‫بسم هللا الرحمن الرحيم‬

GROWTH AND NUTRITION

OF BACTERIA

Prof. Khalifa Sifaw Ghenghesh

Dept. of Medical Microbiology,
Faculty of Medicine, Al-Fateh University
Tripoli - Libya
 Growth: An Orderly Increase in All components of an Organism
– Multiplication >> Binary Fission.

 Measurement of Growth:
– Increase in Numbers
– Increase in Mass

 Batch Culture:
– Growth Curve:
 A Total Count.
 A Viable Count.
1. Lag Phase
2. Log (logarithmic) Phase
3. Stationary Phase
4. Death Phase
GROUTH REQUIREMENTS AND BACTERIAL NUTRITION:
 Suitable Nutrients Must Be Supplied
 Physical Conditions Must Be as Near as Possible for
the Organism Under Consideration

BACTERIAL METABOLISM:
Bacteria Require:
 An Energy Source
 A Carbon Source
 A Nitrogen Source
 Other Inorganic Salts and Accessory Growth Factors.

1. Photo-autotrophs:
2. Chemo- autotrophs
3. Heterotrophs:
OXYGEN REQUIREMENTS AND METABOLISM:
1. Obligate Aerobes
2. Obligate Ananerobes
3. Facultative Anaerobes
4. Microaerophilic Bacteria.

MEDIA FOR BACTERIAL GROWTH:

1. General Laboratory Media:
– Peptone >> Amino acids
– Growth factors and inoorganic salts
– NaCl
2. Synthetic Media
3. Special Media:
– Selective media
– Indicator media
– Enrichment media
 PHYSICAL CONDITIONS
REQUIRED FOR GROWTH

1. Influence of Oxygen and CO:

2. Influence Temperature:
– On Growth:
 Mesophilic bacteria
 Psychrophilic bacteria
 Thermophilic bacteria

– On Viability:
3. Influence of Moisture and Desiccation:
4. Influence of Hydrogen Ion Concentration
(pH):

5. Influence of Light and other radiations:

6. Influence of Osmotic Pressure:

7. Influence of Mechanical and Sonic Stresses: