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Ambiguous Genitalia
Maternal history ingestion of drugs, alcohol, or hormonal agents during pregnancy progestational therapy for threatened abortion or androgen therapy for endometriosis Maternal S/S of androgen overproduction, such as acne, deepening of the voice, or hirsutism during pregnancy Family history previous occurrence of genital ambiguity, prior neonatal deaths, consanguinity, and infertility
Physical Examination
Palpable gonads or not Dysmorphic features and multiple anomalies B.P. � 11-OH ase deficiency CAH Areolar or genital hyperpigmentation, R/O CAH Stigmata of Turner syndrome Phallus length: normal penis> 2.5 cm Female clitoris normal < 1 cm Hypospadias � position of urethral meatus 1st degree � glans 2nd degree � shaft 3rd degree � perineum A rectal examination to palpate the uterus The presence of a vagina, vaginal pouch, or urogenital sinus The degree of fusion of labioscrotal folds
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Reference: Holm IA: Ambiguous genitalia in the newborn. In: Emans SJ, Laufer MR, Goldstein DP eds. Pediatric adolescent gynecology. 4th eds. Lippincott-Raven: Philadelphia. 1998:58. Bin-Abbas B, Conte FA, Grumbach MM, Kaplan SL. Congenital hypogonadotropic hypogonadism and micropenis: Effect of testosterone treatment on adult penile size - Why sex reversal is not indicated. J Pediatr 1999;134:579-83. Meyers-Seifer CH, Charest NJ: Diagnosis and management of patients with ambiguous genitalia. Seminars in Perinatology 1992;16:332-339.
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5-reductase 2 gene in patients with 5-reductase deficiency AMH gene in persistent mullerian duct syndrome 8. a therapeutic trial of hCG and testosterone, which take 6-8 weeks hCG (3000 U/M2/dose biweekly for about 5 weeks) Three monthly intramuscular injections of testosterone enanthate, propionate, or cypionate 25-50 mg, should produce lengthening of the penis by 2.00.6 cm, and in this case, the patient is assigned a male gender. 9. genetic counseling -- CAH: autosomal recessive trait androgen insensitivity syndrome: X-linked Reference: Warne GL, Hughes IA: The clinical management of ambiguous genitalia. In: Brook CGD eds. Clinical Paediatric Endocrinology. 3rd ed. 1989. Oxford: Blackwell Science. pp 53-68. Holm IA: Ambiguous genitalia in the newborn. In: Emans SJ, Laufer MR, Goldstein DP eds. Pediatric adolescent gynecology. 4th eds. Lippincott-Raven: Philadelphia. 1998:49-73. Table 4.1 A differential diagnosis based on the presence or absence of the uterus in an infant with ambiguous genitalia Uterus present Genotypic female with CAH Uterus absent Androgen-insensitivity syndrome
Female virilized by transplacental 5-Reductase deficiency androgen or by endogenous tumour Block in testosterone biosynthesis Partial gonadal dysgenesis Primary gonadotrophin deficiency Absence of Leydig cells Drug-induced blockade of androgen action Table 4.2 Steps in establishing the diagnosis in an infant of uncertain sex
Clinical diagnosis 21-Hydroxylase Gonadal dysgenesis Partial androgen deficiency with Y chromosome insensitivity + + Block in testosterone biosynthesis +
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Uterus present
+ -
-/+
Increased skin + pigmentation Sick baby Dysmorphic appearance Investigation Serum 17-OHP Electrolytes Karyotype Testosterone response to hCG Gonadal biopsy Abnormal 46,XX Not indicated +/-
+/-
-/+ -
Normal
Normal
Normal
Normal
Normal
Not indicated
Normal testis (+/Leydig cell hyperplasia) Genital skin fibroblast culture for AR assay
Normal testis
Other
THE KARYOTYPE Table 4.3 Causes of ambiguous genitalia in infants with a 46,XX karyotype Problem Cause Specific examples 21-Hydroxylase deficiency 11-Hydroxylase deficiency 3-Hydroxysteroid dehydrogenase deficiency Androgens crossing the placenta Testis or ovotestis present Partial gonadal dysgenesis
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Virilizing disease in hyperplasia the mother Ovarian tumour Non-androgenic Placental aromatase deficiency
Table 4.4 Causes of ambiguous genitalia in infants with a 46,XY karyotype Problem Insufficient androgen Cause Gonadal dysgenesis Block in testosterone biosynthesis Specific examples SRY gene mutation 17-Ketosteroid reductase deficiency 3-Hydroxysteroid dehydrogenase deficiency Primary Leydig cell hyperplasia Impaired response to Abnormal androgen androgen receptor Unresponsiveness to LH/hCG Partial androgen insensitivity
Deficient conversion 5-Reductase deficiency of testosterone to DHT DHT, dihydrotestosterone, hCG, human chorionic gonadotrophin, LH, luteinizing hormone.
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Figure. Scheme for a standard diagnostic approach to the evaluation of severe hypospadias. Albers N, Ulrichs C, Gliier S, Hiort O, Sinnecker GHG, Mildenberger H, Brodehl J: Etiologic classification of severe hypospadias: Implications for prognosis and management. J Pediatr 1997;131:38692.
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