Labspec Software

LabSpec Software
USER GUIDE
Part Number: 31 087 064
COPYRIGHT
Copyright 1999 Dilor-Jobin Yvon-Spex, HORIBA group division. All rights reserved. No parts of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system, or translated into any language or computer language, in any form or by any means, electronic, mechanical, magnetic, optical, chemical, manual or otherwise, without prior written permission of Dilor-Jobin-Yvon - Spex, HORIBA group division (FRANCE).
NOTICE TO THE USER

This manual should not be construed as any representation or warranty with respect to the unit named herein. Occasionally, changes or variations exist in the unit that are not reflected in the manual. Generally, should such changes or variations exist and affect the product significantly, a release note would accompany the manual. In such a case, be sure to read the release note before using the product. Trademarks: LabSpec is a registered trademark of Instruments S.A.; IBM, PC-XT, PC-AT, Windows 95 are registered trademarks of International Business Machines Corporation; MS-DOS is a registered trademark of Microsoft Corporation.
Version 1.1 Dilor-Jobin Yvon-Spex All Rights Reserved Printed in France MM-NP/Labdoc.FM/0399 August 1999
Jobin Yvon-Spex HORIBA Group 16-18, rue du Canal 91165 LONGJUMEAU CEDEX (France) Tel: (33) 01 64 54 13 00 - Telex: JOBYVON 602882 F Fax: (33) 01 69 09 93 19 - (33) 01 69 09 07 21 Internet site: http://www.JobinYvon.com , http:// www.Isainc.com 244 ter, rue des Bois Blancs 59000 LILLE (France) Tel: (33) 03 20 08 12 20 - Fax: (33) 03 20 08 12 28/29 Email: 100302.2741@Compuserve.com Wiesenstrasse 4 - D-64625 BENSHEIM (Germany) Tel: (49) 062 51 40 42 - Fax: (49) 062 51 648 71 Email: 100271.330@Compuserve.com 3880 Park Avenue, EDISON - New Jersey 08820 (USA) Tel: (1) 908 549 71 44 - Fax: (1) 908 549 51 25 2-4 Wigton Gardens - STANMORE Middlesex HA7 1BG (Great Britain) Tel: (44) 181 204 81 42 - Fax: (44) 181 204 61 42 Via Cesare Pavese 35/AB - 20090 OPERA (Milano) (Italy) Tel: (39) 2 57 60 30 50 - Fax (39) 2 57 60 08 76 P.O. Box 56 -2400 AB ALPHEN a/d Rijn (Netherlands) Tel: (31) 0/1720 33323 - Fax: (31) 0/1720 39532
Dilor S.A.
Dilor GmbH
ISA Inc.
ISA UK Ltd
ISA ITALIA Srl
ISA Nederland
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Dilor-Jobin Yvon-Spex, Horiba group division
With its long experience in research and manufacture of scientific instruments (since 1819), the DilorJobin Yvon-Spex equipment, division of HORIBA Group, uses state of art technology for optical, mechanical, electronic and computer management. The result is unequaled comfort and flexibility of use, with less human error, for the kind of precision and reproducibility of measurements that are required in laboratory work today. Last but not least, Dilor-Jobin Yvon-Spex instruments are designed and manufactured with total user safety in mind.
Our Internet Site:
http://www.JobinYvon.com http://www.Isainc.com
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Warranty
Dilor-Jobin Yvon-Spex, HORIBA Group division, guarantees each instrument it manufactures for a period of one (1) year, including parts and labor. Our obligations during this period will be limited to the repair or replacement -at our discretion- of every instrument returned to the factory, shipment prepaid, during a period of one (1) year from the date of delivery to the original purchaser, providing that Dilor-Jobin Yvon-Spex or its representative gives prior approval.
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About the LabSpec User Guide
Your approach to the LabSpec User Guide depends on what you want to do and how much you already know. The following list gives a brief description of each section of this manual. 1. 2. 4. 5. Introduction Reference section Tips section Index
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Contents
Icons list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Menu Bar Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
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1.1 Icons list

Icon 1 Icon 2 Icon 3 Icon 4 Icon 5 Icon 6 Icon 7 Icon 8 Icon 9 Icon 10 Icon 11 Icon 12 Icon 13 Icon 14 Icon 15 Icon 16 Icon 17 Icon 18 Icon 19 Icon 20 Icon 21 Icon 22 Icon 23 Icon 24 Icon 25 Icon 26 Icon 27 Icon 28 Icon 29 Icon 30 Icon 31 Icon 32 : Standard cursor of work - - - - - - - - - - - - - - - - - - - - - - - - - page 13 : Peak elimination - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13 : Shape correction - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13 : Zoom - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13 : Horizontal shift - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13 : Vertical shift - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13 : Intensity adjustment - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13 : Shift - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13 : Manual addition - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13 : Manual multiplication - - - - - - - - - - - - - - - - - - - - - - - - - - - page 14 : Manual labeling of peaks- - - - - - - - - - - - - - - - - - - - - - - - - page 14 : Move peak maxima - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 14 : Fit peak width - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 14 : Delete active object - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 14 : Load a file- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 14 : Save the active object - - - - - - - - - - - - - - - - - - - - - - - - - - - page 14 : Cursor normalization - - - - - - - - - - - - - - - - - - - - - - - - - - - page 16 : Normalization - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 16 : Print page - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 16 : Information about object - - - - - - - - - - - - - - - - - - - - - - - - - page 16 : Multiwindow automatic spectra recording - - - - - - - - - - - - - page 17 : Mathematical treatments - - - - - - - - - - - - - - - - - - - - - - - - - page 17 : Baseline correction - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 19 : Correction- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 21 : Profile - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 23 : Filtering - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 26 : Fourier filtering - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 29 : Peak fitting - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 30 : Integral calculation - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 33 : Palette window - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 33 : Zoom - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 34 : Spectral mapping procedure- - - - - - - - - - - - - - - - - - - - - - - page 35
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Icon 33 Icon 34 Icon 35 Icon 36 Icon 37 Icon 38 Icon 39 Icon 40 Icon 41 Icon 42 Icon 43 Icon 44 Icon 45 Icon 46 Icon 47
: Model procedure - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 36 : Video image - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 36 : Spectrum adjustment - - - - - - - - - - - - - - - - - - - - - - - - - - - page 36 : Spectrum recording - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 37 : CCD image adjustment - - - - - - - - - - - - - - - - - - - - - - - - - - page 37 : Spectral images recording - - - - - - - - - - - - - - - - - - - - - - - - page 37 : Data size - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 38 : Acquisition parameters - - - - - - - - - - - - - - - - - - - - - - - - - - page 41 : Detector parameters - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 42 : Morphological imaging - - - - - - - - - - - - - - - - - - - - - - - - - - page 43 : Calibration of the XY table - - - - - - - - - - - - - - - - - - - - - - - page 43 : Configuration - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 45 : Scheme- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 46 : Stop of spectra recording - - - - - - - - - - - - - - - - - - - - - - - - - page 46 : Different types of cursors - - - - - - - - - - - - - - - - - - - - - - - - page 46
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1.2 Control panel
Laser: Value of the exciting line. Filter: There are 6 neutral filters with the optical densities 0.3, 0.6, 1, 2, 3 and 4. filter [---] = no attenuation (P0), [D0.3] = P0/2, [D0.6] = P0/4, [D1] = P0/10, [D2] = P0/100, [D3] = P0/1000 and [D4] = P0/10000 Hole:Value of the hole aperture. : go to minimum value of the hole : go to maximum value of the hole : Initialize hole, go to minimum value of the hole and go back to the previous position.
Spectr: Position of the spectrograph : go to zero order : go to the diode position : Initialize spectrograph, go to zero order and go back to the previous position. Time: Recording time (seconds).and number of accumulations Options: : 1800g/mm and 300, 600 or 1200 g/mm. Be careful to initialize spectrograph if the grating is changed. : Three different objectives can be chosen: 10, 50, 100. : Enter a name for the next recorded spectrum. The program will add an incremental number after the entered name. Be careful to give no more than 8 characters.
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Icon 1 Standard cursor for working
1.3 Description of the panel icons

Icon 1 Standard cursor for working
Allows to come back to the standard cursor after having selected one of the other tools that are presented by the icons 2 to 11. This tool allows to move the different cursor found in the acquisition window. Icon 2 Peak elimination
This feature removes peaks that we do not want to observe. Icon 3 Shape correction
This feature permits to modify a spectrum by moving each point of the spectrum. Icon 4 Zoom
Press the left button of the mouse and draw a rectangle which select the part of the spectrum to zoom. Icon 5 Horizontal shift
Move the abscissa scale of the spectrum Icon 6 Vertical shift
Move the ordinate scale of the spectrum Icon 7 Intensity adjustment
This feature allows to adjust the scale in intensity of a spectrum. Icon 8 Shift
Move the scale of a spectrum
Icon 9
Manual addition
This option allows the user to add a constant to the active spectrum. This is a manual method, using the mouse: 1 Press the mouse left button +Const , 2 Move the spectrum up and down, then press OK to validate.
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Icon 10
Manual multiplication
This option allows the user to multiply the active spectrum by a constant. This is a manual method, using the mouse. The procedure is the same as the one for manual addition. Icon 11 Label of the peak
The wavelength or wavenumber of a peak appears if the left button of the mouse is pressed. The label will be deleted by pressing the right button of the mouse. Icon 12 Move peak maxima
By pressing the left button of the mouse, the label of a peak can be moved precisely. The label selected is in white, by default it is the last one, but another color can be selected in the window Bands Parameters (icon 45 then button Bands) Icon 13 Fit peak width
By pressing the left button of the mouse, the width of a band can be changed. As for the icon number 5, the bandwidth in the window Bands parameters can be selected (icon 45 then button bands) Icon 14 Delete
Delete the active object (e.g. acquired spectrum) in the active window. Icon 15 Load
Load from the disk. The appropriate previously saved file will automatically be opened. Icon 16 Save the active object (spectrum)
The active object like an acquired spectrum can be saved on the hard disk. SPECTRA. When a spectrum is acquired, it is not saved but remains in the Random Access Memory (RAM) of the computer. If several spectra are acquired, different color dots will be displayed on the right edge of the window. Each dot takes the same color as the displayed spectrum and each dot works as a radio toggle button. Each time an acquisition is performed a new color button is added.The full names of the spectra are indicated in the object menu command.
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The active object like an acquired spectrum can be saved on the hard
To save one of the displayed spectra, activate the spectrum window, select the desired spectrum by pressing the radio button, then choose one of the following methods 1Click on the following icon: then enter the file name, its location and format.
2From the file Main Menu, select the Save As option, then enter the file name, its location and format. Before saving, it would be useful to complete the information list about the spectrum (operator, laser power, etc...) by pressing the icon . Some parameters (hole, slit, spectro, grating, time, accum, date) are automatically updated. LabSpec file formats. The following is a list of the various file formats that LabSpec supports to save the acquired spectra or images: - Compressed Tiff (*.tsf): - Extended Tiff (*.tsf): - Dilor format (*. ms0): - Text format (*.txt): It is a compressed format, specific to the Labspec software, It is a specific format of the Labspec software This format can be used with the other DILOR softwares. This is an ASCII mode format which uses two columns: wavelength/Wavenumber and intensity, without header.
- Spectra Calc format (*.spc), This format is used by SpectraCalc and Grams processing softwares. IMAGES. The save procedure always saves the content of the active window. To save an image, activate the window that contains all the spectra. If the single spectrum window choise is activated, only this spectrum will be saved, and not the whole spectral image. To save a video image, activate the window that contains the video image.
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Image file formats Extended Tiff (*.tvf) It is a binary format for spectral images, or time kinetic. If a spectral image is saved in this format, the models can be saved together with the image. Two images can be added in one file. Compressed Tiff (*.tvf) It is a compressed format Text format (*.txt) The data given by this format are the intensities; the wavelength or wavenumber parameter is losen. Standard Tiff (*.tif) This option should only be used for 2D array of data, not for spectral images. Color Tiff (*.tif) This format is used to save color Video images. Icon 17 Cursor normalization
Bring cursor to the center of the active window. Sometimes the cursor is not visible, in this case move the mouse. Icon 18 Normalization
Automatic scaling optimization of a spectrum or an image. Icon 19 Print page
Video images, spectral images or spectra can be inserted and printed on a prepared layout page. There is another way to print the data: transfer them to another Windows 95/Windows 98 software using the copy pictures or copy data choices from the EDIT menu. This Print icon prints out the objects of the active window The layout page can be configured with the option print set up from the EDIT menu. Icon 20 Information about object
Information about an acquired spectrum or image. Most of the information is inserted automatically into this table, enter the name of the sample, the name of the operator, the laser power, the name of the file and some eventual remarks. Once performed, press OK button to validate.
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Icon 22 Mathematical processing
Icon 21
Multi-Window automatic spectra gluing
When several regions have been acquired, these regions can be glued with the Multi-Window Option feature. The result is displayed in a new window and the previous separate regions are not deleted.
Example: The figure above gives the example of a 3 windows acquisition, the first one covers 200-500 cm-1, the second one 1200-1800 cm-1 and the third 2300-2700 cm-1. If the whole spectrum between 200 and 3000 cm-1 must be acquired, select the first line and the numbers 200 and 3000 in the box Window limits . If the combine feature has not been validated before the acquisition, it is possible to perform it after an acquisition by pressing the button COMBINE ; once activated, it will combine all the spectra of the active window. Icon 22 Mathematical processing
These mathematical procedures can be applied to spectra, spectral profiles, spectral images and Video images. Available calculations: - Calculate a function of the active spectrum, - Add a constant to a spectrum, - Multiply a spectrum by a constant, - Add, subtract, multiply, divide two spectra,
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A: Constant for addition This option adds a constant to a spectrum. Enter the constant value in the + field, then press the button OK to validate. Constant for multiplication This option adds a constant to the spectrum. First, enter the value of the constant in the first field. Then, press the button OK to validate. B: Operations between two spectra These four buttons allows the user to add, subtract, multiply or divide two spectra. The first spectrum is the current active spectrum but the program will ask for the second spectrum, if more than two spectra are displayed in the window. C: Definition of the function In this field, enter the mathematical functions to activate. The available functions are: +, -, +, /, log(), exp(), sin(), cos(), tan(), atan(), abs(), sqrt(), step(), power xa and any combinations of these. A function of two variables x and y can be also calculated. x will refer to the active spectrum, y to a spectrum loaded in the RAM (Random Access Memory). The program will ask to define y when the button FUNC is pressed. If only one spectrum is loaded in memory x and y will be the same. To perform the calculation, press the button OK .
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Icon 22 Mathematical processing
Icon 23
Baseline correction
The spectra baseline can be computed and subtracted by pressing this button The main procedure is to build up point by point a baseline, to fit at best to the spectrum and then subtract. .
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An automatic procedure can also be used for the computation of the baseline, button AUTO . A:Operations for the baseline Four choices are available: 1- Ins/Del: It permits to select the points for the computation of baseline. Validate the point with the left button of the mouse or deletes it with the right button. 2- +Const: Add a constant to the baseline. Validate this button and move the baseline with the cursor. 3- *Const: Multiply the baseline by a constant. The procedure is the same as the one for +Const. 4- Zoom: It allows to magnify a spectrum zone.
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Icon 24 Correction
B: Deviation Choose the deviation (1% is normally appropriate): that is to say the residual difference between the calculated baseline and the optimal one. The automatic calculation of baseline depends on this coefficient. C: Attachment A best fitting can be done automatically with this command. It attaches the baseline to the spectrum. It could be useful for the correction of baseline of spectral images.
Icon 24
Correction
When this option is chosen, the following window appears and gives access to some utilities for the correction of spectra and spectral images.
GET: The active spectrum in the single spectrum window can be taken as a reference for the options normalization , sub , add , div , mul and corr . The reference spectrum is stored in a correction window . This spectrum is removed with the command del
THR
This option is used for the images. For every spectrum of an image, this function activates to zero all the intensity values that are lower than thr(%) of the intensity of the principal peak. This function allows to remove the lowest peaks Threshold(%) It is the limit which is expressed in percentage of the value for the threshold for the elimination of weak peaks.
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ZERO Translate the spectrum or all the spectra until the lower point is brought to zero. Normalization Normalization of the spectra to the part of corrector spectrum that is inside the green cursors in the correction window
MUL, SUB, ADD, DIV These options allows the user to multiply, subtract, add or divide the corrector spectrum to all the spectra of a spectral image.
Spectrum correction
Fits at best the corrector to the spectrum by means of a multiplicative factor k . The corrector multiplied by the factor k is then subtracted from the spectrum. The default fitting is applied to the whole spectrum. If a double cursor is activated, the fitting is done only inside the cursor.
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Icon 24 Correction
Icon 25
Profile
This window allows the user to glue spectra together in a 3D array, change or insert spectrum in a set of spectra. A profile of a 2D image (CCD image, video image, mapping...) can also be performed
Profile
To profile an image, select the cursor line on this image (if a horizontal line has been chosen, do not forget to check in the HOR box).Then press PROFILE . A rectangular cursor can be also selected to start profile generation.
Insert
Insert the active spectrum in a set of spectra. With the cursor, choose the position of the spectrum in the image.
Remove
Remove spectrum from a set of spectra.
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Change
Exchanging of spectrum in a set of spectra.
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Icon 24 Correction
Add
Add one spectrum at the end of the set of spectra. In the window Spectrum select the first spectrum and press ADD . Do the same procedure with the others spectra, but dont forget that the selected spectrum is always added at the end of the set of spectra.
Extract profile
Extract one spectrum from a set of spectra.
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Icon 26
Filtering
The Filtering feature can be applied to any data object like spectrum, image, spectrum profile, spectral images ... Each of these data is a multidimensional cube and each of these has its own specific dimension defines as follow: Spectrum: one dimension (wave number), Image: two dimensions (X and Y axis) Spectrum profile: two dimensions (time and wave number) Spectral image: three dimensions (X, Y and wave number) ... To perform a filtering at least one dimension must be selected. The figure below shows the filtering parameters: Enter here the weight of the elements of the mask.
Data sliding bar Load customized filtering Launch filtering
Here below is the example of a 3-dimension filtering screen:
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Icon 24 Correction
Size: Size of the mask (the number of points around the filtered central datum that are involved in calculations of the filtered value) Dimension: As explain above, dimension is the parameter on which is applied the filtering process. As an example, if you want to filter a spectrum, the dimension will be F (wave number). For an image, the dimensions available are F , X or Y . If a wave number filtering is asked for a spectral image, all the spectra will be filtered. Symmetry: You can choose in the box symmetry if the weight of the points of the mask are symmetrical in respect to the point to be filtered or not. Type: There are five types of filtering: - Ordered: Once the mask characteristics has been chosen, the software analyzes the data levels (intensities) located in the mask then replace the central datum level (intensity) by the level determines using one of the following options: - MAX: the maximum level value finded in the mask, - MIN: the minimum level value finded in the mask, - MED: the middle level value, - INC: the intermediate value between the maximum value and middle value, - DEC: the intermediate value between the minimum value and the middle value. Example: the values of the mask are: 50 10 5 4 8 9 32 68 75 41 3 The first step is to order the point: 3 4 5 8 9 10 32 41 50 68 75 The results will be: MAX = 75, MIN = 3, MED = 10, INC = 50, DEC = 5. - Average : Choosing this option will substitute the selected datum value (0 in the mask) by the average value calculates from all of the values of the mask. The calculation process uses the following formula: i=1 yc = n - PeakElm: The PeakElm option modifies the spectrum only if a sharp peak is found. A peak is detected if its intensity is higher than the enterd threshold value. The level of the threshold must be enterd in the PEAK field. A value of one means that the threshold is fixed at 100% of the average value calculated from mask values, 2 means 200% etc... If I is the intensity of the point to modify and Im the average intensity of the mask points, then the value of I is replaced by Im if I<Im+I*peak
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- Linear: The Linear option performs a linear combination of the values of the mask. The coefficients (wi) of the linear combination can be introduced directly in the mask. In this case the value of yc is:
yc =
wi yi i =1
i =1
Special types of filtering can be saved and loaded again with LOAD button. A major example of linear filtering is the derivative. In this case, the mask will contain the coefficients 1, -1, 0 or also 1, 0, -1 if derivative is performed on 3 pixels. - Polyn: Polynomial filtering is only available for one-dimensional filtering. The degree of the polynom interpolates the values in the mask. Enter the degree value in the ORDER field.
General: How to perform a filtering ?

To perform a filtering on an object, follow the steps: 1- Choose the correct dimension by pressing on the corresponding button. 2- Select the type of filtering and the mask size. 3- Press FILTR to perform the filtering.
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Icon 24 Correction
Icon 27
Fourier transform (filtering)
This option allows the calculation of the Inverse Fourier Transform of a spectrum. It can also be used for images. By applying mathematical functions to the interferogram, it is possible to correct some defaults of the spectrum Dimension: Choose the dimension where you want to calculate the IFT (inverse Fourier transform) (Freq. for a spectrum, X or Y for an image) Function: Mathematical filtering: exp (b*t), trafic, Low band Apodisation: Mathematical filtering (apodisation): 1-(t/T), 1-(t/T)2, cos (t), Remark: the parameters of the different correction equations can be easily modified by adjusting graphically their curves with the mouse DFT: After a modification of the interferogram this icon allows you to calculate the Fourier transform and modify the spectrum of the active window IFT: Calculation of the inverse Fourier transform Z%: Increase or decrease the abscissa axis of the interferogram DFT IFT : Automatic calculation of the Fourier transform : Continuous calculation of the inverse Fourier transform.
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Icon 28
Peak fitting
The Peak Fitting feature detects the peaks using different shaped bands, like gaussian/lorentzian or even custom ones.
There are two methods of detection of the peak: 1. AUTO: The automatic detection of peaks is obtained by pressing the button SEARCH .You must take care of the position of HEIGHT an NEIGHBOUR scrolling bars. HEIGHT/NEIGHBOUR: A peak is detected only if it is a local maximum and if its intensity is higher than a threshold. The threshold is fixed by HEIGHT scrolling bar. The width of the local region is defined by NEIGHBOR scrolling bar. If a spectrum extends from frequency f1 to frequency f2 with a maximum MAX , a minimum MIN and bar% is the position of the cursor bar, then the threshold is: THRESH = MIN + (MAX-MIN)*bar% and the width of the local region is: ZONE = (f1-f2)*bar%
2.
Icon n 11
CLEAR: Delete all the selected peaks. The label button allows you to put the label of the different peaks by using the icon .
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Icon 24 Correction
The sum button displays the sum of all bands, i.e. the fitting. The shapes button displays the shape of each single band. With the button attach , the label can be fixed to the related peak. BANDS: In the window BANDS we have the result of the peak fitting procedure. (This list of results can be saved with the icon SAVE) For the images, if you want to have the fitting of the position, the surface, the amplitude or the width at half maximum, in the Bands window , activate the buttons p , s , a or w .
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FUNCTION: There are four possibilities: - Gaussian/Lorentzian y = a*(g*exp(-((x-p)*(x-p))/(w*w))+(1-g)/(1+4*((x-p)*(x-p))/(w*w)) - Gaussian y = a*exp(-((x-p)*(x-p))/(w*w)) - Lorentzian y = a/(1+4*((x-p)*(x-p))/(w*w)) - Line y = c*y + a
VAR: Select the variables of the different functions. In this window, select the variables of the different functions. You can choose the maximum and the minimum of each variable. Or you can fix one of these parameters. NOTICE: if you fix one parameter in this window, for example p , this variable will be fixed for all the bands that you want to fit, so if you want to fix the parameter p for just one band, you have to do it in the window BANDS by checking in on the desired parameter) - The variable p is the position of the band. - The variable a is the amplitude of the band. - The variable w is the width at half maximum. - The variable s is the surface of the band. - The variable g is the Gaussian/Lorentzian ratio. APPROX: It calculates the theoretical shape of each selected peak. It permits to correctly initialize the peak fitting parameters TIME: Maximum time for calculation
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Icon 29 Integral calculation
Deviation factor The optimization of the fitting parameters is done iteratively with the method of maximum gradient. In each step (j) the deviation of the interpolation from the real spectrum is calculated summing over all spectral pixels from f1 to f2. d(j) = (If-Sf)*(If-Sf) Where If is the intensity at frequency f and Sf is the value obtained at frequency f by the peak fitting. Deviation is defined so that if deviation > |d(j)-d(j-1)|/d(j), the calculation is stopped as it is near enough to the solution. Icon 29 Integral calculation
To calculate an integral under a band, zoom the band of interest. Select the green cursor, position the first and the second cursor with the mouse. Then press the icon
Icon 30:
Palette window
It allows you to choose the colors for drawing of plane and 3D representations of images. - grey: - FALSE: Select a grey level palette. Select a 16 false colors palette
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- TRUE: Select a 3 (red, green, blue) colors palette, each color with intensity levels. This drawing style is only used when superimposing two images. - OTHERS Palettes are the same as grey but the black and the white are substituted by other fundamental colors. You can also adjust the contrast and the brightness of your image
Icon 31
Zoom
Zoom in either X, Y, wave number and intensity dimensions. For images and spectra. Extract If you are just interested by a part of a spectrum or if you want to limit the wave number domain of a spectral image, you can use this button: First, you have to select the part of the spectrum (or the spectral image into all spectra window ), for that you have to make a zoom by using the icon . Then press the button Extr : on the screen you will have the selected part .
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Icon 31 Zoom
Icon 32:
Spectral mapping procedure
This window describes the algorithms which are used for the mapping. It gives us some spectral information: - Description of the cursors (Red, Green, Blue) There are 3 types of cursors (green, blue and red), there are in all spectra window. You can set them around the interested bands and if the options red , green and blue are active, it gives you the mapping of the different cursors. - Green/blue It is the ratio between the contents of green and blue cursor of the window all spectra, either in mode Raman and Fluo. -Decomposition It is the procedure used to make some spectra models (this option is in mathematical treatments ) In this procedure, each spectrum of a spectral image is fitted with a sum of model spectra. The relative importance of each model in the fitting can be mapped. -Extract procedure This procedure is used to have the spectrum in a single spectrum window. Deconvolution It gives you the percentage for the contribution of each of the model components in the different points of the mapping. Summa It gives you the error made by the calculation of the% of the different model components. Raman/Fluo Using of baseline correction for cursors (red, green, blue) operation. Raman mode discards baseline contributions while Fluo mode calculates the integral from zero.
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Icon 33
Model
It is the decomposition of each original spectrum of an image as a sum of model spectra. 1. Choose the spectra that will become MODELS : they can be already saved on the disk, or they can be particular spectra that you can extract and save from your spectral image. If the spectrum is a particular one extracted from the image, save it. Do the same thing with the other interesting spectra. Load all the selected spectra and put them overlapped in a window (for that use the option behaviour of the Format menu command). Activate the first selected spectrum and press the button GET. Do the same for all the selected spectra: a window is then created with all the model spectra . In the single spectrum window, there are: - The models with relative intensity. - The experimental spectrum in particular point of the image. - The result of the fitting. You will see in the mapping window additional pictures that correspond to each one of the model . You can overlapped them to see the relative contribution of each of the spectra in particular point of the image (for that use the option behaviour of the Format menu command). 7. You can save a whole spectral image with the model compounds choosing the tvf format. When you will load this image, the decomposition will automatically appear. Video image
2. 3. 4.
5.
6.
Icon 34
Video image grabbing. The video image is frozen by pressing the icon STOP (n46). The type of cursor (buttons at the right lower edge of the window) indicates the type of object you choose to record in imaging mode. Icon 35 Spectrum adjustment
If you want to maximize the signal and adjust experimental conditions you can use this icon This option is a spectrum adjustment (continuous recording of spectra, the new spectrum refreshes the old one and so on), so it helps you in maximizing the signal and in adjusting experimental conditions.
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Icon 37 CCD image adjustment
Icon 36
Spectrum recording
This option permits to start spectrum recording without moving table in the point indicated by the point into the video image. It is the simplest procedure to record a spectrum. With this icon you can also do a multi window recording of spectra by choosing the parameters in the window Spectral windows parameters (icon 21) Icon 37 CCD image adjustment
This option permits to record a CCD image. The procedure is the same as the one for adjusting a spectrum (refer to the help of the icon ). But to record the CCD image you have to press the icon . This option is useful to check the position of the spectrum on the chip in order to select the good binning : You can see precisely the spectral domain of interest. Icon 38 Spectral images recording
If you have an XY motorized table, or if you want to do a time kinetic recording, you can select some points on the video image to have their spectra. This procedure is used for recording spectral image or line scanning recording, time kinetics and also spectra positioning the XY table in the video image, instead of the icon that records the spectra without moving table. To activate this icon you first have to record a video image, and you have to choose the type of cursor that will define the area to analyze. Meaning of these different cursors: - Point cursor : You select or delete a point with the right button of the mouse, you move a point with the left button of the mouse N spectra are recorded point by point along the line.
- Inclined line
- Horizontal line
N spectra are recorded with Laser scanning along the line or point by point. An array of N*M spectra is recorded inside the rectangle with laser scanning or point by point. : You can use these cursors if you are just interesting by a part of the sample. It is a point by point recording.
- Rectangle
- Elliptic and polygon
After the acquisition three windows will be opened
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Single spectrum window Three cursors are available and are selected with the buttons at the right lower corner: - A red single cursor for the wavelength and the intensity readout. - A double green cursor for the readout of the width of a band. - A yellow cursor composed of a continuous line and two dotted lines. The continuous line automatically positions to the maximum of the band in the neighborhood of the mouse arrow and the two dotted lines position at the half maximum of the band. All spectra window This is the full representation of a spectral image. All the spectra that compose the spectral image are displayed overlapped. In this window, three cursors allow the selection of the bands for the selective spectral mapping. Spectral mapping window This window contains the mapping of some spectral features. For example: mapping of a band, ratio between two bands, spectral models decomposition etc... Icon 39 Data size
This option permits to select the parameters of spectral images (Xsize, Ysize, Zsize, number of spectral points). If you want to do a time Kinetic recording, the choice of the time interval is done in this window.
X
In the box size, you can select the number of points (spectra) that you want to record along each line. It is the binning (cf. Spectral) in spatial dimension (the short one). Or if you want you can choose the step between two measurements in the box Step&Binning.
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Icon 39 Data size
Y
In this box you can select the number of lines of a spectral image.
Spectral.
This box contains the number of spectral points read out of the detector. The number of data points along the wave number direction can be less than the number of pixels because you can choose to readout some pixels together. This technique takes the name of binning. If two pixels are binned together, their intensities are summed up, thus you gain in S/N, but, of course, you loose spectral resolution. The binning of the pixels of the CCD can be useful if you are just interesting in mapping the intensity of one spectral band. For example, if you have a detector with 1024 pixels along the spectral direction, you can choose to read all the pixels and then the spectrum will be composed of 1024 points. If you don't need spectral resolution, you can choose to work, for example, with 200 data points: the signal from 5 pixels is summed up and gives rise to only one data point. Of course, 1024/200 gives not an integer result and some pixels of the CCD will not be displayed.
Time
There are two types of boxes, the first one is the number of measurements required and the second one is the time interval between them. Be careful, because the acquisition time is included in the interval of time between two measurements.
Z
As for the time dimension there are two boxes, in the first one you introduce the number of measures and in the second box you introduce the interval between the measures. This option can be automatic with a piezo, if you have no piezo, the program stops, asks you to move the focus and start again.
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But you can do more...
Time There are two types of boxes, In the first one you select the beginning and the end of the analysis. In the second one you choose the time interval between them. The choice of the time interval between the measurements is introduced as a function of the number of the measure t . A value: 30 * t will produce a spectrum each 30 seconds, 60 * t each minute. You can introduce more complex timing like log (a*t), exp (a*t) or a*t*t where a is a constant. Depth As for the time dimension there are two boxes, in the first one you introduce the beginning and the end of the analysis and in the second box you introduce the interval between the measures which is a function of the number of the measure z. This option can be automatic with a piezo, if you have no piezo, the program stops, asks you to move the focus and start again. Y You introduce the beginning and the end of the analysis. And you can choose to have a constant size or a constant step between the measurements
X
You introduce the beginning and the end of the analysis. And you can choose to have a constant size or a constant step between the measurements
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Icon 39 Data size
Icon 40
: Acquisition parameters
Spike removing: This option is active only when there is more than one accumulation; it is recommended to give 3 or more accumulations to have a good spike removing. X scanning: Select laser scanning or point by point XY table imaging. Of course in mode Scanner the table will be used to scan the Y direction while the laser will scan the X direction. Scanning area: Cursor or manual - Cursor: The size of the image is selected with the cursor in the video image. - Manual: The upper left corner and the right bottom corner are selected moving the XY table. If you choose this option, the software will ask you to move the table in the appropriate position and validate.
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Refresh time The PC can not record and display the data of a spectral image at the same time. It must stop the recording in order to display the collected data, you can choose to stop periodically the recording and display the data by selecting a refresh time. For the images, to reduce the total time of acquisition procedure, it is better to choose a refresh time equal or higher than 10 minutes. For example, if the refresh time is 300, it means that each 300 seconds, the recording of a spectral image will stop, the display of data is refreshed on the screen, and the recording start again. Autofocus - Autofocus offset This system is an autofocusing of the microscope stage to enable systematic measurements on a device at different operating points. You can choose to add an offset to your autofocus, for example if you want to take a measurement at 2 m in depth choose an offset of 2 This option is active if you choose in the file Hardware.dat: PresentAutofocus = 2 Confocal value You can choose to have : - The intensity in function of the position of the point, if you have chosen confocal value = Intens. or - The z value where the intensity of the signal is maximum, if you have chosen confocal value = Depth Icon 41 Detector parameters
This option permits to select the stripe of the CCD used for single spectrum readout, or the region of the CCD used in image mode. The choice of the region of CCD used for measure allows you to limit the spectral domain of interest and to avoid to read regions without signal that would only increase the readout noise. Usually you have not to change these parameters (settled at DILOR) unless you use the fiber optic entrance. In this case the interesting detector area will be substantially larger than the one obtained under microscope. (In this picture the example is done with a 256*1024 detector).
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Icon 42 Morphological images
When you record an image of the CCD, you can see the area where the bands are. So, with these different parameters, you can select this area. You are allowed to limit the wave number domain and the spatial domain. Icon 42 Morphological images
Clicking on this icon will display a morphological parameters window. These parameters will allow you to get morphological images. To have access to this feature, you have to take a Video image and choose the rectangular cursor in this image. The three first lines allows you to take morphological images in function of the depth. In the First Z and Last Z fields, enter the beginning and the end of your depth profile (e.g.: -10 and 10). In the field Size Z, enter the step between two measurements in depth. Size X and Size Y are the number of points you want to analyze in the X and Y direction. Intensity is the number of counts read out from the PMT during the time defined in the Time field. Repeat is the number of total measurement cycles. Single image option allows you to save all of the morphological images depth profile on a file. To start measurement, press on the START button. Icon 43 Calibration
This window is used to calibrate the moving of the XY table and the amplitude of the scanner. 1. First, you have to select the appropriate objective and record a video image of the sample with the laser spot. This one must be attenuated in order to see its position well. The sample should have some sharp features (corners, dots, dust particles etc...). Press the button . Choose the cursor point into the video image, then position the small rectangle on the laser spot. Press SET on the box Centr (x,y) of the Scale window . The calibration of the laser is done, and when you will record a new video image, a color dot
2. 3.
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4.
will indicate the laser position. Now measure, with the XY table, the distance in X and Y between two details of the sample (the values read out from the table display are ten micrometers. So a read out like 675231.2 means 67523.12 micrometers). Choose the rectangular cursor into the video image. Position the two corners of the rectangular cursor onto the selected details of the sample, then introduce in the box Table (dx,dy) the dimensions of the rectangle as measured with the XY table. Press SET on this box. The equivalence between the video camera pixels and the micrometers are now settled. You can check the calibration of the table if you record a spectrum in a selected point of the video image.
5. 6.
You have to do this calibration for each objective Now calibrate the scanner: - Put the laser scanning mode. - Select the OD 4 filter in order to attenuate the laser - Select the horizontal line cursor on the video image with the length approximately 10% less than the scanned line and center it on the laser spot. - Open the window SCALE . Press SET on the box Scanner (l,r) . You can check the calibration of the scanner doing an image of the sample. If the calibration is not correct, select the cursor line shorter or longer and press again SET on the box Scanner (l,r) .
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Icon 43 Calibration
Icon 44
Configuration
To program some configurations in function of the system. Follow the different steps below: 1- Choose the type of user. 2- Press the ADD button 3- Select all the parameters in the window Parameters To use one of your programmed configurations, in the window configuration select the desired configuration then press set , the software will automatically change all the parameters. Tip: to delete a configuration, select it and press DEL.
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Icon 45
Scheme
This icon permits you to control all the functions of your system: control: change: Acquisition time Spectrograph position Image Acquisition Grating
Different cameras (A) Laser shutter (B) Filters wheel (C) White lights (D) Different security switches (E)
Spectrum Acquisition
B
Hole aperture Exciting line
A D D E
Icon 46
Stop
Stop of spectra recording. Freeze the video image after pressing the Video button (icon n34) Icon 47 Different types of cursors
Three cursors are available and are selected with the buttons at the right lower corner: - A red single cursor for the wavelength and the intensity readout. - A double green cursor for the readout of the width of a band. - A yellow cursor composed of a continuous line and two dotted lines. The continuous line automatically positions to the maximum of the band in the neighborhood of the mouse arrow and the two dotted lines position at the half maximum of the band.
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Icon 47 Different types of cursors
1.4 Menu Bar Description

The menu bar is located in the upper left hand corner of the screen. The File menu commands: Open: Save: Split: To open a previously saved spectra for display. Allows a spectra to be saved for later retrieval. Allows to decompose a spectral image as a set of spectra (you can use this option before or after the recording of the image, when you choose this option, the software asks you a name for the spectra and will increment it by a number for each spectrum) Delete: Delete the active object of the active window. Print: It allows the output to a printer Printer set-up: Printer parameters selection (see the figure below) Exit: Quit and close the LabSpec application.
configuration of the print page. You can choose to have a colored or a black and white printing You can choose the orientation of your print page... To print a spectrum, select the spectrum to print (it must be alone in the window, if not it will print all the object of the active window) then to insert it in your print page press the icon . To introduce the parameters in your print page, press the icon , select the parameters and then press OK.
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The Edit menu commands: Copy Text: Copy picture: Copy data: Paste Text: Paste picture: Paste Data: Copy the text or the parameters that have been selected in the print page. Copy the contents of the active window Copy the data of the active object Allows the ability to paste text in the print page Allows the ability to paste a picture in the print page Allows the ability to paste a spectrum in the Spectrum window This function can be useful to keep an object before correction operations, if these modifications are not used we just have to recall the original object with this option.
The Format menu commands: VIEW DISPLAY: : Spectra in continuous line : Spectra in dotted line : Spectra represented in full surface : Plane surface : Contour line : 3D representation BEHAVIOUR For the spectra: : Active spectrum alone. : All the spectra overlapped. : All spectra in separate smaller windows. : All the spectra in the different points of the spectral image. For the images : : Active image alone. : All the images overlapped in the true color palette. : All the images in separate smaller windows. : All slice images.
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SUPERPOSITION For the spectra: : Only limited spectra are shown (min. and max.) with filled space between them. : All the spectra are overlapped in the window (it could take long time when the PC displays the collected data) : Only a part of the spectra is shown to make displaying time less than 1-3 seconds. : 25% of the spectra are shown. For the images: : Unsmoothed display. : Smooth along lines. : Smooth along columns. : Smooth. IMPOSITION These icons correspond to the different forms of overlapping the recorded spectra or spectral images on the video images. a : Off mode. b : It gives you the points where spectra were recorded on the video image. c : It is the same as b but it also displays the names of the spectra. d : It gives the rectangle where spectral images are recorded. e : The same as d but it also gives the names. f : It gives you the superimposition of spectral images on the video image.
COLORS You are allowed to put or eliminate a coordinate axis (wave number, intensity, X or Y) or put a label on an axis (for example: wavenumber (cm-1): select with an X the axes or labels that you need. The expressions for the labels can be changed You can also change the color of a spectra or of the background
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3D MODE:
You can represent an array of spectra (or a 2D image) like a 3D profile. You are allowed to choose the drawing style. You come back to the original display by pressing the icon display option (menu VIEW of the
This option can be used after 3D mode option . It permits you to see the 3D profile according to different angles.
SCALE - Comm: Common scale. It puts the maxima of the spectra at the same scale. - Incr: Incremental scale. It automatically changes the scale when the limits are expanded. - Freeze : Freeze scale. It freezes the auto-scaling. - Auto: Auto-scale.
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The object menu command: Full list of the objects contained in a window (for example, list of the spectra contained in a spectral window).
The Option menu commands: Multi: This procedure selects all the objects that are on the screen and permits to make an operation to all these objects. For example, you have three spectra on your window, and you want to add the same constant to all these spectra, activate the option multi , the addition will be done on the three spectra. If the option multi is not active, you will have to do this operation for each spectrum. nm-cm-1: choose between nm and cm-1 units.
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Auto save:
1. 1.1
The Auto save option You can choose the directory where the spectra will be recorded: You can choose a name
You can choose to increment the name by the day, the month and the year. BE CAREFUL TO HAVE NO MORE THAN 8 CHARACTERS 1.2 You can choose the files where the spectra will be recorded:
You can choose a name
You can choose to increment the name by number, hour and minutes of the acquisition. BE CAREFUL TO HAVE NO MORE THAN 8 CHARACTERS. 1.3 You can choose the format of the files:
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2.
You have two choices:
2.1 You can choose to work with the Auto Save and Auto Repeat options: For that you choose the delay between two acquisitions (ex: 10 seconds). When you press the icon the software takes an acquisition and record it each 10 seconds. 2.2 You can choose to work only with the Auto Save option: the software takes an acquisition, stop and record it.
- When you press the icon
- When you press the icon (that you use to do spectral imaging, time scanning...) the software take a spectral image and at the end of the acquisition will record it.
BE CAREFUL TO CHOOSE THE TSF FORMAT FOR THE SPECTRAL IMAGES.
The Window menu commands Reorganize: This option permits to re-organize the windows. And permits to select one of the windows that are on the screen (of course, you can select a window with the mouse).
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1.5 Tips section

This section develops some specific practical procedures.
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How to acquire a spectrum?
Tip1: How to acquire a spectrum?
1.
Select the appropriate grating: - 1800 g/mm for a high resolution - 300 or 600 g/mm to make a one shot spectrum - 1200 g/mm which is infrared optimized. (Version only for NIR) Select the correct grating setting in the software. If you change the grating, do not forget to go to the zero order position ( in the software). This will give the correct calibration for the particular grating you will use. Focus the laser on the sample. This can be done through the video image Set the spectrograph at the right position. Select the confocal hole and the slit aperture. Select the acquisition time and the number of accumulations (this will improve the signal/noise ratio) .
2.
3. 4. 5. 6.
Then you have 2 possibilities: - The icon : is a spectrum adjustment, so it can help you in maximizing the signal (the new spectrum refreshes the old one and so on...). No repeated accumulations or extended spectral ranges are acquired. If you dont save the spectrum that you have recording with the icon automatically deleted as soon as you will record a new spectrum. - If you press the icon it will make a spectrum accumulation and stop. , it will be
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How to record spectra at selected points?
Tip2: How to record spectra at selected points?
For LabRam and LabRam Infinity Systems configured with the motorized XY Stage
With the motorized XY stage. 1. 2. Focus the laser on the sample. Take a video image - Press the icon - To freeze the video image press the icon 3. Choice of the cursor.
You can select different points to analyze on the sample, for that, choose the cursor point and position a small rectangle on each point that you want to analyze with the right button of the mouse. If you want to move the point, drag the box by holding down the left mouse button, if you want to delete a point, press the right button. 4. Choice of the grating
Select between 1800 and 600, 300 or 1200 grating options. If you change the grating do not forget to make a zero order. 5. Select the binning on the box Spectral of the icon (cf. index) .
6. 7.
Select the hole aperture and the slit apertures (for the LabRam System). Select the time of exposure and the number of accumulations Accum . To start the recording, press the icon .
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Confocal mapping
Tip3: Confocal mapping

For LabRam and LabRam Infinity Systems configured with the motorized XY Stage
1. 2. adRecord a video image (icon ) and freeze it (icon c) rectangle
Selection of the cursor Inclined line b- Horizontal line Elliptic e- Polygon
This define the area to be analyzed. 3. Select the parameters for imaging: icon You can select the binning (box Spectral) The number of points (spectra) that you want to record along each line (box X) The number of lines (box Y) Of course if you have chosen the horizontal line cursor the box Y is not active. )
4. Selection of the acquisition parameters. (icon In the box X Scanning: choose table Choose the refresh time of the computer display 5. 6.
Select the hole aperture and the slit apertures (for the LabRam System) and the time of exposure. To start the recording press the icon
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Spectral images using a line scan illumination of the sample
Tip4: Spectral images using a line scan illumination of the sample
FOR LABRAM CONFIGURED WITH LINESCAN In the LabRam: - The stem 2 is pushed. - Replace the pinhole H1 by the field lens. On the electronic control box panel: Switch on the scanner. On the software: 1. 2. 3. Focus the laser on the sample. Record a video image and freeze it with the icon Choice of the cursor: There are two possibilities: Horizontal line and rectangle .
4. Select the parameters of imaging: icon - Select the binning - The number of points that you want to record along each lines (box X) - The number of lines (box Y), of course, if you have chosen the line cursor, the box Y is not active. 5. Select the acquisition parameters: (icon ) In the box Scanning device choose Scanner Select the refresh time. 6. 7. Select the slit and the hole apertures and the exposure time. To start the recording press the icon
NOTE: Do not forget to switch off the scanner, and replace the pinhole H1 once you have finished your linescan measurements.
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Time kinetic recording
Tip5: Time kinetic recording
This option enables you to take a series of spectra or spectral images as a function of time. You follow the same procedure as the one to record point by point spectra or spectral images. But you also have to choose: - In the window Data size (icon ): select the parameter time There are two types of boxes, the first one is the number of measures and the second one is the time interval between them. Please refer to the pages Be careful, because the acquisition time is included in the interval of time between two measures. Press the icon to start the acquisition
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Z scanning
Tip6: Z scanning
1. 2. 3. 4. 5. 6. 7.
Focus the laser on the sample, Take a video image and freeze it with the icon Choose the cursor, Select the time of exposure, Select the binning (icon ), ,
In the window Data size , you select the parameters of the Z scanning. Please refer to the Index section Press the icon to start the acquisition
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Baseline correction
Tip7: Baseline correction
The main procedure is to build up point by point a baseline, to fit at best with the spectrum and then subtract. If you have to subtract the baseline of a spectrum, activate this spectrum. If you have to subtract the baseline of an image, activate the window spectral-image . 1. 2. Verify that the option Ins/Del is active (in the box operations ) Select the type for interpolation: it can be linear or polynomial (in the box type ). For the polynomial interpolation, you can also choose the degree of the polynom (in the box degree ). You can select with the mouse in the spectrum window (validate: left button, delete: right button) the points for the baseline computation , a window that contains an average spec-
3.
Tip: (for the images, when you press the icon trum of the image is opened automatically). 4.
Activate the option attachment , to fit at best the baseline to the spectrum.
Tip: An automatic procedure can also be used for the computation of baseline: button Auto 5. Press the button SUB .
NB: The baseline can be saved as a spectrum: for that press the button CONV
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Peak Fitting
Tip8: Peak fitting
To obtain a good Gaussian/Lorentzian fitting, you should prepare the spectrum from spikes. Tip: Correction of the spikes from an image: The correction is done for each spectrum. If a spectrum of an image presents a spike, remove this spike in the window Single Spectrum , then press (on this window) the button R , it will automatically remove this spike from the image. 1. 2. 3. 4. Zoom and extract (button extract of the icon (or of the spectral image). Press the icon Select the function (button FUNC ), you can choose particular function for each band of the spectrum. Select the position of maxima: - Press the icon - The left button add a peak, the right one delete a peak already introduced. (If you want to delete all the selected peaks, press the button Clear ). Press the button APPROX to initialize the parameters Press the button FIT to start the peak fitting procedure. ) the interesting part of the spectrum
5. 6.
For the images, the procedure is the same as the one for the spectra, but the selection of maxima is done in the window Spectral image and you have to give a long time for the procedure of peak fitting (more than 5 minutes). The results of the peak fitting procedure are in the window Bands . You can save these results when you press the button save (the files format is *.bnd). If you want to print the board that contains the results of the peak fitting, open the window Bands Parameters , do a copy text, open the window Print page (option page setup of the edit menu) and to insert this board, choose the option paste text of the main menu. Moreover, for the images, you can have the mapping of the position, the surface, the amplitude, the width at half maximum of the band: In the window Band parameters press the buttons p (for the position), a (for the amplitude), w, s or g the results are in the window Spectral mapping .
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1000
10
800
20
600
30
400
200
40
0
10
20 Length X (m)
30
40
1320
1325
1330
1333.3 1335
1340
1345
Wavenumbers (cm-1)
Video image
10
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10
20 L th X ( )
30
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10
20 Length X (m)
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40
Mapping of the position of the band White color corresponds to the 1336 position, the black one to the 1332 cm-1 position
Mapping of the full width at half maximum. White color corresponds to a 9 cm-1 FWHM and the black one to a 4 cm-1 FWHM
10
20
30
Overlapped of the FWHM and position mapping. The shift of the band goes with an enlarging of this band
40
10
20 Length X (m)
30
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Models
Tip9: Models
It is the decomposition of each original spectrum of an image as a sum of model spectra. 1. Choose the spectra that will become MODELS , they can be already saved on the disk, or they can be particular spectra that you can extract and save from your spectral image. If the spectrum is a particular one extracted from the image, save it. Do the same thing with the other interesting spectra. Load all the selected spectra and put them overlapped in a window (for that use the option Behavior of the menu view of the FORMAT menu). Activate the first selected spectrum and press the button get. Do the same for all the selected spectra: a window is then created with all the model spectra . In the single spectrum window, there are: - The models with relative intensity. - The experimental spectrum in particular point of the image. - The result of the fitting. You will see in the mapping window additional pictures that correspond to each one of the model . You can overlapped them to see the relative contribution of each of the spectra in a particular point of the image (for that use the option Behavior of the menu view of the FORMAT menu 7. You can save a whole spectral image with the model compounds choosing the tvf format. When you will load this image, the decomposition will automatically appear.
2. 3. 4. 5. 6.
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Icons list
20 Length Y (m)
40
60
video image of the sample: The spectra have been taken inside the rectangle.
80
100 0 20 40 60 80 100 120 140
Length X (m)
With the help of the mapping, we can distinguish three different spectra which are: Red compound
0.8 0.6 0.4 0.2 0.0 0.0 800 900 1000 1100 1200 1300 1400 800 900 1000 1100 1200 1300 1400
0.8 0.6 0.4 0.2
Green compound
0.8 0.6 0.4 0.2 0.0
Blue compound
800
900
1000
1100
1200
1300
1400
We can have the mapping of the relative contribution of each compounds in the analyzed sample:
Red compound Blue compound
20 Length Y (m) 40 60 80
Green compound
20
30
40
50
60
70
Length X (m)
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1.6 LabSpec for the T64000 System

This chapter will only detail the LabSpec specific features related to the T64000 System.
What is the T64000 System? The T64000 System is the most powerful, fully computerized, triple spectrograph/scanning spectrometer system ever produced. Unlike the current generation of Raman spectrographs in which spectrometer control and data acquisition are independent functions, requiring different software and hardware, the T64000 System has an integrated software and hardware package which combines all functions in a simplified, easy-to-use fashion. The package contains a large number of security and validity tests, which are only possible with the complete integration of all functions. Conceived initially for the demanding performance of Raman spectroscopy, the T64000 is also superbly adapted to most other spectroscopic techniques. As the software allows the T64000 System to be used in wavelength and wavenumber units, other spectroscopic techniques such as fluorescence, luminescence and absorption/transmission can also be easily performed on both macro and micro samples. The ability to use the T64000 as a single or triple (with subtractive or additive premonochromator stages) spectrometer with either standard scanning or multichannel acquisition of data, permits the user to optimize the spectrometer to the spectral and sampling requirements. This flexibility is achieved without having to disturb the sample under investigation and is completely automated. For industrial research or analytical laboratories the T64000 System is the safest, easiest to operate, and most flexible spectro-analytical system available.
Why a dedicated User Guide Chapter? The LabSpec software must be installed and setup correctly to manage up to 14 Stepper motors, 15 DC motors and 4 electromagnets.
T64000 System and Labspec Software installation The T64000 System and the LabSpec software must be installed and started up by DilorJobin Yvon-Spex or an approved representative. Notice: The T64000 System starting recommendations are described in the T64000 User Manual; Part Number: 31 087 018.
What are the new features that handle the LabSpec? The LabSpec Software now handles the features related to the T64000 Triple Raman Scanning/Spectrograph spectrometer. The new features only concern the additional devices mounted on the System and then do not change the use of the existing LabSpec software.
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T64000 Configuration Setup
Click here to setup configuration
The figure above shows the icon which allows the user to setup the T64000 configuration. The following screen will then be displayed.
First Stage Entrance The default choice is Axial especially if the Microscope or the Macrosample compartment is used. The Lateral choice is an option for specific needs required by the user. Analysis mode The Micro mode concerns the use of the Microanalysis System, the Macro mode concerns the use of the Macroanalysis System. Both are options. the selected option must be present on the system.
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Spectrograph
The T64000 is a triple stage spectrometer. Therefore, the last stage can be used alone (Single) for some specific applications.
Premonochromator The Premonochromator includes the first two stages. By default these stages are configured as subtractive. An additive option can be added. See the hardware user manual for details. Second/Third Stage The link on the exit of the premonochromator and the entrance of the third stage can be direct (default) or external (option). That means that the exit beam can be adapted for a specific application (e.g. Fluorescence option) then analyzed with the third stage. Detection System Select the detector you want to use: Multichannel (CCD) or Monochannel (Single channel PMT or IR detectors). The laser shutter is located before the Microanalysis or Macroanalysis System. Usually, to prepare an analysis session, the laser beam must be present in the microscope or in the macro-sample compartment. With some type of samples, the laser beam can modify or destroy a sample. For these reasons, two choices are available: Always open maintains the shutter off and Open during acquisition opens this shutter only during the acquisition (adapted for laser sensitive samples). Clicking on this button will reinitialize all the commutations listed in this screen plus the grating turret. Validate the selected choices.
Laser Shutter
Initialization
OK
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Monochannel/Multichannel Setup
The previous chapter explains how to select the Monochannel or Multichannel detector for the System which includes both of these detectors. Default, on all T64000 Systems, the Monochannel (PMT) detector is mounted on the third stage axial exit and the Multichannel (CCD) detector is mounted on the top exit. Once the LabSpec software is running, the bottom left screen shows the following setup screen, specifically dedicated to the T64000 System. The use of the displayed parameters is slightly different depending on the chosen mode: Monochannel or Multichannel. Wavelength calibration screen
Close the current slit List of slits Maximal open slit Slit calibration
Double moving Single moving
Premonochromator setup
Spectrometer setup
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How to setup a Monochannel PMT (PhotoMultiplier Tube) detector?

On the figure page 70, the bottom left bar includes two new sections fitted for the T64000 System: the Premono(chromator) and Spectr(ometer) sections. From these sections, the following setup can be performed: Single or Double moving: Double moving means that the Premonochromator and the Spectrometer scan together. Single moving means that the Premonochromator is independent from the Spectrometer.
Security and Optimization Spectrograph Security The figure page 70 shows the Security and Optimization screen. The Monochannel mode is concerned only by the spectrograph security. The choices are Enable (default), Inform and Disable. The Enable choice activates a laser shutter if the Spectrograph scans near to the laser wavelength. The security value (entered on the bottom of the screen) defines the security area. The default Enable choice is highly recommended. The Inform choice is the same as Enable but a pop-up screen will clearly inform the user. The Disable choice allows the user to scan up to laser wavelength (the detector could be damaged). Calibration screen: This feature allows the user to precisely calibrate the premonochromator and the Spectrometer. The complete calibration procedure is described in the Monochannel Mode Calibration chapter.
Monochannel Security Value Enter a value which defines the safety area from the laser beam.
How to setup a Multichannel CCD (Charge Coupled Device) detector?

The procedure below explains how to setup the Multichannel (CCD) Detector. Security and Optimization The figure page 70 shows the Security and Optimization screen. Spectrograph security The Spectrograph security Enable choice activates a laser shutter if the Spectrograph scans from the Stokes to anti-Stokes lines. If a position near to the laser wavelength is chosen, the software calculates the coverage on the CCD detector. If this coverage touches the safety area, the wavelength or wavenumber (central) will be re-calculated; the shutter will not be activated. The Inform choice will display a pop-up screen every time the safety area is touched.
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The Disable choice is only for those who want to manually explore the near-to-laser area and in this case TAKE CARE NOT TO DESTROY THE CCD DETECTOR. First Intermediate Slit optimization (Subtractive) The First Intermediate Slit performs a filtering function on the spectrum to suppress the wavelengths interferences. According to the used gratings, the slit width adapts the pass-band to cover the current CCD Detector. Please note that a spectrum can not completely cover the CCD Detector if different gratings type are selected; in this case, the complete opening of the intermediate slit cannot cover the CCD Detector width. The choices are Enable (default), Inform and Disable. Using a Multichannel CCD Detector, the default Enable choice is highly recommended. The Inform choice is the same as Enable but a pop-up screen will clearly inform the user. The Disable choice allows the user to manually set the first Intermediate slit. Second Intermediate Slit optimization (Additive) Using the additive Mode, the Second Intermediate Slit performs a spectrum filtering. The Enable choice will optimized the slit width according to the selected wavelength or wavenumber. The Disable choice will allows the user to manually set the slit width. Premonochromator Optimization (Subtraction) Usually if the Premonochromator Optimization (for subtractive mode) is enable or disable the action is the same: the Premonochromator moves the position with the Spectrometer. Exception: the Optimization is performed only in this case For specific choices of gratings and if the first Intermediate Slit cannot optimize to cover the total CCD area, the wavelength or wavenumber position of the Premonochromator will slightly move to shift the spectrum to the first pixel of the CCD (if the laser beam is located to the left of the spectrum). The spectrum will be shifted to the right if the laser beam is located on the right. Multichannel Security Value Enter a value which defines the safety area from the laser beam. Once the LabSpec software is running with the Multichannel option selected (see previous page), select the Multichannel detector setup screen by clicking on the following icon:
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CCD Detector Setup The following CCD Detector Setup screen will be displayed:
This option allows the user to select the stripe of the used CCD for single spectrum readout, or the window of the CCD used in image mode. The choice of the region of the CCD used for measure allows you to limit the spectral domain of interest and to avoid reading regions without signal that would only increase the readout and dark noises.
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This following figure shows an example of a 256 x 1024 detector).
When you acquire a CCD image, the useful area will be clearly seen. So, with the parameters of the Detector setup, you can select this area. You are allowed to limit the spectral domain and the spatial domain.
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Monochannel Mode Calibration

The following procedures will explain how to calibrate the System using a monochannel (PMT or IR) detector.
First Time Calibration Usually, the T64000 System and the LabSpec software have been installed and started up by Dilor-Jobin Yvon-Spex or an approved representative. Then, the first time calibration has been already performed.
Calibration troubleshooting The troubleshooting calibration can occur if, during a scanning, the power supply shuts down or if a link fails somewhere. The Premonochromator and Spectrometer position fields will ??? show . Click on the calibration button of the Spectrometer and enter the Counter Value.
Spectrometer calibration fine tune Preliminary The T64000 System can be perfectly calibrated using a known peak. This adjustment can be done in accordance with the following limitations: 1. The calibration will be perfectly reached on the wavelength or wavenumber peak used for the correction. For this reason, try to choose a calibration peak near to your working region. The resulting calibration offset will be saved (if close to the counter wavelength or wavenumber value). Each grating has its own value but NOT if you change the detector, for example the Multichannel. In other words, if you change the detector or choose the Multichannel, verify and eventually create a new calibration offset (the procedure is explained below).
2.
Procedure 1. 2. 3. Run the LabSpec software, From the Commutations screen (see page 68) select the Monochannel Detection System, Select the Monochannel working session by clicking on Monochannel icon ,
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4.
On the Monochannel dedicated icon bar, click on the RTD icon. The RTD will start. Running Acquisition
Monochannel Parameters
Real Time Display
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5.
Monochannel Parameters: The Monochannel parameters allows the user to prpare the acquisition parameters. Follow the instructions describe on the screen below:
Enter here the name of the new preset Select the acquisition channel used by the SPECTRALINK controller Number of total acquisition cycles
Enter region parameters
add a region Enter the PMT high voltage working value Enter the hardware acquisition gain of the channel 2 (if valid)
Select the hardware acquisition gain (1,10,100) of the channel 1 (if the channel is valid)
Enter the safety intensity limit
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6.
Real Time Display: During the RTD acquisition, the Monochannel icon bar is replaced by the RTD Control Panel (see below)
Left/Right scanning toggle buttons Integration Time Increment
Current position Continuous scanning Stop RTD mode Start Time Step by step scanning
7.
Using the RTD Control Panel shown above, scan the spectrometer to the chosen reference peak. The current cursor is represented as a vertical red line located on the screen. When the reference peak is founded, stop the scanning (icon located on the upper right screen), choose the step by step scanning mode and click on the reverse direction scanning up to the highest intensity level of the reference peak (the intensity level is displayed on the right bottom bar of the screen). Stop the scanning at this position. Then do not change the position and follow the next step. The figure located on the next page (page 80) shows the general LabSpec monochannel screen during the reference peak search step.
8. 9.
Click on the close button of the RTD Control Panel, then click on the Close button of the Monochannel icon bar. Running Acquisition: Once the Monochannel Parameters have been correctly setup and once confirmation has been performed with the Real Time Display feature, you can run the acquisition.
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10. Click on the Spectrometer Calibration button to display the calibration screen then:.
A. Select the Real Position choice
B. Enter the reference position C. Click OK to validate The spectrometer is then calibrated
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Stop RTD
LabSpec Software
Cursor line
Spectrometer Calibration button
RTD Control Panel

Current Intensity level
80
Multichannel Mode Calibration

The following procedures will explain how to calibrate the System using a multichannel (CCD) detector.
First Time Calibration Usually, the T64000 System and the LabSpec software have been installed and started up by Dilor-Jobin Yvon-Spex or an approved representative. Then, the first time calibration has been already performed.
Calibration troubleshooting The troubleshooting calibration can occur if, during a scanning, the power supply shuts down or if a link fails somewhere. The Premonochromator and Spectrometer wavelength or wave??? number fields will show . Click on the calibration button of the Spectrometer and enter the Counter Value.
Spectrometer calibration fine tune Preliminary The T64000 System can be perfectly calibrated using a known peak. This adjustment can be done in accordance with the following limitations: 1. 2. The calibration will be perfectly reached on the wavelength peak used for the correction. For this reason, try to choose a calibration peak near to your working region. The resulting calibration offset will be saved (if close to the counter wavelength or wavenumber value). Each grating has its own value but NOT if you change the detector, for example the Monochannel. In other word, if you change the detector or choose the Monochannel, verify and eventually create a new calibration offset (the procedure is explained below).
Procedure 1. 2. 3. Run the LabSpec software, From the Commutations screen (see page 68) select the Multichannel Detection System, Click on the Multichannel calibration icon to start the calibration mode lowing screen will be displayed: . The fol-
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Reference peak
Central pixel
Spectrometer position
How to calibrate the spectrometer with a CCD detector?

The page 73 shows how to setup the CCD matrix. On the screen above, the System performs a Real Time acquisition with this additional important parameter: the central pixel of the CCD is permanently displayed. To calibrate the spectrometer, follow the procedure: 1. 2. 3. Choose a known reference peak and enter the reference position (see above), If the spectrometer is not too de-calibrated, you should be able to see on the screen this reference peak. Try, by entering a new position value, to center the reference peak on the central pixel. This could be performed by successive estimations. Zooming the central area will increase the calibration setting. Once the reference peak is perfectly set on the central pixel, click on the Calibration button of the Spectrometer panel. Then follow the procedure described below:
4.
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A. Select the Real Position choice
B. Enter the reference wavelength C. Click OK to validate The spectrometer is then calibrated
o
Premonochromator calibration and optimization (Subtractive mode)

The T64000 System is composed of three stages: the first two stages are called Premonochromator, the last stage is the Spectrometer. By default, the Premonochromator stages are configured as a subtractive optical design. In this case, the Premonochromator performs a filtering function. If the highest results are required, a Premonochromator calibration is necessary to perform a band-pass on a working area. The procedure described below will show how to optimize a calibration. The goal of this procedure is to close as much as possible the intermediate slit while the reference peak stays visible on the center of the CCD detector. 1. 2. 3. 4. Calibrate the Spectrometer using the procedure described on page 81; at the end of this procedure, the reference peak is displayed at the center of the CCD detector, From the Security and Optimization screen (see page 70), choose Disable the Premonochromator and First Slit optimization, From the Premonochromator Control Panel click on the Single Moving choice, On the same Control Panel, click on the following button and select the First Intermediate Slit. Click on the CCD Calibration icon to display the slits list
5. 6.
to run the calibration Real Time acquisition,
In the slit width field, enter a smaller value than displayed (about 10% less) and verify that the peak is always visible and centered on the CCD. Continue to reduce the interme-
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diate slit. If the spectrum disappears, that mean that the position (Premonochromator) is not centered on the beam path. To re-center the filter, enter a new position value in the Premonochromator field, more or less you have to try, or slightly open the intermediate slit to re-display the spectrum. This is a step by step procedure, and once reached the minimum slit width value, you know that the premonochromator is optimally adjusted and the Acquisition session can be now performed. Please refer to the Training Course Manual for detailed explanations.
Control Panel features
3 4 5
o
10
11
1. Available slits selection list 2. Current slit calibration 3. Single moving (Premonochromator and spectrometer are independent) or double moving mode switch. 4. Premonochromator calibration 5. Security and Optimization modes 6. Spectrometer calibration 7. Close the current slit 8. Slit width field 9. Open the current slit 10. Premonochromator position field 11. Spectrometer wavelength field
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Interactive Index
Symbols
*Const 20 +Const 20
Numerics
3D MODE 50
A
Acquisition panel 12 Acquisition parameters 41 Add a spectrum 25 additive 69 All spectra window 38 Analysis mode 68 Apodisation 29 APPROX 32 Attachment 21 Auto Repeat 53 Auto save option 52 Autofocus 42
Deconvolution 35 Definition of the function 18 Delete the object in the window 14 Depth 40 Description of the different icons 13 detection of the peak 30 detector 39 Detector parameters 42 Detector setup (T64000) 74 Deviation 21 Deviation factor 33 DFT 29 Dilor format 15 Double moving 71
E
Edit, Copy data 48 Edit, Copy picture 48 Edit, Copy Text 48 Edit, Paste Data 48 Edit, Paste picture 48 Edit, Paste Text 48 Exchange a spectrum 24 Exit LabSpec 47 Extended Tiff 15, 16 Extract procedure 35 Extract profile 25 Extracted spectrum 34
B
Baseline correction 19 Baseline correction example 62 binning 39, 57, 61
C
Calibration (spectrometer, monochannel) 79 Calibration (XY stage) 43 CCD detector calibration (T64000) 82 CCD image adjustment 37 CCD Matrix setup (T64000) 73 Color Tiff 16 Commutations screen 75 Compressed Tiff 15, 16 Configuration (system) 45 confocal hole 56 Confocal mapping 58 Confocal value 42 Constant for addition 18 Constant for multiplication 18 Correction 21 cursor for working 13 Cursor normalization 16 cursors (Red, Green, Blue) 35
F
FALSE 33 File, Delete 47 File, open 47 File, Print 47 File, Save 47 File, Split 47 Filter 12 Filtering 26 Fit peak width 14 Fitting (peak) 63 Focusing the laser 57 Format, BEHAVIOUR 48 Format, COLORS 49 Format, IMPOSITION 49 Format, SUPERPOSITION 49 Format, VIEW 48 Formats (Image) 16 formats for spectra saving 15 Fourier transform (filtering) 29
D
Data size 38 Decomposition 35
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Index - 85
Index
G
Gaussian 32 Gaussian/Lorentzian 32 grating (choice) 57 GRAY 33 Green/blue ratio 35
Morphological images 43 Move peak maxima 14 MUL, SUB, ADD, DIV 22 Multi window 17 Multichannel Mode Calibration 81 multiply 14
H
Hole 12 hole aperture 12 Horizontal shift 13 How to acquire a spectrum 56 How to record spectra at selected points? 57
N
Normalization 16, 22 number of accumulations 12
O
object 51 offset (autofocus) 42 Operations between two spectra 18 Operations for the baseline 20 Option menu commands 51
I
IFT 29 Information about object 16 Initialization 69 Ins/Del 20 Insert a spectrum 23 Integral calculation 33 Intensity adjustment 13 inverse Fourier transform 29
P
Palette window 33 peak detection 30 Peak elimination 13 Peak fitting 30, 63 PeakElm 27 Polynomial filtering 28 Position of the spectrograph 12 Premonochromator calibration (T64000) 83 Premonochromator calibration and optimization 83 Print page 16 printer page configuration 47 Printer set-up 47 Profile 23
K
Kinetic 60
L
Label of the peak 14 LabRam 57, 58, 59 LabRam Infinity 57, 58 Laser 12 laser 56 Laser Shutter 69 Line scan illumination 59 Load 14 Lorentzian 32
Q
Quit LabSpec 47
R M
Manual addition 13 Mathematical filtering 29 Mathematical processing 17 Menu Bar Description 47 Model 36 model 65 Models 65 Monochannel Mode Calibration 75 Monochannel/Multichannel Setup 70 Raman/Fluo 35 Recording time 12 Refresh time 42 Removing spectrum 23 Reorganize 53 RTD acquisition (T64000) 78
S
save a TV image 15 save spectra 15 Save the active object 14 scale of a spectrum 13
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Index - 86
Index
scanner calibration 44 Scanning area 41 Scanning device 59 Scheme 46 Second/Third Stage 69 Security and Optimization (T64000) 71 setup Multichannel 71 Shape correction 13 Shift 13 Single moving 71 slit apertures 57 Spectra Calc format 15 Spectral 39 Spectral images recording 37 Spectral mapping 35 Spectral mapping window 38 spectrograph setup 56 Spectrometer calibration (monochannel) 75 Spectrum adjustment 36 spectrum adjustment 56 Spectrum correction 22 Spectrum recording 37 Spike removing 41 Standard Tiff 16 Stop 46 subtractive 69 Summa 35 system configuration 45
V
Vertical shift 13 Video image 36 video image 57
X
X scanning 41
Z
Z scanning 61 ZERO 22 Zoom 13, 20, 34
T
T64000 Configuration Setup 68 T64000 Macro-sample 68 T64000 Microscope 68 T64000 Premonochromator 69 T64000 spectrometer 69 T64000 System 67 Text format 15, 16 thr(%) 21 Threshold 21 TIME 32 Time 12, 39, 40 Time kinetic recording 60 TRUE 34 TSF format 53 types of cursors 46
U
units 51
LabSpec Software
Index - 87

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About the LabSpec User Guide

Icons list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Menu Bar Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

1.1 Icons list

1.2 Control panel

Icon 1 Standard cursor for working

1.3 Description of the panel icons

Move the abscissa scale of the spectrum Icon 6 Vertical shift

Move the ordinate scale of the spectrum Icon 7 Intensity adjustment

Move the scale of a spectrum

Automatic scaling optimization of a spectrum or an image. Icon 19 Print page

Icon 22 Mathematical processing

Multi-Window automatic spectra gluing

Icon 22 Mathematical processing

Data sliding bar Load customized filtering Launch filtering

Here below is the example of a 3-dimension filtering screen:

General: How to perform a filtering ?

Fourier transform (filtering)

Icon 29 Integral calculation

Spectral mapping procedure

Icon 37 CCD image adjustment

- Elliptic and polygon

After the acquisition three windows will be opened

Icon 39 Data size

But you can do more...

Icon 39 Data size

Icon 42 Morphological images

Icon 47 Different types of cursors

1.4 Menu Bar Description

Icon 47 Different types of cursors

Icon 47 Different types of cursors

You can choose a name

Icon 47 Different types of cursors

You have two choices:

- When you press the icon

BE CAREFUL TO CHOOSE THE TSF FORMAT FOR THE SPECTRAL IMAGES.

Icon 47 Different types of cursors

1.5 Tips section

How to acquire a spectrum?

Tip1: How to acquire a spectrum?

Icon 47 Different types of cursors

How to record spectra at selected points?

Tip2: How to record spectra at selected points?

Tip3: Confocal mapping

Selection of the cursor Inclined line b- Horizontal line Elliptic e- Polygon

Icon 47 Different types of cursors

Spectral images using a line scan illumination of the sample

Tip4: Spectral images using a line scan illumination of the sample

Time kinetic recording

Tip5: Time kinetic recording

Icon 47 Different types of cursors

Tip7: Baseline correction

Icon 47 Different types of cursors

Tip8: Peak fitting

Icon 47 Different types of cursors

100 0 20 40 60 80 100 120 140

0.8 0.6 0.4 0.2

0.8 0.6 0.4 0.2 0.0

1.6 LabSpec for the T64000 System