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Lipoproteins
particles found in plasma that transport lipids including
cholesterol
Lipoproteins are ordinates mixtures of lipids with proteins
lipoprotein classes
chylomicrons: take lipids from small intestine through lymph cells
very low density lipoproteins (VLDL)
intermediate density lipoproteins (IDL)
low density lipoproteins (LDL)
high density lipoproteins (HDL)
Lipoprotein structure
LDL
molecule
Phospholipids
Cholesterol
Cholesteryl esters
The apolipoproteins
Apolipoprotein Structure:
Amphipathic -helices (polar along one surface of a
helix and hydrophobic along the other side) are
common structural motifs.
One view is that these -helices may float on the
phospholipid surface of the lipoprotein.
Some domains of apolipoproteins have roles in
interaction of lipoproteins with cell surface
receptors.
Chylomicrons,
VLDL, and
their catabolic
remnants
LDL
> 30 nm
2022 nm
HDL
915 nm
Chylomicron
formed through extrusion of resynthesized
triglycerides from the mucosal cells into the
intestinal lacteals
flow through the thoracic ducts into the
suclavian veins
degraded to remnants by the action of
lipoprotein lipase (LpL) which is located on
capillary endothelial cell surface
remnants are taken up by liver parenchymal
cells due to apoE-III and apoE-IV isoform
recognition sites
Chylomicron
Metabolism
Long-chain fatty
acids are reesterified into
triacylglycerols in
the gut and
transferred;
chylomicrons
which contain
apoB48 are
synthesized and
secreted into the
blood via the
lymphatic
circulation
Chylomicron
Metabolism
ApoCs, apoE and
cholesteryl esters are
acquired from HDL in
circulation.
ApoA-I and apoA-IV
may be acquired from
either the intestine or
from HDL in
circulation.
Chylomicron
Metabolism
ApoC-II activates
lipoprotein lipase which
catalyses the hydrolysis
of triacylglycerols
Chylomicron
Metabolism
Apolipoproteins are
transferred back to HDL
Chylomicron
Metabolism
The chylomicron
remnant is taken up by
the apoB48/remnant
receptor in the liver
TG Rich: VLDL
Surface Monolayer
Phospholipids (12%)
Free Cholesterol (14%)
Protein (4%)
Hydrophobic Core
Triglyceride (65%)
Cholesteryl Esters (8%)
VLDL Biogenesis
Microsomal
TG transfer
protein
(MTP)
Facilitates the
translocation,
folding of apoB
and addition of
lipids to lipid
binding domains
TG and
cholesterol are
synthesized in
the liver as
VLDL which
contains apoB100
VLDL
Metabolism
Apo Cs and apoE and
cholesteryl ester are
acquired from HDL in
circulation
ApoC-II activates
lipoprotein lipase which
catalyses the hydrolysis
of TG
VLDL
Metabolism
Apolipoproteins are
transferred back to HDL
VLDL
Remnant
Uptake
The remnant particle (IDL),
if it contains apoE, can be
taken up by the
apoE/remanant receptor
VLDL
Conversion to
LDL
Further action on IDL by
hepatic lipase loses
additional apolipoproteins
(apoE) becomes and is
converted to LDL
CE Rich: LDL
Surface Monolayer
Phospholipids (25%)
Free Cholesterol (15%)
Protein (22%)
Hydrophobic Core
Triglyceride (5%)
Cholesteryl Esters
(35%)
Cholesterol and
Atherosclerosis,
LDL is removed by
apoB100 receptors
which are mainly
expressed in the
liver
LDL Metabolism
Hepatic
Lipase
Cholesteryl
ester transfer
protein
Corneal arcus
Tendon xanthoma
Tendon xanthoma
CE Rich: HDL
Surface Monolayer
Phospholipids (25%)
Free Cholesterol (7%)
Protein (45%)
Hydrophobic Core
Triglyceride (5%)
Cholesteryl Esters
(18%)
Cholesterol and
Atherosclerosis,
HDL Subpopulations
Particle Shape
Apolipoprotein Composition
Discoidal
Spherical
A-I HDL
Particle Size
Lipid Composition
TG, CE, and PL
HDL
Maturation
HDL is secreted in a
discoidal form from the liver
and gut.
As it acquires cholesterol
from tissues in the
circulation, it matures into a
spherical form through the
action of lecithin:cholesterol
acyl transferase
Cholesterol and
Atherosclerosis,
HDL Metabolism
HDL Metabolism
HDL Metabolism
HDL Metabolism
HDL Interconversions
Cholesterol
Homeostasis
Hepatic
Cholesterol
Metabolism
Hepatic
Cholesterol
Metabolism
Only
pathway for
cholesterol
degradation
Rate Limiting
LDL dissociates from the receptor; the receptor recycles to the membrane
Hepatic
Cholesterol
Metabolism
Development of
Athersoclerosis
O cclusive
Thrombus
I ntimal I nu
j ry
Fatty Streak
Lipid- Rich
Plaque
Plaque
Disruption
20
Fibr omuscular
Thrombus
Lysis
Response
O cclusion
40
50
Age (years )
60
Endothelial Dysfunction
Formation of Advanced,
Complicated Lesion
Development of Unstable
Fibrous Plaque
Rupture or ulceration of
fibrous cap rapidly leads to
thrombosis.
Occurs primarily at sites of
thinning of the fibrous cap.
Thinning is a result of
continuing influx of and
activation of macrophages
which release
metalloproteinases and other
proteolytic enzymes.
These enzymes degrade the
matrix which can lead to
hemorrhage and thrombus
formation
Thrombus
Fibrous cap
1 mm
Illustration courtesy of Frederick J. Schoen, M.D., Ph.D.
Lipid core