Stat 6200

Homework #1

This homework concerns a single data set from an animal nutrition experiment. The purpose
of the experiment was to determine the effect of dietary protein source and concentration on the
amount of leucine in the plasma of pigs. Three different protein sources were considered: fish,
soy, and milk. The second factor was the concentration of the protein in the food. Two values
were considered: 9% and 18%. Twenty-four pigs were randomly assigned to the 6 factor level
combinations. Some pigs became ill in the course of the experiment and data from those pigs
was omitted from the final table. Consequently there is some missing data. As best as the
researchers could determine, the illnesses the pigs suffered from were unrelated to the diets they
were taking. The response variable in this experiment is the free plasma leucine level in µg/ml.

Source 9% 18%
Fish 27.8 34.0
23.7 30.6
32.7
33.7

Soy 39.3 42.9

34.8 49.0
29.8 44.4
33.2

Milk 40.6 59.8

31.0 72.1
34.6

1. Write down the means model and full effects model for this design.

2. Using SAS, obtain the general form of the estimable functions for this design, as well as
the Type I, Type II, Type III, and Type IV estimable functions.

3. Letting α1, α2, and α3 denote the effects of the 3 sources of protein, β1 and β2 the 2
concentrations, and γij the interaction terms, use the general form of the estimable
functions to determine which of the following parameter combinations are estimable.

a. γ11 – γ12 – γ21 + γ22.

b. α1 – α2 + β1 – β2 + γ11 – γ22.
c. µ + α1 + β2 + γ12 – 3α3 –3 β1 – 3 γ31.
d. µ + 2α1 + 3 β2 + 2γ12 + γ32 + 2γ22 – γ31 + γ11
4. Using the Type I estimable functions, determine the Type I contrasts that are being tested
by the Type I F-ratios for the two factors and the interaction term.

5. Using the Type II estimable functions, determine the contrasts that are being tested by the
Type II F-ratios for the two factors and the interaction.

6. Using the general form of the estimable functions derive the Type III contrasts for the
interactions and for the CONCENTRATION factor (but not the SOURCE factor). You
may check your answers by looking at the Type III estimable functions and using your
knowledge of how Type III tests are related to the population marginal means, but you
should show me the algebra you did to obtain the Type III contrasts using the general
algorithm discussed in class.

7. Using the Type III sums of squares, carry out an analysis of these data. As usual, your
analysis should include residual diagnostics to determine whether the usual ANOVA
assumptions of homoscedasticity and approximate normality are satisfied,
transformations or deletion of outliers if appropriate, F-ratio tests for the various effects
in the model, and appropriate post hoc means comparisons. Express your conclusions in
the context of the original problem.