Paper (Practical)

MICR 502 3(2-2) GENERAL AND SYSTEMIC VIROLOGY Q 1: - Why we use chromium Chloride in IHA? (2) Q 2:- Define 4HA; calculate 8HA of a sample if the titer of sample is 1: 1024. (4) Ans (128) Q 3:- Define secondary cell line (2) Q 4:- Draw a figure representing different routes of inoculation. (4) Q 5:- You are provided a solution of 100mM of tannic acid, calculate the conc. of tannic acid Required for PHA in 20ml final volume. (2) (.01 or .001) Q 6:- Draw figures for Indirect, direct, Sandwich Indirect and sandwich Direct ELISA and also label them. (4) Q 7:- Why we use monoclonal Abs in sandwich ELISA. Define Conjugate? (2) Q 8: - Enlist name of four techniques to concentrate virus. (2) Q 9: - Enlist names of four cell lines (Please write complete names not abbreviations)(2) Q 10:- Deign an experiment in order to know protective Ab titer against a virus. (6) Paper (Practical) MICR 502 3(2-2) GENERAL AND SYSTEMIC VIROLOGY Q 1: - Why we use chromium Chloride in IHA? (2) Q 2:- Define 4HA; calculate 8HA of a sample if the titer of sample is 1: 1024. (4) Q 3:- Define secondary cell line (2) Q 4:- Draw a figure representing different routes of inoculation. (4) Q 5:- You are provided a solution of 100mM of tannic acid, calculate the conc. of tannic acid Required for PHA in 20ml final volume. (2) Q 6:- Draw figures for Indirect, direct, Sandwich Indirect and sandwich Direct ELISA and also label them. (4) Q 7:- Why we use monoclonal Abs in sandwich ELISA. Define Conjugate? (2) Q 8: - Enlist name of four techniques to concentrate virus. (2) Q 9: - Enlist names of four cell lines (Please write complete names not abbreviations)(2) Q 10:- Deign an experiment in order to know protective Ab titer against a virus. (6) Paper (Practical) MICR 502 3(2-2)

GENERAL AND SYSTEMIC VIROLOGY Q 1: - Why we use chromium Chloride in IHA? (2) Q 2:- Define 4HA; calculate 8HA of a sample if the titer of sample is 1: 1024. (4) Q 3:- Define secondary cell line (2) Q 4:- Draw a figure representing different routes of inoculation. (4) Q 5:- You are provided a solution of 100mM of tannic acid, calculate the conc. of tannic acid Required for PHA in 20ml final volume. (2) Q 6:- Draw figures for Indirect, direct, Sandwich Indirect and sandwich Direct ELISA and also label them. (4) Q 7:- Why we use monoclonal Abs in sandwich ELISA. Define Conjugate? (2) Q 8: - Enlist name of four techniques to concentrate virus. (2) Q 9: - Enlist names of four cell lines (Please write complete names not abbreviations)(2) Q 10:- Deign an experiment in order to know protective Ab titer against a virus. (6)