Progress Report 2011 Biofilms

BIOFILMS RESEARCH CENTER FOR BIOINTERFACES
A translation research programme at Malm University In partnership with the Knowledge Foundation Progress report #7
JANUARY 1, 2011 - DECEMBER 31, 2011
Biofilms - Research Center for Biointerfaces
Progress Report 2011
CENTER MISSION STATEMENT

Biofilms Research Center for Biointerfaces is a translational research programme covering five research groups within three faculties/schools at Malm University. The core strengths of the Center is i) our broad expertise, spanning the range from theoretical modeling to clinical sciences, and ii) our long experience in working with industry and relating to their needs. The general aim of the research activities at the Center is to understand, predict and control material/cell/tissue interactions with medical, dental, food and environmental applications. We strive to further integrate education (BSc, MSC, PhD), a cornerstone for the future of the Center. Particularly, one goal is to further integrate Master-level education into our research activities also at an operational level in specific projects. Biofilms Research Center for Biointerfaces has a goal to become a regional venue for a creative environment of biomedical technology in the resund region, facilitating collaboration between academic research, higher education and industry, based on pharmaceutical technology, biotechnology and medical technology, which together constitutes a key area of significant commercial growth potential. We strive to become a prime entry point for regional industry seeking translational academic expertise in the Biomedical technology field.
CONTENT
DIRECTORS REPORT 04
LIST OF CENTER MEMBERS
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COLLABORATIVE PARTNERS OF THE CENTER
LIST OF RESEARCH ACTIVITIES
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RESEARCH HIGHLIGHTS
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IST OF PUBLICATION L AT THE CENTER FROM 2011 AND ONWARDS
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SCIENTIFIC INSTRUMENTATION AND EQUIPMENT
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VISITORS AND SEMINARS AT THE CENTER
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WORKSHOPS AND CONFERENCES ORGANISED BY THE CENTER
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ONTRIBUTIONS TO AND C PARTICIPATION IN CONFERENCES AND WORKSHOPS
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HD THESES SUPERVISED P BY CENTER MEMBERS
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BSc AND MSc EDUCATION
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THER ACTIVITIES O BY CENTER MEMBERS
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ABBREVIATIONS
Mah: Malm University; LU: Lund University; KTH: The Royal Institute of Technology; LuTH: Lule University of Technology; UU: Uppsala University; LiU: Linkping University; KI: Karolinska Institute; GU: Gothenburg University; OD: Faculty of Odontology; HS: Faculty of Health and Society; TS: School of Technology. KKs: The Knowledge Foundation; VR: The Swedish Research Council; EU: European Union; FP6: 6th Framework Programme; FP7: 7th Framework Programme; SI: Swedish Institute; BMT: Biomedical Technology BMMT: Biomedical Methods and Technology; MS: Materials Science; FLK: The Research School in Pharmaceutical Science; BMA: Biomedical Laboratory Science; TELMah: Technology, Economy and Leadership at Malm University 3
THE DIRECTORS REPORT

2011 has been a year with many changes for Biofilms Research Center for Biointerfaces. The research profile grant from the Knowledge Foundation came to an end in February and in conjunction with this the management structure was changed and a new Steering Group and a new Director were appointed. Summing up the year, it can be concluded that the Center has managed the transformation into an independent Center and has succeeded in becoming stronger. The Center has extended its industry network, increased the number of researchers, and handled 35 individual research projects. In addition, Center members have been granted more research funding during 2011 than in any year in the past. The Knowledge Foundation granted the Center funding for seven new projects during 2011 and remains an important source of financial support. The Knowledge Foundation has formally evaluated the research profile investment during 2011 and in the evaluation it was concluded that research at Biofilms Research Center for Biointerfaces demonstrates a high level of quality. An internationally competitive environment has been built up in a field that is highly relevant to industry. This has contributed to the Universitys strategic development in research and education, as well as corporate development. Lory Melin Svanborg, Jildiz Hamit Eminovski, and Maria Pihl success4
fully defended their PhD theses in 2011. Julia Davies, Hkan Eriksson and Lennart Ljunggren were appointed as Professor and Sergey Shleev was appointed as Associate Professor. Several new members, Per Jnsson, Alexei Iantchenko, and Yuanji Cheng, have been affiliated to the Center. The Center also acquired a new Director on March 28, 2011, when Johan Engblom handed over to Anna Holmberg. Drs Olga Santos, Laura Varas, Jovice Bon Singh, Ida Svendsen and Luis Chavez de Paz left their positions as researchers during the year, while three new postdocs and one PhD student were recruited. Several senior researchers paid visits to universities outside Sweden, including Japan, Russia, Germany and Norway. The management structure of the Center has been improved during 2011. The Steering Group and the Reference Group have been merged into one board and the Executive Group has been replaced by a Senior Researchers Forum (SRF). SRF is a forum for sharing knowledge between different disciplines involved in the Center and one of the main roles of SRF is to identify strategically important research activities and to work together to attract funding for prospective projects. A new Steering Group was appointed by the Vice-Chancellor for the period July 1, 2011June 31, 2013. During 2011, we produced 70 publications in international journals as well as ten book chapters and three publica-
tions in the popular press. Publications appeared in journals such as Arthritis and Rheumatism (2010 impact factor, 7.3), Biosensors and Bioelectronics (5.4), Acta Biomaterialia (4.8), Bone (4.6), Journal of Physical Chemistry C (4.5), Langmuir (4.3), Electrochemistry Communications (4.3), Electrochimica Acta (3.6), Journal of Physical Chemistry B (3.6) and Biofouling (3.3). The high impact factors reflect the substantial scientific quality of the research carried out at the Center. In addition, 45 oral and 32 poster presentations were made at national and international meetings. A review of the number of citations over the past five years from papers produced by the Center members revealed 8,828 for permanent staff and 1,193 for junior researchers and post-docs. Five members had more than 500 citations each for the period 2007-2011, Ann Wennerberg, Tautgirdas Ruzgas, Gunilla Nordin Fredrikson, Thomas Arnebrant, and Sergey Schleev. Center members also refereed papers for international journals on a regular basis. Members of the Center take part in the National Graduate Research School in Odontology (Ann Wennerberg, co-ordinator, Gunnel Svenster, local co-ordinator and Julia Davies, supervisor). Most researchers participate in undergraduate (BMA and TELMah programmes) and/or graduate teaching (BMMT, MS and Dentistry programmes), as well as PhD student supervision. We strive to further integrate education (BSc, MSc, PhD), a
cornerstone in the future of the Center. One goal in particular is to integrate Masters level education even further into our research activities on the operational level in specific projects. Research collaboration with industry takes place in all four focal areas of the Center. During 2011, we started new research projects with Arla Foods AB, Bioglan AB, Nares AB, Eka Chemicals AB, Magle AB, Layer Lab AB, Galenica AB, Novosense AB, Ospol AB, Nobel Biocare AB, and Promimic AB. Although the borders between the focal areas are not always clear cut, the company involvement is as follows: i) Eucaryotic cell-surface interactions (Ospol, Promimic), ii) Molecular transport phenomena (AkzoNobel, YKI, ACO Hud Nordic, Eviderm Institute, Nares, Bioglan, Q-Med), iii) Molecular interactions at biointerfaces (Anordica, Acram, Bioglan) and iv) Microbial biofilms (Arla Foods, Acram, Biogaia, Nobel Biocare). We also have close collaboration with Medeon AB and Medicon Valley Alliance. Center members are partners in the collaborative EU FP7-funded project Three-dimensional nanobiostructure-based, self-contained devices for biomedical application (with Sergey Shleev as co-ordinator) and in the EU FP7 Marie Curie Initial Training Network MagnIM. We are also involved in three EU Interreg programmes; Medicon FISC, Valorisation of knowledge intensive ideas in the South Baltic area
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(SB-VALOR), and resund materials innovation community (-MIC). Center members have been responsible for arranging two workshops, NonInvasive Biomedical Monitoring and Devices (Biofilms 7th Annual Workshop), which attracted 100 participants from different universities and industries, and an Inspiration Day, an interdisciplinary workshop with 50 participants from industry, as well as partners and Center members. A
Malm, 29 February 2012 Anna Holmberg Director Biofilms Research Center for Biointerfaces
symposium Hydration of nanostructured and biological materials, which brought together 30 researchers from Malm University, Vilnius University, St. Petersburg State University and Lund University, was also organised as well as a partner meeting for the project Humectants and their mechanisms in skin. Center activities have also been highlighted in other ways, including an article in Dagens Industri and Rapidus Newsletter as well as two press releases and Malm University newsletters.
BIOFILMS RECEIVES MAJOR GRANT

The Knowledge Foundation has awarded research grants for a number of projects that will be run with partners in industry. Biofilms Research Centre for Biointerfaces will receive SEK 10 million for three research projects. Anna Holmberg, Director of Biofilms Research Center for Biointerfaces, is delighted: This is a great boost. The three research projects that have received funding all fall within our core strengths. The grants mean that we can continue to reinforce and intensify our research. The projects, which deal with oral implants, tools for non-invasive tests and the interaction of polysaccharides with water, involve collaboration with seven different companies, three of which are completely new partners. Anna Holmberg continues: There has been a steady increase in the number of partners in recent years. At present, we collaborate with 40 different companies and there is growing interest from industry. The research project Devices for
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non-invasive biomedical sensing, monitoring and/or delivery aims in time to offer patients test procedures that cannot be felt. Together with his research colleagues at Health and Society, Professor Tautgirdas Ruzgas will develop a biosensor that will enable different tests to be carried out without troubling the patient. Among other things, the aim is for the biosensors to measure neurotransmitters, such as dopamine, and different hormones. The measurement results from these substances can be used as indicators for cancer, Parkinsons disease, depression and other conditions. It is difficult to make these measurements at present without troubling the patient. In time, however, researchers hope to be able to offer a more patient-friendly alternative, such as a patch. Professor Ruzgas explains: In our research project, we will attempt to compensate for individual skin features to allow the patch to be used by everyone, regardless of age or skin type. Under the leadership of Senior Lecturer Vitaly Kocherbitov, a research group at Health and Society will study the properties of polysaccharides when they interact with water. Vitaly Kocherbitov states that polysaccharides, a sugar group, are degradable. This is a significant advantage when used as a packaging material or a drug carrier. In the research project Carbohydrate polymer - water interactions: sorption, porosity and rheology we will collaborate with industry in these specific areas of use.
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The manner in which the properties of polysaccharides change when they come into contact with water is central a packaging material, for example, needs to be stable when the humidity changes. In the same way, it must be possible to attach an active substance to a drug carrier outside the body although once it enters the body the substance must be released and the drug carrier needs to degrade. Vitaly Kocherbitov continues: We can help industry to understand what happens and prepare polysaccharides in such a way that they function in practice. This could be of major significance in a number of areas, the green packaging industry being a good example. The research project Oral implants Nanostructures for the promotion of tissue integration and prevention of infections aims to develop better oral implants, thus reducing the risk of infection in the long term. The project is headed by Professor Ann Wennerberg and Professor Gunnel Svenster at the Faculty of Odontology. This is an extremely exciting collaboration project which, apart from working with industry, further enhances the close co-operation that already exists between the research groups. Oral Biology has the expertise to carry out microbial analyses and at the Department of Prosthetic Dentistry we know about surfaces and tissue integration, states Professor Wennerberg. Implants need to satisfy two requirements: tissue integration, which means
good contact with the cells in the body, and assurance that there is no bacterial adherence to cells, thus avoiding infection. The hypothesis is that the surface of the material will direct bacterial activity. Were developing methods for examining bacterial activity and identifying any activity the bacteria have in common that could be instrumental in the development of infection.
Last year, Biofilms secured more than SEK 20 million in research grants. Things are looking very bright. We have a whole range of activities and by international comparison research within our core strengths is very solid indeed, concludes Professor Wennerberg.
Malm University Newsletter, 2012-02-23
LIST OF CENTER MEMBERS

During the reporting period, the Center comprised the following members (permanent staff, post-docs, PhD students and technical and administrative staff).
1. PERMANENT STAFF
Anna Holmberg Dr., Director,2011 Johan Engblom Assc. Prof., Director 2008-2011 Thomas Arnebrant Prof., Director 2005-2007 Tautgirdas Ruzgas Prof. Gunilla Nordin Fredrikson Prof. (also part time LU) Per Sthle von Schwerin Prof. (also part time LU) Ann Wennerberg Prof. Gunnel Svenster Prof. Ali Massih Prof. Julia Davies Prof. Per Jnsson Prof. Lennart Ljunggren Prof. Hkan Eriksson Prof. Vitaly Kocherbitov Assoc. Prof. Liselott Lindh Assoc. Prof. Anette Gjrloff-Wingren Assoc. Prof. Zoltan Blum Assoc. Prof. Liu-Ying Wei Assoc. Prof. Bertil Kinnby Assoc. Prof. Christina Bjerkn Assoc. Prof. Claes Wickstrm Assoc. Prof. Sergey Shleev Assoc. Prof. Alexei Iantchenko Assoc. Prof. Lars Ohlsson Dr. Tove Sandberg Dr. Maria Stollenwerk Dr.
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Anna Ketelsen Dr. Javier Sotres Dr.
Yuanji Cheng Dr. Jakob Blomqvist Dr.
JUNIOR RESEARCHERS AND POST-DOCS

Olga Santos, Dr. Laura Varas, Dr. Jessica Neilands, Dr. Luis Chavez de Paz, Dr. Ida Svendsen, Dr. Peter Falkman, Dr. Alejandro Barrantes, Dr. Jovice Bon Singh Ng, Dr. Ryo Jimbo, Dr. Viktor Andoralov, Dr. Pr Olsson, Dr. Peter Billsten, Dr. Justas Barauskas, Dr.
2.
PHD STUDENTS
Jildiz Hamit Eminovski Ulf Hejman Alma Masic Maria Pihl Sebastian Bjrklund (employed at LU) Anton Fagerstrm Yana Znamenskaya Adnan Safdar Christian Kindblom Marjan Dorkhan Kostas Bougas Magnus Falk Peter Lamberg Cathrine Albr Mariko Hayashi Lory Melin Svanborg Tuerdi Maimaitiyili Zahra El-Schich
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4. TECHNICAL AND ADMINISTRATIVE STAFF

Eva Nilsson Administrative Co-ordinator Ulrika Troedsson Technician Agnethe Henriksson Technician Madeleine Blomqvist Technician
5. MSc STUDENTS
BMMT Masters at HS 2011-2012
Gillian Xi Min Dao Linda Tran Jenifer Vallejo
Kouadio Christian Kouassi Amanda Skurzynska
Joynul Abedin Ameena Daftani Endale Asmare Hailu Rakibul Islam Lutfor Islam Abu Sayeed Khan Jabed Khandaker Shadi Movahed Bashiri
Shifa Saleem Selva Kumar Subramanian Mohammed B Sunmonu Inger Anne Tveit Surendra Vutti Shaheen Mohammad Syful Islam
Haddel Ali Shoker Joy Chia Ihab Dahi Payam Delfani
Susanna Tarasco Mohammad Zahir Uddin Petra Wicktor
In addition to these two-year Masters programmes, the Faculty of Odontology offers the Dentistry programme, which is a continuous, five-year programme to Masters level.
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MANAGEMENT AND BOARDS

Biofilms Research Center for Biointerfaces is managed on a daily basis by the Center Director and a Senior Researchers Forum, made up of the heads of the individual research groups and senior researchers from the Center. The Steering Group acts as a link to central Malm University and the community. The Steering Group comprises the Deputy Vice-Chancellor, the deans of the three faculties/schools involved in the research activities at the Center, representatives from Malm Universitys two research profiles Biointerfaces and Oral Health, and representatives from industry, Medicon Valley Alliance and other research institutions.
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Anna Holmberg: Dr., Director and Chairman, 2011Thomas Arnebrant: Prof. Gunnel Svenster: Prof. Ann Wennerberg: Prof. Tautgirdas Ruzgas: Prof. Julia Davies: Prof. Per Jnsson: Prof.
Liselott Lindh: Assoc, Prof. Bertil Kinnby: Assoc, Prof. Christina Bjerkn: Assoc, Prof. Anna Ketelsen: Dr. Johan Engblom: Assoc, Prof. (substitute) Claes Wickstrm: Assoc, Prof. (substitute)
SENIOR RESEARCHERS FORUM
Eva Engquist: Deputy Vice-Chancellor, Malm University, Chair Tapio Salonen: Dean, Faculty of Health and Society Naser Eftekharian: Head School of Technology, Malm University
Lars Bondemark: Prof., Dean, Faculty of Odontology Thomas Arnebrant: Prof., Head of Research Profile, Biointerfaces Gunnel Svenster: Prof., Representative Research Profile, Oral Health
STEERING GROUP
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Peter Nordstrm: Senior Project Manager, Medicon Valley Alliance Markus Johnsson: Dr., Senior Director, Pharmaceutical Development, Camurus AB
Ulf Brogren: CEO, Promimic AB Karl Obrant: Head of Research, Skne University Hospital
LIST OF RESEARCH ACTIVITIES

The research activities of the Center during 2011 can be presented under the following headlines. Projects are listed with partners and funding in brackets. In addition, we are also involved in three EU Interreg programmes; Medicon FISC, Valorisation of knowledge intensive ideas in the South Baltic area (SB-VALOR), and resund materials innovation community (-MIC). Center members are partners in the collaborative EU FP7-funded project Three-dimensional nanobiostructure-based self-contained devices for biomedical application (with Dr Sergey Shleev as co-ordinator) and in the EU FP7 Marie Curie Initial Training Network MagnIM.
1. EUCARYOTIC CELL-SURFACE INTERACTIONS

M icroscopy and digital holography for real-time applications and imaging: studying the cellular and molecular mechanisms of cancer cells (funded by MAS Cancer foundation) Biological responses to photoreactive hydrophilic nano-size structures (funded by VR)
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Hydrophilic and hydrophobic implant surfaces (funded by implant industry) H istological and 3-dimensional analysis of laminin coated polished ceramic implants (funded by Hjalmar Svensson Research Foundation)
The use of CaO as luting material and bone substitute (funded by VR) Facilitation of soft tissue healing upon implant treatment in patients with supressed healing ability (funded by Mah) Advanced surface characterization of new functional biomaterials (Ospol, funded by KKs)
T he effect of cholesterol-lowering drugs, statins, on cancer cells (funded by Mah) I nflammatory mechanism of Al-adjuvants (funded by Mah)
MOLECULAR TRANSPORT PHENOMENA

Adjuvants for products used in agriculture (AkzoNobel Surface Chemistry AB, funded by KKs (until Feb 2011) and Mah) Water a crucial factor in regulating biomembrane permeability (Physical Chemistry 1 (LU), funded by FLK, LU) Humectants and their mechanisms in skin (YKI AB, ACO Hud Nordic AB, Eviderm Institute AB, Q-Med AB, funded by KKs) M iniature biofuel cells for selfcontained bio-devices: electron transfer in three-dimensional nanobiostructures (funded by VR and EU) Grinfeld surface instabilities (funded by VR) Effect of a gradient in water chemical potential on buccal drug delivery (Food technology, LU, funded by Mah) Mucoadhesion A prerequisite or a constraint in nasal drug delivery (Nares AB, Bioglan AB, LTH, funded by KKs) Development of nanoparticle based enzymatic biofuel cells (Funded by SI) Medicon FISC (Foresight Institute + Support Centre), (funded by EU Interreg and Mah).
2.
MOLECULAR INTERACTIONS AT BIOINTERFACES

Symptomatic vs. asymptomatic atherosclerotic plaques (CRC, LU, funded in part by Mah) Biocompatibility of metals (Anordica AB & Arcam AB, funded by KKs)
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Hydration of mucous gel (funded by Mah) Topical hydrogen peroxide in wound healing (Bioglan AB, pilot project, funded in part by Mah) Membrane impedance spectroscopy as a tool to study skin barrier function and variability (Physical Chemistry 1 (LU), funded by FLK (LU) and KKs)
Development of novel multi-functional salivary substitutes for dry mouth syndrome patients (funded by the Swedish Laryng Foundation) Screening of phase behavior in the DOPS/DOPE/water system and effects on lipid morphology from a decrease in water chemical potential (funded by CLRF) Screening of different surface coatings including different API for enhanced wound healing (NIOM, Oslo, funded in part by Mah)
4. MICROBIAL BIOFILMS
Biologically induced stress corrosion crack growth (Arcam AB, funded by KKs (until Feb 2011) and Mah) Biofilm activity as a marker to identify patients at risk of caries (National Graduate Research School in Odontology, funded by VR and Mah) Mucins and microbial biofilms a symbiotic relationship for health (funded by Magnus Bergvalss Siftelse and Mah) Activities of microbial biofilms on bioactive implant surfaces (National Graduate Research School in Odontology, funded by VR and Mah) T he plasminogen activating system interaction with microorganisms (Funded by Mah)
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Study of biomarkers and microbial activity at dental implant sites exhibiting healthy bone and severe bone loss (Nobel Biocare, Funded by Mah) Biofilms on oral mucosal surfaces (Swedish Dental Association, Swedish Patent Revenue Fund for Research in Preventative Odontology) M ilk derived products a new strategy in oral biofilm control (ArlaFoods, funded by KKs) T he effect of probiotic lactobacilli on development of acid adaptation in dental biofilms (BioGaia, funded by KKs)
RESEARCH HIGHLIGHTS
GENETIC RESPONSES TO NANOSTRUCTURED CAP IMPLANTS
Although the application of nanostructures has been recognised as a possible enhancing factor for osseointegration, the detailed mechanisms have not been clarified to prove its initiative role in the osteogenesis cascade. In addition, the in vivo results presented in scientific publications are at times inconsistent, presenting the effect of nanostructures as significant, tendency, or insignificant to osseointegration. One possible explanation for this inconsistency could be that the mode of analysis, which was originally designed to investigate the effect of modification on the micro level, may be too rough for such coarse modification on the nano level. Furthermore, these conventional biomechanical and histological techniques may be technically sensitive and the results may vary depending on the operator. Hence, a coarse evaluation that can present absolute values may be necessary to further clarify the effect of nanostructures. In our study (Jimbo et al. 2011 J Dent Res), we evaluated the genetic responses to nanostructured implant surfaces. In brief, nanostructured calcium phosphate implants were placed in the rabbit tibia. After two and four weeks of insertion, the implants were removal torqued and the implant and the surrounding bone were extracted for gene expression analysis using the real time RT-PCR. The biomechanical results showed that at two weeks the removal torque values were significantly higher for the test (nano) compared to the control (turned surface without nanostructures), whereas at four weeks, there were no differences between the two groups. The results were in accordance with certain previous studies, where the effect of the nanostructure seemed to diminish at longer time points. However, the investigation using the gene expression approach revealed that even at four weeks, the genetic responses differed
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Figure1: Gene expression observed by the real-time PCR technique. It is evident that the nanostructured implants enhanced osteogenic marker expression.
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Project Partner: Promimic AB Genetic responses to nanostructured calcium-phosphate-coated implants. Jimbo R, Xue Y, Hayashi M, Schwartz-Filho HO, Andersson M, Mustafa K, Wennerberg A. J Dent Res 2011;90:1422-1427
significantly between test and control, and also revealed significantly higher expression of bone-related markers for the test group. This study showed that the effect of calcium phosphate nanostructures is
significant to osseointegration and that the nanostructures influence the gene expression levels of osteogenic markers. Based on this model, it may be possible to optimise implant nanostructures for further improvement in this field.
2. A WATER GRADIENT CAN BE USED TO REGULATE BIOPHYSICAL PROPERTIES OF SKIN: RELATIONSHIP BETWEEN IMPEDANCE AND DRUG PERMEABILITY
We aim to answer the question How do changes in the water gradient across skin affect the electrical impedance properties of the skin barrier? We make use of a novel experimental approach, which permits strict control of the boundary conditions that determine the water gradient. A further aim of the study is to exploit measurements using electrical impedance spectroscopy as a means of explaining diffusion properties of model drugs with different lipophilicity through skin. In conclusion, the skin membrane resistance, Rmem, and effective capacitance, Ceff, are affected by varying water gradient and to some extent the effect is reversible. The change in Rmem indicates that the ions inside SC become much more mobile upon hydration, resulting in a distinct decrease in Rmem. The increase in Ceff upon hydration indicates that the dielectric properties of the stratum corneum changes. The effect of water gradient on Rmem and Ceff is related to the flux behaviour of small molecular compounds through skin. When the skin membrane is more hydrated, it has a lower Rmem, a higher Ceff, and higher drug permeability, while the opposite is true when the membrane is less hydrated.
A.
B.
Figure1: (A) Schematic representation of the Franz cell equipped with four electrodes for electrical impedance measurements. (B) Equivalent electrical circuit used to fit the experi mental impedance data. Rsol is the resistance of the donor and receptor solution, Rmem is the membrane resistance, and CPE is a constant-phase element.
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The technique described above involving simultaneous assay of drug penetration and monitoring of impedance characteristics of skin was also used to assess permeability properties of biologically active compounds through
leaves and apple cuticle. Hopefully, measurement of impedance characteristics of biological membranes will help to account for the variability in penetration assays.
Project Partners: Galenica AB, Physical Chemistry 1, LU, Dermatology, LU, Innovator Skne AB, Akzo Nobel Surface Chemistry AB, Bayer Crop science A/G A water gradient can be used to regulate biophysical properties of skin: relationship between impedance and drug permeability; Bjrklund S, Engblom J, Dahi I, Ruzgas T; manuscript to be submitted in 2012
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3. FRICTION FORCE SPECTROSCOPY AS A TOOL TO STUDY THE STRENGTH AND STRUCTURE OF SALIVARY FILMS
The mouth is an entry point for a huge variety of substances into the human body. As a result, oral surfaces are constantly confronted with different chemical and mechanical challenges due to extrinsic factors that could lead, for example, to dental erosion. Salivary protein films are a major issue in the study of dental erosion as they cover all surfaces within the oral cavity and thus act as a protective barrier. We have used atomic force, microscopybased friction force spectroscopy (FFS) to study the strength of salivary films. Results revealed that salivary films have strengths in the order of hundreds of MPa. Moreover, our results show that the strength of the adsorbed salivary films increases dramatically with the water wettability of the underlying substrates, suggesting that hydrophobic materials in the oral cavity might be cleaned more easily of adsorbed salivary films. However, FFS also provides information on the structure of these films. From our experiments, it is inferred that on both hydrophobic and hydrophilic substrata these films can be described in terms of two different fractions, with only one of them being able to diffuse along the underlying substrata. FFS also sheds light on the mechanisms that lead to the loss of the protective function of salivary films when the pH of the surrounding medium is lowered a situation that mimics the intake of soft drinks. Specifically, lowering of pH causes desorption of some of the components of the films formed on hydrophobic surfaces, leading to weaker layers. In contrast, at a low pH, saliva no longer forms a layer on hydrophilic silica surfaces. Instead, an inhomogeneous distribution of amorphous aggregates is observed. Finally, reproducible differences are observed in results from experiments on films from different individuals, validating the technique as a tool for clinical diagnosis of the resistance to erosion of salivary films.
Friction Force Spectroscopy as a Tool to Study the Strength and Structure of Salivary Films. Sotres J., Lindh L., Arnebrant T. 2011 Langmuir. 27: 13692-13700
Figure1: Schematic drawing of an AFM tip scratching a salivary film. The sample corresponds to a real scratched salivary film imaged after FFS measurement.
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THE EFFECT OF LACTOBACILLUS STRAINS ON STREPTOCOCCUS MUTANS ACID ADAPTATION

Some bacteria are able to initiate an acid tolerance response (ATR) on exposure to sub-lethal pH levels which allows them to survive and continue to produce acids at low pH. Recently, there has been a great deal of interest in whether probiotic bacteria can be used to promote oral health and the aim of this study was investigate the effect of lactobacilli on acid adaptation in the model species, Streptococcus mutans.
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Figure1: a) LIVE/DEAD BacLight staining of S. mutans and Lactobacillus reuteri, b) phase contrast image showing the differences in morphology used to distinguish between the two species.
Streptococcus mutans UA159 and lactobacilli were allowed to adhere to the surface of a mini-flow cell for two hours (Figure 1) and an ATR was stimulated by exposure to pH 5.5 for two hours. ATR induction was tested by exposing the cells to pH 3.5 for 30 minutes and testing the viability using LIVE/DEAD BacLightTM staining and CSLM.
Jessica Neilands in collaboration with Biogaia AB.
Figure2: Graph showing how growth with different Lactobacillus species affects acid tolerance of S. mutans cells.
Our results showed that Lactobacillus casei UB17 and Lactobacillus reuteri PTA5289, but not Lactobacillus plantarum UA17, were capable of reducing acid adaptation in S. mutans (Figure 2).
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5. COMPOSITION AND PROTEASE ACTIVITY IN MICROBIAL BIOFILMS

Biofilm communities are highly organised structures composed of multiple bacterial species that interact with each other in order to survive. Synergistic coexistence of bacteria in multispecies biofilms has been proposed to play a crucial role in the development of chronic infections as many species become highly resistant to antimicrobial therapy and host defences. We have developed methodology to study the composition of multi-species communities using 16S rRNA FISH and confocal scanning laser microscopy (Figure 1a). This technology can be combined with fluorescent probes to investigate the physiological status, for instance activity of protease enzymes, of different cells within a biofilm (Figure 1b).
Figure1(a): Composition of a multi-species biofilm using four different 16S rRNA probes for Streptococcus gordonii (green), Lactobacillus salivarius (red), Enterococcus faecalis (pink) and Actinomyces naeslundii (blue). Figure1(b): Species-specific 16S r-RNA probes in combination with a fluorescent protease substrate (FITCcasein) showing the physiological status of individual cells of each bacterial species within a 4-species biofilm.
Images: Luis Chvez de Paz
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Fluorescent substrates can also be used on multi-species biofilms developed in vivo (dental plaque) to investigate the physiological activity of the cells (Figure 2a & b).
Image: Claes Wickstrm, Bertil Kinnby, Julia Davies, Gunnel Svenster, Wickstrm C, Herzberg MC, Beighton D, Svenster G. (2009) Microbiology 155:2866-72
Image: Bertil Kinnby Figure2: Plaque biofilms showing proteolytically active (green) and non-proteolytically active cells.
These results show that this is a powerful technique for investigation of the metabolic and physiological properties of multi-species biofilms.
Method development: Claes Wickstrm, Bertil Kinnby, Julia Davies and Gunnel Svenster in collaboration with Nobel Biocare AB.
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6. SURFACE-ASSOCIATED PLASMINOGEN AFFECTS THE POPULATION DIVERSITY IN MIXED-SPECIES BIOFILMS

Infected root canals contain necrotic tissue as well as tissue fluids derived from inflammatory reaction around the root apex. Under such circumstances, plasma components, such as plasminogen, may coat the root canal walls and act as a conditioning film to which bacteria can adhere and grow. We have studied the effect of surface-associated plasminogen on the structure and species distribution of a four-species community recovered from infected root canals (Lactobacillus salivarius, Streptococcus. gordonii, Enterococcus faecalis and Actinomyces naeslundii). Bacteria were grown on plasminogenor albumin-coated flow chambers and monitored for 96 h with confocal laser scanning microscopy. BacLight LIVE/ DEAD staining was used to assess cell viability and 16S rRNA fluorescence in situ hybridisation (FISH) used to study the distribution of the species. After 24 h, no significant difference was seen in the relative amounts of the four species on the two different coatings (Figure 1). Adherence of S. gordonii to the plasminogen-coated surfaces was inhibited by epsilon amino-caprioic acid, indicating that binding is mediated via lysine residues in the molecule.
Figure1: 16S rRNA-FISH staining showing the distribution of S. gordonii (green), L. salivarius (red), A. naeslundii (blue) and E. faecalis (pink) on plasminogencoated surfaces (upper panel) or albumin-coated surfaces (lower panel) after 24 h and 96 h. Kinnby B, Chvez de Paz LE, DaviesJR, Svenster G. Protein coating influences biofilm formation by a root canal consortium. To be submitted to Microbiology.
After 96 h, the dominant species on the plasminogen-coated surface was S gordonii, whereas on the albumin-coated surface, no single species dominated. Thus, surface-associated plasminogen appears to affect the special distribution of root canal bacteria in mature biofilms.
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HUMAN SALIVARY MUCIN MUC5B-COATED SURFACES ENHANCE PROTEASE ACTIVITY

A major function of the salivary pellicle on oral surfaces is to promote colonisation of the commensal microbiota by providing binding sites for adherence. Streptocoocus mitis is an early coloniser of the oral cavity while Streptococcus mutans represents a later coloniser. As biofilms develop, bacteria undergo adaption to survive in their habitat. One example of this is the expression of enzymes to allow exploitation of salivary proteins and glycoproteins as a nutrient source. In this study, adherence and protease activity of S. mitis biovar 2 and S. mutans were investigated in a flow cell model in the presence of different populations of surfaceassociated salivary proteins (Figure 1).
7.
Figure1: Ibidi flow cell system with a continuous flow of nutrients allows biofilm formation. Confocal microscopy is used to visualise adherence and protease activity.
Streptococcus mitis biovar 2 adhered well to surfaces coated with both an MUC5Benriched fraction or a pool containing MUC7, gp340, amylase, lactoferrin, lysozyme, cystatin, statherin and IgA, whereas adherence of S. mutans was poor. These differences in adhesion may explain in part why S. mitis is an early coloniser of oral surfaces while S. mutans colonises later.
Figure2: Protease activity in S. mitis biovar 2 is upregulated following contact with a surface conditioned with human salivary MUC5B mucin. Cells were visualised in a CLSM after staining with FITC Casein and SYTO 62. All cells are stained red and the green halo indicates protease active cells.
In conjunction with adherence to salivary proteins, we demonstrated a rapid up-regulation of proteolytic activity by both streptococcal species. This suggests that both species can sense surface-associated salivary proteins and respond by up-regulating proteases and/or proteolytic activity. This may represent a mechanism for the generation of nutrients through the degradation of salivary proteins.
Kindblom C, Davies JR, Herzberg MC, Svenster G and Wickstrm C. Surface-associated salivary proteins promote proteolytic activity in Streptococcus mitis biovar 2 and Streptococcus mutans. Submitted to Molecular Oral Microbiology December 2011.
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8. CAN BACTERIA SENSE AND RESPOND TO MUCINS ON SURFACES?

Mucins and commensal microorganisms have a strong tendency to associate with surfaces. The formation of adherent microbial communities prevents colonisation by pathogenic bacteria. This study is based on the hypothesis that early colonisers, such as Streptococcus gordonii, interact with mucins, resulting in sequential expression of cell wall and other surface proteins that are fundamental to adherence and survival of bacteria in the mouth. The primary aim of this study was to establish the cell surface proteome of S. gordonii, including cell wall proteins (Figure 1). Protein extraction procedures were developed for cell surface and cell wall proteins and two-dimensional electrophoresis was optimised to allow separation of the cell wall proteins. Proteins were identified using mass spectrometry.
Figure1: Cell wall proteins of Streptococcus gordonii.
Changes in cell surface protein expression during interaction with mucin-coated surfaces were then investigated (Figure 2).
2DE: Agnethe Henriksson Figure2: Surface proteins of S. gordonii in the bulk phase (left) and after adherence to a mucin-coated flow cell. Mucins were prepared from saliva and used to coat flow cell surfaces to which bacteria were allowed to adhere for two hours.
Method development: Claes Wickstrm, Julia Davies, Mark Herzberg, Agnethe Henriksson, Gunnel Svenster.
Four LPXTG-linked proteins (recognised as putative adhesins), glyceraldehyde3P dehydrogenase (gapdh) and phosphopyruvate hydratase (pph) dominated the cell wall proteome. Proteomic analysis of surface proteins revealed that most were down-regulated, except for superoxide dismutase, which showed significant up-regulation on contact with mucin. These data suggest a role for superoxide dismutase for bacterial survival in initial biofilms.
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A NOVEL SALIVA-BINDING ADHESIN FROM STREPTOCOCCUS ORALIS

The use of titanium dental implants to treat tooth loss has increased rapidly in recent years. If the implant becomes exposed to the oral cavity, bacteria will adhere to the surface, leading to the formation of microbial biofilms, which can induce peri-implant inflammation. Streptococcus oralis is an early coloniser of oral surfaces and has been recovered from titanium surfaces in vivo. The purpose of this study was to examine the effect of a saliva- or serum-derived coating on adherence of clinical strains of S. oralis to titanium. Three different strains of S. oralis were tested and adherence was studied using a flow cell system and confocal laser scanning microscopy. The results indicated that while serum did not promote binding of any studied S. oralis strains, a saliva coating increased adherence in two out of three strains tested.
9.
Figure1: 2DE of cell wall proteins from three strains of S. oralis. Arrows indicating LPXTG-linked protein. Reference proteins are circled: 1, DnaK; 2, GroEL; 3, trigger factor.
2DE: Agnethe Henriksson
In order to investigate the mechanisms of binding to saliva, bacterial cell wall proteins were isolated and analysed using proteomics (Figure 1). This showed that S. oralis strains bound to saliva expressed an LPXTG-linked protein, which was not present in the
non-adherent strain. Consequently, strains of S. oralis differ in their capacity to bind to saliva-coated titanium and we propose that this is due to differential expression of a novel adhesin.
Dorkhan M., Chvez de Paz L. E., Skep M., Svenster G. and Davies J. R. (2012). Effects of saliva or serum coating on adherence of Streptococcus oralis strains to titanium. Microbiol. 159:390-397.
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SCIENTIFIC INSTRUMENTATION AND EQUIPMENT

1. OPTICAL MICROSCOPY
(Contact person: Vitaly Kocherbitov) A Zeiss Axioplan Universal microscope equipped with differential interference contrast and a High Resolution Microscopy Camera AxioCam MRm Rev. 3 FireWire, Illuminator HBO 100 as well as a 100W mercury short-arc lamp and a system of filters to allow fluorescence microscopy observations. The microscope is also equipped with a high-sensitivity SIT video camera and an image processor, AxioVision 4 and Macintosh-based image analysis software.
2. IN VITRO DIFFUSION EQUIPMENT

(Contact person: Johan Engblom) Three alternative items of thermostated in vitro diffusion equipment are available in house, two set-ups with flow-through cells (15 + 7 cells) [R.L. Bronaugh & R.F. Stewart, J. Pharm. Sci. 74 (1985) 6467] and 10 static Franz cells [Franz T, J. Invest. Dermatol. 64 (1975) 190-195]. Solute diffusion over skin, oral mucosa, nasal mucosa, nails, plant cuticle and synthetic membranes has been studied and assayed spectrophotometrically (online) or using HPLC-UV. Electrical impedance spectroscopy has been appended to the Franz cells as a tool to explain diffusion properties of solutes through these membranes.
3. SWAXD
(Contact persons: Justas Barauskas, Johan Engblom) Small and Wide Angle X-Ray Scattering. Kratky compact camera with line collimation (slit focus), equipped with two linear MBraun detectors (small and wide angle). Temperaturecontrolled sample stages (0-70C or 25-300C). Powder (semi-solid/solid) or capillary (liquid) sample holders, as well as a flow-through capillary sample holder.
4. DSC
(Contact person: Vitaly Kocherbitov) Differential scanning calorimeter DSC1 from MettlerToledo, equipped with an intracooler TC100 and HSS8 sensor. Temperature range: -90C to +550C. The calorimeter can be used
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to study solid, soft and liquid substances. Typical sample masses are 2-10 mg. The DSC can be run in a modulation regime to resolve reversing and nonreversing heat effects.
ELLIPSOMETER
(Contact person: Alejandro Barrantes) An automated Rudolph thin film ellipsometer (type 43603-200E, Rudolph Research, USA) equipped with a xenon arc lamp as a light source; an interference filter with UV and infrared blocking (Melles Griot, The Netherlands) for working at a wavelength of 4429 . The experimental set-up is based on null ellipsometry in the PCSA arrangement. The components of the ellipsometer are controlled by means of the Ellipso software, which automatically measures the ellipsometric angles Psi and Delta, allowing the calculation of the thickness, refractive index and adsorbed mass of the growing films. To perform experiments in liquid media, a peristaltic pump (Ole Dich Instruments) is used. The temperature is controlled by means of a Julabo 5B water bath. Data analysis is performed using the Ellipsometry software by Plamen Petrov.
5.
e-SEM
(Contact persons: Zoltan Blum, Justas Barauskas) Scanning Electron Microscope, SEM, Zeiss EVO LS10. Apart from standard modern SEM features, i.e. 5 nm pointto-point resolution at high vacuum and fully conducting samples, the EVO LS10 also offers environmental control (hence EVO). By controlled water vapour introduction, with or without the added benefits of a Peltier Coolstage, fully hydrated samples can be imaged with accurate sample temperature control. Owing to the high output LaB6 filament and the beam sleeve technology, the loss in resolution is only tenfold. The instrument is equipped with the appropriate detectors as well as an INCA EDX microanalysis system.
6.
QCM-D
(Contact person: Alejandro Barrantes) The Q-Sense E4 quartz crystal microbalance is equipped with the E4 measuring chamber that allows up to four experiments in the four temperaturecontrolled cells to be run simultaneously. Changes in frequency and dissipation are registered by means of the E-series electronic unit, which is also responsible for temperature control. These changes are related to the adsorbed wet mass and the viscoelastic properties of the films adsorbed onto the sensor. The QCM-D is also equipped with an electrochemistry and an ellipsometry module, enabling the simultaneous measurement of the electrochemical and optical properties of the adsorbed films. There is a four-channel IPC peristaltic pump (Ismatec). Data analysis is performed using the Q-Tools software (Q-Sense).
7.
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8. DLS/ZETA SIZING SYSTEM

(Contact person: Peter Lamberg) A combined Electrophoretic Light Scattering/Dynamic Light Scattering Zeta potential/particle sizing system with a 5 MW 632.8 nm HeNe laser, two insertable electrodes for high or low voltage, covering possible electrical field strengths between 0.25-250 V/cm, and an adjustable temperature range of 4-100C. The instrument has also been fitted with a Thermaltake Bigwater 760is liquid cooling system, to prevent cuvette fogging at high temperatures.
9. GONIOMETER
(Contact person: Vitaly Kocherbitov) Kruss Goniometer DSA100 Drop Shape Analyser System for measurement of static and dynamic contact angles. The goniometer is equipped with a single dosing system DS3200/3201. Temperature is controlled in the range 5-90C using a TC30 measuring chamber. Humidity is controlled using a HC10 humidity chamber.
10. AFM
(Contact persons: Javier Sotres, Thomas Arnebrant) A Multimode SPM equipped with a Nanoscope VIII control unit (Bruker AXS). The SPM is also equipped with a PicoForce Unit, a Heater Unit, and the ScanAsyst and Peak Force Tapping software (Bruker AXS). In addition, a digital oscilloscope (Tektronix TDS 2022C) and a Nikon optical microscope are connected to the system.
11. IMPEDANCE MEASURING EQUIPMENT

(Contact person: Tautgirdas Ruzgas) Franz cells ( = 0.90 cm, V = 6 ml, PermeGear, Hellertown, PA, USA) equipped with four electrodes and connected to a potentiostat from Ivium Technologies (Eindhoven, The Netherlands) are used to study impedance characteristics of membranes, including skin, leafs, etc. Two platinum wires serve as working and counter electrodes and two Ag/AgCl/3M KCl electrodes from World Precision Instruments (Sarasota, FL, USA) are used as sensing and reference electrodes. The equipment allows impedance measurements at frequencies up to 6 MHz.
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DROP VOLUME APPARATUS

(Contact person: Tautgirdas Ruzgas) Drop Volume Tensiometer TVT 2 consists of a step motor-driven syringe, including a needle for droplet formation, set up for optical droplet falling registration, and software to operate hardware and calculate the surface tension of liquid. This apparatus is used to determine the surface tension of liquids.
12.
LANGMUIR SURFACE BALANCE

(Contact person: Johan Engblom) KSV 5000 is a programmable Langmuir and Langmuir-Blodgett instrument for automated Langmuir film experiments and for deposition of normal or alternating multilayers onto solid substrates. Surface pressure is measured using the Wilhelmy plate method.
13.
PARTICLE ELECTROPHORESIS
(Contact person: Tautgirdas Ruzgas) Particle micro-electrophoresis apparatus Mark II (Rank Brothers, Cambridge, UK) consists of a flat, electrophoretic cell equipped with two platinum electrodes and optical microscope. The apparatus can be used to determine particle electrophoretic mobility in solution. The mobility can be used to assess particle zeta potential and surface charge density. The apparatus allows measurements with particles larger than 0.3 m in diameter.
14.
RHEOMETER
(Contact persons: Vitaly Kocherbitov, Alejandro Barrantes) Rheometer Bohlin CVO 100 Digital equipped with Peltier Cylinder C25. The rheometer system is suitable for research, product development and quality control. The rheometer is equipped with 20 mm parallel plate, cone 4/40 and coaxial cylinder measuring systems.
15.
SORPTION CALORIMETER
(Contact person: Vitaly Kocherbitov) A double-twin sorption calorimeter for the study of hydration of powders and soft materials. The channel diameter is 28 mm. The calorimeter is equipped with two sorption cells, each consisting of a sorption chamber (on top) and a vaporisation chamber (bottom). The chambers are connected by tubes with varying diameters to control the vapour flow. The double twin calorimeter is inserted into an isothermal TAM 2277 calorimeter from Thermometric, equipped with a nanoamplifier.
16.
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17. INTERFEROMETER
(Contact persons: Ryo Jimbo, Ann Wennerberg) White light microscopy, horizontal resolution 0.3 m, vertical 0.1 nm, equipped with a CCD camera, operating also in phaseshift mode. The main application is topographical characterisation on the m and sub-m level.
18. FLUORCHEM E
(Contact person: Anette Gjrloff Wingren) High-performance Western blot and gel imaging. A self-contained digital darkroom, compact design with stateof-the-art CCD optical technology, an integrated computer and simplified touch screen control. The FluorChem E is built with imaging speeds and a dynamic range that surpass film for low femtogram detection of proteins and DNA in chemiluminescent, colorimetric and UV fluorescent gels and blots.
19. MICROBIAL DIAGNOSTICS; SPECIES AND STRAIN LEVELS

(Contact person: Gunnel Svenster) Autoclaves Anaerobic chamber Fluorostar Incubators Spectrophotometer PCR Centrifuges
20. BIOFILM FORMATION, INCLUDING ADHERENCE, DETACHMENT, POPULATION DIVERSITY AND PHENOTYPIC SWIFT
(Contact person: Gunnel Svenster) Epifluorescence microscope Confocal laser scanning microscope Flow cells with controlled continuous flow Cell cultures
21. PURIFICATION OF MUCINS AND PROTEINS

(Contact person: Gunnel Svenster) FPLC Preparative electrophores Ultracentrifuge
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PROTEOMICS, INCLUDING RADIOACTIVE LABELLING OF PROTEINS

(Contact person: Gunnel Svenster) Multiphor Two-dimensional electrophoresis Sonicator
22.
IDENTIFICATION OF PROTEINS
(Contact person: Gunnel Svenster) Immunohistochemistry Western blots ELISA
23.
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WORKSHOPS AND CONFERENCES ORGANISED BY THE CENTER

During 2011 the Center launched the 7th Annual Workshop. The focus this year was Non-Invasive Biomedical Monitoring and Devices and the event attracted 100 participants from different universities as well as industry. The workshop was preceded by a PhD student day with Professor Pentti Tengvall, Gothenburg University, and Professor Marie Wahlgren, Lund University, as mentors. The Center also launched an Inspiration Day, an interdisciplinary workshop for sharing research progress, at Malm University. The event attracted 50 participants from industry, both partners and Center members. Vitaly Kocherbitov, who is co-ordinating the project Hydration of nanostructured and biological materials, brought researchers together for a symposium at Malm University. Around 30 researchers from Malm University, Vilnius University, St. Petersburg State University and Lund University came together to exchange experience within the field of hydration research. Johan Engblom organised a partner meeting for the project Humectants and their mechanisms in skin at YKI AB. Johan Engblom also arranged a workshop with AkzoNobel and Bayer Crop Science at Malm University. Peter Falkman arranged a training session on Intellectual property rights at Malm University. Our internal seminars were organised by Tautgirdas Ruzgas, Anette Gjrloff Wingren, Julia Davies, and Ann Wennerberg. Gunilla Nordin Fredrikson was responsible for the seminars at the Clinical Research Center, Lund University.
SKIN, SALIVA AND TEARS

Each year, Biofilms - Research Center for Biointerfaces holds a workshop that addresses researchers, industry and other principal figures in the biomedical field. This year, the topic was Non-invasive Biomedical Monitoring and Devices. The annual Biofilms workshop this year attracted almost 100 guests from different universities and companies. During the two-day workshop, a wide range of interesting lectures were held on the topic of non-invasive biomedical monitoring and devices with a special focus on skin, saliva and tears. This is an area in which research is moving rapidly forward. We are very happy to present our research in the field and at the same time have the opportunity to listen to many interesting lecturers from industry, says Anna Holmberg, Director of Biofilms - Research Center for Biointerfaces.
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During the workshop, prominent researchers as well as representatives from industry talked about new methods for measuring, monitoring and making diagnoses from skin, saliva and tears. Erik Thomsen, researcher at DTU Nanotech, lectured on the development of an electronic patch that can measure heart beat rate, oxygen saturation, and blood pressure. John McCormac, Mini FAB, explained about a device that can be used to sample small volumes of tears and that can be used to make a diagnosis. From the Faculty of Health and Society, researcher Sergey Shleev talked about the development of an electrochemical bio-device that can be used to meas-
ure different substances in tears. Tautgirdas Ruzgas, also from the Faculty, lectured on how to study skin permeability using impedance spectroscopy. This is the third time I have participated and I find the workshop very interesting. The wide range of lectures provides a good insight into what is new, says Jan Alenfall, CEO of DemaGen. Anna Karin Morn, product developer at Galenica, is also very positive. The interdisciplinary arrangement and the wide range of applications that have been presented are very interesting, she says.
Malm University Newsletter, 2011-10-31
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PHD THESES SUPERVISED BY CENTER MEMBERS

THESES DEFENDED: ory Melin Svanborg L Mah (Supervisors Ann Wennerberg, Martin Andersson):
On the importance of nanometer structures for implant incorporation in bone tissue (Dissertation 2011-06-01)
Daniel Kolbus LU (Supervisor Gunilla Nordin Fredrikson):

Adaptive immune responses in atherosclerosis (Dissertation 2011-03-17)
Christel Larsson Mah (Supervisor Per Vult von Steyern, Ann Wennerberg):
Zirconium dioxide based dental restorations (Dissertation 2011-05-20)
Maria Wigren LU (Supervisor Gunilla Nordin Fredrikson):

Protective Immunity in Atherosclerosis (Dissertation 2011-04-15)
Jildiz Hamit Eminovski Mah (Supervisors Thomas Arnebrant and Krister Eskilsson (Kemira AB)):
Interactions of biopolymers and metal complexes at biological interfaces (Dissertation 2011-09-15)
Katarina Berg LU (Supervisor Gunilla Nordin Fredrikson):

Lyso-PC and macrophage interplay (Dissertation 2011-05-20)
Maria Pihl Mah (Supervisors Gunnel Svenster, Bertil Kinnby, Thomas Arnebrant):
Microbial Biofilms on Peritoneal Dialysis Catheters (Dissertation 2011-03-25)
THESES IN PROGRESS:
Kostas Bougas Mah (Supervisors Ann Wennerberg, Pentti Tengvall (GU), Victoria Franke Stenport (GU)):
Protein coat and bone incorporation (2009-)
Liselott Ellmarker-Lfquist Mah (supervisors: Liselott Lindh, Bjrn Sderfeldt):

A Clinical and subjective evaluation of single implant treatments. A retroscpective study. (2009-)
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Ulf Hejman Mah (Supervisors Per Sthle, Christina Bjerkn):

Biologically induced stress corrosion (2005-, Lic thesis defended 2010, doctoral thesis will be defended autumn 2012)
Yana Znamenskaya Mah (Supervisors Vitaly Kocherbitov and Johan Engblom):

Hydration of mucous gel (2009-)
Adnan Safdar LU (Supervisors Liu-Ying Wei, Per Sthle):

Biocompatibility of ion beam melted materials (2008-, Lic thesis defended Dec 2010, doctoral thesis will be defended spring 2012)
Christian Kindblom Mah (Supervisors Gunnel Svenster, Claes Wickstrm, Madeleine Rohlin):
Biofilm activity as a marker to identify patients at risk mechanisms underlying microbial stress tolerance (2009-)
Alma Masic Mah (Supervisors Per Sthle, Johan Helsing):

Mathematical modeling of biofilms (2007-, doctoral thesis will be defended spring 2012)
Marjan Dorkhan Mah (Supervisors Julia Davies, Gunnel Svenster, Ann Wennerberg):
Activities of microbial biofilms on bioactive implant surfaces (2009-)
Sebastian Bjrklund LU (Supervisors Emma Sparr (LU), Johan Engblom (Mah) and Krister Thuresson (Camurus AB)):
Water - a crucial factor in regulating biomembrane permeability (2008-)
Magnus Falk Mah (Supervisors Sergey Shleev, Tautgirdas Ruzgas,):

Three-dimensional nanobiostructure-based self-contained devices for biomedical application (2009-)
Anton Fagerstrm Mah (Supervisors Johan Engblom, Vitaly Kocherbitov and Karin Bergstrm (AkzoNobel)):
Bioavailability of active ingredients used in agriculture (2009-)
Jon Lind LTH (Supervisors Ali Massih, Christina Bjerkn):

Methods for evaluation of evaluation of the hydride embrittlement of Ni-based super alloys (2009-)
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THESES IN PROGRESS:
Tuerdi Maymaytilli LTH (Supervisors Christina Bjerkn, Per Sthle):

Influence of plastic deformation on the formation and growth of embritteling metal hydrides (2009-)
Caitriona Grnberg LU (Supervisor: Gunilla Nordin Fredrikson):

The role of IL-16 in atherosclerosis development (2010-)
Mariko Hayashi (Supervisors: Liselott Lindh, Ann Wennerberg, Ryo Jimbo):

Biological Responses to Amphiphilic Nano-size Structures (2010-)
Polyxeni Mantani LU (Supervisor: Gunilla Nordin Fredrikson):

Atheroprotective humoral and T cell immune responses against oxidized LDL (2010-)
Peter Lamberg Mah (Supervisors: Tautgirdas Ruzgas, Thomas Arnebrant):

Three-dimensional (3D) bioelectrochemical systems assembled from nanoparticles (2010-)
Sara Rattik LU (Supervisor: Gunilla Nordin Fredrikson):

The role of IL-22 in atherosclerosis development (2011-)
Cathrine Albr Mah (Supervisors: Johan Engblom, Vitaly Kocherbitov, Marie Lodn, Robert Corkery):
Humectants and their effects on skin barrier function a mechanistic approach (2010-)
Zahra El-Schich Mah (Supervisors: Anette Gjrloff Wingren, Anna Ketelsen, Gunilla Nordin Fredrikson):
Microscopy and digital holography for real-time applications and imaging: studying the cellular and molecular mechanisms of inflammatory cells and cancer cells (2011-)
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COLLABORATIVE PARTNERS OF THE CENTER

INDUSTRY PARTNERS
ACO Hud Nordic AB Akzo Nobel AB Anordica AB AnoxKaldnes AB Acram AB Arla Foods AB Biogaia AB Bioglan AB Bioinvent International AB Camurus AB Euro-Diagnostica AB Eka Chemicals AB Eviderm Institute AB Galenica AB Gambro Lundia AB InnoScandinavia AB Innovator Skne AB Kemira AB Layer Lab AB Magle AB Medeon AB Medicon Valley Alliance MiniFAB, Australia Nares AB NIL Technology A/S, Denmark Nobel Biocare AB Novosense AB Novozymes A/S, Denmark Obducat Technologies AB Phase Holographic Imaging AB Promimic AB PVA-MV AG, Germany Q-Med AB QuNano AB Quantumwise A/S, Denmark Snemca Moteur, France Stora Enso AB Studsvik Nuclear AB TetraPak AB Volvo Aero AB YKI AB Ospol AB
1.
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2. ACADEMIC COLLABORATORS
COLLABORATIONS WITH OTHER UNIVERSITIES AND RESEARCH INSTITUTIONS IN SWEDEN Prof. em. Kre Larsson, Camurus Lipid Research FoundationLund University Prof. Gunnar Bergenholtz, Dept. of Endodontics University of Gothenburg Prof. Gunnar Dahln, Dept of Oral Microbiology, University of Gothenburg Prof. Ingegerd Johansson, Dept of Cariology, Ume University Prof. Artur Schmidtchen, Department of Clinical Sciences, Lund University Prof. Swante Twetman, Specialist Clinic for Oral Health Care, Hallands Lns Landsting, Halmstad Prof. Kristian Reisbeck, Dept of Medical Microbiology, Lund University Prof. Per Claesson, The Royal Institute of Technology and Surface Chemistry Institute, Stockholm Prof. Lo Gorton, Biochemistry, Lund University Prof. Christer Hansson, Dermatology, Lund University Prof. Martin Malmsten, Pharmacy, Uppsala University Prof. Jan Nilsson, CRC UMAS, Lund University Prof. Tommy Nylander, Physical Chemistry 1, Lund University Prof. Per Uvdal, MAX lab, Lund University Prof. Martin Andersson, Dept Applied Chemistry, Chalmers University of Technology Prof. Pentti Tengvall, Dept Biomaterials, Sahlgrenska Academy, Gothenburg University Prof. Tomas Albrektsson, Dept Biomaterials, Sahlgrenska Academy, Gothenburg University Prof. Marie Wahlgren, Food Technology, LTH, Lund University Prof. Lars Montelius, Solid State Physics, Lund University Prof. Ulf Ryde, Department of Theoretical Chemistry, Lund University Prof. Olle Melander, CRC, Lund University Assoc. Prof. Lars Norln, Cell- and Molecular biology, Karolinska Institute Assoc. Prof. Ola Bergendorff, Dermatology, Lund University
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Assoc. Prof. Emma Sparr, Physical Chemistry 1, Lund University Assoc. Prof. Peter Siesj, Department of Clinical Sciences, BMC, Lund University Dr. Karina Persson, Inst. of Odontology, Ume University Dr. Christer Wingren, LTH, Lund University Dr. Lars G Petersson, Specialist Clinic for Oral Health Care, Hallands Lns Landsting, Halmstad Dr. Yngve Cerenius, MAX lab, Lund University Dr. Adam Feiler, The Royal Institute of Technology and Surface Chemistry Institute, Stockholm Dr. Isabel Goncalves, Ola Bergendorff, CRC UMAS, Lund University Dr. Robert Corkery, The Royal Institute of Technology and Surface Chemistry Institute, Stockholm Dr. Anna Westerlund, Odontology, Gothenburg University Dr. Ivan Maximov, Solid State Physics, Lund University Dr. Jenny Liao Persson, Center for Molecular Pathology, Lund University Dr. Birgit Brandner, Surface Chemistry Institute, Stockholm Dr. Toms Plivelic, MAX lab, Lund University Bjrn Svensson, Specialist docent, Clinic for Oral Health Care, rebro Lns Landsting, rebro Per Rabe, Specialist Clinic for Oral Health Care, Hallands Lns Landsting, Halmstad Brnemark Clinic, Gothenburg
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INTERNATIONAL COLLABORATIONS
Prof. Arunas Ramanavicius, Center of Nanotechnology and Material Science, Faculty of Chemistry, Vilnius University, Vilnius, Lithuania Prof. Juozas Kulys, Department of Enzyme Chemistry, Institute of Biochemistry, Vilnius, Lithuania Prof. Renata Bilewicz, Faculty of Chemistry, University of Warsaw, Warsaw, Poland Prof. em. Ian Hamilton, University of Manitoba, Dept of Oral Biology, Winnipeg, Canada Prof. David Beighton, Guys Kings and St Thomas Dental Institute, JointMicrobiology Research Unit, London, UK Prof. Mark Herzberg, Department of Microbiology, University of Minnesota, Minneapolis, USA Prof. Jukka Meurman, Dept of Oral and Maxillofacial Diseases, Helsinki University Central Hospital, Helsinki, Finland Prof. Dennis Cvitkovitch, Dept of Microbiology, University of Toronto, Canada Prof. Jeannine Brady, Dept of Oral Biology, University of Florida, Gainesville, USA Prof. Phil Marsh, Health Protection Agency, Porton Down, UK Prof. Deirdre Devine, Leeds Dental Institute, UK Prof. Leslie Banks-Sills, Cornell University, Ithaca, NY, USA and University of TelAviv, Israel Prof. Jon E Dahl, NIOM, Oslo, Norway Prof. Iwona Beech, University of Portsmouth, UK Prof. Regine Willumeit, GKSS Research Centre, Geesthacht, Germany Prof. Christopher Exley, Keele University, UK Prof. Takashe Sawase, Dept Prosthodontics, University of Nagasaki, Japan Prof. Wolfgang Schuhmann, Ruhr-Universitt Bochum, Germany Prof. Edmond Magner, University of Limerick, Ireland Prof. Dietmar Haltrich, Universitt fr Bodenkultur Wien, Austria Prof. Phil Bartlett, The University of Southampton, UK Prof. Kamal Mustafa, University of Bergen, Norway Prof. Alexander Yaropolov, Inst. of Biochemistry, Moscow, Russia
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Assoc. Prof. Marie Ranson, School of Biological Sciences, Scientific Director Cancer, Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, Australia Assoc. Prof. Duncan Sutherland, iNano Centre, Aarhus University, Denmark Assoc. Prof. Tim Tolker-Nielsen, University of Copenhagen, Denmark Assoc. Prof. David Thornton, University of Manchester, UK Assoc. Prof. Pablo Galindo, Department of Oral surgery and Implant Dentistry, Granada University, Spain Dr. Vladimir A. Serezhenkov, N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia Dr. Xiaoju Wang, Laboratory of Inorganic Chemistry, Process Chemistry Centre, bo Akademi University, Turku, Finland Dr. Hirotoshi Matsumurad, Department of Biomaterials Sciences, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan Dr. Roland Ludwig, Research Centre Applied Biocatalysis, Graz, Austria Dr. Lubomr Rulek, Institute of Organic Chemistry and Biochemistry, Gilead Sciences Research Center at IOCB, Academy of Sciences of the Czech Republic, Praha, Czech Republic Dr. Peter Wejse, ArlaFoods, Aarhus, Denmark Dr. Sergei Lobov, Browitt Nanoparticle Lab, Dept of Applied Mathematics, Australian National University, Canberra, Australia Dr. Miguel Alcalde, Consejo Superior de Investigaciones Cientficas, Applied Biocatalysis group, Spain Dr. Antonio L. De Lacey, Consejo Superior de Investigaciones Cientficas, Bioelectrocatalysis group, Spain Dr. Donald Leech, National University of Ireland, Galway, Ireland Dr. Paulo G. Coelho, Department of Biomimetics, New York University, USA Dr. Yuusuke Kozai, Department of Radiology, Kanagawa Dental University, Japan Dr. Humberto Schwartz, Sao Paolo, Brasilien Dr. Jan-Tore Samuelsen, NIOM, Oslo, Norway
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LIST OF PUBLICATIONS AT CENTER FROM 2011 AND ONWARDS

All titles listed under journal articles, review papers, and book chapters have been or are currently subject to peer review. Center publications for 2010 can be found at http://www.mah.se/biofilms
1. JOURNAL ARTICLES AND INVITED REVIEW PAPERS IN JOURNALS

Bergenzaun, L., Gudmundsson, P., hlin, H., Dring, J., Ersson, A., Ihrman, L., et al. (2011).
Assessing left ventricular systolic function in shock: Evaluation of echocardiographic parameters in intensive care. [elektronisk resurs]. Critical care;4 Biomed Centralw Ltd.
Beyl Y, Guschin D A, Shleev S, Schuhmann W, A chloride resistant high potential oxygen

reducing biocathode based on a fungal laccase incorporated into an optimized Os-complex modified redox hydrogel. Electrochemistry Communications, 2011, 13(5), 474-476.
Bjrkbacka H, Fredrikson GN and Nilsson J. Beyond CRP - looking for biomarkers for
immune modulators of inflammation, J Am Coll Cardiol, pending revision.
Bjrklund, Sebastian and Wads, Lars A calorimetric method to determine water activity,
REVIEW OF SCIENTIFIC INSTRUMENTS 82, 114903 (2011)
Bonfante EA, Granato, R, Marin C, Jimbo R, Giro G, Suzuki M, Coelho, PG. Biomechanical
testing of microblasted, acid-etched/microblasted, anodized, and discrete crystalline deposition surfaces. An experimental study in beagle dogs. Int J Oral Maxillofac Impl.MENTS 82, 114903 (2011)
Browaeys H, Vandeweghe S, Johansson CB, Jimbo R, Deschepper E, De Bruyn H. The histologic evaluation of osseointegration of Osseotite and Nanotite microimplants immediately loaded in conjunction with sinus lifting in humans. Clin Oral Implants Res, In Press.
Chvez de Paz LE, Lemos JA, Wickstrm Cand Sedgley CM. Role of (p)ppGpp in biofilm
formation by E. faecalis Appl Environ Microbiol 2011, Accepted for publication.
Chowdhary R, Jimbo R, Scharfe Thomsen C, Carlsson L, Wennerberg A. Biomechanical

evaluation of macro and micro designed screw-type implants: An insertion torque and removal torque study in rabbits. Clin Oral Implants Res, Accepted for publication.
Coelho PG, Granato R, Marin C, Jimbo R, Lin S, Witek L, Suzuki M, Bonfante EA. Effect of Si
Addition on Ca- and P- Impregnated Implant Surfaces with Nanometer Scale Roughness. An Experimental Study in Dogs. Clin Oral Impl Res; 2011, Available on early viewpublication.
Del Corso M, Vervelle A, Simonpieri A, Jimbo R, Inchingolo F, Sammartino G, Dohan Ehrenfest DM. Current knowledge and perspectives for the use of Platelet-Rich Plasma (PRP) and
44
Platelet-Rich Fibrin (PRF) in oral and maxillofacial surgery. Part 1: periodontal and dentoal veolar surgery. Current Pharmaceutical Biotechnology, Accepted for Publication.
Dohan Ehrenfest DM, Bielecki T, Jimbo R, Barb G, Marco Del Corso M, Inchingolo F,
Sammartino G. Do the fibrin architecture and leukocyte content influence the growth factor release of platelet concentrates? An evidence-based answer comparing a Pure Platelet-Rich Plasma (P-PRP) Gel and a Leukocyte- and Platelet-Rich Fibrin (L-PRF). Current Pharmaceutical Biotechnology, Accepted for Publication
Dorkhan M, Chvez de Paz LE, Skep M, Svensater G and Davies JR. (2012). Effects of saliva
or serum coating on adherence of Streptococcus oralis strains to titanium. Microbiol 158; 390-397.
Dunr P, To F, Bjrkbacka H, Fredrikson GN, Nilsson J, Bengtsson E. Immunization of apoE-/mice with aldehyde-modified fibronectin inhibits the development of atherosclerosis. Cardiovasc Res, 91(3):528-36, 2011.
Engelbertsen D, Anand DV, Fredrikson GN, Hopkins D, Corder R, Shah PK, Lahiri A, Nilsson
J, Bengtsson E. High levels of IgM against methylglyoxal-modified apolipoprotein B 100 is associated with less coronary artery calcification in patients with type 2 diabetes. J Int Med, in press. doi: 10.1111/j.1365-2796.2011.02411.x. [Epub ahead of print]
Falk M., Blum Z., Shleev S. Direct electron transfer based enzymatic fuel cells. Invited review.
Electrochimica Acta, 2012, Accepted for Publication
Falkman P, berg C, Clemens A, and Sparr E, Lyotropic lipid phases confined in cylindrical
pores: structure and permeability, J Phys Chem B 115(49), 14450-61, 2011
Fransson H, Petersson K, Davies JR. Dentine sialoprotein and collagen I expression after
experimental pulp capping in humans using emdogain gel. Int Endod J 2011, 44:259-267.
Frjd V, Chvez de Paz LC, Andersson M, Wennerberg A, Davies JR and Svenster G. In situ
analysis of multispecies biofilm formation on customized titanium surfaces. Mol Oral Microbiol, 2011, 26:241-252
Frjd V, Linderbck P, Wennerberg A, Chvez de Paz L, Svenster G and Davies JR. Effect of
nanoporous TiO2 coating and anodized Ca2+ modification of titanium surfaces on early microbial biofilm formation. BMC Oral Health. 2011 Mar 8;11:8. Gaidamauskas E., Rynkun P., Naz C., Gaigalas G., Jnsson P., and Godefroid M., Tensorial form and matrix elements of the relativistic nuclear recoil operator, Journal of Physics B, 44 175003 (2011).
Gonalves I, Stollenwerk M, Lindholm MW, Dias N, Pedro L, Fernandes e Fernandes J,

Moses J, Fredrikson GN, Nilsson J, Ares MPS. Activator Protein-1 in Carotid Plaques is related to Cerebrovascular Symptoms and Cholesteryl Ester Content. Cardiovasc Pathol, JanFeb;20(1):36-43, 2011.
Halgren C, Kjaergaard S, Bak M, Hansen C, El-Schich Z, Anderson C, Henriksen K, Hjalgrim

H, Kirchoff M, Bijlsma E, Nielsen M, den Hollander N, Ruivenkamp C, Isidor B, Le Caignec C, Zannolli R, Mucciolo M, Renieri A, Mari F, Anderlid BM, Andrieux J, Dieux A, Tommerup N, Bache I., Corpus callosum abnormalities, intellectual disability, speech impairment, and autism in patients with haploinsufficiency of ARID1B., Clin Genet. 2011 Jul 29. doi: 10.1111/j.1399-0004.2011.01755.x. [Epub ahead of print]
45
Halldin A, Jimbo R, Johansson CB, Wennerberg A, Jacobsson M, Albrektsson T, Hansson S. The effect of increased installation torque on implant stability. Bone 2011 Oct; 49(4): 783-9. Hejman U, Bjerken C, Dissolution driven crack branching in polycarbonate. FATIGUE & FRACTURE OF ENGINEERING MATERIALS & STRUCTURES Volume: 34 Issue: 4 Pages: 227-239
Henssge U, Do T, Gilbert SC, Clarke D, Wickstrm C, Ligtenberg AJM, Radford D and Beighton
D. Application of multi-locus sequence typing and pilus gene (fimA and fimP) sequence comparisons to investigate the population structure in Actinomyces naeslundii and Actinomyces oris. PLoS ONE, 2011 6:e21430. doi:10.1371/journal.pone.0021430.
Hirakawa Y, Jimbo R, Shibata Y, Watanabe I, Wennerberg A, Sawase T. Accelerated bone formation on photo-induced hydrophilic titanium implants: an experimental study in the dog mandible. Clin Oral Impl Res, In Press
Hu L.H., Farrokhnia M., Heimdal J., Shleev S., Rulisek L., Ryde U. Reorganization energy
for internal electron transfer in multicopper oxidases. Journal of Physical Chemistry B, 2011, 115(45), 13111-13126.
Ivan Alajbeg; Denise P Falco, Simon D Tran, Rafael A Martn-Granizo, Gloria I Lafaurie,
Gloria R Bautista Mendoza, Slavica Pejda, Lea Vuleti, Rubn Mantilla; Soraya C Leal, Ana C Barreto Bezerra, Henri A Mnard, Suguru Kimoto, Shaoxia Pan, Mara L Maniegas Lozano, Cheryl A Krushinski, Dario Melilli, Giuseppina Campisi, Carlo Paderni; Domenica Matranga, Juan F Yepes, Liselott Lindh, Meltem Koray, Gonca Mumcu, Sharon Elad, Itai Zeevi, Beatriz C Aldape, Rodrigo M Lpez Snchez, Claire L Lassauzay, Olivier Fromentin, Ben Z Beiski, Franz P Strietzel, Yrj T Konttinen, Andy Wolff, Susan L Zunt., Intraoral Electrostimulator for Xerostomia Relief: A Long-term, Multicenter, Open-label Clinical Trial. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology Accepted 2012-01-03.
J. Li, C. Dong, P. Jnsson, and G. Gaigalas, MF -dependent Hyperfine Induced Transition Rates
in an External Magnetic Field for Be-like 47Ti18 , Physics Letters A 375 914 (2011).
+
Jimbo R, Coelho PG, Vandeweghe S, Schwartz-Filho HO, Hayashi M, Ono D, Andersson M, Wennerberg A. Histological and three-dimensional evaluation of osseointegration to nanostructured calcium phosphate-coated implants. Acta Biomater, Accepted for Publication Jimbo R, Rodriguez-Fernandez J, Sul YT, Johansson CB. Principal component analysis: A novel analysis to evaluate the characteristics of osseointegration on different implant surfaces. Implant Dent, Accepted for Publication Jimbo R, Xue Y, Hayashi M, Schwartz HO, Andersson M, Mustafa K, Wennerberg A. Gene expression and biomechanical analysis of nano-structured calcium phosphate implants. J Dent Res, Accepted for publication Jimbo R., Sotres J., Johansson C., Breding K., Currie F., Wennerberg A. The biological response to three different nanostructures applied on smooth implant surfaces. 2011. Clinical Oral Implants Research. doi:10.1111/j.1600-0501.2011.02182.x Johansson CB, Gretzer C, Jimbo R, Mattisson I, Ahlberg E. Enhanced implant integration with hierarchically nano-structured implants; a pilot study in rabbits. Clin Oral Impl Res, Accepted for Publication Ketelhuth DFJ, Rios FJO, Wang Y, Liu H, Johansson ME, Fredrikson GN, Hedin U, Gidlund
46
M, Nilsson J, Hansson GK, Yan Z-Q. Identification of a danger-associated peptide from apolipoprotein B100 (ApoBDS-1) that triggers innate pro-atherogenic responses. Circulation, Nov 29;124(22):2433-43, 2011. Kocherbitov, V.; Alfredsson, V. Assessment of Porosities of SBA-15 and MCM-41 Using Water Sorption Calorimetry. Langmuir (2011), 27, 3889-3897.
Kolbus D, Wigren M, Ljungcrantz I, Sderberg I, Bjrkbacka H, Nilsson J and Fredrikson GN.

Immunization with cationized BSA inhibits progression of disease in Apobec-1/LDL receptor deficient mice with manifest atherosclerosis. Immunobiology, 216:663-669, 2011.
Lavant, E., Agardh, D., Nilsson, A., & Carlson, J. (2011). A new PCR-SSP method for HLA
DR-DQ risk assessment for celiac disease. Clinica chimica acta; international journal of clinical chemistry
Leisnert L, Karlsson M, Franklin I, Lindh L, Wretlind K., Improving teamwork between students
from two professional programmes in dental education. Eur J Dent Educ. 16: 17-26, 2012
Neilands J, Petersson LG, Beighton D and Svenster G. Fluoride-supplemented milk inhibits acid
tolerance in root surface biofilms. Caries Res 2011, Accepted for Publication
Neilands J, Sutherland D, Resin A, Wejse PL and Chvez de Paz LE. Chitosan nanoparticles
affect the acid tolerance response in adhered cells of Streptococcus mutans. Caries Res 2011, 45:501-505.
Ohlsson B, Sjberg K, Alm R, Fredrikson GN. Patients with irritable bowel syndrome (IBS)
and dysmotility express antibodies against gonadotropin realising hormone (GnRH) in serum. Neurogastroenterol Motil, Nov; 23(11):1000-6, e459, 2011.
Olof Svensson and Thomas Arnebrant, Antibody-Antigen interaction at polystyrene: An in

situ ellipsometric study, Journal of Colloid and Interface Science, in press 2011, doi: 10.1016/j. jcis.2011.11.034
Ono D, Jimbo R, Kawachi G, Ioku K, Ikeda T, Sawase T. Lateral bone augmentation with a
newly developed beta-tri-calcium phosphate block: an experimental study in the rabbit mandible. Clin Oral Impl Res; 2011, Available on early view
P. Idelicato, J. Bieron, and P. Jnsson, Are MCDF calculations 101 % correct in the superheavy
elements range?, Theoretical Chemistry Accounts: Theory, Computation, and Modeling (Theoretica Chimica Acta) 129, Numbers 3-5, 495 (2011).
P. Jnsson, P. Rynkun, and G. Gaigalas, Energies, Transition Rates, Hyperfine Structures, and
Land gJ Factors for the Fine-structure Levels of the 2s2 2p2, 2s2 p2, and 2p4 Configurations in Carbon-like Ions between F IV and Ni XXIII, Atomic Data and Nuclear Data Tables, 97 648 (2011).
Penkova, A.; Polotskaya, G.; Toikka, A.; Kocherbitov, V. Effect of residual solvent on physicochemical properties of poly(phenylene isophtalamide) membrane. Drying Technology (2011), 29, 633-641.
Pihl M, Davies JR, Johansson AC and Svenster G. Bacteria on catheters from patients undergoing peritoneal dialysis. Peritoneal Dialysis Int 2011, Accepted for Publication
47
Pita M., Gutierrez-Sanchez C., Olea D., Velez M., Garcia-Diego C., Shleev S., Fernandez V.M.,
De Lacey A.L. High redox potential cathode based on laccase covalently attached to gold electrode. Journal of Physical Chemistry C, 2011, 115(27), 13420-13428.
Reichhardt, N.; Nylander, T.; Klsgen, B.; Alfredsson, V.; Kocherbitov, V. Porosity and surface
properties of SBA-15 with grafted PNIPAAM: A water sorption calorimetry study. Langmuir (2011), 27, 1383813846.
Santos, O., Lindh, L., Halthur, T., Arnebrant, T., Adsorption from saliva to silica and hydroxyapatite surfaces and elution of salivary films by SDS and delmopinol. Biofouling virtual special issue 2011
Santos, O, Svendsen, IE, Lindh, L, Arnebrant, T, Adsorption of HSA, IgG and laminin-1 on
model titania surfaces effects of glow discharge treatment on competitively adsorbed film composition Biofouling, 27: 1003-1015, 2011
Senadheera DB, Cordova M, Ayala EA, Chvez de Paz LE, Singh K, Downey JS, Svenster G,
Goodman SD and Cvitcovitch D. Regulation of bacteriocin production and cell death by the VicRK signaling system in Streptococcus mutans. J. Bact 2011, Accepted for Publicatio
Simonpieri A, Del Corso M, Vervelle A, Jimbo R, Inchingolo F, Sammartino G, Dohan Ehrenfest

DM. Current knowledge and perspectives for the use of Platelet-Rich Plasma (PRP) and PlateletRich Fibrin (PRF) in oral and maxillofacial surgery. Part 2: bone graft, implant and reconstructive surgery. Current Pharmaceutical Biotechnology, Accepted for Publication
Singh R. N, Viswanathan U K, Kumar Sunil, et al. Influence of hydrogen content on impact

toughness of Zr-2.5Nb pressure tube alloy. NUCLEAR ENGINEERING AND DESIGN Volume: 241 Issue: 7 Pages: 2425-2436
Sonesson M, Ericson D, Kinnby B and Wickstrm C. Glycoprotein 340 and sialic acid in minorgland and whole saliva of children, adolescents and adults. Eur J Oral Sci 2011, 119:435-440.
Sonestedt E, Wirflt E, Wallstrm P, Gullberg B, Drake I, Hlebowicz J, Nordin Fredrikson G,

Hedblad B, Nilsson J, Krauss R, and Orhu-Melander M. High disaccharide intake associates with atherogenic lipoprotein. Br J Nutr, 2011 Oct 20:1-8. [Epub ahead of print]
Sotres J., Barrantes A., Arnebrant T. Friction Force Spectroscopy as a Tool to Study the Strength
and Lateral Diffusion of Protein Layers. 2011. Langmuir. 27: 9439-9448
Sotres J., Svensson O., Arnebrant T. Friction force spectroscopy of - and -casein monolayers.
2011. Langmuir. 27: 981-992.
Sotres, J, Lindh, L, Arnebrant, T, Friction and force measurements on HWS and influence of pH
changes on film stability. Langmuir, 27:13692-700, 2011, doi:10.1021/la202870c
Stollenwerk M, Svensson O, Schiopu A, Jansson B, Arnebrant T, and Fredrikson GN. Adsorption

of low density lipoprotein, its oxidation and subsequent binding of specific recombinant antibodies an in situ ellipsometric study. Biochimica et Biophysica Acta, Feb;1810(2):211-7, 2011.
Strietzel FP, Lafaurie GI, Bautista Mendoza GR, Alajbeg I, Pejda S, Vuleti L, Mantilla R, Falco
DP, Leal SC, Barreto Bezerra AC, Tran SD, Mnard HA, Kimoto S, Pan S, Martn-Granizo RA, Maniegas Lozano ML, Zunt SL, Krushinski CA, Melilli D, Campisi G, Paderni C, Dolce S, Yepes JF, Lindh L, Koray M, Mumcu G, Elad S, Zeevi I, Aldape Barrios BC, Lpez Snchez RM, Beiski BZ, Wolff A, Konttinen YT. Efficacy and safety of an intraoral electrostimulation device for
48
xerostomia relief: a multicenter randomized trial. Arthritis & Reumatism. 63: 180-190, 2011, DOI: 10.1002/art.27766
Sumi T, Braian M, Shimada A, Shibata N, Takeshita K, Vandeweghe S, Coelho PG, Wennerberg

A, Jimbo R. Characteristics of implant-CAD/CAM abutment connections of 2 different internal connection systems. J Oral Rehab, Accepted for Publication
Svendsen, IE, Santos, O, Sotres, J, Breding, K, Wennerberg, A, Arnebrant, T, Lindh, L, Adsorption

of HSA, IgG and laminin-1 on model hydroxyapatite surfaces effects of surface characteristics Biofouling, 28: 87-97, 2012
Svendsen, IE., Lindh, L. The composition of enamel salivary films is different from the ones
formed on dental materials. Biofouling virtual special issue 2011
Valverde GB, Jimbo R, Teixeira HS, Bonfante EA, Janal MJ, Coelho PG. Evaluation of surface
roughness as a function of multiple blasting processing variables. Clin Oral Implants Res, In Press
Wang X., Falk M., Ortiz R., Matsumura H., Bobacka J., Ludwig R., Bergelin M., Gorton L.,
Shleev S. Mediatorless sugar/oxygen enzymatic fuel cells based on gold nanoparticle-modified electrodes. Biosensors and Bioelectronics, 2012, 31(1), 219-225.
Wennerberg A, Jimbo R, Allard S, Skarner G, Andersson M. In vivo Stability of hydroxyapatite

nano-meter particles coated on titanium implant surfaces. Int J Oral Maxillofac Implants. 2011 Nov-Dec; 26(6): 1161-6
Vidakovic-Koch, T, Ivanov I, Falk M, Shleev S, Ruzgas T, Sundmacher K, Impact of the gold

support on the electrocatalytic oxidation of sugars at enzyme-modified electrodes. Electroanalysis, 2011, 23(4), 927-930.
Wigren M, Kolbus D, Dunr P, Ljungcrantz I, Sderberg I, Bjrkbacka H, Fredrikson GN,

Nilsson J. Evidence for a role of regulatory T cells in mediating the athero-protective effect of apolipoprotein B peptide vaccine. J Int Med, May 269(5):546-56, 2011.
BOOKS AND BOOK CHAPTERS

Albrektsson T, Wennerberg A. The science of osseointegration. In; Prosthodontic Treatment for
Edentulous Patients, ed 13. Submitted.
2.
Alm K, Cirenajwis H, Gisselsson L, Gjrloff Wingren A, Janicke B, Mlder A, Oredsson S,

Persson J. Digital holography and cell studies. Holography, Research and Technologies, In Tech, edited by Joseph Rosen February 2011. ISBN 978-953-307-227-2
Blum, Z. and Eriksson, H. Dealuminated Zeolites in Biological Systems, in; Zeolites: Synthesis,
Chemistry and Applications. Editors: M. K. Andreyev and O. L. Zubkov, Nova Science Publishers, Inc., N Y. In press 4.
Davies, J.R., Wickstrm, C. and Thornton, D. J. Gel-forming and cell-associated mucins preparation for structural and functional studies. In: Mucins: Methods and Protocols, Methods in Molecular Biology, vol. 842, Eds: McGuckin MA and Thornton DJ DOI 10.1007/978-1-61779513-8_2, Springer Science+Business Media, LLC. Accepted for Publication
Gotfredsen K, Wennerberg A, Gunne J. Implants and Their Role in FDP Treatment. Fixed Prosthodontics eds Nilner, Karlsson, Dahl. Submitted
49
Jimbo R. Abutment selection, screw or cementation? Prosthetic Practice, 2011 (Japanese) Johansson CB, Jimbo R, Rosen K. Histological analysis. Comprehensive biomaterials. Elsevier
2011
Ono D, Jimbo R, Sawase T. Impression techniques in implant prosthetics. Prosthetic Practice,

2011 (Japanese)
Wennerberg A, Rosn B-G, Albrektsson T. Surface topography and measuring techniques for
dental implant applications. -Possibilities and obstacles In Press Implant Dentistry Research Guide: Basic, Translational and Experimental Clinical Research. Nova Science Publishers
Winning T, Skinner V, Kinnell A, Townsend G, Svenster G, Rohlin M and Davies J. The Influence of Two PBL Curricula Contexts on First-year Students Understandings of PBL, Approaches to Learning and Outcomes; In: Problem-Based Learning in Clinical Education. Eds: Bridges S; McGrath C and Whitehill, T L. Springer Science + Business Media LLC, Accepted for Publication
3. POPULAR ARTICLES
F Hasse, N Tibbelin and JR Davies. Protease activity in Lactobacillus salivarius and Actinomyces naeslundii in a model of root canal infection. Swed Dental J 2011, 35:226.
H Fransson, K Petersson and JR Davies. Influence of bacterial products on the viability of

odontoblastlike cells. Swed Dental J 2011, 35:227.
Wennerberg A. Em Foco, Entrvista. Jornal Ilapedo 2011 vol 5, no 3. Brazil.
50
VISITORS AND SEMINARS AT THE CENTER

Prof. Lars Wads, Lund Univeristy, Sorption calorimetry as a tool to study hydration, 2011-04-01 Gediminas Niaura, Vibrational spectroscopy for hydration studies, 2011-04-01 Audrius Misiunas, Jekaterina Latynis, Spectroscopic studies of hydration of lysozyme and cytochrome c, 2011-04-01
Dr. Justas Barauskas, Hydration of lipid-based drug delivery systems, 2011-04-01 Prof. Hkan Wennerstrm Interlamellar forces and the thermodynamic characterization of
lamellar phospholipid systems, 2011-04-01
Prof. Alexander Toikka, Department of Chemical Thermodynamics and Kinetics, Saint-Petersburg University, Application of thermodynamic methods to the membrane processes: description and modeling of pervaporation, 2011-04-01
Prof. Irina Zvereva, Department of Chemical Thermodynamics and Kinetics, Saint-Petersburg

University, Processes of hydration and cation exchange in layered oxide structures, 2011-04-01
Sergei Gavrushov, Dielectric model of hydration shells of metal ions: an impact on electrostatics
of DNA/RNA, 2011-04-01
Prof. Gyrgy Marko-Varga, Dept. of Measurement Technology and Industrial Electrical Engineering , Lund University, Clinical Analysis in Lung Cancer and COPD Studies, 2011-06-14
Dr. Javier Sotres, Health and Society, Malm University, Friction Force Spectroscopy of Protein
Layers: Strength and Lateral Diffusion Studies, 2011-06-14
Dr. Maria Pihl, Institutionen fr kemi- och bioteknik, Chalmers, Microbial biofilms on peritoneal dialysis catheters, 2011-06-14
Prof. Erik V Thomsen, DTU Nanotech, Technical University of Denmark, Sensors Everywhere:
An Electronic Patch, 2011-10-24
Malin Prydz, Product Designer, Mlnlycke Health Care AB, Wound Dressings - From Product
Design Perspective, 2011-10-24
Dr. Maria Wertn, Mlnlycke Health Care AB, In Vitro Testing of Bioactive Wound Dressings
During Product Development, 2011-10-25
John McCormack, MiniFAB, Nanolitre Tear Sampling in Diagnostics: Concept to Commercial

Product, 2011-10-25
Assoc. Prof. Sergey Shleev, Health and Society, Malm University, Operation of Microscale
Electrochemical (Bio)Devices in Human Lacrimal Liquid (Tears) , 2011-10-25
Prof. Enno Veerman, Department Oral Biochemistry, Academic Centre Dentistry Amsterdam,
Saliva-based Diagnostics: A Critical Appraisa, 2011-10-25
Dr. Javier Sotres, Health and Society, Malm University, Friction Force Spectroscopy as a Tool
to Study Salivary Films, 2011-10-25
Prof . Thomas Arnebrant, Health and Society, Malm University, Interfacial Behavior of Salivary
Molecules Relevance to Diagnostics, Sensing and Device Construction, 2011-10-25
51
Assoc. Prof. Marie Lodn and Ulf kerstrm, Skin barrier function and humectants in moisturizers, 2011-11-15
Dr. Per Mercke, Immaterial property rights, 2011-12-08 Laura Adamonyte, Master student, Vilnius University, 2 months. Phase studies of synthetic lipids
(Erasmus scholarship)
Marija Jankunec, PhD student, Vilnuis University, 3 months. Studies of hydration and phase
behavior of lipids. Title of presentation: Hydration of lipid systems. DOPC, 2011-01-18. (Swedish institute Visby program)
Jekaterina Latynis, PhD student, Vilnuis University, 3 months. Studies of hydration of proteins.
Title or presentation: Spectroscopic studies of hydration of lysozyme and cytochrome c, 2011-04.01. (Swedish institute Visby program)
Anastasia Penkova, Post-doc, St.Petersburg University, 1 month. Studies of diffusion of model

compounds in biopolimers. (Swedish institute Visby program)
Mikhail Chislov, PhD student, St.Petersburg University, 1 month. Experiments on hydration of

layered oxides. (Swedish institute Visby program)
Anastasiia Venediktova, PhD student, St.Petersburg University, 1 month. Experimental studies

of phase behavior of surfactants. (Swedish institute Visby program)
Dr. Karin Bergstrm, AkzoNobel, Sweden, 2011-02-14 15 Dr. Christy Whiddon, AkzoNobel, Sweden, 2011-02-14 15 Dr. Peter Westbye, AkzoNobel, Sweden, 2011-02-14 15 Prof. Peter Bauer, Bayer Crop Science, Germany, 2011-02-14 15 Dr. Shuaib A Khan, AkzoNobel, NY, USA, 2011-02-14 15 Dr. Dmitrii Pankratov, a guest researcher from Institute of Biochemistry, Russian Academy of
Sciences, Moscow, Russia, 2011-07-13 09-09
Mr. Paul Santner, Master student of Biotechnology from BOKU University, Vienna, Austria,
2011-09-12 12-30
Marius Dagys, PhD at Vilnius University, Vilnius, Lithuania. 2011-04-04 05-27. Vida Krikstolaityte, PhD at Vilnius University, Vilnius, Lithuania. 2011-09-01 2012-08-31 Laura Abariute, Erasmus supported master student, Vilnius Gediminas Technical University,
Vilnius, Lithuania. 2011-06-01 08-30
Tetsuro Odatsu, 2 months Daisuke Ono, 10 days Kenji Miyahara, 10 days Ryota Muratomi, 10 days Koutaru Tsuiki, 10 days Kazuhiro Nakashima, 10 days Humberto Scwartz, 3 months Anders Zachrisson
52
Prof. Jeannine Brady, University of Florida, USA Prof. Gunnar Bergenholtz, University of Gothenburg Assoc. Prof. Anette Carln, Gothenburg University Assoc. Prof. Marie Skep, Lund University Assoc. Prof. Tim Tolker-Nielsen, University of Copenhagen Dr. Peter Weise, ArlaFoods Dr. Jan Hall, Nobel Biocare Dr. Helena Jeppsson, Gambro Dr. Anna Westerlund, Gothenburg University Dr. Torgny Sjdin, Sinclair Pharma Anthony P F Turner, Center for Biosensors and Bioelectronics, Linkping University, Biosensors: meeting the demand for personal diagnostics, 2011-02
INTERNAL SEMINARS AT THE CENTER
Debby Chang , Department of Physical Chemistry, Lund University, Lubrication of biological

joints, 2011-02
Sven Scholander, STP Kristiandstad, Singel implantats, 2011-02 Stefan Vandeweghe, Prosthetic Dentistry, Mah, Factors for implant survival , 2011-03 Rolf Liedholm, Oral Surgery, and Liselott Lindh, Prosthetic Dentistry, Mah, Dry mouth signs
and treatments , 2011-03
Yvone Ceder, Department of Clinical Chemistry, Lund University, MicroRNAs in prostate

cancer, 2011-03
Henrietta Nielsen, Dept of Clinical Sci Malm, Molecular Memory Research Unit, The Wallenberg Lab, Skne University Hospital Malm, 2011-03
Maria Pihl, Mah, Biofilms on peritoneal dialysis catheters., 2011-03 Marjan Dorkhan Mah, Biofilms on titanium implant surfaces, 2011-03 Ingrid Collin-Bagewitz, STP Malm and Kerstin Petersson, Endodontics, Mah, National guidelines in dentistry , 2011-03
Anders Sundh, Ume University, Zirconia in odontology , 2011-04 Eddie Thordarson, Magle Life Science AB, Kristianstad/Lund, Biodegradable microspheres as
medical devices, 2011-04
Maria Stollenwerk, Biomedical Laboratory Science / Technology, Malm University, Adsorption of low-density lipoprotein, its oxidation, and subsequent binding of specific recombinant antibodies: An in situ ellipsometric study., 2011-05
Henrietta Nielsen, Dept of Clinical Sci Malm, Molecular Memory Research Unit, The Wallenberg Lab, Skne University Hospital Malm, The glial spect of neurodegenerative dementia, 2011-05
Olle Melander, Lund University Diabetes Centre & Skne University Hospital in Malm,
Biomarkers of cardiovascular diseases 2011-06
53
Liv Kroona, Mah Lichenoid changes and Oral Biofilms, 2011-06 Lars Bondemark Nursing Research - Status and Future, 2011-09 Aje Carlbom, HS Qualitative methods - a general overview , 2011-10 Magnus Englander, HS Phenomenological Research Method, 2011-10 Andreas Pettersson & Krister Nilner, Prosthetic dentistry, Mah, CAD/CAM-guided dental
implant surgery , 2011-10
Magnus Falk, Mah, Design and performance of biofuel cells, 2011-11 Peter Billsten, Mah, Pharmaceutical applications of Raman spectroscopy and imaging,
2011-11
Mats Trulsson, KI, Stockholm, Oral motor activity, 2011-11 Maria Alvarado-Kristensson, Department of Pathology, Lund University, Malm, Regulators
of the restriction point and cancer, 2011-12
Cathrine Albr, Mah, Penetration of model drug compounds through skin, 2011-12
54
CONTRIBUTIONS TO AND PARTICIPATION IN CONFERENCES AND WORKSHOPS

ORAL PRESENTATIONS 2011
Bjrklund S, A water gradient can be used to regulate biophysical properties of skin, 2nd Skin
Trail Blazer Workshop, Embassy Suites Boston Massachusetts Institute of Technology (USA) 5-6 Aug, 2011
1.
INTERNATIONAL
Davies JR and Bondemark L. Research Education at The Faculty of Odontology, Malm

University. University of Copenhagen, 2011. Invited speaker.
Dorkhan M, Chavz de Paz, Svenster G and Davies JR. Effect of saliva and serum pellicles on
adhesion of Streptococcus oralis to titanium surfaces. 10th European Oral Microbiology Workshop, Zeist, The Netherlands, 2011.
Jimbo R. Analysing modern implants and its clinical significance. Osseospeed Introductory
meeting, 2011
Jimbo R. Effect of nanostructures on osseointegration and new evaluation approaches. Nanotechnology in Odontology, 2011
Jimbo R. State of the art implant research topics and future paths, 2011 Neilands J, Davies JR and Svenster G. The effect of Lactobacillus strains on Streptococcus
mutans acid adaptation. 10th European Oral Microbiology Workshop, Zeist, The Netherlands, 2011
P. Jnsson, S. Gustafsson, J. Ekman, P. Bengtsson, P. Rynkun, and G. Gaigalas, CI calculations,

43st European Group for Atomic Systems, Fribourg (Switzerland), 28 Jun 2 Jul, 2011
Peter Lamberg, Marius Dagys, Javier Sotres, Thomas Arnebrant and Tautgirdas Ruzgas,
Comparison of techniques for determination of nanoparticle diameter. 5th SMCBS2011 Workshop at ochw, Poland, 4-8 Apr, 2011
S. Verdebout, J.G. Li, C. Naze, M. Godefroid, P. Jnsson, and G. Gaigalas, Atomic properties of
neutral Ne using the LCFI method, 43st European Group for Atomic Systems, Fribourg (Switzerland), 28 Jun 2 Jul, 2011
S. Verdebout, M. Godefroid, P. Rynkun, P. Jnsson, G. Gaigalas, and C. Froese Fischer, Interaction of Variational Localized Correlation Functions for Atomic Properties, 43st European Group for Atomic Systems, Fribourg (Switzerland), 28 Jun 2 Jul, 2011
S. Verdebout, P. Rynkun, P. Jnsson, G. Gaigalas, C. Froese Fischer and M. Godefroid, Interaction of Variational Localized Correlation Functions for Atomic Properties of Be I, XXVII International Conference on Photonic, Electronic and Atomic Collisions Belfast, (Northern Ireland, UK), 27 Jul 2 Aug, 2011
55
Schwartz-Filho HO, Wennerberg A, Jimbo R, Ramos-Junior ES, Morandini ACF, Marcantonio

RAC, Marcantonio E Jr, Santos CF. Cytokine production in gingival fibroblasts cultured on distinct titanium surfaces. Latin American Osseointegration Congress, 2011
Shleev S., Invited Lecture entitled: Mediatorless sugar/oxygen enzymatic fuel cells based on
gold nanoparticle-modified electrodes at the 62st Annual Meeting of ISE, Niigata, Japan
Shleev S., Invited Lecture entitled: Miniaturized enzymatic fuel cells at 2011 Workshop on
Biomimetic and Bioanalytical Systems, Luckenwalde, Germany
Shleev S., Invited Lecture entitled: Nanostructured surfaces for biofuel cell application at 5th
Nanoschool, Vilnius, Lithuania
Shleev S., Invited Lecture entitled: Nanostructured surfaces for biofuel cells at Creative Nano,
Taastrup, Denmark
Shleev S., Invited Lecture entitled: Potentially implantable bioelectronic devices for biosensing
and biofuel cell applications at SMCBS2011 International Workshop, Lochw, Poland
Shleev S., Invited Lecture entitled: Three-dimensional nanobiostructure-based self-contained

biodevicesfor biomedical application at Update 2011, Helsingborg, Sweden
Svenster G. Control of Bacterial Function in Relation to Disease. 2nd European Congress on

Microbial Biofilms, Copenhagen, Denmark, 2011. Invited speaker.
Svenster G. Infection control on the biofilm level. Scandinavian Society for Biomaterials 4th
Annual Meeting, Fiskebckskil, Sweden, 2011. Invited speaker.
Wennerberg A. Biological and mechanical responses to implants, abutments and supraconstructions. 2011 International conference, Southern Implants, 9-11 May 2011 Chobe, Botswana. Invited lecture.
Wennerberg A. Finding optimal solutions for implant surfaces. Seminars of Turku Clinical
Biomaterial Center TCBC, Turku, Finland, 1 Mar 2011. Invited lecture.
Wennerberg A. Implant development. Past, present and future of implant surfaces. The 8th
Annual MEGAGEN international symposium. Milan Italy, 11-12 Nov, 2011. Invited lecture.
Wennerberg A. Implant surfaces and the importance for soft and bone tissue integration, 4
hour international course, In 2011, Latin American Osseointegration Congress, 2-5 July 2011. Invited lecture.
Wennerberg A. Implant surfaces, In 2011, Latin American Osseointegration Congress, 2-5 July
2011. Invited lecture.
Wennerberg A. Nanostructures for tissue adhesion. Pre conference. P-I Implants. In 2011, Latin
American Osseointegration Congress, 2-5 July 2011. Invited lecture.
Wennerberg A. Role of surface characterisation and improving osseointegration and related

advancement. The Indian Society of Oral Implantologists. 21-23 Oct 2011, Hyderabad, Indien. Invited lecture.
Wickstrm C, Chvez de Paz LE, Kindblom CA, Jonsson S and Svenster G. Using salivary
mucin MUC5B as a surface-associated conditioning film for bacterial studies. 9th European Symposium on Saliva, Egmond aan Zee, Netherlands, 2011. Invited speaker.
56
Fransson H, Petersson K and Davies JR. Influence of bacterial products on the viability of
odontoblastlike cells. 47th Annual Congress of the Swedish Society, Stockholm, 2011
NATIONAL
Hasse F, Tibbelin N and Davies JR. Protease activity in Lactobacillus salivarius and Actinomyces naeslundii in a model of root canal infection. 47th Annual Congress of the Swedish Society, Stockholm, 2011
Lindh L., Oral hygiene and dry mouth, Mun- & Halscancerfrbundets rehabiliteringskurs,
Hr, 2011
Svenster G, Neilands J, Wickstrm C, Kinnby B and Davies JR. Infection Control & Biomarkers on the biofilm level. Imego, Gothenburg, 2011
Svenster G, Neilands J, Wickstrm C, Kinnby B and Davies JR. Infection Control & Biomarkers on the biofilm level. Brnemark Clinic, Gothenburg, 2011
Dorkhan M. Activities of microbial biofilms on bioactive implant surfaces. PhD student workshop, Faculty of Odontology Malm University, 2011
LOCAL
Javier Sotres, Health and Society, Malm University, Friction Force Spectroscopy as a Tool to
Study Salivary Films, 2011-10-25
Javier Sotres, Health and Society, Malm University, Friction Force Spectroscopy of Protein
Layers: Strength and Lateral Diffusion Studies, 2011-06-14
Johan Engblom, Health and Society, Malm University, Hydration dependence of barrier function of biomembranes, 2011-04-01
Johan Engblom, Health and Society, Malm University, Responding Biological Membranes Skin and Mucosa, 2011-10-25
Maria Pihl, Institutionen fr kemi- och bioteknik, Chalmers, Microbial biofilms on peritoneal
dialysis catheters, 2011-06-14
Sergey Shleev, Health and Society, Malm University, Operation of Microscale Electrochemical
(Bio)Devices in Human Lacrimal Liquid (Tears) , 2011-10-25
Tautgirdas Ruzgas, Health and Society, Malm University, Characterisation of Biological

Membranes by Impedance Spectroscopy, 2011-10-24
Thomas Arnebrant, Health and Society, Malm University, Interfacial Behavior of Salivary
Molecules Relevance to Diagnostics, Sensing and Device Construction, 2011-10-25
Vitaly Kocherbitov, Health and Society, Malm University, Driving forces of hydration of
surfactants, 2011-04-01
Vitaly Kocherbitov, Health and Society, Malm University, Thermodynamics of hydration of

proteins, 2011-04-01
57
2. POSTERS 2011
INTERNATIONAL Ahlgren C, Bjrnsson L, Diogo-Lfgren C, Fransson, H, Jansson H, Johansson V, Lager A,
Ljunggren A, Neilands J, Papias E, Petrn S, Sonesson M and Wickstrm C. The Malm Model: How to Achieve Future Sustainability in a Problem-Based Curriculum. 7th International Conference on PBL in Dentistry, Whistler, Canada, 2011
Bjrklund, S, A water gradient can be used to regulate biophysical properties of skin, Barrier
Function of Mammalian Skin, Waterville Valley Resort in Waterville Valley NH (USA), 7-12 Aug, 2011
Bougas K, Franke-Stenport V, Currie F, Wennerberg A. Laminin is highly reactive with titanium

surfaces in vitro. IADR/AADR/CADR San Diego, Calif., USA, 16-19March, 2011
Chvez de Paz LE, Svenster G and Wickstrm C. Compositional changes during biofilm
formation on human salivary MUC5B conditioning film. 2nd European Congress on Microbial Biofilms, Copenhagen, Denmark, 2011
Davies JR, Rohlin M and Svenster G. The Oral Ecosystem as a Model for Dental Education
and Research. 7th International Conference on PBL in Dentistry, Whistler, Canada, 2011
Dorkhan M, Chavez de Paz LE, Svenster G and Davies JR. Effect of surface roughness on
adhesion of Streptococcus oralis to titanium surfaces. 2nd European Congress on Microbial Biofilms, Copenhagen, Denmark, 2011
Falkman P, Pedersen L, Engblom J, Cubic phases in the DOPS/POPE/water system;Geometry at

Interfaces, Primosten (Croatia), 3-7 Oct, 2011
I.E. Svendsen, T. Arnebrant, L. Lindh, Effect of low pH rinsing on in situ formed salivary films.
9th European Symposium on Saliva. Saliva Symposium. 22-25 May, 2011 Egmond aan Zee, the Netherlands
Jimbo R, Coelho PG, Schwartz-Filho HO, Xue Y, Mustafa K, Andersson M, Wennerberg A.

Osseointegration enhanced by nano-structured CaP coated implants: a molecular, biomechanical and CT analysis. European Association for Osseointegration, 2011
Neilands J, Sutherland D, Resin A, Weise PL, Chavez de Paz LE. Chitosan nanoparticles affect
the acid tolerance response in Streptococcus mutans biofilms. 2nd European Congress on Microbial Biofilms, Copenhagen, Denmark, 2011
Pihl M, Svenster G and Davies JR. Effects of extracellular products from Pseudomonas
aeruginosa on Staphylococcus epidermidis biofilms on peritoneal dialysis catheters. 2nd European Congress on Microbial Biofilms, Copenhagen, Denmark, 2011
Svensson B, Nieto C, Delfani P, Chia J, Gjrloff Wingren A, Ruzgas T, Engblom J, A modern

hydrogen peroxide cream for wound healing, AAD, New Orleans (USA) 2011
Svensson, B, Wingren, AG, Abad, CN, Chia, J, Delfani, P, A modern hydrogen peroxide cream
for wound healing, 69th Annual Meeting of the American Academy of Dermatology, New Orleans, LA (USA) 4-8 Feb, 2011
V. Krikstolaityte, A. Barrantes, T. Arnebrant, A. Ramanavicius, T. Ruzgas. Influence of ionic

strength on physicochemical properties of gold nanoparticle multilayer structures. 13th International conference-school on Advanced materials and technologies, 27-31 August, 2011, Palanga, Lithuania
Winning TA, Kinella A, Skinner V, Townsend G, Svenster G, Rohlin M, Davies JR. First- and
third-year students approaches to learning in two PBL dental curricula. 7th International
58
Conference on PBL in Dentistry, Whistler, Canada, 2011
Winning TA, Skinner V, Townsend G, Svenster G, Rohlin M, Davies JR. The First- and thirdyear students understandings of PBL from two dental curricula. 7th International Conference on PBL in Dentistry, Whistler, Canada, 2011
Albr C, Lodn M, Kocherbitov V, The effect of topical hyaluronic acid on skin permeability
and moisturization, Soft and Hard Materials, Lund (Sweden), 25-27 Oct, 2011
NATIONAL
Dorkhan M, Svenster G and Davies JR. Adhesion of Streptococcus oralis to titanium

surfaces- effects of surface roughness and conditioning films. Workshop on development and regeneration of bones and teeth. Ume (Sweden), 2011
Albr C, Lodn M, Kocherbitov V, The effect of topical hyaluronic acid on skin permeability and moisturization, Soft and Hard Materials, Lund (Sweden), 25-27 Oct, 2011 Alejandro Barrantes, Olga Santos and Thomas Arnebrant, Viscoelastic Properties of Poly-L Lysine/Heparin Multilayers, Biofilms 7th Annual workshop, Malm, 24-25 Oct, 2011 Bjrklund, S, A water gradient can be used to regulate biophysical properties of skin, Biofilms 7th Annual workshop, Malm, 24-25 Oct, 2011 El-Schich, Z, Mlder, A, Tassidis, H, Sebesta, M, Lenart, T, Gustafsson, M , Gisselsson, L, Alm, K, Hrknen, P, Gjrloff Wingren A, Using digital holography for measurements of growth, viability and death of adherent cells, Biofilms 7th Annual workshop, Malm, 24-25 Oct, 2011 Falkman P, Pedersen L, Engblom J, Cubic phases in the DOPS/POPE/water system, Biofilms 7th Annual workshop, Malm, 24-25 Oct, 2011 I.E. Svendsen, T. Arnebrant and L. Lindh, Effect of low pH rinsing on in situ formed salivary films, Biofilms 7th Annual workshop, Malm, 24-25 Oct, 2011 Javier Sotres, Alejandro Barrantes, Olof Svensson, Liselott Lindh, and Thomas Arnebrant, Friction Force Spectroscopy as a Tool to Study the Strength, Lateral Diffusion and Structure of Proteinaceous Layers, Biofilms 7th Annual workshop, Malm, 24-25 Oct, 2011 Jekaterina Latynis, Justas Barauskas and Gediminas Niaura, Hydration of Cytochrome C studied by vibrational spectroscopy and calorimetry, Biofilms 7th Annual workshop, Malm, 24-25 Oct, 2011
LOCAL
Laura Adamonyt, Justas Barauskas, Daiva Taurait, Behavior of Amide Derivatives of

Monooleoyl and Monolinoleoyl Glycerol Synthesis and Aggregation, Biofilms 7th Annual workshop, Malm, 24-25 Oct, 2011 M Dorkhan, L Chavez de Paz, G Svenster and JR Davies, Effect of surface roughness on adhesion of Streptococcus oralis to titanium surfaces, Biofilms 7th Annual workshop, Malm, 24-25 Oct, 2011
Magnus Falk, Viktor Andoralov, Zoltan Blum, Javier Sotres, Dmitry Suyatin, Tautgirdas Ruzgas,
Thomas Arnebrant, and Sergey Shleev, , Biofilms 7th Annual workshop, Malm, 24-25 Oct, 2011 Marija Jankunec, Justas Barauskas, Markus Johnsson and Vitaly Kocherbitov, The aqueous phase behavior of dioleoylphosphatidylcholine, Biofilms 7th Annual workshop, Malm, 24-25 Oct, 2011
59
V. Krikstolaityte, A. Barrantes, J.Sotres, T. Arnebrant, A. Ramanavicius1, T. Ruzgas,

Physicochemical properties of gold nanoparticle multilayers, Biofilms 7th Annual workshop, Malm, October 24-25, 2011
Znamenskaya Y, Engblom J, Arnebrant T, Kocherbitov V,Effect of Hydration on Thermodynamic

Properties of Pig Gastric Mucin, Biofilms 7th Annual workshop, Malm, 24-25 Oct, 2011
BSc AND MSc EDUCATION

1. BSc-LEVEL
Biomedical laboratory science (HS) Biomedical Technology TELMah (TS & HS) Dentistry program (OD)
2. MSc-LEVEL
Biomedical Methods and Technology (HS) M aterials science (TS & HS) Dentistry program (OD)
OTHER ACTIVITIES BY CENTER MEMBERS

Ann Wennerberg has been opponent for PhD theses at the University of Ghent, Belgium, and Gunilla Nordin Fredrikson has been opponent at a half-time control at the Department of Laboratory Medicine, Lund University. Tautgirdas Ruzgas, Gunilla Nordin Fredrikson, Hkan Eriksson, Anette Gjrloff Wingren, Johan Engblom, Thomas Arnebrant. Ann Wennerberg, Ryo Jimbo, Liselott Lindh, Claes Wickstrm, Julia Davies, Gunnel Svenster, and Per Sthle were members of examination committees for PhD
60
defences on 21 occasions during 2011. Ann Wennerberg has been an external reviewer for a promotion to professor at Ume University. Gunnel Svenster has been an expert reviewer for an application for promotion at the Karolinska Institute. Center members have been referees for international journals on a regular basis. The list of journals includes; Acta Biomaterialia, Acta Odontologica Scandinavica, Atherosclerosis, Archives of Oral Biology, Biofouling,
Biomaterials, Biomacromolecules, Bioelectrochemistry, Cancer Letters, Caries Research, Cellular Immunology, Chemistry and Physics of Lipids, Circulation, Clinical Chemistry, Clinical Oral Implants Research, Clinical Implant Dentistry and Related Research, Dental Materials Journal, Electroanalysis, Electrochemistry Communications, European Journal of Dental Education, European Journal of Oral Sciences, Experimental Lung Research, Human Immunology, Experimental Biological Medicine, International Journal of Dentistry, International Journal of Fracture, International J. Nanomedicine, International Journal of Oral and Maxillofacial Research, International Journal of Prosthodontics, International Journal of Solids and Structures, Journal of Anatomy, Journal of Applied Mechanics, Journal of Inorganic Biochemistry, Journal of Artificial Organs, Journal of Biomedical Materials Research, Journal of Biomedical Materials Research: Part B, Journal of Colloid and Interface Science, Journal of Dental Research, J Mechanical Engineering, Journal of Medical Microbiology, Journal of Oral Rehabilitation, Journal of Periodontal Research, Journal of Prosthodontic Research, Journal of Royal Society Interface, Journal of Periodontal Research, Langmuir, Oral Oncology, PLoS ONE, Physical Review B, Process Biochemistry, Protein and Peptide Letters, Proteome Science, Stroke, Vitae, Vaccine, Thrombosis and Haemostasis. Tautgirdas Ruzgas is on the editorial board for Nonlinear Analysis: Modelling and Control, and Per Sthle is on the editorial board for the International Journal of Fracture. Ann Wennerberg is on the editorial board for Acta
Biomaterialia, International Journal of Prosthodontics, and Acta Biomaterialia. Ryo Jimbo is on the editorial board for Clinical Implant Dentistry & Related Research, and Journal of Oral and Maxillofacial Research. Ann Wennerberg was co-ordinator, and Gunnel Svenster was the local co-ordinator at Malm University, of the National Graduate Research School in Odontology. Gunilla Nordin Fredrikson was examiner of papers for the degree of Bachelor of Science at Health and Society, Malm University. Thomas Arnebrant was examiner of the master s theses in the programme Biomedical Methods and Technology, Health and Society, Malm University, and on the Faculty Board, the Board for Research and Research Education at the Faculty of Health and Society, Malm University. Anette Gjrloff Wingren was chair of the Education Board at the Faculty of Health and Society (Malm University) as well as chair of the Board for Constitution of New Programmes (Malm University). Gunnel Svenster was chair of the Malm University Associate Professor Board as well as a member of the Research Committee, Faculty of Odontology, Malm University. Julia Davies was Director of Postgraduate Studies as well as co-ordinator for Basic Biology in Undergraduate Curriculum and a member of the Research Committee, Faculty of Odontology, Malm University. Tautgirdas Ruzgas was on the evaluation board for the appointment of associate professors at Malm University. Johan Engblom was a member of the Faculty Board at the School of Technology, Malm University, and the Education Board (UN) at the Faculty of Health and Society, Malm University. Claes
61
Wickstrm was Chairman of the Curriculum Committee and a member of the Education Committee, Faculty of Odontology, Malm University. Claes Wickstrm and Jessica Neilands were members of the Staff Development and Education Group, Malm University. Christina Bjerkn and Gunilla Nordin Fredrikson were reviewers of grants for the Swedish Research Council during 2011. Gunilla Nordin Fredrikson has also been a reviewer on the Regional Ethical Review Board in Lund since 2010. Ann Wennerberg was a reviewer of health research awards 2011for The Irish Health Research Board and of centres of excellence for the Research Council of Norway. Vitaly Kocherbitov was project application reviewer for the Danish Council for Independent Research. Anna Wennerberg was a
member of the Abstract Committee for the 20th Annual Scientific Congress of the European Academy for Osseointegration. Per Sthle is a member of the International Union of Theoretical and Applied Mechanics. Gunnel Svenster has been a member of the Scientific Advisory Board, Swedish Council on Technology Assessment in Health Care (SBU). Gunilla Nordin Fredrikson was consulted as a judgement expert for biomedical scientist programmes in Sweden by the National Agency for Higher Education and was also convener of the Evaluation Committee of DPLU (Diabetes Programme Lund University). She was also a reviewer for PhD student and mobility grant applications within the programme.
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CONTACT INFORMATION
Biofilms Research Center for Biointerfaces Faculty of Health and Society Malm University SE-205 06 Malm, Sweden Center Director: Dr. Anna Holmberg Tel: +46-(0)40-665 79 35 (Anna Holmberg) +46-(0)40-665 75 52 (Administrative Co-ordinator Eva Nilsson) E-mail: anna.holmberg@mah.se www.mah.se/biofilms Visiting address: Skne University Hospital, SUS (Entrance 49) Malm

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