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Mobile Nest
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Rapid Recovery
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IVF Division
Concept
Spermfuge is an improvised centrifuge, dedicated totally for elevating the total
motile sperm recovery from the parent semen sample, by controlling/maintaining
the main parameter of "Temperature" thus aimed at Enhancing the ART results. The
instrument has been designed to regulate and subsequently maintain the "Critical"
inner chamber temperature before, after and during centrifugation. Maintenance of
this critical temperature throughout the entire sperm processing procedure helps the
sperm maintain their peak motility and enhanced linearity. The two of the most vital
parameters which act as a yard stick to gauge the effectiveness of an ideal sperm
wash. The existing dependency on RPM will be eliminated by on screen g-force
selection.
Design
The Spermfuge has been designed after taking into consideration detailed cellular
physiological demands, locomotion kinetics and pooling together ideal suggestions,
opinions, ideas and propositions of numerous Andrologists and Embryologists and
other professionals in the field of Human Reproduction. The Spermfuge with its
Temperature controlled chamber (room temperature to 42°C), programmable
microprocessor based controls, multiple tube selection, accurate g-force selection,
independent centrifugation and/or heating selection and other added advantages
make it an ideal state of the art instrument for use in an ART laboratory.
Multiple Programs
The Spermfuge facilitates user selectable 9 programs for different applications and
also for different set of protocols as standardized by various clinics, laboratories and
regulatory bodies. The user can select RCF, RPM, Tube Size, Temperature, Time for
each program by a digital encoder.
G - Force
The exact indication of G - Force helps the ideal sperm pellet formation by
subjecting the sperm to an exact and precise amount of G - Force unlike other
Centrifuges where G - Force is subject to RPM and the tube size. Now a days
centrifugation speeds are never reported in RPM as those numbers are not useful
for any one wanting to reproduce the method. (Being dependent Rotor radius.)
Operating Security
The Vision port in the lead at the center helps to confirm
rotational speed with a stroboscopic device. The safety
interlock in the lead prevents accidental opening once the
Rotor is operational. The maximum RCF is 800g which
prevents accidental damage to the sperm.
Maintenance
The Spermfuge is virtually maintenance free since it utilizes brushless drive motor.
The motor is extremely smooth even during running at the maximum speed. The
motor is electrically controlled during the acceleration and breaking with
smoothness.
Model : FL 17
Double Wall
Autoclave
Laboratory Autoclaves
Semi Automatic
Portable Autoclave
Semi Automatic
Gas cum Electric
Autoclave
Horizontal Sterilizer
Fully Automatic
Autoclaves GMP
Compliant Series
Acculeak Detector
Features:
Table top front loading autoclave
Fully Automatic micro-processor based control
Sterilization chamber made up of thick stainless steel sheet, tested for high
pressure
Digital timer for both wet and dry cycle
Auto drain of water to reservoir tank & also condensation of steam
Alarm after the total command is completed
Cycle time 10-15 minutes
Thus, EART provides a platform for perfecting Assisted Reproductive skills & Latest
technologies.
EART conducts various hands on courses in India with the help of renowned Indian
and International faculties. EART has successfully ensured that each participant gets
an adequate hands-on experience and project mock cycles, coupled with the
requisite theory lectures. One of the basic criteria of learning is getting an actual
hands-on experience. The hands-on session details are mentioned in the data
sheets of each course.
One-on-one training offers the maximum opportunity to learn at EART. Courses are
available for all assisted conception centre staff including medical, scientific
personnel. The unique one-on-one training makes the courses flexible in the course
content and the timing to meet your specific needs.
Apart from the above, EART also offers other services such as:
Turn key projects for IUI, IVF and ICSI Labs.
Calibration Services.
Quality Management System Implementation.
Accreditations
Sperm Fuge
Lab + Guard
Warming Blocks
Turnkey Projects
Rapid Recovery
System
IVF Division
Concept
Human oocytes are extremely sensitive to transient cooling 'IN VITRO' and modest
reductions in Temperature can cause irreversible disruption of the meiotic spindle,
with possible chromosome dispersal. Embryos subjected to such heat shocks have
high proportion of chromosomal abnormalities and this may contribute to the high
rates of pre-clinical and spontaneous abortion. Therefore, temperature control is of
paramount importance and should not be compromised in any way right from Ovum
Pick Up (OPU) till Embryo Transfer (ET).
Design
This is a handheld device with Li-ion rechargable battery. It is equipped with
heating element, Digital sensor, microcontroller, non-volatile memory and LED
indicators. When placed on base station, it gets conected electrically by its
contacts. These contacts help base station and the mobile device communicates
with each other and also charge the battery. The mobile nest when in mobile mode
continuously monitors the temperature with a 14 bit accuracy, corrects it several
times a second.
This yields a rock steady temperature profile even in varying surroundings. The
mobile nest continues to log for the power consumption index and temperature
abnormalities. These are downloaded to the base station when the nest is docked.
With monitoring and reporting features the user can be assured about the mobile
nest working accurately at every step. A transparent window facilitates easy viewing
of the follicular fluid.
Unique Portability
This Tubenest has been designed keeping in mind its portable nature. The
rechargeable battery is capable of running for 30 minutes at a stretch before it
indicates low battery level. The unique locking mechanism for tube allows
Embryologist to pour the aspirated follicular fluid into petridish for oocyte scanning
very easily. Easy grip design allows convenient handling and battery time facilitates
free movement in the LAB and OT.
Oprating Security
To ensure maximum oocyte survival without quality compromise, this unit has
various safety features. The Nest Tube has RED LED INDICATOR which flashes when
battery is weak. When the Nest is replaced in the Base Station, it checks the log
and indicates any overshoots. Moreover the Nest has a safety over temperature cut
out.
Sperm Fuge
Mobile Nest
Warming Blocks
Turnkey Projects
Rapid Recovery
System
IVF Division
Concept
The LAB + GUARD is developed to improve the laboratory air quality making it
nearly equal to clean air. Thus replenishing and subsequently nourishing the entire
laboratory, making it safe for the sensitive procedures to be executed. This is
achieved by a number of inclusions and their applications all of which are simple,
cost effective and user friendly. The positive pressure system uses the basic
principle of "Over Pressure or positive pressure in a room which restricts the
entry of outside air ". This is achieved by absorbing the circulating air outside the
IVF Lab (Room) and then subsequently passing the same through a series of 4
stage filters thus making it quite IVF friendly. Thus ultimately the effective,
circulating air in the IVF lab reaches a near clean air status, provides a positive
pressure, reduces the CFU count, eliminates the SPM, VOCs and CACs.
Design
The LAB + GUARD has been designed with State of the Art technology using 4
stage filtration system. The unique filter combination filters out air and gas
contaminant thus by giving most suitable air for gametes and embryos. LAB +
GUARD system contains a unique 4 stage filtration system containing Prefilter, Hepa
Filter and unique blend of activated carbon and alumina, which ensures wide
variety of Gases, particular contaminants are oxidized or are absorbed. The LAB +
GUARD has been designed to reduce the microbial air flora to the minimum level.
LAB + GUARD not only protects embryos and gammas but also protects
embryologists who is the most affected otherwise.
Operating Security
Every care has been taken in designing to make sure that air absorbed is
thoroughly cleaned for contaminants and to reduce bacterial count in IVF Lab. The
LAB + GUARD design also facilitates introduction of fresh air into the air circuit
which gives maximum safety from Carbon dioxide released into the IVF Lab by CO 2
Incubators and also it protects embryologists from gas mixtures released on work
stations.
Maintenance
The LAB + GUARD has automatic indication for Filter change and the design
facilitate extremely easy replacement of filters even by non technical staff. Other
than filter change it is virtually maintenance free
Return Blower
The return blower is utilised to circulate back the air of IVF lab to room where LAB
+ GUARD has been fitted.
Sperm Fuge
Mobile Nest
Lab + Guard
Turnkey Projects Are you giving thermal shocks to gametes and embryos?
Rapid Recovery Sudden and drastic temperature losses, recovery times and change in pH are
System minimized when using Fornax Warming Blocks. The unique aluminum alloy conducts
IVF Division heat and provides almost direct thermal contact with the heating surface. Dishes at
room temp when kept for incubation without the Warming Block take a prolonged
time to attain the set temperature compared to those dishes kept in preincubated
Warming Block, also ensures a safe and easy handling of dishes and test tubes in
IVF laboratory.
GB-4WDRA
Made from Aluminum Alloy with hard anodised surface.
GB-PDRA
Made from Aluminum Alloy with hard anodised of surface.
GB-717CA
Made from Aluminum Alloy with hard anodised surface.
GB-IDHA
Made from Aluminum Alloy with hard anodised surface.
Weight 44 gm.
Sperm Fuge
Mobile Nest
Lab + Guard
Warming Blocks
Rapid Recovery
System
IVF Division
Shivani Scientific Industries (P) Ltd has vast experience of more than 20 years of
designing and building pharmaceutical Laboratories as per WHO-GMP norms with
stringent requirement of sterility. The same experience has helped us to fulfill the
demanding needs of to-days IVF Labs.
A new Lab means start contacting designers, architects, sellers, resellers, vendors,
suppliers, agents, distributors to initiate slow, long and laborious process of
discussing dates and negotiating rates. This, you will agree, will further eat into
your most precious commodity "Time".
Alternatively you have the easier and simpler option: Contact US.
Embryology Services
We provide assistance in providing complete embryology services from Ovum Pick-
up to embryo transfer. Qualified and experienced team of clinicians and
embryologists are selected to provide these services.
Sperm Fuge
Mobile Nest
Lab + Guard
Warming Blocks
Turnkey Projects
IVF Division
In this case the heat energy in the incubator all the heated shelf can be directly
transmitted to the 4 well dish or Petridish if they are in contact.
Thermal Conductivity
Thermal Conductivity of air is 0.034 whereas Thermal Conductivity of Aluminium is
204 and Thermal Conductivity of Stainless Steel is 19.
Temperature changes during embryo culture when the dishes are taken out from
37º C incubator into lab where the temperature is anywhere between 24º C to 28º
C and also when culture media which are used in embryo culture lose the
temperature when they are droplet from flat surface.
Temperature changes recovery time and PA changes are drastically reduced when
Rapid Recovery System is used with regular CO2 Incubator. The new Rapid
Recovery System provides direct contact, which promotes heat further through
conduction to bottom of the culture dish.
Sperm Fuge
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Dr Franken, K Avari
Department of Obstetrics & Gynaecology,
University of Stellenbosch, Tygerberg Hospital,
Tygerberg, 7505, South Africa
February 2007
Sperm retrieval techniques form an integral Semen samples and sperm preparation
part of the assisted reproductive programme. Semen samples were obtained from
The success of sperm separation is measured normozoospermic donors and andrology
by the number of motile sperm retrieved from a referrals. Directly after collection samples were
given semen sample. The influence of various divided into 2 aliquots. One aliquot were
technical procedures and diluent media upon placed in a CO2 incubator at 340C while the
human spermatozoa has been tested in vitro second aliquot were left at room temperature.
(Jeulin et al., 1982). The percentage and A period of 30 minutes was allowed to stabilize
velocity of motile spermatozoa are often the semen temperatures before experimental
measured objectively using laser Doppler procedure. Prior to the experimental onset the
velocimetry or Hamilton Thorne IVOS 10 motion characteristics were recorded with the
computerized sperm analyzer (CASA). When CASA instrument in order to obtain initial
spermatozoa are incubated at 37 degrees C, values for the measured motion
rapid declines in both percentage motile and in characteristics.
velocity were observed with incubation periods
lasting more than 4-h (Jeulin et al., 1982).
Swim up separation
Human sperm incubation at room temperature
does not allow capacitation, although it does Motile sperm fractions were retrieved from the
not affect hFF-induced acrosome reaction in semen samples by mixing 0.5mL semen with
capacitated cells. The blocking effect is 1mL of Quinn’s Sperm Preparation medium.
overcome when spermatozoa are exposed to Two tubes were prepared for each experiment.
37°C (Maryn-Briggiler et al., 2002). Keppler et The tubes were then placed in 2 different
al., (2002) found significantly higher percent centrifuges, namely (i) SpermFuge SF 800
motility, mean average path velocity, straight (Shivani Industries, Mumbai, India), a highly
line velocity, lateral head displacement, and precise centrifuge with a temperature
percent hyperactivation in sperm at the 40ºC controlled chamber at 340C and (ii) Sigma
temperature (Keppler et al., 1999) bench top with no temperature control
facilities. Both centrifuges were set at
1500rpm (428xG) for 5 minutes. Following
the second washing procedure, both sperm
Objectives pellets were layered with culture medium and
left at a 450 angle at 340C for 60 minutes. After
the incubation period 0.5mL supernatant were
To evaluate the effect of temperature on the
removed from each tube and immediately
sperm motion characteristics and subsequent
analyzed for sperm motion characteristics.
outcome of sperm preparation.
1
Temperature Controlled Centrifugation
Results
Table 1 Results of semen parameters recorded in ejaculates and after preparation
with SpermFuge temperature controlled vs. room temperature centrifugation
Post Swim up
Ejaculates
340C Spermfuge Room temperature
% Hyperactivated sperm
BASELINE SWIMUP
2
Temperature Controlled Centrifugation
Baseline
Baseline
Baseline
Baseline
34 C
20 C
34 C
20 C
34 C
20 C
34 C
20 C
34 C
20 C
o
o
Mean 4.2 4.5 4.8 3.2 3.5 3.6 4.0 3.6 3.3 4.0 3.6 3.9 0.4a 1.2b 0.3c
SD 0.7 0.7 1.3 0.5 0.7 0.6 0.5 0.6 1.0 0.7 0.4 0.7 0.8 1.2 0.8
Transformed ARCSIN values showed a vs.b, a vs c and b vs. c Fisher’s exact test p=<0.05
Significant difference obtained in percentage hyperactivated sperm
References
1. Mortimer, D. (1990) Objective analysis of sperm motility and kinematics. In Keel B.A. and Webster,
B.W. (eds), Handbook of the Laboratory Diagnosis and Treatment of Infertility. CRC Press, Boca Raton,
pp. 97-133.
2. Holt WV and Palomo MJ (1995) Optimization of a continuous real-time computerized semen analysis
system for ram sperm motility assessment, and evaluation of four methods of semen preparation,
Reproduction, Fertility and Development 8(2) 219 - 230
3. Jeulin C, Serres C, Jouannet P. (1982) The effects of centrifugation, various synthetic media and
temperature on the motility and vitality Reprod Nutr Dev,22(1A):81-91
4. Makler A., Deutch M., Vilensky A., Palti Y. (1981) Factors affecting sperm motility. VIII. Velocity and
survival of human spermatozoa as related to temperatures above zero International Journal of
Andrology, 4 (5), 559-569.
5. Maryn-Briggiler CI, Tezon JG, Miranda PV, Vazquez-Levin MH. (2002) Effect of incubating human
sperm at room temperature on capacitation-related events. Fertil Steril 77, (2) 252-256.
6. Keppler EL, Chan PJ, Patton WC, King A. (1999) Aggregation of human sperm at higher temperature
is due to hyperactivation. Arch Androl 42; 35-39.
DFR22022007-F3
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