i AS 5013.

23—2004
ISO 17410:2001
TM
Australian Standard
Food microbiology
AS 5013.23

Method 23: Microbiology of food and animal feeding

stuffs—Horizontal method for the enumeration of
psychrotrophic microorganisms

PREFACE
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This Standard was prepared by the Joint Standards Australia/Standards New Zealand Committee
FT-004, Food Microbiology to supersede AS 1766.2.14:1991, Food microbiology—Examination for
specific organisms, Method 2.14: Psychrotrophic organisms in dairy products.
This Standard is identical with and reproduced from ISO 17410:2001, Microbiology of food and
animal feeding stuffs—Horizontal method for the enumeration of psychrotrophic microorganisms.
This Standard was prepared by the Australian members of the Joint Standards Australia/Standards
New Zealand Committee FT-004. After consultation with stakeholders in both countries, Standards
Australia and Standards New Zealand decided to develop this Standard as an Australian Standard
rather than an Australian/New Zealand Standard.
The objective of this Standard is to specify a method for the enumeration of psychrotrophic
microorganisms by means of the colony-count technique at 6.5°C. The method is applicable to
products intended for human consumption or for animal feeding stuffs.
As this Standard is reproduced from an International Standard, the following applies:
(a) Its number appears on the cover and title page while the International Standard number appears
only on the cover.
(b) In the source text, ‘this International Standard’ should read ‘this Australian Standard’.
(c) A full point substitutes for a comma when referring to a decimal marker.
(d) Substitute ‘mL’ for ‘ml’ wherever it appears.
References to International Standards should be replaced by references to equivalent Australian
Standards as follows:
Reference to International Standard Australian Standard
ISO AS
6887 Microbiology of food and animal 5013 Food microbiology
feeding stuffs—Preparation of test
5013.11.1 Method 11.1: Microbiology of food
samples, initial suspension and
and animal feeding stuff—Preparation
decimal dilutions for microbiological
of test sample, initial suspension and
examination
decimal dilutions for microbiological
6887-1 Part 1: General rules for the
examination—General rules for the
preparation of the initial suspension
preparation of the initial suspension
and decimal dilutions
and decimal dilutions
 ii

ISO AS
7218 Microbiology—General guidance for 5013.14 Method 14: Microbiology of food and
microbiological examination animal feeding stuffs—General rules
for microbiological examination
8261 Milk and milk products—Preparation 5013.17 Method 17: Milk and milk products—
of samples and dilutions for Preparation of samples and dilutions
microbiological examination for microbiological examination
The laboratory should have a clearly defined quality control system to ensure that the apparatus,
culture media, reagents and technique are suitable for the test. The use of positive controls is part of
this system.
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 1

1 Scope
This International Standard specifies a method for the enumeration of psychrotrophic microorganisms by means of
the colony-count technique at 6,5 °C. The method is applicable to products intended for human consumption or for
animal feeding stuffs.

2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of
This is a free 3 page sample. Access the full version at http://infostore.saiglobal.com.

this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent editions of the normative documents indicated below. For
undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC
maintain registers of currently valid International Standards.

ISO 6887-1, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial
suspension and decimal dilutions.1)

ISO 7218, Microbiology of food and animal feeding stuffs — General rules for microbiological examinations.

ISO 8261, Milk and milk products — Preparation of samples and dilutions for microbiological examination.

3 Term and definition

For the purposes of this International Standard, the following term and definition applies.

3.1
psychrotrophic microorganism
bacteria, yeasts and moulds forming countable colonies under the conditions specified in this International
Standard

4 Principle
4.1 Two agar plates are prepared using a solid non-selective culture medium, and using a specified quantity of
the test sample if the initial product is liquid, or using a specified quantity of an initial suspension in the case of
other products. In order to prevent heat stress, surface inoculation is used.

Other pairs of agar plates are prepared, under the same conditions, using decimal dilutions of the test sample or
the initial suspension.

1) Parts 2 to 5 are in preparation, see biliography.

www.standards.com.au  Standards Australia

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AS 5013.23-2004, Food microbiology Microbiology

of food and animal feeding stuffs - Horizontal
method for the enumeration of psychrotrophic
This is a free 3 page sample. Access the full version at http://infostore.saiglobal.com.

microorganisms

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