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The Sysmex XT-series and XS-series haematology analyzers incorporate the ingenious Fluorescence Flow Cytometry technology, which was successfully introduced with top-of-the-end SysmexXE-2100 range. These X-series analyzers are equipped with excellent capabilities offering optimal differentiation between normal cells and pathological cells.
Q. How Polymethyne dye is useful in differentiation of cells? A. Polymethyne dye allows the use of laser diodes which are significantly economical and more reliable than Argon ion lasers. The effect of staining of Polymethyne dye on mature and Immature cells is depicted in following illustrations. Mature cells before staining Immature cells before staining
Due to higher DNA/RNA content, immature cells scatter more Fluorescence Light than mature cells. Q. How scattergram is generated from Fluorescence Light? A. The scattergram shown explains how scattergram is generated from Fluorescence (SFL) and Side Scattered Light (SSL) signals. Cells rich with RNA (high SFL) but with simple structure (low SSL) will be differentiated in population marked as 1). Cells poor with RNA (low SFL) but with simple structure (low SSL) will be differentiated in population marked as 2). Cells rich with RNA (high SFL) but with complex structure (high SSL) will be differentiated in population marked as 3). Cells poor with RNA (low SFL) but with complex structure (high SSL) will be differentiated in population marked as 4). Q. How does a scattergram of normal sample and abnormal sample look like? A. As shown in the illustrations of scattergrams, DIFF channel uses Fluorescent Activated Differentiation (FAD); Side Fluorescence Light (Y-axis) and Internal Cell structure i.e. Side Scatter Side Scatter Light (X axis).
Normal Sample
DIFF
MONO
Abnormal Sample
FAD enables X-series analyzer to differentiate not only mature WBCs, but also mature and immature WBCs. By staining the cells analytical sensitivity of cell counter is enhanced and signalto-noise ratio is increased. Reportable ranges are extended and interferences are reduced.
DIFF
Atypical Lymph Promyelo
Myelo
Blast
Metamyelo
Band
NRBC
PLT Clumps