Allele Competent Cells

Spreading Beads,Competent
Cells Companion

T he Allele Competent Cells Spreading Beads are offered

to provide convenience and to increase efficiency when
spreading transformed bacterial or yeast cells. These
Box 1 | Product List

beads are manufactured through a special process to give Competent Cells Spreading Beads
consistent shape and diameter to yield optimal plating ABP-CE-CCCSB100 100 plates
efficiencies. Simply pour ~10 beads per 10-cm agar dish, ABP-CE-CCCSB500 500 plates
move side ways for a few times, the transformed cells will be
evenly distributed throughout the plate surface. The rolling
motion of the beads provides gentler spreading force and
the elimination of the flaming step in the traditional bent rod/
spinning disk method ensures more colonies.
Protocol
Packaging: 1. Pipet transformed cells to the center of the plate.
Sterile glass 5 mm beads, ~1,000 beads for 100
plates, packaged in 1 or 5 bottles per order. 2. Add about 10 beads (no need to be accurate) to the plate.
If desired, can prepare beads for multiple plates and stack
them together

Features 3. Move back-and-forth or side ways for about 10-15

seconds, pour off beads.
♦No waste of expensive ethanol or potentially
hazardous flaming, save time and cost 4. Invert plate, incubate in 37°C incubator overnight. The
colonies will appear evenly throughout the plate.
♦ Quick and easy, do as many plates as you
like at once

♦ Gentle spreading, more colonies in most

Notes: _________________________________________
cases
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♦ Even plating, no colonies grouped together
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or on the side of the plate
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♦ Environmentally friendly process using only
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glass beads made out of sand.
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Related Products
 Xtreme DH5α Electrocompetent Cells  Xtreme DH10β Electrocompetent Cells
 Most commonly used E.coli strain for high  Most commonly used E.coli strain for high
efficiency plasmid transformation. efficiency plasmid transformation by electroporation.

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