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B T C 0 2 3 Course Immunology and Immuno technology

Course Title:
Code:

Weightage
L T P Cr
CA MTE ETE
3 0 2 4
40 15 45
Pre-requisites: Nil
Course Contents:
S.no Topic Approximate No. of
. Lectures
(Scheduled Keeping
total as 7*L)
1. Terminology of immune system; Types of immunity: innate and 2
adaptive; Features of immune response and memory; humoral
and cell mediated immunity.

2. Cells of immune system: lymphoid cells and myeloid cells. 2

3. Organs of immune system. 3

4. Nature of antigen; Factors influencing immunogenicity 1

5. Antibody structure, function and classes. Monoclonal antibodies 3

6 Antigen antibody interaction, affinity and avidity. 2

7 Various types of immunodiffusion and immunoelectrophoretic 3


procedures: Immunoblot, ELISA, RIA, Haemagglutination
assay.
8 MHC, MHC molecules and genes. 2

9 Antigen processing and presentation: Cytosolic and Endocytic 2


pathway.
10 The complement system. 3

11 Introduction to cytokines 2

12 Hypersensitivity and its types. 3

13 Introduction to Autoimmunity, Immunodeficiencies 3

14 Immunity to viruses, bacteria, fungi. 2


15 Tumors 1

16 Graft rejection 2

36
TOTAL

Laboratory Work:

1. To determine blood group of own blood.


To determine total leucocyte count
2. To separate serum from blood.
To determine species of origin.
3. Double Immunodiffusion
4. Immunoelectrophoresis
5. Radial Immunodiffusion
6. Dot ELISA
7. Latex agglutination
8. Isolation of lymphocyte population.
9. Macrophages extraction.

Textbook

1. Kuby immunology, By :- Thomas J. Kindt, Barbara A. Osborne, & Richard A. Goldsby.

Additional Readings:
1. Basic immunology:- Functions and disorders of the immune system. By:- Abul K
Abbas, & Andrew A. Lichtman
2. Immunology, (Lippincotts Illustrated Review) By:- Thao Doan, Roger Melvold,
Susan Viselli, Carl Waltenbaugh.
3. Immunology- By;- Ivan Roitt.

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