end of the gel It is the smallest. 8: Predict: #hat would happen if the electrodes were plugged into the wrong outlets

The DNA would be incorrect. ;: Apply: #hy is DNA fingerprinting more conclusi%e when pro%ing a person&s innocence rather than their guilt The DNA does not match up so they are proven innocent if it was all up to the DNA results.