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World Leaders in the Development & Supply of Enzymes & Reagents for Fructan Research & Analysis
Megazyme is pleased to announce that it can now offer ultra-pure, recombinant, affinity purified endo-inulinase (E-ENDOIAN) and exoinulinase (E-EXOIAN). These enzymes have been produced at its research facility in Bray, Ireland. These enzymes are available separately for research applications, and have also been incorporated into the Fructan and Fructan HK analytical kits offered by Megazyme.
Fructan Kit (K-FRUC) suitable for the measurement of even trace levels of fructan (inulin or fructooligosaccharides, FOS) in plant and food products e.g. in grain and in infant formulations. The removal of interfering sugars through borohydride reduction also reduces the terminal reducing fructose in partially hydrolysed fructans. This leads to an underestimation of up to 20% for highly depolymerised fructans. However, if the pure FOS ingredient is available, the level of underestimation can be determined and allowed for. Fructan HK (K-FRUCHK) gives no underestimation of fructan, even with highly depolymerised samples. However, the method is not recommended for samples containing high levels of D-glucose, D-fructose, sucrose and/or maltose.
Megazyme International Ireland, Bray Business Park, Bray, Co. Wicklow, Ireland t + 353 1 286 1220 e info@megazyme.com w www.megazyme.com

Enzymes & Kits for Fructan Analysis & Research

Analytical Kits
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Fructan (K-FRUC) - AOAC Method 999.03; AACC Method 32-32.01 - Incorporating highly purified enzymes including recombinant exo-inulinase and endo-inulinase Fructan HK (K-FRUCHK) - Incorporating highly purified enzymes including recombinant exo-inulinase and endo-inulinase - For quantitative measurement of fructan and FOS

Pure Research Enzymes

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exo-Inulinase (E-EXOIAN) (recombinant, affinity purified) NEW endo-Inulinase (E-ENDOIAN) (recombinant, affinity purified) NEW

Purified Enzymes for Specific Analytical Applications

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exo-Inulinase plus endo-Inulinase mixture (E-FRMXLQ) - For measurement of fructan according to AOAC Method 997.08 exo-Inulinase plus endo-Inulinase mixture (E-FRMXPD) - For measurement of fructan according to AOAC Method 997.08 Amyloglucosidase (E-AMGFR100 & E-AMGFR500) - For use in fructan determination according to AOAC Method 997.08

Soluble Chromogenic Substrates

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Azo-Fructan (S-AZFR5) - For the measurement of endo-inulinase

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