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NANO-KOLOID SP. Z O.O.

Nano Koloid introduces a patented, novel manufacturing process of metallic non-ionic nano-particle colloids that have been scientifically proven to display a wide range of anti-microbial and anti-fungal properties. Our innovative and superior technology lays in its mono-crystaline nano-particles (nano flakes) that ensure optimal bio-activity, highest effectiveness, purity, and environment safety. From food and beverage, cosmetics, personal hygiene, sanitation, impregnation, military, and electronics to medicine and agriculture the list of nano-colloid applications is endless.
Superior characteristics of nano-colloids: Non-ionic, mono-crystaline form Chemically pure Chemically stable Highest effectiveness at low concentrations Optimal platelet shape of Nano-particles Made in physical process not from chemicals Prolonged effectiveness even after drying Hypo allergenic Lowest light sensitivity Available in custom formulations Unique Patented Technology

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Among the most popular nano-colloids are: anti-microbial silver, anti-fungal copper, anti-inflammatory/rejuvenating gold, catalytic platinum, rhodium, palladium, their alloys and combinations. Each one of the metals has historically been known to possess beneficial properties. Modern science supports and wideness the use of metallic colloids. The superiority of nano-flakes lays in particle size 2-8 nm, a flat surface and several atoms thickness leading to a flake like crystal structure. Nano-Koloid eco-friendly technology improves the existing and opens the doors to new and more efficient applications of colloidal science. This is because the company manufactures uniquely shaped particles whose size, shape, chemical and physical properties deliver stunning and unmatched results. To understand why nanotechnology has such a wide application for instance in medicine, pharmacy, cosmetology, or agriculture, one ought to understand the structure and its characteristics that enable strong mono-crystals reactivity with often unfriendly microbes such as viruses, bacteria, or in some cases fungi. As an example this analysis will focus on Silver (Ag).

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varis from approximately 2 to 8 nm, with an average value of 3.5 nm. Picture presents magnified crystalline metallic nano-particles (nano-crystallites) using TEM Transmission Electron Microscopy. The silver platelets are so thin, that the graphite substrate membrane is visible through them. Visibility of the rows of the crystalline structure can only be explained by the flat surface and visibility of the carbon substrate show the very thin nature of the particles(3-5 atomic layers).

TECHNICAL INFORMATION ABOUT NANO-FLAKES. The diameter of the particles

TEM pictures of nano-flakes

NANO-KOLOID SP. Z O.O.

5 6 the image of the crystal cell metallic silver. Dif10 fraction X-ray studies show that silver crystals 2 1 form regular, centered surface (English abbreviation: FCC face centered, cubic). In this case, 9 12 11 the 8 atoms of silver are located in the corners of 5 5 6 7 18 the cubes, and 6 on the walls. In crystallography 8 10 13 14 this cube is called Elementary cell. Isolated 1 2 4 3 cubes of elementary metal1cell are Figure 2 Combined two elementary cells 15 19 not encountered in nature. 9 12 8 When elementary cubes combine, they form 7 13 nano-particle or crystallites 14 actually do exist that in nature. Figure 2 shows 4 elementary 3 two cubes
of silver combined with each other. These cubes share 5 of the outer-wall atoms and a total of 23 not 28 atoms.

CUBICAL STRUCTURE. Figure 1 shows

Figure 1 Elementary cell

6 2 2 14 12

11

18 2 2 19 14 12

20 10 21 13 11 13

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1 15 17 8

4 12

17

16 3 3

7 7 23

16 3 3

7 7 23

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THE LARGER THE CLUSTERS THE LOWER BIO-ACTIVITY LEVEL. Lastly, one needs to understand how important the nano-particle structure is as it relates to the level of activity of a given particle. As more elementary cells combine with each other, due to their atom sharing on all the internal walls, the activity of particles is drastically reduced. Atoms locked inside these structures have no impact on the outer environment. The larger the mass the smaller is the biochemical activity level. AT THE SURFACE OF NANO-STRUCTURES INCREASES BIOCHEMICAL ACTIVITY INCREASES. As the ratio of the crystal surface to its mass increases, the number of atoms on
external walls increases and with it increases the biochemical potential of crystals.

PHYSICAL MANUFACTURING PROCESS SUPERIORITY. There are many nano colloids or powders available on the market. Powders do not have optimal shapes so their bio-activity level is lower as compared to Nano-Koloid nano flakes. As for chemically manufactured colloids multiple disadvantages and side effects exist that significantly reduce their quality, safety, and bio-activity. As an example, this article will examine chemical reduction of silver from a silver nitrate solution. Although this process can achieve small particles of metal, their surface is contaminated with reagents and cannot be washed off. A thin layer of reagents decreases bio-activity level of particles and poses threat of chemical contamination difficult to detect in nano-materials. Finally, in some colloids come from utilization of electrolysis. These products clearly have a lower surface to mass ratio and are chemically more active due to their ionic form that poses danger of poisoning.
that are 99.999% pure. This means that only one millionth of a million part of mass may be contaminated. This results with our products being 100,000 times more pure than those coming from chemical processes. NANO-FLAKES OF ALLOYS. Nano-flakes come not only in their pure metal form but also as alloys which are difficult, if not impossible, to achieve in chemical or electrolytic processes. Colloidal alloys can provide customized protection against bacteria and fungi. NANO COLLOIDS IN VARIOUS CARRIERS. Most colloids are manufactured in water. This novel technology allows colloids to be manufactured in a variety of liquid media such as: water, alcohols, oils, and organic solvents.

PURITY OF NANO-FLAKE COLLOIDS. Nano-flake colloids are made with mint quality metals

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SCIENCE BEHIND NANO-COLLOIDS. Nano-Koloid products and technology have been tested by multiple laboratories and scientific institutions such as: Institute of Physics PAS (structure), Department of Chemical Analytic Laboratory of Warsaw University, Warsaw University of Technology (composition), the Potato Institute (efficiency), SGGW Warsaw (impact on the economic development of the poultry), Ita-Test Laboratory test (safety, germicidal and dermatological tests). HOW DOES NANO-SILVER WORK. GENERAL INFORMATION:
Nano-Silver power lays in its unsurpassed ability to destroy bacteria and some fungi. Silver particles block bacterias ability to nourish and excrete waste products. What differentiates silver from antibiotics is that : Microorganisms are not able to create the mechanisms of resistance against Nano-silver. Antibiotics are successful only on a small group of bacterium. Does not interact or interfere with other medications, chemicals, or ingredients. Silver colloid does not work as quickly as bleach or alcohol, but is still much more effective because it does not irritate the skin and lungs, works on a much wider spectrum of bacteria and is long lasting because it works even after it dries out. Illustrations of few bacteriological tests demonstrating antibacterial property of the water silver colloid (0.5PPM)
after treating samples of bacteria after 0 min, 15 min., and 3 hours: Entercoccus hirae ATCC 10541

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Picture 2. Laboratory test bacteriological tests demonstrating antibacterial property of the water silver colloid (0.5PPM) after treating samples of bacteria after 0 min, 15 min., and 3 hours. Nano-Silver power lays in its unsurpassed ability to destroy bacteria and some fungi. Silver particles block bacterias ability to nourish and excrete waste products.

Staphylococcus aureus ATCC 6538P

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Pseudomonas aeuroginosa ATTC 15442

Escherichia coli ATCC 10536

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APPLICATIONS:

Nano-silver has an endless list of uses. The following graph broadly presents industries where nano-silver is being used in production of goods.

FOOD INDUSTRY: Drinking water bio-purification Wine industry -substitute sulfates with nano-silver for health purposes Poultry and egg production- prevent growth of deadly bacteria such as salmonella Vegetable protection-prevent premature spoilage Packaging- create intrinsically safe food containers MEDICINE: Wound care-ingredients or stand alone products ( bandages, band aids, burn wound protection, ointments, ear drops, sore throat spray, ect.) Gum disease (tooth paste and mouth rinse) Disease prevention especially in case of antibiotic resistant strains of bacteria (E-coli, streptococcus, or Mersa, Staphylococus) BUILDING MATERIALS INDUSTRY: Wood protection from mold and bacteria (impregnating solution) Anti-bacterial paint for hospitals and medical facilities PERSONAL HYGIENE: foot care acne solutions non-irritating aftershave intimate care HOUSEHOLD AND MEDICAL SURFACE PROTECTION: Additive to household and car cleaners & disinfectants Additive to professional cleaning substances AGRICULTURE: Animal disease control Protection of plants from bacteria

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COSMETICS INDUSTRY Natural protection of cosmetics from spoiling Additive to deodorants, foot care products, creams, lotions, salon and spa applications MILITARY: Protection from biological weapons Personal Hygiene Food protection Wound care Water bio-purification ELECTRONICS: electrocondutive paints, coatings, and lubricants CHEMICAL INDUSTRY: Platinum catalyst

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