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CELLSTAR
Tissue Culture Products
Multiwell Plates Cell Culture Flasks Cell Culture Dishes

CELLSTAR Multiwell Plates

6, 12, 24, 48, 96 Well Clear Polystyrene Plates for Cell Culture Applications

Greiner Bio-One offers a complete product line of excellent quality CELLSTAR Multiwell Plates in 6, 12, 24, 48 and 96 well configurations for adherent and suspension cell cultures. Flat well bottoms are standard on the 6, 12, 24 and 48 well plates, while the 96 well density offers flat, round and conical well bottoms. Further, the 96 well half-area plate reduces surface area and volumes to eliminate the need to change to a higher well format. Low evaporation lids and chimney-well designs significantly reduce the risk of edge effect and well-to-well cross contamination. Stackable with or without lids, an advanced design facilitates continuous heat and gas exchange within single or stacked plates. Uniform plate dimensions comply with ANSI/SBS 1 recommendations for robust performance with standard and automated equipment.
See page 4 for ordering information.

PRODUCT FEATURES
Plate well formats: 6, 12, 24, 48, 96 and 96 half-area. Growth area (cm2): 9.6, 3.9, 1.9 , 1.0, 0.3 and 0.15 respectively. Work Volume: 2- 5 mL, 2 - 4 mL, 0.5 -1.5 mL, 0.5 -1 mL, 25-340 l and 15-175 l respectively. Uniform dimensions to ANSI/SBS 1 recommendations. Ergonomic, space-saving, easy peel-apart packaging. Lot # and Best Before Date on each pack.

ADVANCED APPLICATIONS
Cell Proliferation and Viability Assays: Tetrazolium dyes MTT & XTT (stains mitochondria) Sulforhodamine B (SRBbinds proteins) Trypan Blue (diazo dye stains dead/apoptotic cells) Neutral Red (cell nucleus) Cytotoxicity Assays / Apoptosis (LDH release on cell death) Colorimetric EIA (Enzyme ImmunoAssays): Primarily 96; 24 & 48; uses cell supernatants, cell extracts, serum, plasma, urine. Light microscopy analyses of parallel cultures.

Alphanumeric well coding. Chimney well designs. Single position (non-reversible) Condensation Ring Lid enables optimal gas exchange with the lowest possible evaporation for minimal edge effect. Adherent (TC Treated) and Suspension Cell Culture Plates. Available with CELLCOATTM protein coated surfaces (Poly-D-Lysine, Poly-L-Lysine, and Collagen) for enhanced cell adherence. Custom surfaces on request. Sterile, Non-Pyrogenic (< 0.06 EU/mL), Non-Cytotoxic and RNase / DNase / human DNA free. Base 6, 12, 24 well CELLSTAR Multiwell Plates in tandem with Greiner Bio-One ThinCertTM cell culture inserts for Co-cultivations, Chemotaxis (i.e., Cell Migration and Invasion; Protocols Available), Transport Studies, Primary Cultures and TEER assays.
Application Guide available to determine best membrane for specific use

CELLSTAR Cell Culture Flasks

T25, T75 and T182 Cell Culture Flasks

In addition to multiwell plates, Greiner Bio-One offers standard and filter cap cell culture flasks with growth areas of 25cm2, 75cm2 and 182cm2 made of high-grade optically clear polystyrene. For adherent cell growth, the surfaces are treated to optimize cell adhesion and proliferation. Non-treated flasks for suspension culture are also available in 50, 250, 550 and 650 mL formats. Our flask design provides optimal access for cell scrapers and pipettes, secure stacking and engraved and printed graduations for easy filling.
See page 4 for ordering information.

PRODUCT FEATURES
Growth area: 25cm2, 75cm2 and 182cm2 Flask volumes: 50, 250, 550 and 650 mL Adherent (TC Treated) and Suspension Cell Culture Flasks White printed graduations on one side, and engraved graduations on the other side White writing field Standard plug-seal and 0.2 m pore filter caps Each flask individually integrity tested Canted neck for pipette or scraper access Raised rims for secure stacking Excellent optical clarity Lot # and Best Before Date on each flask Sterile, Non-Pyrogenic (< 0.06 EU/mL), Non-Cytotoxic, USP class VI tested and RNase / DNase / human DNA free

ADVANCED FILTER CAP MEMBRANE

Filter caps have a patented PET/PTFE membrane with a defined pore size of 0.2m, high mechanical strength and increased hydrophobicity to prevent wetting on the inside. Optimal gas exchange is guaranteed while the risk of contamination is minimized.

Comparison of different membrane types according to their pore size distribution

Top view (left) and cross-section (right) of capillary pore membrane: The capillaries are filled with copper and the PET is removed by etching. (electron micrograph)

CELLSTAR Cell Culture Dishes

Sterile Polystyrene Tissue Culture Treated

CELLSTAR dishes from Greiner Bio-One are manufactured from high-grade virgin polystyrene allowing for unsurpassed distortion-free optical clarity, a feature especially useful during microscopic applications. Furthermore, these dishes are tissue culture treated for optimal cell attachment and proliferation. All Greiner Bio-One dishes have stacking rings for simple storage and ease of handling. Vented dishes allow for optimal gas exchange and reduced condensation. Dishes are sterile, in user-friendly packaging and are available in 35mm, 60mm, 94mm, 100mm and 145mm diameters.
See page 4 for ordering information.

CELLCOAT Products
Protein Coated Cell Culture Vessels

In addition to the CELLSTAR line, Greiner Bio-One offers protein coated tissue culture vessels under the CELLCOAT line which is comprised of cell culture vessels coated with proteins of the extracellular matrix (ECM) or synthetic proteins (Poly-D- and Poly-L-Lysine). Besides an improved adhesion and proliferation of primary cells and various cell lines, CELLCOAT plates are highly suitable for serum-free and serum-reduced cell cultivation and experiments which include additional washing steps or stressful procedures, (e.g. transfection). Moreover, the differentiation of individual cell types can be enhanced through the protein-coating.
Please contact your Greiner Bio-One sales representative for details.

APPLICATIONS:
Improved adhesion Improved cell proliferation Cell adhesion assays Receptor-ligand binding studies Reduced-serum or serum-free cultivation Improved growth of primary cells Differentiation of individual cell types

ADVANTAGES:
Increase in isolation and cultivation efficiency Ready-to-use products: immediate use, time-saving consistent quality Collagen Type I and Poly-Lysine coated products storable at room temperature

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Ordering Information
Greiner Cat. No. 657160 657165 657185 665180 665165 665102 662160 662165 662102 677180 677165 677102 675180 655180 655182 655160 655162 655185 650180 650160 650185 651180 651160 CELLSTAR Multiwell Plates Pack Qty. Case Qty. 6 well plate, tissue culture treated, with lid, sterile 1 100 6 well plate, tissue culture treated, with lid, sterile 5 120 6 well plate, suspension culture quality, with lid, sterile 1 100 12 well plate, tissue culture treated, with lid, sterile 1 100 12 well plate, tissue culture treated, with lid, sterile 5 120 12 well plate, suspension culture quality, with lid, sterile 1 100 24 well plate, tissue culture treated, with lid, sterile 1 100 24 well plate, tissue culture treated, with lid, sterile 5 120 24 well plate, suspension culture quality, with lid, sterile 1 100 48 well plate, tissue culture treated, with lid, sterile 1 100 48 well plate, tissue culture treated, with lid, sterile 5 120 48 well plate, suspension culture quality, with lid, sterile 1 100 96 well half area plate, tissue culture treated, with lid, sterile 8 32 96 well plate, tissue culture treated, F-bottom, chimney well, w/condensation ring lid, sterile 1 100 96 well plate, tissue culture treated, F-bottom, chimney well, w/condensation ring lid, sterile 10 160 96 well plate, tissue culture treated, F-bottom, chimney well, no lid, sterile 1 100 96 well plate, tissue culture treated, F-bottom, chimney well, no lid, sterile 5 100 96 well plate, suspension quality, F-bottom, chimney well, with lid*, sterile 1 60 96 well plate, tissue culture treated, U-bottom, with lid, sterile 1 100 96 well plate, tissue culture treated, U-bottom, no lid, sterile 1 100 96 well plate, suspension quality, U-bottom, with lid, sterile 1 60 96 well plate, tissue culture treated, V-bottom, with lid, sterile 1 100 96 well plate, tissue culture treated, V-bottom, no lid, sterile 1 100

Greiner Cat. No. 690160 690170 658170 660160 661160 690175 658175 660175 661175 690190 658190 660190 661190 690195 658195 661195

CELLSTAR Cell Culture Flasks Pack Qty. Case Qty. T25 Flask, TC treated, sterile, plug seal cap, 50mL, canted neck 10 200 T25 Flask, TC treated, sterile, plug seal cap, 50mL, canted neck, measuring grid 10 200 T75 Flask, TC treated, sterile, plug seal cap, 250mL, canted neck 5 120 T182 Flask, TC treated, sterile, plug seal cap, 550mL, canted neck 5 50 T182 Flask, TC treated, sterile, plug seal cap, 650mL, canted neck 4 40 T25 Flask, TC treated, sterile, filter cap, 50mL, canted neck, 10 200 T75 Flask, TC treated, sterile, filter cap, 250mL, canted neck 5 120 T182 Flask, TC treated, sterile, filter cap, 550mL, canted neck 5 50 T182 Flask, TC treated, sterile, filter cap, 650mL, canted neck 4 40 T25 Flask, suspension culture, sterile, plug seal cap, 50mL, canted neck, 10 200 T75 Flask, suspension culture, sterile, plug seal cap, 250mL, canted neck 5 120 T182 Flask, suspension culture, sterile, plug seal cap, 550mL, canted neck 5 50 T182 Flask, suspension culture, sterile, plug seal cap, 650mL, canted neck 4 40 T25 Flask, suspension culture, sterile, filter cap, 50mL, canted neck, 10 200 T75 Flask, suspension culture, , sterile, filter cap, 250mL, canted neck 5 120 T182 Flask, suspension culture, sterile, filter cap, 650mL, canted neck 4 40

Greiner Cat. No. 627160 627170 628160 633160 632171 633171 664160 639160

CELLSTAR Cell Culture Dishes Pack Qty. Case Qty. 8.7cm 2 growth area, vented, TC-treated, sterile 10 740 35 x 10mm, 35 x 10mm, 0.97cm2 per ring growth area, vented, TC-treated, sterile, 4 inner rings 10 500 60 x 15mm, 21cm2 growth area, vented, TC-treated, sterile 10 600 94 x 16mm, 58cm2 growth area, vented, TC-treated, sterile, hybridoma dish 10 480 94 x 16mm, 58cm2 growth area, non-vented, TC-treated, sterile 10 480 94 x 16mm, 58cm2 growth area, vented, TC-treated, sterile 10 480 100 x 20mm, 58cm2 growth area, vented, TC-treated, sterile 15 360 145 x 20mm, 145cm2 growth area, vented, TC-treated, sterile 5 120

Quality & Surface Treatment Information

CELLSTAR Tissue Culture Products are manufactured from high quality, optically clear resins and are tissue culture treated to provide a surface with excellent adherent properties for uniform cell growth and proliferation. Stringent manufacturing conditions and quality control measures ensure no contamination and guarantee only the hightest class of products reach our customers.

Greiner Bio-One Products are:

Free of DNase, RNase and human DNA DNases and RNases are essential and ubiquitous enzymes which protect the living organism against the invasion of foreign DNA and foreign RNA. DNases and RNases are cell components which also occur in body fluids. Because of the great potential for transmission (e.g. skin, hair, etc.), overalls, gloves and hairnets are worn in all production areas. The most sensitive method available, the PCR method, is used to check for contamination with DNase, RNase or human DNA. DNase, RNase and human DNA free products are labeled in our catalog. Free of endotoxins Endotoxins are complex lipopolysaccharides which are components of the outer membrane of gram-negative bacteria. These substances are the most widespread and effective members of a group of so-called pyrogens that cause fever in humans. The LAL assay (Limulus Amoebocyte Lysate) used to test for freedom from endotoxins complies with the FDA guidelines for medical products (12/87). Greiner Bio-One works with the sensitive detection limit of < 0.06 EU/mL. Endotoxin-free products are labeled in our catalog. (The US Pharmacopoeia guidelines stipulate that eluates from medical devices [e.g. washing liquids from consumable materials] must show endotoxin levels of < 0.5 EU/mL. For materials that come into contact with cerebrospinal fluid, a level of < 0.06 EU/mL is required.) Sterile Sterilization by irradiation is required for the application of many of our products. The validation of the necessary radiation dose is conducted in accordance with EN 552, ISO 11137 and ANSI/AAMI ST31/ST32. On the basis of this standard, the so-called bioburden (germ level) is established for each product and the necessary radiation dose is determined. This determination is repeated at regular intervals. The sterility of the sterilized products is monitored. We guarantee a sterilization dose of SAL10-3 and a shelf life of 5 years for sterilized products. Testing of cell culture products All cell culture products are physically surface-treated and are tested with different cell lines. Morphology, cell growth and cell vitality are monitored in the process. A broad spectrum of cells, ranging from primary cells, mammalian cells and human cells to insects cells, enable comprehensive testing. We guarantee 4 years stability on the surface treatment of all cell culture products. Free of cytotoxicity In many experiments toxic effects on cells or tissue cultures play an important role particularly in the field of in vitro diagnostics. The relevant culture vessels and all objects that come into direct contact with the cells and tissues must therefore be free of cytotoxic substances. All cell culture relevant end products are checked for freedom of cytotoxicity. The detection of cytotoxicity is evaluated with mammalian cells according to EN ISO 10993.

Surface Treatment Information

CELLSTAR and CELLMASTERTM " Products for adherent cell culture are tissue culture treated using a special physical process. The treatment leads to the inclusion of polar groups, such as carboxy and hydroxyl groups, making it hydrophilic. CELLCOAT " In addition to tissue culture treated vessels, Collagen Type 1, Poly-D-Lysine and Poly-L-Lysine protein coated vessels are available for cells that requre a protein surface for culture.

Quality Management
Quality Standard in Compliance with DIN EN ISO 9001 and DIN EN ISO 13485 Greiner Bio-One has been certified according to DIN EN ISO 9001 since 1994, and has been re-certified in 1997, 2000 and 2003 according to the new DIN EN ISO 9001:2000 through the continuing high standard of our production processes, quality controls and organization (certificate " p. A I 2). Since 2004 we have also been certified according to DIN EN ISO 13485:2003 to fulfill the high international requirements for manufacturers of medical devices (certificate " p. A I 2). A total quality approach encompassing the latest production technologies, strict control of conditions and materials, as well as the qualification and development of personnel ensure constant improvement in both our products and processes. Our customers trust in us for over 30 years certifies our high quality and our dedication to further quality improvement. CE Marking, Compliance Since December 2003 it has been mandatory for products which fall within the scope of the European Directives 93/42/EWG for medical devices and 98/79/EG for In Vitro Diagnostics to be correctly labeled by bearing a CE mark. In line with the definitions in these directives some of our products have been considered as medical or In Vitro Diagnostic devices and as such have been labeled in accordance with the regulations. In our catalog these products are labeled with a CE mark.

Quality Assurance & Quality Control

Quality is the most important criterion in the manufacturing of our products. For this purpose, we have a control system which monitors the physical, chemical and biological quality of our products. The Quality Inspections start with the incoming raw material is continued in production processes and ends with the dispatch of the finished product. Strict controls, conducted according to the legal provisions and specific standards, accompany the product. Ongoing quality training ensures that our employees are informed about the applications of our products and the quality requirements placed on them. All products can be traced back to their production period, machine operators and tools, all the way back to the starting materials used. With each delivery of high quality goods, our customers receive a quality certificate and a guarantee of performance which commits us to maintaining our high quality standards.

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