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Caerwyn Ash PhD1, Llinos Harris PhD2, Thierry Maffeis PhD3, Marc Clement PhD1, Gareth Stockman PhD1, Mike Kiernan PhD1, Godfrey Town4
1. CyDen Institute of Light Therapy 2. Medical Microbiology & Infectious Diseases, Swansea University 3. School of Nanotechnology, Swansea University 4. University of Wales, Faculty of Applied Design & Engineering, Swansea Metropolitan, Swansea, SA1 6ED, UK
Statement of Disclosure
The following potential conflict of interest relationships are germane to my presentation: Salary, equipment, travel expenses paid by CyDen Ltd, Swansea, UK
Background
Collaboration:
Background
Background
Investigative Study
In-Vitro Dose response
Various Fluence Various Wavelengths
Absorption Coefficients
Absorption Coefficients
Inter-Cellular Porphyrin
In-Vitro Methodology
Stock syrup kept at -80, agar plated and incubated for 72 hours at 37C Grade 0.5 McFarland solution using 2ml of Butterfields buffer 10,000 fold dilution with butterfield solution illuminate 300l in a 15mm well plate (random sequence) Plate 50l onto blood agar petri dish using a WASP Incubate for 72hrs at 37C under dark anaerobic conditions Count number of Colony forming units (CFU) Various wavelengths and fluence values
In-Vitro Methodology
Counting plates was time consuming Custom software to count total number of Colony Forming units (CFU) Also provided average, minimum and maximum of colony sizes. Information key for studying effect of metabolic changes
In-Vitro Results
In-Vitro Results
IPL covers Q-bands 414nm diode LED (soret) IPL (soret & Q-band) IPL (soret & Q-band also UV)
Illumination Control 414nm LED 330 1100nm IPL 400 1100nm IPL
Centrifuge at 4000rpm for 5min Aspirate solution Wash with Butterfield buffer
Asymmetrical separation
Asymmetrical separation
Asymmetrical separation
Unable to supply images! Images show asymmetrical separation of treated cells and cellular leakage
SEM Results - Summary This study has observed structural damages to membranes in illuminated bacteria's
Morphological changes Asymmetrical separation of treated cells (budding phase) Elongation of cells and cellular leakage
Discussion
This collaborative effort proved productive and beneficial to all groups Although effective in-vitro penetration depth in-vivo is an issue for short wavelengths The Soret band key in effective results in-vitro Consecutive treatments yield better results (data not presented) Illumination did not induce heat into bacteria or media.
Conclusions
This study bears relevance on current clinical research and better understanding on Acne pathology. Illumination of porphyrin with photons in the Soret region play a major role in P.acne cell necrosis. Benefits of using light therapy for acne treatment is well known wrt current antibiotics. Light of 407-420nm is not phototoxic to human cells For a one fold decrease in viable bacteria 73J/cm2 for 530-1100nm IPL 18.2J/cm2 for 414nm diode LED 10J/cm2 for 400-1100nm IPL
2011 Conference, Dallas Texas
Thank you
E: caerwynash@yahoo.co.uk