Uncertainty of 10 ml pipette volume

Uncertainty of 10 ml pipette volume

The following components of uncertainty are taken into account: 1. Tolerance of the volume as specified by the manufacturer 2. Uncertainty due to the repeatability of pipetting 3. Uncertainty of the volume due to temperature difference from 20 C The calculation assumes that the pipette has been decently cleaned, that is no droplets are formed on the inner surface of the pipette after draining the liquid.

Model Equation:
{ Volume of the pipette The voulme is expressed as sum of three components. The V tol carries the value together with calibration uncertainty. The two other components carry only uncertainties, their values are set to zero. } V = Vtol + Vrepeat + Vtemp; { Uncertainty of the pipette volume due to temperature difference from 20 C } Vtemp = Vtol * * t;

List of Quantities:
Quantity V Vtol Vrepeat Vtemp t V: Vtol: Unit ml ml ml ml 1/C C Definition Volume of the 10 ml pipette Value of the volume together with tolerance uncertainty Repeatability uncertainty component of the volume Temperature component of the uncertainty of the volume (Uncertainty of the volume due to temperature difference from 20 C) Thermal expansion coefficient of water Difference of the liquid temperature from 20 C

Result Type B rectangular distribution Value: 10.000 ml Halfwidth of Limits: 0.03 ml Type A summarized Mean: 0 ml Standard Uncertainty: 0.04 ml Degrees of Freedom: 50

Vrepeat:

Date: 01/25/2004

File: 10_ml_pipette

Page 1 of 2
Generated with GUM Workbench TrainMiC Version 1.3.3.132

Uncertainty of 10 ml pipette volume

We estimate here that the repeatability standard uncertainty of pipetting is 0.4% of the pipette volume. We have found in our lab that this is a safe estimate. The users are recommended to substitute this estimate by real data obtained in their labs. Vtemp: : t: Interim Result Constant Value: 0.00021 1/C Type B rectangular distribution Value: 0 C Halfwidth of Limits: 4 C

In our lab it is reasonable to assume that the temperature does not differ from 20 C by more than 4 degrees. The users are advised to substitute this estimate by one that is obtained in their lab.

Uncertainty Budget:
Quantity Vtol Vrepeat t V Value 10.0000 ml 0.0 ml 0.00021 1/C 0.0 C 10.0000 ml 2.31 C 0.0439 ml rectangular 0.0021 0.0048 ml 1.2 % Standard Distributio Uncertainty n 0.0173 ml 0.0400 ml rectangular normal Sensitivity Coefficient 1.0 1.0 Uncertainty Contribution 0.017 ml 0.040 ml Index 15.6 % 83.2 %

Result:

Quantity: V Value: 10.000 ml Expanded Uncertainty: 0.088 ml Coverage Factor: 2.00 Coverage: manual

Date: 01/25/2004

File: 10_ml_pipette

Page 2 of 2
Generated with GUM Workbench TrainMiC Version 1.3.3.132