Name: _________________________________ Date: _____________ Period: ________

Active and Passive Transport: The Play

Starring: Ms. Taylors Advanced Biology Class In Shakespeares famous work, As You like It, he says All the world is a stage and all the men and women merely players. In the following week, we will be acting in our own play to demonstrate the different types of transportation inside and outside a cell. Every person will be receiving a role in the play, and each role is present in a couple of the 4 acts in our play. As you are watching and participating in our play, make sure you are following along with the reflection questions that come at the end of each act. All reflection questions will be due typed up or hand-written on Monday December 3rd.

Make sure you have answered all 12 of them!!!!

Glue your part in the space below:

Day 1: Act 1
Lead Roles: Water molecules, phospholipids Supporting Actors: glucose, carbon dioxide, Ca ions Back-stage: Before class, read pages 74-76 in your textbook and then define the following vocabulary words in the vocabulary section of your binders: Passive transport Concentration gradient Equilibrium Diffusion Osmosis Reflection questions: 1. Why are the phospholipids always moving around each other?

2. Why do the water molecules always move to the side that has the fewest water molecules? Make sure to include concentration gradient and diffusion in your answer. 3. What is it called when water molecules diffuse through a selectively permeable membrane? 4. Why could water and carbon dioxide molecules get through the lipid bilayer, but glucose couldnt?

Day 2: Act 2
Lead Roles: Phospholipids, calcium ions, calcium ion channel Supporting Actors: glucose, water, glucose channel Back-Stage: Read pgs. 78-80 and then define the following words in the vocabulary section of your binder: Ion channel Facilitated diffusion Reflection Questions: 1. Can a glucose molecule get through the calcium ion channel? Why or why not? 2. Did calcium generally move with its concentration gradient or against it?

Day 3: Act 3
Lead Roles: Phospholipids, Glucose, Glucose carrier protein, ATP Supporting Actors: calcium ions, glucose channel Back-stage: Read page 81 and define the following words in the vocabulary section of your binder: Active transport Carrier protein Reflection Questions: 1. Which way did the glucose carrier protein transport the glucose molecules? With or against their concentration gradient? 2. Why could only glucose molecules pass through the channels? 3. What was necessary for the glucose carrier protein to be able to transport the glucose across the membrane?

Day 4: Act 4
Lead Roles: Water, Phospholipids, Calcium Ions, Calcium Ion Channel, Glucose, Glucose carrier protein, ATP Reflection Questions: 1. Which act(s) of our play would fall under the title passive transport? Why? Give evidence from this activity to explain your answer. 2. What are the scientific names of the different kinds of passive transport demonstrated? 3. Which act(s) of our play would fall under the title active transport? Why? Give evidence from this activity to explain your answer.

Roles: Water You and your peers are all water molecules. You are small enough that you can easily slip through the phospholipid bilayer, however be careful to always be aware of the position of the other water molecules! Your job is to always be where there are least of them! Phospholipid You and your peers are all individual phospholipids in the membrane. This means that you are arranged a certain way (paired up across from each other, as it is a bilayer). However, the phospholipid bilayer is not solidremember, it is fluid. This means that each lipid is always moving in relation to the other lipids, whether that means switching places next to each other or across from each other, vibrating in place, etc. Just make sure that the lipid bilayer NEVER STANDS STILL!!! Also, remember the properties of a phospholipidthe outside is hydrophilic (waterloving), and the inside is hydrophobic). This means that certain molecules can easily get through the bilayer, while as other molecules have more trouble. Small molecules, such as water and carbon dioxide, easily slip through either way, as they are small with negligible charge, however, large molecules, or anything with a charge CANNOT get through. So, if any glucose molecules or calcium ions try to get through the bilayer, BLOCK THEM! Calcium Ion You and your peers are calcium ions. You are very small, however, you have a positive charge, which means that you cannot travel directly through the lipid bilayer. You must travel through a calcium ion channel. Note: you must always be aware of the location of your fellow calcium ions. Try to always be on the side of the cell with the least of them. Calcium Ion Channel You and your partner are both components of a calcium ion channel. This means that you can only let calcium pass through, and at that, you can only let one through at a time. However, it does not matter which way calcium is travellingyou always let then through whether they are on their way out of the cell or into the cell. To show that you are one protein, lock hands in bridge-formation, as we do in the game London Bridge is Falling Down, and insert yourself somewhere in the lipid bilayer. Note: you should NEVER let any other molecule try to get through because your shape only matches that of a calcium ion. So put your bridge down, and block them if they try to get through! Glucose You and your peers are all glucose molecules. Glucose molecules are fairly big (for a molecule), so they cannot pass through a membrane individually, but need the help of a carrier protein, specifically, a glucose carrier protein. Note: try to always be on the side that has the fewest glucose molecules, as you want as much space around you as possible. Glucose Carrier Protein

You and your partner are both members of the glucose transport protein. All cells need glucose all the time, and you are one of the key players in providing that glucose. You take glucose from outside the cell and transport it in. However, this can only be done using energy from an ATP molecule because you are transporting glucose molecules against their concentration gradient. So, this is how you set up: stand next to your partner in the lipid bilayer. One person must face the inside of the cell, and the other person must face the outside of the cell. Every time you transport a glucose molecule, the person facing the inside of a cell must gently grab an ATP molecule by the arm, and the person facing the outside of the cell must grab a glucose molecule gently by the arm. Then, when both of you have your parts, slide away from each other, pull the glucose molecule into the cell, and release the ATP molecule. Note: You only transport glucose molecules. If any others try to get through, BLOCK THEM! ATP You and your peers are all ATP molecules that float around on the inside of the cell. Every time the glucose carrier protein needs to transport glucose across the membrane, they need one of you for energy.