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Results
8.0E+06 Viable Cell Density
100
Direct Adaptation and Continuous Growth of expresSF+®
Percent Cell Viability
Cells in EX-CELLTM 420 7.0E+06
expresSF+® shaker cultures adapted easily from Sf-900 II SFM 6.0E+06 80
9.00E+06
Freeze/Thaw Evaluation of expresSF+ ® Cells in
8.00E+06 EX-CELLTM 420
7.00E+06 Table 1 indicates the density and viability of cells frozen in
6.00E+06
fresh EX-CELLTM 420 with 10% DMSO at 24 hours post-thaw.
These results indicate that expresSF+® cells can be frozen and
Viable Cells/mL
5.00E+06
easily recovered in EX-CELLTM 420.
4.00E+06
Table 1. Density and Viability of expresSF+® Cells
3.00E+06
24 Hours Post-Thaw
2.00E+06 Cell Density Cell Viability
1.00E+06 (cells/mL) (%)
Flask 1 2.32 x 106 92.1
0.00E+00
0 2 4 6 8 10 12 14 16 18 20 22 24
Flask 2 2.76 x 106 86.3
Day
Flask 3 3.12 x 106 91.8
Figure 1: expresSF+® cells growing in Sf-900 II SFM (open circles)
were directly subcultured into EX-CELLTM 420 (shaded circles) at
the sixth passage (Day 12).
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Conclusions
• expresSF+ ® cells can be transferred directly into
EX-CELLTM 420 from Sf-900 II SFM without an adaptation
period.
• expresSF+® cells grown in EX-CELLTM 420 achieve densities
of 6-7 x 106 cells/mL with viabilities greater than 95%.
• expresSF+ ® cells can be frozen and recovered in
EX-CELLTM 420 with only the addition of 10% DMSO.
References
1. Protein Sciences Corporation, Meriden, CT
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or any other legal theory. SAFC Biosciences expressly disclaims any warranty against claims by any third party by way of
infringement or the like. THIS PRODUCT IS INTENDED FOR PURPOSES DESCRIBED ONLY AND IS NOT INTENDED FOR
ANY HUMAN OR THERAPEUTIC USE.
Additional Terms and Conditions are contained in the product Catalog, a copy of which is available upon request.
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