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biology-honors
mrs. ermi
10 may 2006
laboratory objectives:
1. identify and describe the structure and function of each cell type and tissue type.
4. describe primary and secondary growth and identify the location of each in the plant.
5. relate primary and secondary growth to the growth habit (woody or herbaceous).
6. discuss adaptation of land plants to the terrestrial environment as illustrated by the structure
7. apply your knowledge of plants to the kinds of produce you find in the grocery store.
answers:
a: stems - stems provide support and shape of the plant. they facilitate nutrient transport and
function as a central hub. in addition, the stem often provides a role in storage (starches, etc.) and
photosynthesis.
roots - roots aid water and nutrient absorption and provide anchorage. in addition, roots are a
leaves - leaves function as the primary synthetic organ. in addition, leaves support transpiration
2. q: imagine that you cut each organ---roots, stems, and leaves---in cross section. sketch the
overall shape of that cross section in the margin of the lab manual. remember, you are not
1. q: describe the changes in cell size and structure in the stem tip. begin at the youngest cells at
a: as the smaller cells in the stem tip progress upwards, they become bigger and longer. at the
indeterminate growth. imagine a 200-year-old oak tree, with active meristem producing new
buds, leaves, and stems each year. contrast this with the growth pattern in humans.
a: humans feature determinate growth. eventually, at a certain point, growth almost stops with
only necessary facilitating cell divisions taking place. consistent development is not possible
a. stems
a: xylem; this is in part due to the distinct size of xylem vessels and tracheids.
2. q: what types of cells provide support of the stem? where are these cells located in the stem?
a: sclerenchyma are dead, fibrous, thick-walled cells that provide support to the phloem tissue.
3. q: for the cells described in your preceding answer, how does their observed structure relate to
a: sclerenchyma have thick walls purely purposed towards rigid support for the phloem cells.
the collenchyma cells have cell walls not quite as thick, permitting strength with flexibility. they
are strong and pliable so as to not snap in unfavorable conditions such as heavy winds.
a: xylem transports water and inorganic nutrients upwards in the plant through the roots.
phloem distributes organic compounds throughout the plant in all areas and directions.
5. q: the pith and cortex are made up of parenchyma cells. describe the many functions of these
a: parenchyma serve the primary purpose of storage, and secondarily as photosynthetic and
support cells.
essentially, they are large, commodious organelles (vacuoles) for storage. when filled with water,
their turgor pressure is at maximum and they press on the cell wall; a state known as being
turgid.
6. q: what differences did you observe in the prepared stem sections and your hand sections?
a: the differences in colors and quality lend themselves to the use of staining, agents, and
professional processing.
b. roots
which of the four functions of roots listed in the introduction to this lab do you think is related to
starch storage.
1. q: suggest the advantage of taproot and fibrous roots under different environmental conditions.
a: taproots are suitable for many types of earthy soils, as they penetrate deep into the soil for
anchorage and to absorb nutrients. fibrous roots are suitable for loose, wet soil and aqueous
environments as their large surface area and dispersed, fibrous roots better absorb nutrients while
sacrificing anchorage.
a: not applicable.
3. q: compare the structure and organization of roots and stems. how do these two organs differ?
a: roots possess an internal endodermis to guard the vascular tissue, as well as the casparian strip
as an adjacent cell guard. this structure allows for water retention within the root and inhibits
water loss back into the soil. root hairs on the root increase surface area for nutrient and mineral
absorption. roots have designated vascular tissue in the center, with no piths.
stems possess chlorophyll more often than roots, and possess a ring of vascular tissue systems
around a central pith. shoots and buds are exclusive to the stem as well.
4. q: explain the relationship of structure and function for two structures or cells found only in
roots.
a: root hairs are long and threadlike secondary projections of roots that increase the surface area
the waxy endodermis regulates what enters and exits the central vascular tissues, permitting
greater retention of water and preventing water from returning to the soil.
5. q: note that the epidermis of the roots lacks a cuticle. can you explain why this might be
advantageous?
a: the absence of a waxy cuticle permits a more complete absorption of water and minerals into
the root.
6. q: what is the function of the endodermis? why is the endodermis important to the success of
a: the endodermis regulates the materials that enter and exit from the vascular tissue. the
endodermis allows for land plants to retain and release desired and undesired matter more
effectively. it is primarily used to aid in water retention, forcing water into the vascular tissue and
c. leaves
xylem.
observe the distribution of stomata in the upper and lower epidermis. where are they more
abundant?
a: leaves are organs especially adapted for photosynthesis. the thin blades provide large
comparative surface area for the absorption of light and uptake of carbon dioxide through
stomata.
2. q: provide evidence from your observations of leaf structure to support the hypothesis that
structure and function are related. be specific in your examples.
a: the leaves possess a large surface area for increased absorption of light. in addition, the
stomata and guard cells aid water retention and facilitate carbon dioxide uptake and oxygen
release. the mesophyll provides area for necessary gas exchange and water storage.
3. q: explain the observation that more stomata are found on the lower surface of the leaf than on
the upper.
a: there are more stomata found on the lower surface of the leaf less water is traditionally lost
through lower stomata. carbon dioxide and oxygen are exchanged in this area, making water loss
4. q: explain the differences observed, if any, between the stomata from leaves kept in light and
dark environments.
a: in light, the stomata were open because photosynthesis was occurring. they were turgid
because the influx of k+ ions allowed water to diffuse into the cell and cause the pore to open. in
the dark environment, the stomatal guard cells were limp and flaccid as photosynthesis could not
occur.
a: the phloem after several years forms into a barky material through aging, cell death, and
layering.
2. q: based on your observations of the woody stem, does xylem or phloem provide structural
the xylem, or "wood," essentially provides the bulk of structural support for trees.
4. q: how might the structure of early wood and late wood be related to seasonal conditions and
the function of the cells? think about environmental conditions during the growing season.
a: the early wood is formed when growth is comparatively rapid, hence its given structure within
the woody stem to develop the woody xylem inwards. the late wood is often later into the season,
1. q: what feature of the white potato provided key evidence in deciding the correct plant organ?
a: the leaf buds, or “eyes” distinctly indicate that the white potato is, in fact, a stem.
2. q: based on your knowledge of the root, why do you think roots have been selected so often as
food sources?
a: since roots are often used to stockpile reserves, they are packed with starch and carbohydrates
3. q: what characteristics of sieve-tube structure provides a clue to the role of companion cells?
a: the small pores at the sieve plates with the plasmodesmatal structures lend themselves to the
presence of companion cells. companion cells are integral as a "cellular lifeline," performing
4. q: compare primary and secondary growth. what cells divide to form primary tissue? to form
secondary tissue? can a plant have both primary growth and secondary growth? explain,
a: primary growth is primarily involved with the lateral growth of plants. this growth often
occurs at primary meristems. cambium cells provide secondary growth, or girth. it is possible to
undergo both primary and secondary growth modes simultaneously. trees are ideal examples of
1. q: cells of the epidermis frequently retain a capability for cell division. why is this important?
a: the epidermis serves to protect the plant and to regulate the movement of materials. as a plant
grows or undergoes any type of damage, the ability of the epidermis to divide allows the plant to
2. q: why is the endodermis essential in the root but not in the stem?
a: the endodermis forces water in the root into the xylem while preventing it from returning to
3. q: when lateral roots grow outward from the pericycle, what effect does this have on the cortex
and epidermis? (hint: review the structure of the root and the location of these tissues.)
a: as lateral roots grow outward from the pericycle, the cortex and epidermis are forced to
4. q: the belt buckle of a 20-year old man may be a foot higher than it was when he was 10, but a
nail driven into a 10-year-old tree will be at the same height 10 years later. explain.
a: primary tree growth occurs at the top of the shoot, in the apical meristem. human growth
5. q: explain from a cellular point of view how it is possible to determine the age of a tree.
a: it is possible to determine the pattern of growth within the xylem and phloem to assign an
overall age to a tree. ray parenchyma cells provide clues as well as the xylem “rings.”
6. q: the oldest living organisms on earth are plants. some bristlecone pines are over 4,000 years
old, and a desert creosote bush is known to be 10,000 years old. what special feature of plants
provides for this incredible longevity? how do plants differ from animals in their pattern of
a: plants feature alternation of generations which ensures propagation according to the suitability
7. q: plant cells have cell walls and animal cells do not. how does this difference relate to
a: in plants, water retention is much more important than in animals. the cell wall helps to
prevent water loss. in addition, since most plants are relatively immobile, cell walls bolster plant