mWasabi GFP Fusion Expression Vectors

R etroviral infection of cells is an effective means of

stably inserting a gene of interest into a host cell.
The pCHAC retrovirus is successful in infecting most
mammalian cells, including human. These vectors
are suitable for most challenging fluorescent protein
applications.

A llele’s pCHAC fluorescent protein fusion ex-

pression vectors use efficient and unique tech-
nologies to get the best results for our product. The
pCHAC retroviral vector is based on the Moloney
Murine Leukemia Virus (MMLV) and contains LTRs
and packaging signals. These vectors can be used
to express a protein of interest as either an N- or
C-terminal fusion to a fluorescent protein by inserting pCHAC-mWasabi-C
cDNA into the MCS, or for packaging a stand-alone 421-1012 5’LTR
control retrovirus that expresses an FP in infected
2321-3028 mWasabi GFP
cells. Allele uses the brightest FPs, in their respective
3042-3107 MCS
spectrums, to date: mWasabi, mTFP1, LanRFP and
LanYFP. These vectors result in remarkably high titer, 3152-3743 3’ LTR
108 TU/ml and higher. 4018-4657 pUC Region
4807-5667 Ampicilin Resistance
Gene

pCHAC-mWasabi-N
419-1012 5’LTR
2299-2385 mWasabi GFP
2392-3103 MCS
3172-3763 3’ LTR
4038-4677 pUC Region
4827-5687 Ampicilin Resistance
Gene

Box 1 | Product List

FP Fusion Vectors

S afety issues
Retroviral vectors should be han-
dled using NIH BSL-2 safety guide-
pCHAC-mWasabi-C
ABP-PVL-WCNCS01 1µg
lines. For more information, please ABP-PVL-WCNCS10 10µg
see Biosafety in Microbiological and
Biomedical Laboratories <4th edition> pCHAC-mWasabi-N
which is available on the Web sites ABP-PVL-WNNCS01 1µg
of the National Institutes of Health at
ABP-PVL-WNNCS10 10µg
http://bmbl.od.nih.gov.

F or Research Use Only. Not for Diagnostic or Therapeutic Use. Purchase does not include or carry any right to resell or transfer
this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted
use without the express written authorization of Allele Biotech is strictly prohibited

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