Sample Abstract and Guidelines for Preparation of Abstracts Ideal Typeface Size

Epstein-Barr virus particles in squamous cell carcinoma of the bronchus. PJ BALOYI, JM NKOSI*, S MATHIBI. Department of Pathology, University of Azania, Republic of Azania baloyi@UAZ.ac.aa This study was undertaken to determine the presence of Epstein-Barr (EBV) DNA in squamous cell carcinoma of the bronchus. Formalin fixed, paraffin embedded blocks of bronchial squamous cell carcinoma diagnosed between 2000 and 2006 were retrieved from the files of the Department of Pathology at the University of Azania. Sixty cases were selected that showed normal appearing overlying epithelium, the submucosa of which were infiltrated by a squamous cell carcinoma. All patients gave a history of tobacco use for longer than 10 years. A control group consisted of 40 wax blocks of biopsies taken of non-neoplastic epithelial lesions of the bronchus in long term tobacco users. A standard PCR reaction was employed to amplify the EBV BAM HI W- fragment using a nested primer set (Ross et al 1992). The PCR sensitivity was adjusted to detect one EBV genome per reaction. All results were confirmed with Southern blotting using the EBV BAM HI W-fragment as a probe. EBV DNA was demonstrated in 14/60 (23%) in the carcinoma group and 2/40 (5%) in the control group. This difference was statistically highly significant (p<0.01) Stratification of the experimental group according to age, showed a significantly higher rate of positivity (40%) in patients younger than 40 years (p<0.05) The results demonstrated that EBV DNA was present in significantly more cases in the squamous cell carcinoma group than in nonneoplastic epithelium. EBV may be an etiological factor in bronchus carcinomas in the population studied, particularly in the age group below 40 years. This study was supported by Grant X20, MRC, Azania.

Abstract preparation instructions: In order to achieve a uniform style for all abstracts published in the Book of Abstracts and become eligible for publication in the South African Journal of Science, authors are requested to conform to the following: Font size: 10 pitch Title Block Font Style: Times New Roman Title: Limit your title to twelve or fewer words and type in bold. Authors: Starting after the title, list the initials (first and second initials) followed by the surname in capital letters. Place an asterisk (*) after the name of the presenting author. Asterisk only one name per abstract. Institution: The name of the institution(s) should follow the last authors name. Contact Address: If available, include the e-mail address of the corresponding author at the end of title block. Content of the Abstract All text of the abstract should be fitted within the box provided on the abstract form. Abstracts that have been reduced in size before submission or printed in small type (smaller than Font 10) will be rejected. The content of the abstract must contain the following subsections: Objective of investigation: The first sentence should be a brief statement on the objectives of the investigation. This should be followed by: Methods used: A brief description of the experimental method used. Results: Essential results including core data and where appropriate, statistics should be clearly stated in the abstract content. Conclusion: The conclusion(s) of the study should be underlined. Supporting agency or grants: Where supporting grants have been received, the agency and grant number should be quoted.

Publication of Abstracts in the South African Journal of Sciences: After the Congress, abstracts will be submitted to a panel of referees who will select those that are eligible for publication in the prestigious South African Journal of Sciences. This will be done at no extra cost and will provide those that meet the following requirements, the additional benefit of a published scientific abstract in a reputable journal of science. Requirements: 1. All research accepted for publication in the South African Journal of Science should be new, not published before and not in print. 2. The abstract should conform to the outline given for publication in the book of abstracts. 3. The final decision for publication lies with the editor of the South African Journal of Science.

FINAL ABSTRACT FORM

Follow the attached example and outline. Initials & Surname of Presenter: Initials & Surname of CoWorker(s):

Postal Address of Presenter:

Postal Code

Telephone Number of Presenter: Facsimile Number of Presenter: E-mail Address of Presenter:

Code: Code:

No: No:

Your abstract will only be accepted for publication in the Book of Abstracts if: 1. Your submission conforms to the guidelines as shown on the attached example 2. The presenter, as indicated by the asterisk in the title block, has registered for the Congress COMPLETE THE ABSTRACT FORM ELECTRONICALLY AND SUBMIT ON-LINE BY CLICKING ON THE SUBMIT BUTTON. If you are unable to submit the abstract on-line, save this page and e-mail it as an attachment to kmossan@medunsa.ac.za or deidre.raubenheimer@mrc.ac.za SUBMIT