Exam Name___________________________________

MULTIPLECHOICE.Choosetheonealternativethatbestcompletesthestatementoranswersthequestion. 1) TheimmediateenergysourcethatdrivesATPsynthesisbyATPsynthaseduringoxidative phosphorylationisthe A) H+ concentrationacrossthemembraneholdingATPsynthase. B) oxidationofglucoseandotherorganiccompounds. C) flowofelectronsdowntheelectrontransportchain. D) affinityofoxygenforelectrons. E) transferofphosphatetoADP. 2) Whenskeletalmusclecellsareoxygen- deprived,theheartstillpumps.Whatmusttheheart musclecellsbeabletodo? A) removelactatefromtheblood B) continueaerobicmetabolismwhenskeletalmusclecannot C) derivesufficientenergyfromfermentation D) transformlactatetopyruvateagain E) removeoxygenfromlactate 2) 1)

Exposinginnermitochondrialmembranestoultrasonicvibrationswilldisruptthemembranes.However,thefragmentswill resealinsideout.TheselittlevesiclesthatresultcanstilltransferelectronsfromNADHtooxygenandsynthesizeATP.If themembranesareagitatedfurther,however,theabilitytosynthesizeATPislost. 3) Theseinside - outmembranevesicles A) willreverseelectronflowtogenerateNADHfromNAD + intheabsenceofoxygen. B) willbecomeacidicinsidethevesicleswhenNADHisadded. C) willhydrolyzeATPtopumpprotonsoutoftheinteriorofthevesicletotheexterior. D) willbecomealkalineinsidethevesicleswhenNADHisadded. E) willmakeATPfromADPand i iftransferredtoapH4bufferedsolutionafterincubation inapH7bufferedsolution. 4) ApproximatelyhowmanymoleculesofATPareproducedfromthecompleteoxidationoftwo moleculesofglucose(C6 H12O6 )inaerobiccellularrespiration? A) 6064 B) 2 C) 15 D) 4 E) 3032 5) 4) 3)

5) Howmanycarbonatomsarefedintothecitricacidcycleasaresultoftheoxidationofone moleculeofpyruvate? A) six B) two C) eight D) ten E) four 6) Wherearetheproteinsoftheelectrontransportchainlocated? A) mitochondrialmatrix B) mitochondrialintermembranespace C) mitochondrialoutermembrane D) mitochondrialinnermembrane E) cytosol

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7) InadditiontoATP,whataretheendproductsofglycolysis? A) CO 2 andNADH B) CO 2 andH 2 O C) CO 2 andpyruvate D) NADHandpyruvate E) H2 O,FADH2 ,andcitrate 8) Invertebrateanimals,brownfattissuescolorisduetoabundantbloodvesselsandcapillaries. Whitefattissue,ontheotherhand,isspecializedforfatstorageandcontainsrelativelyfewblood vesselsorcapillaries.Brownfatcellshaveaspecializedproteinthatdissipatestheproton - motive forceacrossthemitochondrialmembranes.Whichofthefollowingmightbethefunctionofthe brownfattissue? A) toregulatetemperaturebyconvertingmostoftheenergyfromNADHoxidationtoheat B) toallowtheanimalstoregulatetheirmetabolicratewhenitisespeciallyhot C) toincreasetherateofoxidativephosphorylationfromitsfewmitochondria D) toallowothermembranesofthecelltoperformmitochondrialfunctions E) toincreasetheproductionofATP 9) Ifacellisabletosynthesize30ATPmoleculesforeachmoleculeofglucosecompletelyoxidizedby carbondioxideandwater,howmanyATPmoleculescanthecellsynthesizeforeachmoleculeof pyruvateoxidizedtocarbondioxideandwater? A) 1 B) 15 C) 12 D) 14 E) 0 10) ThedirectenergysourcethatdrivesATPsynthesisduringrespiratoryoxidativephosphorylation ineukaryoticcellsis A) oxidationofglucosetoCO 2 andwater. B) thethermodynamicallyfavorableflowofelectronsfromNADHtothemitochondrialelectron transportcarriers. C) thethermodynamicallyfavorabletransferofphosphatefromglycolysisandthecitricacid cycleintermediatemoleculesofADP. D) thefinaltransferofelectronstooxygen. E) theproton- motiveforceacrosstheinnermitochondrialmembrane. 11) WhichofthefollowingproducesthemostATPwhenglucose(C 6 H12O6 )iscompletelyoxidizedto carbondioxide(CO2 )andwater? A) oxidationofpyruvatetoacetylCoA B) glycolysis C) oxidativephosphorylation(chemiosmosis) D) citricacidcycle E) fermentation 12) Whichkindofmetabolicpoisonwouldmostdirectlyinterferewithglycolysis? A) anagentthatreactswithoxygenanddepletesitsconcentrationinthecell B) anagentthatblocksthepassageofelectronsalongtheelectrontransportchain C) anagentthatcloselymimicsthestructureofglucosebutisnotmetabolized D) anagentthatreactswithNADHandoxidizesittoNAD + E) anagentthatbindstopyruvateandinactivatesit

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13) Startingwithonemoleculeofglucose,theenergy - containingproductsofglycolysisare A) 2NAD+ ,2pyruvate,and2ATP. B) 6CO2 ,30ATP,and2pyruvate. C) 2NADH,2pyruvate,and2ATP. D) 2FADH2 ,2pyruvate,and4ATP. E) 6CO2 ,2ATP,and2pyruvate. 14) Whyisglycolysisconsideredtobeoneofthefirstmetabolicpathwaystohaveevolved? A) Itisfoundinprokaryoticcellsbutnotineukaryoticcells. B) Itrequiresthepresenceofmembrane- enclosedcellorganellesfoundonlyineukaryoticcells. C) Itreliesonchemiosmosis,whichisametabolicmechanismpresentonlyinthefirstcells prokaryoticcells. D) Itdoesnotinvolveorganellesorspecializedstructures,doesnotrequireoxygen,andis presentinmostorganisms. E) ItproducesmuchlessATPthandoesoxidativephosphorylation. 15) Duringaerobicrespiration,H2 Oisformed.Wheredoestheoxygenatomfortheformationofthe watercomefrom? A) pyruvate(C3 H3 O3 - ) B) glucose(C6 H12O6 ) C) carbondioxide(CO2 ) D) lactate(C3 H5 O3 - ) E) molecularoxygen(O2 ) 16) Youhaveafriendwholost7kg(about15pounds)offatonaregimenofstrictdietandexercise. Howdidthefatleaveherbody? A) Itwasconvertedtoheatandthenreleased. B) Itwasbrokendowntoaminoacidsandeliminatedfromthebody. C) ItwasconvertedtoATP,whichweighsmuchlessthanfat. D) ItwasreleasedasCO 2 andH 2 O. E) Itwasconvertedtourineandeliminatedfromthebody.

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Figure9.2Thecitricacidcycle. 17) Startingwithcitrate,whichofthefollowingcombinationsofproductswouldresultfromthree acetylCoAmoleculesenteringthecitricacidcycle(seeFigure9.2)? A) 3ATP,3CO 2 ,3NADH,and3FADH 2 B) 3ATP,6CO 2 ,9NADH,and3FADH 2 C) 2ATP,2CO 2 ,3NADH,and3FADH 2 D) 38ATP,6CO2 ,3NADH,and12FADH 2 E) 1ATP,2CO 2 ,3NADH,and1FADH 2 18) Whichprocessineukaryoticcellswillproceednormallywhetheroxygen(O 2 )ispresentorabsent? A) oxidativephosphorylation B) electrontransport C) thecitricacidcycle D) chemiosmosis E) glycolysis 19) Whatisthepurposeofbetaoxidationinrespiration? A) controlofATPaccumulation B) feedbackregulation C) oxidationofpyruvate D) oxidationofglucose E) breakdownoffattyacids 19) 18) 17)

Figure9.1illustratessomeofthesteps(reactions)ofglycolysisintheirpropersequence.Eachstepislettered.Usethese letterstoanswerthequestions.

Figure9.1 20) WhichportionofthepathwayinFigure9.1containsaphosphorylationreactioninwhichATPis thephosphatesource? A) A B) B C) C D) D E) E 20)

Figure9.3 21) WhathappensattheendofthechaininFigure9.3? A) 2electronscombinewithamoleculeofoxygenandtwohydrogenatoms. B) 1electroncombineswithamoleculeofoxygenandahydrogenatom. C) 4electronscombinewithfourhydrogenandtwooxygenatoms. D) 2electronscombinewithaprotonandamoleculeofNAD + . E) 4electronscombinewithamoleculeofoxygenand4protons. 22) Theoxygenconsumedduringcellularrespirationisinvolveddirectlyinwhichprocessorevent? A) thephosphorylationofADPtoformATP B) glycolysis C) acceptingelectronsattheendoftheelectrontransportchain D) theoxidationofpyruvatetoacetylCoA E) thecitricacidcycle 22) 21)

23) Ayounganimalhasneverhadmuchenergy.Heisbroughttoaveterinarianforhelpandissentto theanimalhospitalforsometests.Theretheydiscoverhismitochondriacanuseonlyfattyacids andaminoacidsforrespiration,andhiscellsproducemorelactatethannormal.Ofthefollowing, whichisthebestexplanationofhiscondition? A) Hiscellslacktheenzymeinglycolysisthatformspyruvate. B) HiscellscannotmoveNADHfromglycolysisintothemitochondria. C) Hiscellshaveadefectiveelectrontransportchain,soglucosegoestolactateinsteadofto acetylCoA. D) Hismitochondrialackthetransportproteinthatmovespyruvateacrosstheouter mitochondrialmembrane. E) Hiscellscontainsomethingthatinhibitsoxygenuseinhismitochondria. 24) Duringaerobicrespiration,whichofthefollowingdirectlydonateselectronstotheelectron transportchainatthelowestenergylevel? A) NADH B) FADH2 C) ATP D) ADP+ i E) NAD+

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25) Insideanactivemitochondrion,mostelectronsfollowwhichpathway? A) citricacidcycleFADH 2 electrontransportchain ATP B) electrontransportchain citricacidcycle ATP oxygen C) glycolysisNADHoxidativephosphorylation ATP oxygen D) citricacidcycleNADHelectrontransportchain oxygen E) pyruvate citricacidcycleATP NADH oxygen

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AnswerKey Testname:CH9

1) A 2) B 3) B 4) A 5) B 6) D 7) D 8) A 9) C 10) E 11) C 12) C 13) C 14) D 15) E 16) D 17) B 18) E 19) E 20) A 21) E 22) C 23) D 24) B 25) D