The article is about how multiple antibiotics become resistant to different infections, therefore becoming a major threat to hospitals

and communities. This maybe caused by over prescribing medication. Researchers have tested E.coli, on different antibiotics, and concluded 12 of the antibiotics becomes resistance to the E.coli. They also tested disinfections the cetrimide monocationic surfactant, which is used in hospitals for cleaning floors, burns, wounds, and to wash hands. They were testing if the AR E.coli are resistant to the cetrimide and also if it reduces the nosocomial pathogens in the hospitals. The researchers collected different samples of pubs, wound swabs, blood and, urine from Private and Government hospitals to do the test. The microorganisms that were used were AR E.coli and E.coli. The researchers tested different microorganisms in their testing ,for example when they were stating the antibiotics resistance, the sample become positive with E.coli. was furthered tested using Rapid Hi Coliform agar. MacConkeys agar and Endo agar was used to isolates the E.coli. it was also maintained proper nutrients and at 4c. In order for them to get accurate testing the researchers did multiple testing. By doing the test they found 12 different antibiotics resistance to E.coli. COULD NOT UNDERSTAND The researchers tested the Cetrimide through the determination of the broth micro dilution method. They also used the Time Kill Study which they exposed the time of AR E.coli to Centrimide. The AR E.coli was inculcated in two colonies from overnight culture on MacConkeys agar, which was incubated for 37c. Following the incubation of the cells, they were harvested and washed with sterile cold distilled water. At that point the Cetrimide was dissolved in 10 ml, to get the final concentration and bacteria were added. Time and the concentration of Cetrimide are very important because if it used appropriately the Cetrimide can damage the cell changes in E.coli. Which would result in the nocomial pathogens reduction in hospitals. I found the article to be of particular interest to me, because I recalled a time in Africa when I was told by a health worker to finish the antibiotic but there is no explanation of why. I strongly believe people should be given this vital information, on how people can become resistant to antibiotics. Such knowledge should be commonplace due to its importance in sustaining the health of the human body. The article was a bit to difficult to understand due to the scientific jargon that was used. In conclusion, I found the article peaked my interest mainly because the researchers also gave a table of antibiotics they tested resistant to E.coli. I found the article very important because it discussed the implications of antibiotic resistance. As a future nurse, it is crucial to learn how to disinfectant according to the direcis, hence reducing the spread of pathogens in the hospital. 1 summarize the article 2 what microoganism are detailed in the article? What information a u given about microorganisms 3 is tha article intreasting/ what did u learn? What was lacking? Why is the article important? Why should u care about the information?