RESULTS

5.0 RESULTS

5.1 Representative of Agarose gel

5.1.1 Amplification of the STR loci D16S539, D7S820 and D13S317 (SilverSTR® III

marker)

Result of the agarose gel electrophoresis for DNA amplification of the STR

SilverSTR® III marker loci is shown in figure 1. The SilverSTR® III marker loci were

simultaneously amplified. The three loci were amplified properly and all three bands of each

locus can be seen.

Table 6: Size range for the SilverSTR® III loci

STR loci Size range (bp)

D16S539 264 – 304

D7S820 215 – 247

D13S317 165 – 197

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 RESULTS

Figure 1: Amplification of the STR loci D16S539, D7S820 and D13S317

(SilverSTR® III marker).

Lane 1 and 2: grandchild 4

Lane 2 and 3: grandchild 6

Lane 4: positive control, presence of bands show successful of amplification

Lane 5: negative control, absence of amplified band.

Lane 6: 100bp molecular weight ladder

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 RESULTS

5.2 Representative of Polyacrylamide gel

5.2.1 Representative of Polyacrylamide gel for SilverSTR® III marker

Figure 2: Representative of Polyacrylamide of SilverSTR® III

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 RESULTS

Lane 1: Silver STR III ladder

Lane 2: DNA marker

Lane 3: sample 1 (grandfather)

Lane 4: sample 2 (grandchild 1)

Lane 5: sample 3 (grandchild 2)

Lane 6: sample 4 (grandchild 3)

Lane 7: sample 5 (grandchild 4)

Lane 8: sample 6 (grandchild 5)

Lane 9: sample 7 (grandchild 6)

Lane 10: positive control

Lane 11: Silver STR III ladder

5.3 Case study

From figure 1, two samples were chosen for DNA amplification on 1% agarose gel

for SilverSTR® III multiplex loci. The result shows a successful amplification with three

overlapping loci based on the expected sizes. All samples were then subjected to DNA typing

that was performed using 4 % denaturing polyacrylamide gel electrophoresis. The result of

amplification of the loci is shown in Figure 2. The allele classification was used in comparison

with the allelic ladder provided with the STR marker. Direct comparison between the allelic

ladders and amplified samples of the same locus allows for assignment of alleles. The

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 RESULTS

genotype of the individuals should be determined according to the multiplex primer pairs.

However, the lanes of ladder show faint bands. The bands for all three loci are fuzzy while

there is a locus that does not show bands. The D7S820 does not show any migration of the

samples and no bands can be seen. Thus, the genotype of the samples cannot be determined.

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