Professional Documents
Culture Documents
Service Manual
Copyright
2008 Shenzhen Mindray Bio-medical Electronics Co., Ltd. All rights Reserved.
For this Service Manual, the issued Date is 2008-12 (Version: 1.0).
other countries. All other trademarks that appear in this manual are used only for editorial
purposes without the intention of improperly using them. They are the property of their
respective owners.
all installation operations, expansions, changes, modifications and repairs of this product
are conducted by Mindray authorized personnel;
the electrical installation of the relevant room complies with the applicable national and
local requirements;
Be sure to operate the analyzer under the situation specified in this manual;
otherwise, the analyzer will not work normally and the analysis results will
be unreliable, which would damage the analyzer components and cause
personal injury.
II
Warranty
THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES,
EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITY OR
FITNESS FOR ANY PARTICULAR PURPOSE.
Exemptions
Mindray's obligation or liability under this warranty does not include any transportation or other
charges or liability for direct, indirect or consequential damages or delay resulting from the
improper use or application of the product or the use of parts or accessories not approved by
Mindray or repairs by people other than Mindray authorized personnel.
This warranty shall not extend to:
any Mindray product which has been subjected to misuse, negligence or accident;
any Mindray product from which Mindray's original serial number tag or product
identification markings have been altered or removed;
Return Policy
Return Procedure
In the event that it becomes necessary to return this product or part of this product to Mindray,
the following procedure should be followed:
1.
Obtain return authorization: Contact the Mindray Service Department and obtain a
Customer Service Authorization (Mindray) number. The Mindray number must appear on
the outside of the shipping container. Returned shipments will not be accepted if the
Mindray number is not clearly visible. Please provide the model number, serial number,
and a brief description of the reason for return;
2.
Freight policy: The customer is responsible for freight charges when this product is
shipped to Mindray for service (this includes customs charges);
3.
Return address: Please send the part(s) or equipment to the address offered by Customer
Service department.
III
Company Contact
Manufacturer:
Address:
Phone:
Fax:
IV
Table of Contents
1
Introduction............................................................................................................ 1-1
1.2
1.3
1.4
1.5
Introduction............................................................................................................ 2-1
2.2
2.3
2.4
2.5
3.2
3.3
3.4
3.5
Connections......................................................................................................... 3-23
3.6
3.7
3.8
3.9
Table of Contents
4.1.3
4.1.4
4.1.5
4.1.6
4.1.7
4.1.8
4.2
4.3
5.2
5.3
5.4
5.5
5.6
Table of Contents
5.7
5.8
5.9
Maintenance......................................................................................................... 6-1
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
Table of Contents
6.8.3
6.9
6-45
Replacing the sample transport unit....................................................... 6-46
7.2
7.3
Adjustment........................................................................................................... 7-12
7.3.1
Coarse adjustment ................................................................................. 7-12
7.3.2
Preparation before fine tuning................................................................ 7-18
7.3.3
Fine tuning.............................................................................................. 7-19
7.3.4
Gain setup .............................................................................................. 7-24
7.4
8.2
Accessories ...........................................................................................................A-1
B.
C.
D.
E.
Tubing....................................................................................................................E-1
F.
Method to identify cross network cable and direct-connected network cable ....... F-1
1.1 Introduction
The chapter explains how to use the BC-5380 service manual. This manual provides the
reference information and procedures needed in servicing the BC-5380. Before servicing the
BC-5380, read and understand the manual carefully for servicing the equipment properly and
for your safety.
This manual is to be used in conjunction with the operators manual of BC-5380.It does not
contain information and procedures already covered in the operators manual of BC-5380.
1-1
Reagent systems
Quality control
Troubleshooting concepts
The ability to
1-2
See
Chapter
Software Upgrade
Chapter 6 Maintenance
Chapter 8 Troubleshooting
System
Installation
and
of the BC-5380
learn about the main spare parts of the BC-5380
Appendix A Accessories
the BC-5380
Table
Appendix E Tubing
1-3
all capital letters enclosed in [ ] indicate a key name (on the external keyboard), such as
[ENTER].
bold letters included in indicate text you can find on the screen, such as Clean.
bold letters indicate chapter titles, such as Chapter 1 Using This Manual.
All illustrations in this manual are provided as examples only. They may not necessarily reflect
your analyzer setup or data displayed.
1-4
1.5 Symbols
You will find the following symbols in this manual:
When you see
Then
read the statement below the symbol. The statement is
alerting you to an operating hazard that can cause
personnel injury.
read the statement below the symbol. The statement is
alerting you to a possibility of analyzer damage or
unreliable analysis results.
read the statement below the symbol. The statement is
alerting you to information that requires your attention.
read the statement below the symbol. The statement is
alerting you to a potentially biohazardous condition.
You may find the following symbols on the analyzer, reagents, controls or calibrators.
When you see
It means
CAUTION, CONSULT ACCOMPANYING
DOCUMENTS.
BIOLOGICAL RISK
HIGH VOLTAGE
1-5
EARTH (GROUND)
ALTERNATING CURRENT
BATCH CODE
USE BY
SERIAL NUMBER
DATE OF MANUFACTURE
MANUFACTURER
TEMPERATURE LIMITATION
IRRITATING SUBSTANCE
THE FOLLOWING DEFINITION OF THE
WEEE LABEL APPLIES TO EU MEMBER
STATES ONLY: THE USE OF THIS
1-6
1-7
Connect the analyzer to a socket having sole fuse and protective switch. Do
not use the same fuse and protective switch with other equipment (e.g. life
supporting equipment). Otherwise, the equipment failure, over current or
impulse current that occurs at the startup moment may lead to tripping.
To prevent personal injury during the maintenance, keep your clothes, hairs
and hands from the moving parts, such as sample probe, clipper and
piercer.
The reagents are irritating to eyes, skin and diaphragm. Wear proper
personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe
laboratory procedures when handling them in the laboratory.
If the reagents accidentally spill on your skin, wash them off with plenty of
water and if necessary, go see a doctor; if the reagents accidentally spill into
your eyes, wash them off with plenty of water and immediately go see a
doctor.
1-8
The sample probe tip is sharp and may contain biohazardous materials.
Exercise caution to avoid contact with the probe when working around it.
1-9
System Structure
2.1 Introduction
Hardware
Hardware collects signals, controls and drives moving parts, and processes and displays
information. It mainly consists of the mother board, drive board, data board, power board, laser
control board, FS pre-amplification board, SS pre-amplification board, volumetric board,
indicator board, liquid-level detecting board, key board and autoloader control board.
Interface
The system mainly consists of two parts, the main unit (analyzer) and the external computer,
which are connected through the network port. Other connections are realized through the
interfaces of the external computer.
The system software consists of the main unit software and the operation software. The main
unit software is operated on the data board inner the analyzer; the operation software is
operated under the WINDOWS platform of the external computer. The main unit software
analyzes the sequence, collects and identifies data. The operation software displays and prints
the results and stores them into the database, and realizes the interaction of the functions
including count, QC, calibration, maintenance, data management and communication, etc.
Fluidic System
The fluidic system indicates the tubing where reagents, samples and air pass in the analyzer.
The fluidic system controls the correlatively jointed fluidic parts in a set sequence by the
software and driving of the hardware to control the distribution and direction of the medium.
2-1
System Structure
2-2
System Structure
2-3
System Structure
1---Front cover
2---Power/Status indicator
3---[OPEN] key
4---[RUN] key
5---Tube
6---Tube rack
7---Tube
8---Adapter
2-4
System Structure
8 --- AC input
2-5
System Structure
2---Power switch
2-6
System Structure
1---Door lock
2---Tube
3---Tube rack
2-7
System Structure
2 --- Tube
2-8
System Structure
2-9
System Structure
15 ---Mix mechanism
2-10
System Structure
Any screen
Run
STAT
Diluent
Worklist
Graph
Table
QC
Logout
Shutdown
Exit
2-11
System Structure
RUN
AL-WB
Start
Ok
Cancel
CT-WB
Start
Ok
Cancel
CT-PD
Start
Ok
Cancel
Figure 2-9 Menu structure of the Run message box
3. Worklist screen
At the Worklist screen, you can click the function buttons to perform the corresponding
worklist-related operations.
The menu structure of the Worklist screen is shown in Figure 2-10.
2-12
System Structure
Graph
The menu structure of the graph review screen is shown in Figure 2-11.
2-13
System Structure
Table
The menu structure of the table review screen is shown in Figure 2-12.
TABLE
Sample List
Samples
within Today
Delete
Deselect
Print
All Samples
Trend Graph
Samples
Found
Optical
Result
Edit Result
Restore Result
Store
Sample/
Patient Info.
Validate
Batch Validate
Search
Microscopic
Exam. and
Others
Transmit
Pos./Total
Others
Data Backup
Data Export
Auto-backup
Auto-restore
Data Export
The menu structure of the Data Export screen is shown in Figure 2-13.
2-14
CV
System Structure
Data Backup
The menu structure of the Data Backup screen is shown in Figure 2-14.
Compare
2-15
System Structure
COMPARE
Comparison
Summary
Re-fill
Search
Adjust Parameter
Order
Print
Result Trend
Re-fill
Search
Adjust Parameter
Order
Print
L-J
2-16
System Structure
2-17
System Structure
X-B
Settings
Set Limits
Have Preset
Values
Restore
Defaults
Save
Print
Graph
Delete
Calculate
Preset Values
Save Preset
Values
Print
Pos./Total
List
Delete
Print
Communication
Data Backup
Data Export
History
Pos./Total
2-18
System Structure
2-19
System Structure
X -R
X -R
Settings
Save
Print
Run
Start
Print
Pos./Total
Graph
Delete
New Vial
Data Compare
Display order
Outliers
Print
Pos./Total
Table
Delete
Print
Communication
Data Backup
Data Export
History
Pos./Total
6. Service screen
The Service screen contains screens of Maintenance, Status, Self-test, Debug,
Log, Counter and Version and Config. Information. The menu structures of these
2-20
System Structure
screens are shown below.
Maintenance screen
2-21
System Structure
2-22
System Structure
Status screen
Status
Temperature&Pressure
Export
Print
Voltage&Current
Export
Print
Sensor
Export
Print
Signal Collection
Export
Print
Self-test screen
2-23
System Structure
2-24
System Structure
Debug screen
Counter screen
Log screen
2-25
System Structure
The menu structure of the Version and Config. Information screen is shown in Figure 2-26.
Figure 2-26 Menu structure of the Version and Config. Information screen
2-26
System Structure
7. Setup screen
The Setup screen contains screens of Gain, Data Format, Reagent, Auxiliary, Para.
Unit, Ref. Range, Print, Auto Maintenance, User and Password and Advanced.
The menu structure of the Setup screen is shown in Figure 2-27.
2-27
System Structure
SETUP
Date Format
Reagent
Auxiliary
Para. Unit
Apply
Ref. Range
Print
Ok
Communication
RUO
Cancel
Gain
Auto Maintenance
Autoloader
Barcode Info.
Lab Info.
Ok
Cancel
Advanced
Maintenance
User and
Password
New
Edit
Delete
Reset Password
Change Password
Close
8. Statistics screen
2-28
System Structure
The menu structure of the Statistics screen is shown in Figure 2-28.
Calculate
Workload
STATISTICS
Statistical
Condition
Re-fill
Statistical
Item
Statistics
Workload
Summary
Adjust Order
Print
Calculate
Charge
Statistical
Condition
Re-fill
Statistics
Charge
Summary
Adjust Order
Print
Figure 2-28 Menu structure of the Statistics screen
2-29
System Structure
Contents
Service
Review
Search
Daily
Operations
Print
Setup
Calibration
QC
Figure 2-31 Menu structure of the Help screen
12. Shutdown
The menu structure of Shutdown is shown in Figure 2-32.
2-30
System Structure
SHUTDOWN
Sleep
Shutdown
13. Exit
The menu structure of Exit is shown in Figure 2-33.
2.5.2 Passwords
The passwords are divided into three levels: common user, administrator (set by the user at
the User and Password screen) and service engineer (user name: service, and password:
Se s700. Note: there is a space between e and s). An administrator is enabled to have all
the authorities of a common user, and a service engineer is enabled to have all the authorities
of an administrator. Tables below introduce the authorities enabled for each level of user.
Module
Worklist
Screen
Authorities enabled
Worklist
User
New,
Save,
Search,
Insert,
Hide,
2-31
System Structure
Count
All
screens
except the QC
Run
and
Service
(Maintenance,
Status,
Self-test
and
Debug);
as
well
as
the
Run screen
Review
Graph
Data/Graph,
Saving
of
DIFF,
Print,
and
Restore
Result
are
Data Export
Data Backup
Data Backup
Compare
and
parameters
in
the
Auto-backup
Auto-restore
Auto-restore
2-32
System Structure
QC
L-J
Data
Export,
History
and
Pos./Total
X-B
Data
Export,
History
and
Pos./Total
X QC setup: browse file information of
Pos./Total
X QC graph: New Vial, Data Compare,
Backup,
Pos./Total
2-33
Data
Export,
History
and
System Structure
X -R
Pos./Total
X -R QC graph: New Vial, Data Compare,
Display
Order,
Outliers,
and
Pos./Total
X -R QC table: Print, Communication,
Maintenance
Replace
Reagent
(All
Reagents
Temperature&Pressure
Analyzer
box),
Voltage&Current
Self-test
for
syringes
and
sampling
Log
Version
Setup
and
Config.
Information
screen
General
Date
Format
(Authority unavailable)
Format,
Reagent,
Auxiliary
and
Password
2-34
System Structure
Statistics
Lab
Information
to edit
Select
Calculate
Calibration
Shutdown
Exit
set
statistical
summaries
Calculate
Charge
charge summaries
Department
Check
Deliverer
Check
Diagnosis
Check
Gender
Check
Calibration
Factor
Help
items,
Workload
Shortcut Code
statistical
Help
Shutdown
the
Sleep
and
Shutdown
procedures
Exit
Module
Screen
Worklist
Worklist
Run
All
Authorities enabled
screens
except the QC
Run
Service
(Maintenance,
Status,
Self-test
and
Debug)
and
Calibration
Review
Graph
Table
Data Export
2-35
System Structure
Data Backup
Compare
Auto-backup
Auto-restore
QC
L-J
X-B
X -R QC count
X -R QC graph: Delete
X -R QC table: Delete
Service
Maintenance
Status
Temperature&Pressure
(Analyzer
Information
available
at
the
message
box),
Voltage&Current
Version
and
Config.
Information
2-36
System Structure
Authority setting items of the Auxiliary
Setup
screen
General Setup
User
and
Password
Lab
Information
Shortcut Code
Statistics
Calculate
Workload
Calculate
Charge
Shortcut Code
Calibration
Department
Deliverer
Diagnosis
Gender
Calibration
Factors
Calibrator
Fresh Blood
Help
Help
Shutdown
Shutdown
Exit
Exit
Module
Worklist
Screen
Authorities enabled
Worklist
2-37
System Structure
Engineer
Run
All
screens
except the QC
Run
Service
(Maintenance,
Status,
Self-test
and
Debug)
and
Calibration
Review
Graph
Special Info.
Table
Data Export
Data Backup
Compare
Auto-backup
Auto-restore
QC
L-J
X-B
X
X -R
Service
Maintenance
Status
Temperature&Pressure
(Analyzer
box),
Voltage&Current
and
Export
the
Analyzer
Information
Self-test
Counter
has
been
calculated
except
2-38
System Structure
Log
Debug
Version
Debug
and
Config.
Information
Setup
User
and
Password
Lab
Information
Shortcut Code
Advanced
Statistics
Maintain
Calculate
Workload
Calculate
Charge
Shortcut Code
Department
Deliverer
Diagnosis
Gender
Calibration
Calibration
Factors
Calibrator
Help
Help
2-39
System Structure
Shutdown
Shutdown
Exit
Exit
2-40
3.1 Preparations
3.1.1 Purpose
Install the instrument and update the software properly as per the procedures introduced in this
chapter.
3.1.2 Tools
Blade or clipper
Pipette (200l)
Cross-headed screwdriver
The software copied in the U-drive must be the specified one for the
analyzer of the specified model. Otherwise, you can not proceed to install.
3.1.3 Accessories
Glass Pipette
7m standard particles
Gloves
Tissues
3-1
at least 50 cm behind;
enough room on or below the countertop to accommodate the diluent, rinse and waste
containers
Please place the analyzer near the electrical outlet to avoid using the
plug-board.
3. Please use the original electrical wire shipped with the analyzer. Using other electrical wire
may damage the analyzer or cause unreliable analysis results.
Voltage: A.C. 100V-240V
Input power: 300 VA
Frequency: 50/60 Hz
3-2
3-3
Operating
system
RAM
3-4
Screen resolution
Disk space
3-5
3-6
3-8
When lifting the main unit, keep it as level as possible and avoid strong
impact.
3-9
Figure 3-11 Remove the plastic cable tie and the clamp
2. When using the tubes of 1475mmor 1575mmmodel, you should disassembly
the adapter from the rack to load the tubes. Disassembly as shown in Figure 3-12.
3-11
3-12
3-13
3-14
3-15
3-16
If it is found that the main unit software mismatches the PC software, you
should upgrade the main unit software as per the procedures specified in
3.11.2 to match it with the software of the PC-end.
16. Install the driver program of the printer properly on the PC.
17. The BC-5380 consists of the IPU PC and the main unit as designed. Only basic interactive
keys like the [RUN] and [OPEN] keys are located on the main unit. The rest of the
operations are realized through the IPU PC. The IPU PC has two network interfaces, one
connecting the BC-5380 and the other one may connect to other information devices (e.g.
LIS system) or the LAN of the hospital, which are used to export data. There are two icons
of Local Area Connection for the two network interfaces existed. follow the procedures to
configure the network interfaces of the IPU PC:
(1) Right click My Network Places and select Properties from the pop-up dialog box.
3-17
3-18
Never set the Default gateway and DNS server for the network interface
(10.0.0.1) connecting the BC-5380. Otherwise, you may not access to the
network connecting the other network interface.
18. The LIS computer is used to transmit data by connecting the IPU computer. Do as follows
to check and set the IP for the LIS computer.
(1) At the desktop of the LIS computer, right click My Network Places and select
Properties from the pop-up dialog box, as shown in Figure 3-28 .
3-19
3-20
3-21
3-22
3.5 Connections
1. See Figure 3-32 for connections of reagent and waste containers. Note that the
M-53LEO(I) lyse, M-53LEO(II) lyse, M-53LH lyse and the analyzer should be placed in a
plane of the same level, and the M-53D diluent, M-53 cleanser, and waste containers
should be placed under the countertop.
Make sure the M-53LEO (I) lyse, M-53LEO (II) lyse, M-53LH lyse and M-53
cleanser are placed on the same level as the analyzer.
3-23
2. Locate the power inlet at the back of the main unit and connect the female end of the
power cable to the power inlet, and connect the three-pronged end of the power cable to a
power outlet.
3. Connect the BC-5380 with the IPU computer by a cross network cable, as shown in Figure
3-35 .
3-24
Figure 3-35 Connecting the main unit and the IPU computer
Be sure to use the cross network cable to connect the IPU computer and the
LIS computer.
The two ends of a cross network cable have no difference. You can plug
them to IPU computer and the LIS computer respectively at will. Please see
appendix H for method of how to distinguish a cross network cable from a
direct-connected network cable.
4. There are two ways to connect the BC-5380 and the LIS computer.
(1) Connect the BC-5380 with the LIS computer by a cross network cable, as shown in Figure
3-36 .
Figure 3-36 Connecting the IPU computer and the LIS computer
Be sure to use the cross network cable to connect the analyzer and the LIS
computer.
The two ends of a cross network cable can be randomly plugged into the
two computers respectively.
(2) Connect the IPU computer to the LAN of the hospital, as shown in Figure 3-37 .
3-25
Adopt one of the two ways to finish the network connection according to the condition of the
hospital or doctors requirement.
5. Connect the printer with the IPU computer with specified cable and connect the power
cord properly.
3-26
3.6 Start-up
3.6.1 Inspection before Startup
Check and make sure the marks on the relieve valve are on the same line, as shown in Figure
3-38. Change the relieve valve if the marks are not on the same line.
3-27
3-28
Two-way
connector
Tube 1
Tee connector
Figure 3-42 Checking the pinch valve
will appear
on the task bar. It means the local-connection-related network interface connects the
appears on the
task bar, it means the local-connection-2-related network interface doesnt connect the
BC-5380, and it is available to connect the LAN of the hospital. If the two prompts are all
3-29
3-30
The IP address you set here should be the one of the LIS computer
connecting the IPU computer. The Port you set here should be the
interception port of the LIS computer. The IP address of external server
cannot be 10.0.0.1 and 10.0.0.2, and format like 10.0.0.* is not recommended.
5. Before transmitting data, make sure the LIS computer connecting the IPU computer is
working. Thus, the IPU program could locate the destination for transmitting.
6. If the connection is established between the IPU computer and the LIS computer, the LIS
icon in the IPU screen will turn to colorful, as shown in Figure 3-46, indicating you can start
transmitting data now.
3-31
The IPU computer can connect LIS computer through cross network cable;
connect LIS computer in the same network through devices like HUBs or
exchangers; connect LIS computer in a different network through devices
like routers.
3-32
3-33
The specified version here means the PC end software should be complied
with that of the main unit end. Upgrade the main unit end software to match
the PC end software if necessary.
3-34
3-35
3-36
3-37
Be sure to adjust the position of the volumetric tube by your hand directly.
Never pull the tee connector hard to adjust, otherwise, the tee connector
may leak and the volumetric tube may be broken.
3-40
Select printer
Click OK
3-41
HGB gain
HGB blank
voltage
SS 0.1max total
FS 0.1max total
Click Calculate
Click OK
3-44
Be sure to use the distilled water to prepare the standard particle sample.
Prepared standard particle sample should be run within 12 hours.
8. When finish running, enter the Table screen and select the standard particle sample just
run, and then click the Optical button, as shown in Figure 3-65.
3-45
3-46
Background Count
Back to the Graph screen and perform the background count in WB-CBC+DIFF mode. The
background results shall meet the specified requirement: WBC:0.1109/L, RBC:0.031012/L,
HGB:1g/L, HCT:0.5%, PLT:5109/L.
3.10.2
Carryover
Follow the procedure 3 instructed in section 9.3.1 of the Operators Manual to make sure the
carryover of the analyzer meets the requirements listed in the table below:
3.10.3
Parameter
Carryover
WBC
0.5
RBC
0.5
HGB
0.6
HCT
0.5
PLT
1.0
Reproducibility
Condition
WBC
(6.015.0)109/L
2.0%
12
RBC
(4.006.00)10 /L
1.5%
HGB
(110180)g/L
1.5%
MCV
(80110)fL
1.0%
PLT
(150500)10 /L
4.0%
Condition
WBC
(6.015.0)10 /L
4.0%
RBC
(4.006.00)1012/L
3.0%
HGB
(110180)g/L
3.0%
MCV
(80110)fL
2.0%
PLT
(150500)109/L
8.0%
3-47
3.10.4
Calibration
1. Click MenuCalibration, and then click Calibrator to enter the screen. Select the
calibration mode as Whole Blood; enter the lot No. and the expiration date of the
calibrator, and then enter the WBC, RBC, HGB, MCV and PLT targets of the calibrator into
the table.
3-48
Be sure to immerse the tip of the sample probe into the calibrator (1 ml at
least).
3. Repeat running the calibrator for 11 times as instructed by Step 2.Then, click the Start
button. The setup of whole blood calibration factors is completed. Click Diluent and a
message box will pop up. Put the tube into the tube holder and put the loaded tube holder
into the sample compartment, and then close the compartment door manually. Then press
the [RUN] key to start dispensing the diluent, as Figure 3-68 shows. Dispense the diluent
into the tube for 6 times consecutively. Then click the Cancel button to close the
message box. Press the [OPEN] key to open the sample compartment and take out the
tube and the tube holder.
3-49
Click
Diluent
Then click
Cancel
3-50
Be sure to immerse the tip of the sample probe into the calibrator (at least
1ml).
8. Running the calibrator consecutively for 11 times as instructed by Step 8. The setup of
predilute calibration factors is completed.
3.10.5
Linearity
3-52
We only update the IPU software provided that it has been completely installed on the PC. The
update will not change users custom configuration including settings and database. Follow the
following steps to update the software:
4. When the program finishes the installation, a message box shown in Figure 3-74 will pop
up;
3-53
5. Click the Next button to update the database, as shown in Figure 3-75;
6. When all the database files are installed, a message box shown in Figure 3-76 will prompt
3-54
To update the Version 1.3 BC-5100 software, select the Install mode to
install the new version in the previous folder rather than update the outdated
software.
If the system prompts for not enough space on disk during installation,
select another disk to install and make sure the free space on that disk is at
least 4G.
3.11.2
Before perform this step, make sure that the analyzer is powered on for at
least 30 seconds. Otherwise, the connection to the main unit may fail after
reboot or the update server may be uploaded again after being connected to
the main unit.
3-55
3-56
3-57
At this time, the update program has already been disconnect from the
analyzer, so Step 1 and 2 should be performed again to launch the update
program.
After the update server program being successfully updated and main unit
being rebooted, wait 2 minutes before logging on to the client.
5. After logging on, a message box shown in Figure 3-83 will pop up, asking you to choose
the directory of the update files;
3-58
3-59
9. After finishing uploading, a message box of successful uploading shown in Figure 3-87 will
pop up;
10. Click Ok and wait for a while. A message box will pop up to show the items to be updated,
as shown in Figure 3-88;
3-60
3-61
Use the dedicated cord in the accessory box provided by the manufacturer.
15. Update the folder step4 (including the hardware writing software) according to the same
process, and then switch off the analyzer.
Do not delete the files under ftp://10.0.0.2/3101 before updating and keep the
power supply of the main unit on. Always update the hardware after Step2 is
updated successfully.
After
replacing
the
data
board
(including
rootfs,
material
code:
3-62
Fluidic System
4.1.3 Pumps
P1: provides pressure for the pressure chamber.
P2: empties the DIFF bath and vacuum chamber and creates vacuum.
P3: empties the probe wipe, WBC bath and RBC bath.
4.1.4 Syringes
Aspiration-Syringe: full volume 100 l; it aspirates and dispenses samples and aspirates
the second diluted sample.
Diluent-Syringe: full volume 10ml; it dispenses diluent into the WBC and RBC bath and
supplies the diluent to the probe wipe.
Lyse-Syringe: full volume 2.5ml; 3 syringes are driven by a motor; it dispenses M-53Leo
(I), M-53Leo (II) and M-53H.
Sheath-Syringe: full volume 10ml; it dispenses the sheath into the flow cell, cleans the
flow cell and DIFF bath, and is also be used for sample preparation.
Sample-Syringe: full volume 250l; it dispenses the sample into the flow cell.
4.1.5 Valves
Fluidic valve: turns on and off by electromagnetic force; controls the fluidic or air flow
direction.
Pinch valve: turns on and off by electromagnetic force; starts/stops the fluidic flow.
4.1.6 Baths
WBC bath: consists of a front bath, back bath and aperture. The WBC sample mixes and
reacts here and it is used for the measurement of HGB and WBC.
RBC bath: consists of a front bath, back bath and aperture. The RBC sample mixes and
4-1
Fluidic System
reacts here and it is used for the measurement of RBC/PLT.
Vacuum chamber: creates and stores stable vacuum for counting the WBC and RBC by
impedance method; cleans the back bath and empties the volumetric tube.
Pressure chamber: creates and stores stable pressure to generate bubbles for the baths.
WBC volumetric tube: volume 500ul; it measures the volume of the WBC sample
RBC volumetric tube: volume 300ul; it measures the volume of the RBC sample
4.1.8 Filters
Filter of WBC volumetric tube: it filters the air entered the WBC volumetric tube
Filter of RBC volumetric tube: it filters the air entered the RBC volumetric tube
4-2
Fluidic System
2 analysis modes: Open vial-whole blood mode and open vial-predilute mode.
Table 4-1 Reagent volume required when running samples
Reagents
Name
M-50 Diluent
M-53 Lyse
CBC
M-53D Diluent
45mL
34mL
M-53LEO(I) Lyse
1.1ml
M-53LEO(II) Lyse
0.135ml
M-53LH Lyse
0.5ml
Name
M-50 Diluent
M-53D Diluent
89ml
M-53LEO(I) Lyse
M-53LEO(II) Lyse
M-53LH Lyse
2ml
M-53 Cleanser
M-53 Lyse
Cleanser
Name
M-50 Diluent
M-53D Diluent
172ml
M-53LEO(I) Lyse
M-53LEO(II) Lyse
M-53LH Lyse
2ml
M-53 Cleanser
M-53 Lyse
Cleanser
Starting up a drained system (after the Prepare to Ship procedure has be done)(excluding
startup background)
Fluidic System
Table 4-4 reagents volume required for starting up a drained system (after the Prepare to
Ship procedure has been done)
Volume of starting up a
Reagents
Name
M-50 Diluent
M-53 Lyse
Cleanser
M-53D Diluent
207ml
M-53LEO(I) Lyse
12ml
M-53LEO(II) Lyse
12ml
M-53LH Lyse
12ml
M-53 Cleanser
8ml
Name
M-50 Diluent
M-53D Diluent
53ml
M-53LEO(I) Lyse
M-53LEO(II) Lyse
M-53LH Lyse
M-53 Cleanser
29ml
M-53 Lyse
Cleanser
4-4
Fluidic System
4-5
Fluidic System
M-53LH Lyse: it lyses erythrocytes and platelets and separates basophiles from other WBCs.
Diluent: it is used for cleaning and provides the environment for reaction and measurement.
Function description: 6l blood sample, 2.5ml diluent and 0.5ml LH Lyse are mixed in the
bath, and then they react. After reaction, the sample flows to the back bath via the
aperture, driven by the vacuum, and the blood cells are counted when passing through
the aperture. The volume of the sample is measured by the volumetric tube.
DIFF channel
LEO (I) and LEO (II): they lyse RBCs and differentiate WBCs into 4 sub-populations.
Diluent: cleans the fluidic system and provides sheath.
Function description: 0.6ml Leo (I) is dispensed into the bath as the initial volume, and
then 9l blood sample and 0.6ml Leo (I) are dispensed. After the sample is well mixed and
reacts with the reagent for a while, 0.135ml Leo (II) is dispensed. After the sample reacts
with the reagents for a while, the sample is driven to the bottom of the flow cell and the
sheath flow is enabled. Then the sample is dispensed into the flow cell by the sample
syringe for measurement.
4-6
Fluidic System
RBC/PLT channel
Diluent: dilutes samples, washes, provides a conductive environment and processes the cells
Function description: 52.08l sample dilution (1:417.7) is aspirated by the sample probe
from the WBC bath. The sample probe then moves to the RBC bath and mixes the
sample with 2.5ml diluent to dilute the sample to the ratio of 1:20000. Then, the mixed
sample is driven into the back bath through the aperture by the vacuum, and the blood
cells are counted when passing through the aperture. The volume of the sample is
measured by the volumetric tube.
Principles: Colorimetry
Parameters: HGB
The HGB unit calculates the concentration of HGB by comparing the light passing through
the sample with the blank reference reading.
4-7
Fluidic System
4.3 Sequence
of
Whole
Blood
CBC+DIFF
Measurement
4.3.1 Sampling and dispensing procedures
A.
20l sample is aspirated by the aspiration syringe from the sample tube, and then the
sample probe ascends while its exterior is being cleaned.
The sample probe moves to above the DIFF bath.
The sample probe descends into the DIFF bath and dispenses 9l sample.
B.
First Diluting
The sample probe ascends from the DIFF bath, meanwhile the probe exterior is cleaned.
The sample probe moves to above the WBC bath
The sample probe descends into the WBC bath, and then dispenses 6l sample. The
dispensed sample is diluted by the 2.5ml diluent that is already there to form the 1:417.7
dilution.
C.
The sample probe ascends from the WBC bath, meanwhile the probe exterior is cleaned.
The sample probe dispenses diluent into the probe wipe from the top to wash off the residual
sample.
The sample probe descends again into the WBC bath and aspirates the diluted sample.
D.
The sample probe ascends from the WBC bath, meanwhile the probe exterior is cleaned.
The sample probe moves above the RBC bath.
The sample probe descends into the RBC bath, and then dispenses 1ml diluted sample. The
dispensed sample is diluted by the diluent that is already in the bath to form the 1:20000
dilution.
E.
The sample probe ascends from the RBC bath, meanwhile the probe exterior is cleaned.
The sample probe returns to the home position.
4-8
Fluidic System
E
Figure 4-3 Dispensing procedures of sample probe
4-9
Fluidic System
The sheath flow of the DIFF channel and the measurement (19.8-40.5s)
During this period:
1. Sheath fluid forming: V23 and V24 are energized. The sheath syringe dispenses the
diluent into the flow cell at a certain speed to form the sheath.
2. Dispensing the sample at a high speed: The sample syringe dispenses the sample into the
flow cell at a high speed.
3. Measurement: when the sample fluid is stable, the sample syringe dispenses the sample
4-10
Fluidic System
into the flow cell at a low speed while the sheath syringe dispenses the diluent into the flow
cell to surround the sample. Thus, the sheath flow passes through the flow cell at a stable
speed for measurement.
The fluidic system status of this period is shown in Figure 4-5.
4-11
Fluidic System
Dispensing 1.5ml diluent for soaking: valve V22, V23 and V24 are energized. The
sheath syringe dispenses 1.5ml diluent to soak the DIFF bath.
4-12
Fluidic System
Dispensing the blood sample: the aspiration syringe dispenses 6 l sample into the
WBC bath while mixing the sample by bubbling.
4-13
Fluidic System
4-14
Fluidic System
Fi
gure 4-8 Fluidic status when cleaning the WBC back bath
4-15
Fluidic System
Fluidic System
-300 to -260 HP, the sample in the front bath flows to the back bath in the direction
shown in the following figure. The sample volume measured is determined by the diluent
volume that flows between the two photocouplers of the WBC volumetric tube.
Figure 4-11 Fluidic status when cleaning the back bath and draining the front bath
2. Draining the front bath and cleaning: Valve V35 is energized and waste pump pumps the
remaining sample out of the RBC bath. The diluent syringe dispenses 3ml diluent into the
RBC bath for soaking.
4-17
Fluidic System
3. Cleaning the back bath: The vacuum of -400 to -300HP is created in the vacuum chamber.
Valve V30 and V32 are energized. The volumetric tube is cleaned and bubbles are
discharged from the top of the volumetric tube. Vale V32, V14, V34 and V30 are energized
to clean the back bath, and then the waste is discharged.
A.
15l sample is aspirated by the aspiration syringe, and then the sample probe ascends
meanwhile its exterior is cleaned and the sample probe dispenses 3l sample into the probe
wipe.
The sample probe moves to above the WBC bath
The sample probe descends into the WBC bath and dispenses 6l sample into the WBC
bath.
B.
The sample probe ascends from the WBC bath, meanwhile its exterior is cleaned.
The sample probe dispenses cleanser into the probe wipe from the top to wash off the
residual sample..
The sample probe descends again into the WBC bath and aspirates the diluted sample.
C.
The sample probe ascends from the WBC bath meanwhile its exterior is cleaned.
The sample probe moves to above the RBC bath.
The sample probe descends into the RBC bath, and then dispenses 1ml diluted sample into
RBC bath, where the sample is further diluted by the diluent that is already there to form the
second dilution at the ratio of 1:20000.
D.
The sample probe ascends from the RBC bath meanwhile its exterior is cleaned.
4-18
Fluidic System
The sample probe returns to the home position.
4-19
Hardware System
The BC-5380 hardware system consists of the boards, power execution parts, sensors,
switches and cables. Their relationship is shown below.
Sample
drive
motors
Syringe
drive
motors
Heater
for optics
system
And
Temp.
sensors
Heater
for Diff
pool and
temp.
sensors
Ambie
nce
temp.
sensor
BC-5380
Hardware System
NetPort
Fan
Sensor on right door
Vavles and
pumps
Volume mesure
board and press
sensors
Laser
board
Liquid level
sensor
SS
Pre-amp
board
Mother
Board
Auto-sample
board
FS
Pre-amp
board
Optics couple
sensor
Door driver
Key
Board
WBC Pole
Indication
Board
AC Power
Switch
Data Board
RBC Pole
Power
Supply
Board
Drive Board
HGB Module
Note:
In the figure, the thin arrows represent the cable connections; the thick arrows (connecting the
data board and the driver board) represent the plugboard connections; the square boxes
5-1
Hardware System
represent the boards and the ellipses represent the components.
The boards of the BC-5380 and their functions are shown in the table below.
Table 5-1 Boards of the BC-5380 and their functions
No.
1
Name
Data board
Functions
CPU basic system + FPGA + A/D + Analog
channel
Drive board
Mother board
FS
pre-amplification
board
6
7
SS
scattered light
pre-amplification
board
scattered light
Volumetric board
Indicator board
Power board
10
Liquid-level board
11
Autoloader board
Sample
transport,
mix
Key board
5-2
control,
Hardware System
Function
Power interface
Connectors for board signals (autoloader board, laser control board, pre-amp board,
volumetric board, liquid level checker, key board and indicator board, etc.)
Connectors for component (valves, pumps, motors, heaters, fan, HGB module,
independent key, optics, etc.) signals
Block diagram
5-3
Hardware System
DB9(rs23
2)
RJ45
rs232 int.
driver
Isolate
transfoemer
Power filter
& indicator
Sockets
for
Power
Supply
RS422 int
driver
Sockets for
vavles,pumps,motors,heat
ers
Sockets for
optics sensor
Socket
for
power( Autosa
mple board)
Socket for
Temp. sensor
Socket for
Liq.level
checker
Interface definition
List of connectors
Table 5-2 List of connectors
SN.
PIN Number
Function Description
PA1
96
PA2
96
PB1
96
PB2
96
5-4
Hardware System
signals
PB3
96
J1
J2
J3
10
J4
J5
J6
J7
J8
J9
J10
J11
J12
J13
AC120V connector
J14
J15
12
J16
34
J17
No in use
J18
24
J19
24
J20
24
J21
J22
J23
J24
J25
J26
No in use
J27
J28
J29
20
J30
20
J31
20
J32
20
J33
20
J34
Fan connector
J35
10
Hardware System
J36
J37
D+5V connector
Model: 5535043-4
Table 5-3 Definition of PA1 for mother board
Column A
Column B
Column C
D+5V
D+5V
D+5V
D+5V
D+5V
D+5V
D+5V
D+5V
D+5V
D+5V
D+5V
D+5V
D+5V
D+5V
D+5V
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
10
DGND
DGND
DGND
11
DGND
DGND
DGND
12
TP_TX+
PWFBOUT
TP_RX+
13
TP_TX-
DGND
TP_RX-
14
DGND
DGND
DGND
15
DGND
DGND
DGND
16
TMS_TO_DRV
TMS_TO_AS
UART3_TO_PC
17
TCK_TO_DRV
TCK_TO_AS
UART3_TO_DAT
18
DATA_TO_DRV
DATA_TO_AS
DGND
19
DATA_FROM_DRV
DATA_FROM_AS
DGND
20
DGND
UART1_TO_DAT+
UART1_TO_AS+
21
DGND
UART1_TO_DAT-
UART1_TO_AS-
22
UART0_TO_DRV
DGND
DGND
23
UART0_TO_DAT
DGND
DGND
24
DGND
DGND
#SUCK_KEY
25
DGND
#VM_ON
DGND
26
DGND
#RBC_START
#BUZ
27
DGND
#RBC_STOP
#COUNT_KEY
28
DGND
#WBC_START
FAULT
29
DGND
#WBC_STOP
READY
5-6
Hardware System
30
DGND
DGND
OPTI_DOOR
31
DGND
#LASER_ON
#RIGHT_DOOR
32
DGND
DGND
#INSERT_DOOR
Column B
Column C
AGND
AGND
AGND
AGND
LASER_CURT
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
AGND
10
AGND
AGND
AGND
11
AGND
NC
AGND
12
AGND
AGND
A GND
13
AGND
SS
AGND
14
AGND
AGND
AGND
15
AGND
FS
AGND
16
AGND
AGND
AGND
17
AGND
HGB_IN
AGND
18
AGND
AGND
AGND
19
AGND
HGB+
AGND
20
AGND
HGB-
AGND
21
AGND
A-12V
A-12V
22
AGND
A-12V
A-12V
23
AGND
A+12V
A+12V
24
AGND
A+12V
A+12V
25
AGND
A+12V
A+12V
26
NC
NC
NC
27
NC
A+70V
NC
28
NC
NC
NC
29
NC
NC
NC
30
NC
AC120_B
NC
31
NC
NC
NC
32
AC120_A
NC
NC
5-7
Hardware System
Description
ET_TP+
Transmit, positive
ET_TP-
Transmit, negative
ET_RP+
Receive, positive
ET-NGP
ET-NGP
ET_RP-
Receive, negative
ET-NGR
ET-NGR
Definition
Description
NC
RXD/TXD
Send/receive
TXD/RXD
Receive/send
NC
GND
6-9
NC
Definition
Description
GND
NC
TXD/RXD
Send/receive
RXD/TXD
Receive/send
5-9
NC
5-8
Hardware System
Table 5-8 Definition of J3 for mother board
Pin
Definition
Description
D3V3
GND
GND
TCK
JTAG clock
TMS
DATA_FROM_AS
DATA_TO_AS
NC
GND
10
NC
Definition
#SUCK_KEY
GND
#RIGHT_DOOR
GND
OPTI_DOOR
GND
Description
NC
Right door switch on/off
optical shielding box switch
Definition
UART2_TO_AS+
Description
5474 serial port 2 to positive end of
sample board data
UART2_TO_AS-
UART2_TO_DAT+
UART2_TO_DAT-
Hardware System
TOP PHSERIES
Definition
Description
D+5V
#COUNT_KEY
#INSERT_DOOR
FAULT
READY
NC
#BUZ
GND
Buzzer driven
Definition
NC
AGND
LASER_CURT
A+12V
#LASER_ON
D+5V
Description
5-10
Hardware System
Table 5-13 Definition of J9, J10 for mother board
Pin
Definition
AGND
FS/SS
AGND
AGND
A-12V
AGND
A+12V
MHOLE
Description
FS/SS signal input, analog signal
Definition
HGB+
Description
HGB
light
drive,
light
drive,
positive
2
HGB-
HGB
negative
HGB_IN
AGND
AGND
HGB feedback
Definition
AGND
NC
NC
NC
A+12V
AGND
AGND
A-12V
Description
Hardware System
Connector type: HEADER WTB 2.5MM DIP1*3 TOP XHSERIES
Model: B-3B-XH-A 'JST' 2.5MM
Table 5-16 Definition of J13 for mother board
Pin
Definition
AC120V_A
NC
AC120V_B
Description
A end of 120V AC
B end of 120V AC
column B
column C
HT1_SA
HT1_SB
AMBI_SA
HT2_SA
HT2_SB
AMBI_SB
POSI_PRESS
NEGA_PRESS
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
DGND
10
DGND
DGND
DGND
11
DGND
DGND
DGND
12
D+5V
D+5V
D+5V
13
D+5V
D+5V
D+5V
14
D+5V
D+5V
D+5V
15
D+5V
D+5V
D+5V
16
D+5V
D+5V
D+5V
17
DGND
DGND
DGND
18
#LIQ1
#LIQ2
TMS_TO_DRV
19
#LIQ3
#LIQ4
TCK_TO_DRV
20
#LIQ5
#LIQ6
DATA_TO_DRV
21
#LYSE_SENSOR
#WASTE_SENSOR
DATA_FROM_DRV
22
#LS_ON
DGND
DGND
5-12
Hardware System
23
DGND
DGND
DGND
24
OP17_CTRL
OP17_STS
UART0_TO_DRV
25
OP18_CTRL
OP18_STS
UART0_TO_DAT
26
OP19_CTRL
OP19_STS
DGND
27
OP20_CTRL
OP20_STS
DGND
28
OP1_CTRL
OP1_STS
OP2_CTRL
29
OP2_STS
OP3_CTRL
OP3_STS
30
OP4_CTRL
OP4_STS
OP5_CTRL
31
OP5_STS
OP6_CTRL
OP6_STS
32
OP7_CTRL
OP7_STS
OP8_CTRL
column B
column C
OP8_STS
OP9_CTRL
OP9_STS
OP10_CTRL
OP10_STS
OP11_CTRL
OP11_STS
OP12_CTRL
OP12_STS
OP13_CTRL
OP13_STS
OP14_CTRL
OP14_STS
OP15_CTRL
OP15_STS
OP16_CTRL
OP16_STS
DM_ON
DGND
DGND
DGND
NC
NC
NC
P+24V
SM1_AP
SM1_AP
10
P+24V
SM1_AN
SM1_AN
11
P+24V
SM1_BP
SM1_BP
12
P+24V
SM1_BN
SM1_BN
13
P+24V
SM2_AP
SM2_AP
14
P+24V
SM2_AN
SM2_AN
15
P+24V
SM2_BP
SM2_BP
16
P+24V
SM2_BN
SM2_BN
17
P+24V
SM3_AP
SM3_AP
18
P+24V
SM3_AN
SM3_AN
19
P+24V
SM3_BP
SM3_BP
20
P+24V
SM3_BN
SM3_BN
21
P+24V
SM4_AP
SM4_AP
22
P+24V
SM4_AN
SM4_AN
23
P+24V
SM4_BP
SM4_BP
24
P+24V
SM4_BN
SM4_BN
25
PGND
SM5_AP
SM5_AP
5-13
Hardware System
26
PGND
SM5_AN
SM5_AN
27
PGND
SM5_BP
SM5_BP
28
PGND
SM5_BN
SM5_BN
29
PGND
SM6_AP
SM6_AP
30
PGND
SM6_AN
SM6_AN
31
PGND
SM6_BP
SM6_BP
32
PGND
SM6_BN
SM6_BN
column B
column C
PGND
SM7_AP
SM7_AP
PGND
SM7_AN
SM7_AN
PGND
SM7_BP
SM7_BP
PGND
SM7_BN
SM7_BN
PGND
SM8_AP
SM8_AP
PGND
SM8_AN
SM8_AN
PGND
SM8_BP
SM8_BP
PGND
SM8_BN
SM8_BN
PGND
HT1_ON
HT1_ON
10
PGND
HT2_ON
HT2_ON
11
PGND
PGND
PGND
12
PGND
PUMP7
PUMP7
13
PGND
PUMP6
PUMP6
14
P+12V
PUMP5
PUMP5
15
P+12V
PUMP4
PUMP4
16
P+12V
PUMP3
PUMP3
17
P+12V
PUMP2
PUMP2
18
P+12V
PUMP1
PUMP1
19
V1
V2
V3
20
V4
V5
V6
21
V7
V8
V9
22
V10
V11
V12
23
V13
V14
V15
24
V16
V17
V18
25
V19
V20
V21
26
V22
V23
V24
27
V25
V26
V27
28
V28
V29
V30
5-14
Hardware System
29
V31
V32
V33
30
V34
V35
V36
31
V37
V38
V39
32
V40
NC
NC
Definition
Description
HT1_SA
HT1_SB
HT2_SA
HT2_SB
AMBI_SA
both
AMBI_SB
environment temperature
ends
of
thermistor
of
Connector J15 for sensors of volumetric board and pressure control board
Definition
Description
P+12V
PGND
DGND
POSI_PRESS
NEGA_PRESS
DGND
#VM_ON
#RBC_START
#RBC_STOP
10
#WBC_START
11
#WBC_STOP
12
D+5V
5-15
Hardware System
Table 5-22 Definition of J16 for mother board
Pin
Definition
Description
D+5V
#LIQ1
#LIQ2
#LIQ3
#LIQ4
#LIQ5
#LS_ON
GND
NC
10
NC
11
#LIQ6
12
GND
13
#LYSE_SENSOR
14
GND
15
#WASTE_SENSOR
16
GND
17
NC
18
NC
19
OP17_CTRL
Optics 17 control
20
OP17_STS
Optics 17 status
21
GND
22
GND
23
OP18_CTRL
Optics 18 control
24
OP18_STS
Optics 18 status
25
GND
26
GND
27
OP19_CTRL
Optics 19 control
28
OP19_STS
Optics 19 status
29
GND
30
GND
31
OP20_CTRL
Optics 20 control
32
OP20_STS
Optics 20 status
33
GND
34
GND
Position sensor has three connectors, all using double-row and 2mm connectors.
5-16
Hardware System
Connector type: HEADER WTB 2.0MM DIP2*12 TOP
Model: B24B-PHDSS-B (LF) (SN) 'JST'
Table 5-23 Definition of J18 for mother board
Pin
Definition
Description
OP1_CTRL
Optics 1 drive
OP1_STS
Optics 1 status
3,4
DGND
OP2_CTRL
Optics 2 drive
OP2_STS
Optics 2 status
7,8
DGND
OP3_CTRL
Optics 3 drive
10
OP3_STS
Optics 3 status
11,12
DGND
13
OP4_CTRL
Optics 4 control
14
OP4_STS
Optics 4 status
15,16
DGND
17
OP5_CTRL
Optics 5 drive
18
OP5_STS
Optics 5 status
19,20
DGND
21
OP6_CTRL
Optics 6 drive
22
OP6_STS
Optics 6 status
23,24
DGND
Definition
Description
OP7_CTRL
Optics 7 drive
OP7_STS
Optics 7 status
3,4
DGND
OP8_CTRL
Optics 8 drive
OP8_STS
Optics 8 status
7,8
DGND
OP9_CTRL
Optics 9 drive
10
OP9_STS
Optics 9 status
11,12
DGND
13
OP10_CTRL
Optics 10 drive
14
OP10_STS
Optics 10 status
15,16
DGND
17
OP11_CTRL
Optics 11 drive
5-17
Hardware System
18
OP11_STS
Optics 11 status
19,20
DGND
21
OP12_CTRL
Optics 12 drive
22
OP12_STS
Optics 12 status
23,24
DGND
Definition
Description
OP13_CTRL
Optics 13 drive
OP13_STS
Optics 13 status
3,4
DGND
OP14_CTRL
Optics 14 drive
OP14_STS
Optics 14 status
7,8
DGND
OP15_CTRL
Optics 15 drive
10
OP15_STS
Optics 15 status
11,12
DGND
13
OP16_CTRL
Optics 16
14
OP16_STS
Optics 16 status
15,16
DGND
17-24
NC
TOP PHSERIES
Definition
DM_ON
NC
DGND
Description
DC Motor drive
5-18
Hardware System
Table 5-27 Definition of J22 for mother board
Pin
Definition
Description
SM1_AP
SM1_AN
SM1-BP
SM1_BN
SM2_AP
SM2_AN
SM2-BP
SM2_BN
Definition
Description
SM3_AP
SM3_AN
SM3-BP
SM3_BN
SM4_AP
SM4_AN
SM4-BP
SM4_BN
Definition
Description
SM5_AP
SM5_AN
SM5-BP
SM5-BN
SM6_AP
SM6_AN
SM6-BP
SM6_BN
5-19
Hardware System
Table 5-30 Definition of J25 for mother board
Pin
Definition
Description
SM7_AP
SM7_AN
SM7-BP
SM7_BN
SM8_AP
SM8_AN
SM8-BP
SM8_BN
Definition
P+24V
HT1_ON
3,4
Description
Heater 1 drive
Heater 1 control
Definition
P+24V
HT2_ON
3,4
Description
Heater 2 drive
Heater 2 control
Connector J29, J30, J31, J32, J33 for pumps and valves
Definition
1~6
NC
PUMP7
P+12V
PUMP6
10
P+12V
Description
Pump 7 drive
Pump 6 drive
5-20
Hardware System
11
PUMP5
12
P+12V
13
PUMP4
14
P+12V
15
PUMP3
16
P+12V
17
PUMP2
18
P+12V
19
PUMP1
20
P+12V
Pump 5 drive
Pump 4 drive
Pump 3 drive
Pump 2 drive
Pump 1 drive
Definition
V1
P+12V
V2
P+12V
V3
P+12V
V4
P+12V
V5
10
P+12V
11
V6
12
P+12V
13
V7
14
P+12V
15
V8
16
P+12V
17
V9
18
P+12V
19
V10
20
P+12V
Description
Valve 1 drive
Valve 2 drive
Valve 3 drive
Valve 4 drive
Valve 5 drive
Valve 6 drive
Valve 7 drive
Valve 8 drive
Valve 9 drive
Valve 10 drive
Definition
V11
P+12V
Description
Valve 11 drive
5-21
Hardware System
3
V12
P+12V
V13
P+12V
V14
P+12V
V15
10
P+12V
11
V16
12
P+12V
13
V17
14
P+12V
15
V18
16
P+12V
17
V19
18
P+12V
19
V20
20
P+12V
Valve 12 drive
Valve 13 drive
Valve 14 drive
Valve 15 drive
Valve 16 drive
Valve 17 drive
Valve 18 drive
Valve 19 drive
Valve 20 drive
Definition
V21
P+12V
V22
P+12V
V23
P+12V
V24
P+12V
V25
10
P+12V
11
V26
12
P+12V
13
V27
14
P+12V
15
V28
16
P+12V
17
V29
Description
Valve 21 drive
Valve 22 drive
Valve 23 drive
Valve 24 drive
Valve 25 drive
Valve 26 drive
Valve 27 drive
Valve 28 drive
Valve 29 drive
5-22
Hardware System
18
P+12V
19
V30
20
P+12V
Valve 30 drive
Definition
V31
P+12V
V32
P+12V
V33
P+12V
V34
P+12V
V35
10
P+12V
11
V36
12
P+12V
13
V37
14
P+12V
15
V38
16
P+12V
17
V39
18
P+12V
19
V40
20
P+12V
Description
Valve 31 drive
Valve 32 drive
Valve 33 drive
Valve 34 drive
Valve 35 drive
Valve 36 drive
Valve 37 drive
Valve 38 drive
Valve 39 drive
Valve 40 drive
Definition
D+5V
D+5V
DGND
DGND
Hardware System
Connector type: HEADER WTB 4.2MM DIP2X5 TOP 5566SERIES
Model: 39-31-0108
Table 5-39 Definition of J35 for mother board
Pin
Definition
P+24V
P+24V
P+24V
P+12V
P+12V
PGND
PGND
PGND
PGND
10
PGND
Definition
P+12V
PGND
Definition
D+5V
P+12V
P+24V
DGND
PGND
PGND
5-24
Hardware System
Assembly drawing
5-25
Hardware System
JP1
JP2
No jumpers
3-4,
Drive board COM
Put jumpers on
pins 5-6
No jumpers
and 7-8
Data board UART1
Put
and 11-12.
1-2,,3-4,,5-6, and,7-8..
Put
COM
and 11-12.
9-10,11-12,13-14,and 15-16.
No jumpers.
Autoloader
board
jumpers
jumpers
on
on
pins
pins
and 15-16.
A male or female receptacle can be installed at J2. Four cable types can be supported, as
shown in the following table.
5-26
Hardware System
Table 5-43 COM port receptacle and cable selection
Connector
cable
Serial
DB9
male
cable
JP3
with
female
receptacle
(straight or 90
Serial
installation)
cable
with
female
female
receptacle
(straight or 90
installation)
JP4
No jumpers
autoloader board
Connect MCU JTAG of
drive board
7-8.
Note: the mother board does not provide a fixed connection to the JTAG port of the autoloader
MCU. If you need to update the autoloader MCU on the analyzer, connect the J11 and J3
receptacles using the cable dedicated for autoloader update before updating on PC using the
client update software. The dedicated cable is provided to the service and manufacture
personnel, not as an accessory of the analyzer.
5-27
Hardware System
5.1.4 Removal
Purpose
The mother board is unlikely to be damaged. But in case you need to replace it, follow the
steps below.
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
Removal
Follow these two procedures to disassemble the mother board:
5-28
Hardware System
2 Mother board
1. Use the 107 cross-headed screwdriver to remove the fourteen M3X6 cross-headed
panhead screws on the mother board.
2. Pull the mother board towards the panel by a certain distance, remove the LAN port
connector from the metal plate and take out the mother board.
2 Mother board
Hardware System
Installation
Install the mother board as per the above-mentioned procedures in the reverse order.
Note: Since the mother board has many connectors, pay attention to the number indicated on
the connector to avoid wrong connection. The design of the mother board has included certain
error-proof measures. Generally speaking, wrong connection will not lead to safety issues, yet
the whole unit cannot function properly
Verification
1. Power on the analyzer, the fan at the back of the analyzer should run normally. Use a
multimeter to measure the voltage of D+5V, P+12V, P+24V, A+/-12V, AC120V, and all
measurements should be within normal ranges.
2. Power indicators of the boards are on, showing that the mother board is functioning
correctly.
5.1.5 Troubleshooting
Since the function of the mother board is to realize power distribution and centralized wiring, a
failure in the mother board usually takes the form of hardware system failure.
Table 5-45 Troubleshooting
Failure
Abnormal
Cause
voltage
detected
cable
connected
not
Recommended Action
properly
to
power
connector
supply
Communication failure
Connector J4 abnormal..
Check connector J4
of autoloader board
Pressure
Volumetric abnormal
control
abnormal
5-30
Hardware System
abnormal, misinform
Laser not light or laser
Check connector J7
power
abnormal
current
detected
abnormal
or
work
abnormally.
Motor not active
Motor
drive
cable
not
properly connected .
Valve not active
Valve
drive
cable
and 25
not
properly connected.
Pump not active
Pump
drive
cable
not
not
properly connected
Temperature
fails.
control
Temperature
sensor
properly connected.
5-31
Hardware System
Monitoring voltage (including power voltage, WBC aperture voltage, RBC aperture voltage
and FS background voltage, also monitoring laser drive current and AD working status,
etc.)
Zapping apertures
AD conversion
Controlling interfaces for the indicator board and the key board
Providing communication interfaces for the autoloader board and the drive board
Controlling and monitoring interfaces for the volumetric board and the laser board
Block diagram
5-32
Hardware System
Gain control
RBC/PLT
sensor
RBC/PLT amplification
RBCPLT
RBC/PLT aperture
Constant current source
/zapping control
Monitoring
module
Gain control
WBC sensor
WBC
WBC aperture
WBC amplification
Digital
circuits
of data
board
Gain control
HGB sensor
HGB amplification
HGB
Driver control
HGB led constant current driver
A/D module
Gain control
SS preamplification board
SS amplification
FS preamplification board
FS amplification
FS
Gain control
SS preamplification board
SF amplification
Gain control
SS
FS base currrent
SF
5-33
Hardware System
Description
The A12V and AC120V analog power sent from the mother board are conditioned or
converted to be the required voltages for the board. The AC120V is used for zapping
apertures.
Monitoring voltage
The voltage of A12V power and VCONST, WBC aperture voltage, RBC aperture voltage, FS
blank voltage, laser drive current and AD working status are all monitored via AD.
WBC/RBC volume signals are collected and conditioned, and then sent to the AD converter.
The HGB current signals are converted into voltage signals through colorimetric method, then
after conditioning, they are sent to the AD converter.
The WBC DIFF signals including FS, SS and SF signal; SF signal reserved are first
collected and conditioned, and then sent to the AD converter.
The data board provides the constant current for the electrodes of the WBC bath and the
RBC/PLT bath and the HGB light.
Hardware System
The zapping voltage on-off, constant current supply/zapping switch and HGB light on-off are
controlled by the GPIO interface of CPU. The amplification factors of WBC DIFF, WBC,
RBC/PLT and HGB signal channels are set by the FPGA as required.
Zapping aperture
If the analyzer has been running for a certain period or the WBC/RBC bath aperture clogs, the
zapping function shall be performed to clean up the aperture by high temperature.
AD conversion
The cell signals (WBC, RBC, PLT and WBC DIFF signals), voltage monitoring signals and
HGB signals are converted into digital signals, and then sent to FPGA for further processing.
The digital part:
Sending control command to the driver board and receiving its response.
Baud rate: 38400bps
Data bit: 8bit
Stop bit: 1bit
Start bit: 1bit
Parity: even
Sending control command to the autoloader board via serial port and receiving its response.
Baud rate: 38400bps
Data bit: 8bit
Stop bit: 1bit
Start bit: 1bit
Parity: even
The IO port for the driver board and autoloader board upgrading
By simulating the JTAG sequence through the CPU IO port, the driver board and the
autoloader board can be upgraded respectively.
The status indicator and the buzzer on the indicator board are controlled by the CPU IO port.
Enabling the laser control board and detecting laser diode current
Receiving the key events (including the aspiration key and the keypad).
5-35
Hardware System
Detecting the open/closed status of the side door and laser shielding box
Receiving the analog signals from the pre-amplification board and the aperture electrodes
The network interface is introduced by the mother board and connected to PC by the cross
network cable, and capable of program downloading and data transmission, etc.
The BDM interface of CPU, which is used for detecting hardware, downloading program and
writing the FLASH, is not available for customers.
It debugs FPGA and downloads FPGA program. It is not available for customers.
Interface definition
The data board is a plug-in board. It provides 9 connectors: the connectors for aperture
electrodes, the connector to connect the digital part to the mother board, the connector to
connect the analog part to the mother board, the connectors for debugging and the reserved
connectors. See Figure 5-9 for the layout of the data board.
See the following table for the function of each connector.
Table 5-46 Interfaces of data board
Connector
J1
J2
J3
J7
Number of
Function
Connects
the
RBC/PLT
Note
pins
aperture
electrode
Connects the WBC aperture electrode
Connects the digital part to the mother
board
Connects the analog part to the
mother board
3
3
96
96
/
/
/
/
J8
10
J9
Reserved
20
J10
Reserved
40
J11
Reserved
14
J13
26
5-36
Hardware System
Definition of J1
Name
Note
SHELL
Shielding ground
RHOLE_A
RBC/PLT signals
RHOLE_B
Definition of J2
Name
Note
WHOLE _B
WHOLE_A
WBC signals
SHELL
Shielding ground
Definition of J3
J3 is the signal interface for the digital part of data board and mother board, and is European
96 pin connector.
5-37
Hardware System
Table 5-49 Definition of J3
Pin
A1
A2
A3
A4
A5
Definition
D+5V
D+5V
D+5V
D+5V
D+5V
Function
Pin
Definition
Function
Power supply
B17
JTAG_TCK_
AS
board
JTAG_TO_A
JTAG
autoloader board
JTAG_FROM
JTAG
_AS
autoloader board
UART1_TO_
Reserved
DAT+
port sending
UART1_TO_
Reserved
DAT-
port sending
Power supply
B18
Power supply
B19
Power supply
B20
Power supply
B21
sending
of
receiving
debug
debug
of
serial
serial
A6
DGND
Signal ground
B22
DGND
Signal ground
A7
DGND
Signal ground
B23
DGND
Signal ground
A8
DGND
Signal ground
B24
DGND
Signal ground
A9
DGND
Signal ground
B25
#VMCTRL
A10
DGND
Signal ground
B26
#RBC_STAR
Start
metering
#RBC_STOP
Stop
A11
DGND
Signal ground
B27
RBC
volume
RBC
volume
WBC
volume
WBC
volume
metering
A12
TP_TX+
Positive sending
terminal
B28
of
#WBC_STAR
Start
metering
#RBC_STOP
Stop
network
A13
TP_TX-
Negative sending
terminal
B29
of
metering
network
A14
DGND
Signal ground
B30
DGND
Signal ground
A15
DGND
Signal ground
B31
#LASER_CT
RL
A16
JTAG_TMS
JTAG
mode
B32
DGND
Signal ground
signal
A17
JTAG_TCK
C1
D+5V
Power supply
A18
JTAG_TO_
JTAG sending of
C2
D+5V
Power supply
DRV
drive board
JTAG_TO_
JTAG receiving of
C3
D+5V
Power supply
DAT
drive board
A20
DGND
Signal ground
C4
D+5V
Power supply
A21
DGND
Signal ground
C5
D+5V
Power supply
A22
UART0_TO
Serial
C6
DGND
Signal ground
A19
port
5-38
Hardware System
_DRV
sending of drive
board
A23
UART0_TO
Serial
port
_DAT
receiving of drive
C7
DGND
Signal ground
board
A24
DGND
Signal ground
C8
DGND
Signal ground
A25
DGND
Signal ground
C9
DGND
Signal ground
A26
DGND
Signal ground
C10
DGND
Signal ground
A27
DGND
Signal ground
C11
DGND
Signal ground
A28
DGND
Signal ground
C12
TP_RX+
Positive
receiving
terminal of network
A29
DGND
Signal ground
C13
TP_RX-
Negative
receiving
terminal of network
A30
DGND
Signal ground
C14
DGND
Signal ground
A31
DGND
Signal ground
C15
DGND
Signal ground
A32
DGND
Signal ground
C16
UART3_TO_
PC communication serial
PC
port sending
UART3_TO_
PC communication serial
DAT
port receiving
B1
D+5V
Power supply
C17
B2
D+5V
Power supply
C18
DGND
Signal ground
B3
D+5V
Power supply
C19
DGND
Signal ground
B4
D+5V
Power supply
C20
UART1_TO_
AS+
port sending
UART1_TO_
AS-
port receiving
B5
D+5V
Power supply
C21
B6
DGND
Signal ground
C22
DGND
Signal ground
B7
DGND
Signal ground
C23
DGND
Signal ground
B8
DGND
Signal ground
C24
Aspiration
SUCK_KEY
input
key
control
B9
DGND
Signal ground
C25
DGND
Signal ground
B10
DGND
Signal ground
C26
BUZ
B11
DGND
Signal ground
C27
#COUNT_KE
Y
B12
PWFBOUT
Network
power
C28
FAULT_J
supply feedback
B13
DGND
Signal ground
C29
READY_J
B14
DGND
Signal ground
C30
OPTI_DOOR
Laser
shielding
monitoring input
5-39
box
Hardware System
B15
B16
DGND
Signal ground
mode
C31
JTAG_TMS
JTAG
of
_AS
autoloader board
C32
#RIGHT_DO
Right
door
status
OR
monitoring input
#INSERT_KE
Definition of J7
J7 is the signal interface for the analog part of data board and mother board, and is European
96 pin plug.
Table 5-50 Definition of J7
Pin
Name
Note
Pin
Name
Note
A1
AGND
Analog ground
B17
HGB_IN
A2
AGND
Analog ground
B18
AGND
Analog ground
A3
AGND
Analog ground
B19
HGB+
HGB
light
drive
positive
drive
negative
electrode
A4
AGND
Analog ground
B20
HGB-
HGB
light
electrode
A5
AGND
Analog ground
B21
A-12V
A6
AGND
Analog ground
B22
A-12V
A7
AGND
Analog ground
B23
A+12V
A8
AGND
Analog ground
B24
A+12V
A9
AGND
Analog ground
B25
A+12V
A10
AGND
Analog ground
B26
NC
A11
AGND
Analog ground
B27
NC
A12
AGND
Analog ground
B28
NC
A13
AGND
Analog ground
B29
NC
A14
AGND
Analog ground
B30
AC120V_
B
A15
AGND
Analog ground
B31
NC
A16
AGND
Analog ground
B32
NC
A17
AGND
Analog ground
C1
AGND
Analog ground
A18
AGND
Analog ground
C2
AGND
Analog ground
A19
AGND
Analog ground
C3
AGND
Analog ground
A20
AGND
Analog ground
C4
AGND
Analog ground
A21
AGND
Analog ground
C5
AGND
Analog ground
A22
AGND
Analog ground
C6
AGND
Analog ground
A23
AGND
Analog ground
C7
AGND
Analog ground
A24
AGND
Analog ground
C8
AGND
Analog ground
A25
AGND
Analog ground
C9
AGND
Analog ground
5-40
Hardware System
A26
NC
C10
AGND
Analog ground
A27
NC
C11
AGND
Analog ground
A28
NC
C12
AGND
Analog ground
A29
NC
C13
AGND
Analog ground
A30
NC
C14
AGND
Analog ground
A31
NC
C15
AGND
Analog ground
A32
AC120V_
C16
AGND
Analog ground
C17
AGND
Analog ground
C18
AGND
Analog ground
A
B1
AGND
Analog ground
B2
LASER_I
Laser
monitoring input
B3
AGND
Analog ground
C19
AGND
Analog ground
B4
AGND
Analog ground
C20
AGND
Analog ground
B5
AGND
Analog ground
C21
A-12V
B6
AGND
Analog ground
C22
A-12V
B7
AGND
Analog ground
C23
A+12V
B8
AGND
Analog ground
C24
A+12V
B9
AGND
Analog ground
C25
A+12V
B10
AGND
Analog ground
C26
NC
B11
FS_IN
FS signals input
C27
NC
B12
AGND
Analog ground
C28
NC
B13
SS_IN
SS signals input
C29
NC
B14
AGND
Analog ground
C30
NC
B15
SF_IN
SF signals input
C31
NC
B16
AGND
Analog ground
C32
NC
current
Definition of J8
Name
Note
FPGA_TCK
Clock
GND
Signal ground
FPGA_TDO
Data output
VDD
Power supply
FPGA_TMS
VDD
Power supply
NC
NC
5-41
Hardware System
FPGA_TDI
Data input
10
GND
Signal ground
Definition of J9
Definition of J10
Definition of J11
Definition of J13
Definition
Function
NC
BKPT#
Breakpoint setup
GND
Signal ground
DSCLK
GND
Signal ground
TCK
BDM_RSTI
BDM reset
DSI
Serial input
VDD
Power supply
10
DSO
Serial output
11
GND
Signal ground
12
PSTDATA7
13
PSTDATA6
14
PSTDATA5
15
PSTDATA4
16
PSTDATA3
17
PSTDATA2
18
PSTDATA1
19
PSTDATA0
20
GND
Signal ground
21
NC
22
NC
23
GND
Signal ground
Configuration data
5-42
Hardware System
24
PSTCLK
Processor clock
25
NC
26
MCF_TA#
Transmission response
Assembly drawing
5-43
Hardware System
5-44
Hardware System
Indicator
Function description
D17
D20
D21
D22
D39
D40
D41
D42
D43
10
D44
11
D45
12
D46
13
D47
14
D48
15
D49
16
D51
5-45
Hardware System
Table 5-54 Test points on data board
No.
Test point
Signal
Function description
under test
+12V
AVCC
-12V
AVSS
+5V
AVDD
-5V
AVEE
VR_MON
+2.5V
VR_H
+2.5V
+56V
VCONST
+12VM
-12VM
10
+56VM
VCONSTM
11
RHOLE
RHOLE
12
WHOLE
WHOLE
13
FSBASE
FSBASE
14
LASER
LASER
15
HGB1
HGB1
16
NHGB
#HGBLED
17
HGB
HGB
18
NBURN
#BURN
Zapping control
19
PULSE
PULSE
20
FS_IN
FS_IN
21
FS1
FS1
22
FS
FS
23
SS_IN
SS_IN
24
SS1
SS1
25
SS
SS
26
SF_IN
SF_IN
27
SF1
SF1
28
SF
SF
29
NCONST
#CONST
30
NSEL_R
#SELECT_
A+12V_MO
N
A-12V_MO
N
Monitor drive voltage of constant current
source of electrode
Pulse
control
signals
that
produce
zapping voltage
5-46
Hardware System
RBC
selection control
#SELECT_
WBC
selection control
31
NSEL_W
32
RBC1
RBC1
33
RBC2
RBC2
34
RBC3
RBC3
35
RBC
RBC
36
PLT1
PLT1
37
PLT2
PLT2
38
PLT
PLT
39
WBC1
WBC1
40
WBC2
WBC2
41
WBC3
WBC3
42
WBC
WBC
43
HCLK
HCLK
44
HGND
HGND
Analog ground
45
RBCF
RBCF
46
VR_R
VR_R
47
RAGND
RAGND
Analog ground
48
RCLK
RCLK
49
WBCF
WBCF
50
VR_W
VR_W
51
WAGND
WAGND
Analog ground
52
WCLK
WCLK
53
PLTF
PLTF
54
VR_P
VR_P
55
PAGND
PAGND
Analog ground
56
PCLK
PCLK
57
MCLK
MCLK
58
FSF
FSF
AD input from FS
59
FSCLK
FSCLK
AD clock signals of FS
5-47
Hardware System
60
SSF
SSF
AD input from SS
61
SSCLK
SSCLK
AD clock signals of SS
62
SFF
SFF
AD input from SF
63
SFCLK
SFCLK
AD clock signals of SF
64
TP8
MCF_FBAD
65
TP9
66
TP37
67
TP45
VCC
68
TP46
VDD
69
TP47
D_1V5
70
TP48
D_1V8
71
TP57
D_1V2_FG
72
TP71
D_2V5
73
TP74
#MCF_CS1
74
TP75
MCF_R/#W
75
TP76
MCF_FBAD
76
TP78
D_1V25VTT
77
TP79
#MCF_CS0
78
TP80
FPGA_DDR
79
TP81
80
TP82
81
TP83
82
TP85
83
TP86
84
TP87
85
TP95
86
TP96
1
MCF_FBAD
2
FPGA_DDR
_VRF
PA
_2V5
FPGA_DDR
_VTT
MCF_FBAD
5
D_1V25VR
F
MCF_FBAD
5
#MCF_OE
MCF_TS
MCF_FBAD
3
#MCF_RST
5-48
Hardware System
I
87
TP101
88
TP102
89
TP103
90
TP104
91
TP105
92
TP106
#MCF_IRQ
1
#MCF_IRQ
2
#MCF_IRQ
3
#MCF_IRQ
5
#MCF_IRQ
6
#MCF_IRQ
AGND, TP10-21, TP38, TP44, TP70, TP73, TP77, TP88, TP90, TP92-94 and TP107-116 are
connected to the GND network and usually used as the connecting point of the oscilloscope
probe grounded end.
Function
Manually reset the main CPU
Purpose
Damaged data board needs to be replaced by a new one..
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
Disassembly
Data board disassembly consists of the following two main procedures:
5-49
Hardware System
data board
(with washer)
3---Data board
4---Hand-tightened screws
1. Use the 107 cross-headed screwdriver to remove the 5 cross-headed panhead screws
(M3X6) from the data board.
5-50
Hardware System
2. Remove the data board from the bracket.
3. Use the 107 cross-headed screwdriver to remove the 2 panhead screws (M3X8) and 6
socket cap screws (M3X12) from the lower cover of the shielding box.
panhead
screw
2---Data board
Assembly
Assemble the data board as per the above-mentioned steps in the reverse order.
Verification
1. Power indicators D20, D17, D22, D21 and D51 turns on.
2. Indicator D49 turns on after FPGA initialization.
3. Indicator D47 and D48 turn on and D45 flashes after network is enabled.
5.2.5 Troubleshooting
The errors analysis and the corresponding troubleshooting of the analog part on data board
are listed in the following table. Most of the errors can be tested and determined by testing the
corresponding test points with a multimeter. A measurement value within the normal range
indicates the circuit or component is in good working condition. Otherwise, be sure to find out
5-51
Hardware System
the particular error according to the certain symptom and remove it accordingly.
Table 5-56 Troubleshooting of analog part
No.
1
Error
12V
power
supply error
Character
Possible Cause
Improper
+12V
connection
test
point
cable
with
of 120.6V; or the
or
ripple
caused
noise
is
Recommended action
Reconnect
the
cable
or
disconnection
by
broken cable
Improper
12V
by
the
board
mother
or
the
board
power board
Inductor
L16
capacitor
or
C111,
Re-solder
or
change
the
component
-12V
power
supply error
Improper
-12V
connection
test
point
cable
with
of -120.6V; or the
or
ripple
caused
noise
is
Reconnect
the
cable
or
disconnection
by
broken cable
Improper
-12V
by
the
board
mother
or
the
board
power board
Inductor
capacitor
L18
or
C112,
5-52
Re-solder
component
or
change
the
Hardware System
3
+5V
power
supply error
+5V
circuit
rosin
of +50.25 V
supplys
re-solder
impedance to the
component.
test
point
is
ground
is
decreased;
or
or
change
the
-5V
power
supply error
-5V
circuit
rosin
of -50.25 V
supplys
re-solder
impedance to the
component.
test
point
is
ground
is
decreased;
or
or
change
the
+2.5V reference
power
VR_MON or VR_H
power
supply
re-solder
works
normally,
the
supply
error
range
of
2.50.125 V
or
change
the
component
occurred probably
because
power
are
damaged or not
properly soldered.
6
+56
current
power
error
constant
drive
works improperly
supply
56V
voltage-regulator
diode is damaged
or
not
soldered.
5-53
properly
Re-solder
component
or
change
the
Hardware System
7
+56V monitoring
voltage error
supply
components;
of 1.370.2V
problem occurred
works
re-solder
or
probably because
something
wrong
is
with
the
monitoring circuit..
8
+12V monitoring
voltage error
is
properly
soldered;
the
of 1.8V-2.2V
power
not
supplys
impendence to the
ground
re-solder
or
change
the
component.
is
decreased;
Something
wrong
is
with
the
power IC or the
corresponding AD.
9
-12V monitoring
voltage error
-12VM
is
components,
test
point
not
properly
soldered;
of 2.2V-2.6V
power
re-solder
or
the
supplys
impendence to the
ground
normally
is
decreased;
Something
wrong
is
with
the
power IC or the
corresponding AD.
10
RBC
aperture
Constant
current
voltage
aperture
source
control
monitoring error
signals abnormal,
are
of 10V-14V
56V
foregoing troubleshooting to
voltage-regulator
diode abnormal or
circuit.
voltage
normal.
See
the
Check
wired; clogging.
perform
the
procedure
5-54
the
bath
wiring;
unclogging
Hardware System
Monitoring
circuit
error
Components
the
of
monitoring
circuit
are
not
properly soldered,
or
the
corresponding AD
works abnormally.
11
WBC
aperture
Same as point 10
Same as point 10
Same as point 10
HGB
background
the gain
voltage
abnormal
(4.2V-4.8V)
voltage
monitoring error
12
of HGB channel
The
Check
corresponding AD
corresponding
works abnormally,
normally.
or
the
error
message
is
whether
AD
the
works
accidentally
triggered
by
improper wiring.
13
FS background
voltage
dirty, or something
monitoring error
range (0-400mv)
corresponding
monitoring circuit
error.
14
Laser
drive
diode
Something
current
wrong
laser
of 1.0-3.0V.
by
monitoring error
with
the
control board
is
the
laser
control
and
5-55
board
Hardware System
Data
board
monitoring circuit
amplifier
error,
the
corresponding AD
error
U38
and
the
work normally.
or
connection
cable
abnormal
15
RBC
aperture
zapping error
No sound is heard
Something
wrong
produced
when
is
the
zapping
voltage
supplied
by
the
power board, or
data
effective.
with
board
that
controls zapping.
16
WBC
aperture
Same as point 15
Same as point 15
Same as point 15
No
No WBC/RBC/PLT
WBC/RBC/PLT
analog
part
result
displays ***
channel error, AD
collection error or
no
algorithm
identification error
zapping error
17
signal
is
detected,,
WBC/RBC/PLT
WBC/RBC/PLT
Bad
background
background
constant
abnormal
abnormal
shielding,
current
source abnormal,
analog
grounding,
channel
check
resistor-capacitor
components
components
incorrect
abnormal
or
(such
as
and
5-56
Hardware System
Table 5-57 Troubleshooting of digital part
No.
1
Error
System startup failed
the
operating
system
to
fail
to
be
U66 damaged.
Solutions: check and make sure the connection is
proper; check whether the interface signals are
normal by an oscilloscope.
U74 damaged.
check
the
interface
signals
by
an
oscilloscope.
5
U67 damaged.
Solutions: check and make sure the connection is
proper;
check
oscilloscope.
5-57
the
interface
signals
by
an
Hardware System
6
U67 damaged.
proper;
oscilloscope.
control.
5-58
check
the
interface
signals
by
an
Hardware System
Serial communication
Temperature control
Pressure control
Block diagram
2-channel temperature
measurement
2-channel pressure measurement
3-channel power voltage
measurement
Temperature
Pressure
Power
Voltage
(anolog
signal)
Databoard
UART
RD
A/D[7:0]
IN
A[15:8]
EN
C8051F020
TDI
CLK
TDO
F_INT
CLK
F_NCONFIG
LATCH
NCSO
BN
HOLD
RXD
MOTOR
A3979
SLEEP
WR
TXD
AN
MS2
STEP
ALE
TMS
Databoard
JTAG
A
MS1
EP1C6Q240C8
DATA
74HC595
+
IRLML2502
SV1~SV40
40
DCLK
ASDO
M_SEN
Pump
Heater
HOA0880T51
CD74HC14
20-channel motor position sensor
detection
EPCS1S18N
7-channel high power pump & valve
control
2-channel heater control
LSEN/SWITCH
74LVCR2245
5-59
Hardware System
Description
Serial communication
The main control unit of the drive board is a MCU, which has two asynchronous serial
communication interfaces (UART). UART0 is used for asynchronous serial communication
with the data board. It receives and analyzes control commands sent by the data board, and
responds accordingly. Communication parameters:
Baud Rate: 38400bps
Start bit: 1bit
Data bit: 8 bits
Stop bit: 1bit
Parity: even
UART1 is reserved.
The drive board controls stepping motors through an FPGA. After output by the FPGA, the
control signal is first sent to isolator 74AHCT245, and then to controller A3979 to drive the
motor. The drive board drives 8 stepping motors: the sample syringe motor, the sample
injection syringe motor, the sheath syringe motor, the diluent syringe motor, the lyse syringe
motor, the horizontal motor and the vertical motor.
The drive board provides the drive control for 40 valves and 7 pumps and actually drives 34
valves, 1 pressure pump and 2 vacuum pumps (also be used as the waste pump). The MCU
writes control information of valve SV1-SV40 into the valve buffer of the FGPA. Based on the
data, the FPGA outputs valve control signals through the serial-parallel conversion chip
74HC595, and drive the valves through the MOS transistor IRLML2502. The MCU first sends
the control information of PUMP1-PUMP3 to isolator 74AHCT245, and then to the drive chip
ULN2068 to control the 3 pumps respectively.
Temperature Control
The temperature control circuit keeps temperature of the reaction bath (DIFF bath) and the
optical system constant. By comparing the temperature measurements and the target values,
the MCU turns on/off the heater to control the temperature of each channel. The MC sends
heater control signals to isolator 74AHCT245, then to driver FDS6670A to control the two
heaters.
The pressure control circuit controls the pressure of the vacuum chamber and the pressure
chamber. The pressure signals (voltage signals) sent by the volumetric board are measured by
5-60
Hardware System
the drive board. Through voltage follower LM358, the signals are then sent to the ADC pin of
the MCU for AD conversion and corresponding operation. Then, the control signals are output
to turn on/off the pressure pump or the vacuum pump, thus, the pressure of each chamber is
adjusted.
The drive board detects the ambient temperature and the voltage of power supply. The mother
board sends the inquiry command to obtain the corresponding system status parameter.
Ambient temperature sensor is located on the interface board. After I/V conversion and
amplification, the signals outputted by the temperature sensor are sent to the MCU for AD
conversion and operation, then the temperature value is obtained.
The voltage detection circuit detects all the power supplies (24V, 12V and 5V) on the drive
board.
The reagent status is detected by the passive float switch. There are totally 8 reagent detection
channels on the drive board as designed, and 6 channels of them are in use to detect the
following reagents: LEO1, LEO2, LH, cleanser, diluent and waste.
Interface definition
The drive board connects the motherboard via the 3 DIN41612 plugs and 3 DIN41612
receptacles located on the two boards respectively. All the drive-board-relating peripherals
(including valves, pumps, heaters, motors and sensors etc.) are connected to the motherboard
directly. The connectors layout and the corresponding connections are shown in the figure
below.
5-61
Hardware System
Position sensor
JTAG
UART
Pressure
Valve
PUMP
Heater
2-channel step-motor
6-channel step-motor
+24V
+5V
+12V
Base board
PB2 Socket
PB1 Socket
PB3 Socket
J4
J5
Board mounting
bracket & guide
channel
J3
Socket 96Pin
96Pin
Driver board
J9
J1
Support board
J2
J10 J8
J3
Number of
Function
Note
pins
10
10
96
interface
of
valves,
pumps
and
detection
and
heaters
J4
96
J5
96
J8
J9
J10
Communication
Debug
Interface
MCU Debug Interface
Communication
4
10
Debug
Interface
5-62
Sensor
communication interface
Motor control interface
Not used on complete
device
Used when MCU first
downloading
Not used on complete
device
Hardware System
Definition of J1
Name
Pin
Name
F_DCLK
AGND
F_CONFDONE
VDD
F_CONFIG
F_NCE
F_DATA
F_NCSC
F_ASDO
10
GND
Definition of J2
J3 connects to the PB3 connector of the motherboard, and is the interface used for valves,
pumps and heaters control.
Table 5-60 Valve/pump interface- Definition of J3
Pin
Definition
Note
1
2
3
4
5
6
PGND
Power ground
12V0
19
SV1
20
SV4
7
8
9
10
11
12
13
14
15
16
17
18
5-63
Hardware System
21
SV7
22
SV10
23
SV13
24
SV16
25
SV19
26
SV22
27
SV25
28
SV28
29
SV31
30
SV34
31
SV37
32
SV40
33
M7-A
34
M7-AN
35
M7-B
36
M7-BN
37
M8-A
38
M8-AN
39
M8-B
40
M8-BN
41
HT1-DRV
42
HT2-DRV
43
PGND
Power ground
44
PUMP7
45
PUMP6
46
PUMP5
47
PUMP4
48
PUMP3
49
PUMP2
50
PUMP1
51
Sv2
5-64
Hardware System
52
SV5
53
SV8
54
SV11
55
SV14
56
SV17
57
SV20
58
SV23
59
SV26
60
SV29
61
SV32
62
SV35
63
SV38
64
NC
Empty
65
M7-A
66
M7-AN
67
M7-B
68
M7-BN
69
M8-A
70
M8-AN
71
M8-B
72
M8-BN
73
HT1-DRV
74
HT2-DRV
75
PGND
Power ground
76
PUMP7
77
PUMP6
78
PUMP5
79
PUMP4
80
PUMP3
81
PUMP2
82
PUMP1
5-65
Hardware System
83
SV3
84
SV6
85
SV9
86
SV12
87
SV15
88
SV18
89
SV21
90
SV24
91
SV27
92
SV30
93
SV33
94
SV36
95
SV39
96
NC
Empty
Definition of J4
J4 connects to the PB1 connector of the motherboard, and is the interface used for sensor
detection and communication.
Table 5-61 Sensors/Communication interfaces-Definition of J4
Pin
Name
Note
PRTO_T1
PRTO_T2
PRESSURE_1ST_A
GND
GND
VCC
5V power supply
17
GND
GND
18
LIQ_SIN1
4
5
6
7
8
9
10
11
12
13
14
15
16
5-66
Hardware System
19
LIQ_SIN3
20
LIQ_SIN5
21
LIQ_SIN7
22
LS_CTRL
23
GND
Digital ground
24
VC_SIN17
25
VC_SIN18
26
VC_SIN19
27
VC_SIN20
28
VC_SIN1
29
M_SIN2
30
VC_SIN4
31
M_SIN5
32
VC_SIN7
33
PRTO_REF
34
PRTO_REF
35
PRESSURE_2ST_A
GND
Digital ground
VCC
5V power supply
49
GND
Digital ground
50
LIQ_SIN2
51
LIQ_SIN4
52
LIQ_SIN6
36
37
38
39
40
41
42
43
44
45
46
47
48
5-67
Hardware System
53
54
55
LIQ_SIN8
GND
Digital ground
Receiving end feedback signal of position sensor 17
56
M_SIN17
57
M_SIN18
58
M_SIN19
59
M_SIN20
60
M_SIN1
61
VC_SIN3
62
M_SIN4
63
VC_SIN6
64
M_SIN7
65
AMBINET_T_A
GND
Digital ground
VCC
5V power supply
81
GND
Digital ground
82
TMS
83
TCK
(reserved)
Receiving end feedback signal of position sensor 18
(reserved)
Receiving end feedback signal of position sensor 19
(reserved)
Receiving end feedback signal of position sensor 20
(reserved)
Receiving end feedback signal of sampling syringe initial
position sensor (OP 1)
Sending end power signal of sheath syringe initial position
sensor (OP 3)
Receiving end feedback signal of lyse syringe initial position
sensor (OP 4)
Sending end power signal of relieve valve position sensor (OP
6)
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
5-68
Hardware System
84
TDI
85
TDO
GND
Digital ground
88
UP_TX
89
UP_RX
GND
Digital ground
86
87
90
91
92
VC_SIN2
93
M_SIN3
94
VC_SIN5
95
M_SIN6
96
VC_SIN8
Definition of J5
J5 connects to the PB2 connector of the motherboard, and is the interface used for the
stepping motor control and the position sensor signal detection.
Table 5-62 Stepping motor control interface-definition of J5
Pin
Definition
Note
M_SIN8
VC_SEN10
M_SIN11
VC_SEN13
Sending end power signal of WBC bath position sensor (OP 13)
M_SIN14
VC_SEN16
GND
Digital ground
NC
Empty
24V0
10
11
12
13
5-69
Hardware System
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
PGND
Power ground
33
VC_SEN9
34
M_SIN10
35
VC_SEN12
36
M_SIN13
37
VC_SEN15
38
M_SIN16
39
GND
Digital ground
40
NC
Empty
41
M1_A
42
M1_AN
43
M1_B
44
M1_BN
29
30
31
32
Hardware System
45
M2_A
46
M2_AN
47
M2_B
48
M2_BN
49
M3_A
50
M3_AN
51
M3_B
52
M3_BN
53
M4_A
54
M4_AN
55
M4_B
56
M4_BN
57
M5_A
58
M5_AN
59
M5_B
60
M5_BN
61
M6_A
62
M6_AN
63
M6_B
64
M6_BN
5-71
Hardware System
6, reserved)
65
M_SIN9
66
VC_SEN11
67
M_SIN12
68
VC_SEN14
69
M_SIN15
70
DCM
71
GND
Digital ground
72
NC
Empty
73
M1_A
74
M1_AN
75
M1_B
76
M1_BN
77
M2_A
78
M2_AN
79
M2_B
80
M2_BN
81
M3_A
82
M3_AN
83
M3_B
84
M3_BN
85
M4_A
86
M4_AN
Hardware System
87
M4_B
88
M4_BN
89
M5_A
90
M5_AN
91
M5_B
92
M5_BN
93
M6_A
94
M6_AN
95
M6_B
96
M6_BN
Definition of J8
Definition of J9
Name
Pin
Name
VDD
GND
GND
TCK
TMS
TDO
TDI
NC
GND
10
NC
Definition of J10
5-73
Hardware System
Assembly drawing
5-74
Hardware System
Note
FPGA configuration status indicator. It turns on during FPGA configuration.
FPGA working status indicator. It flashes every 1s when FPGA works
normally.
24V power supply (24V0) status indicator. It turns on when the 24V power
supply is normal.
3.3V power supply (VDD) status indicator. It turns on when the 3.3V power
supply is normal.
12V power supply (12V0) status indicator. It turns on when the 12V power
supply is normal.
5V power supply (VDD) status indicator. It turns on when the 5V analog
power supply is normal.
D65
MCU working status indicator. It flashes every 1s when MCU works normally.
D66
D67
Name
Pin
Note
TP1
PIN26 of U2
TP2
PIN28 of U2
TP3
PIN228 of U2
TP4
PIN50 of U2
TP5
PIN20 of U2
TP6
PIN73 of U2
5-75
Hardware System
7
TP7
PIN11 of U2
TP8
1V5
TP9
SIO_IN
10
TP10
SIO_CLK
11
TP11
SIO_EN
12
TP12
SIO_LATCH
13
TP13
PIN17 of U10
14
TP14
PIN15 of U9
15
TP16
PIN15 of U21
16
TP17
PIN13 of U20
17
TP18
PIN17 of U20
18
TP19
PIN17 of U25
19
TP20
PIN16 of U26
20
TP21
PIN11 of U10
21
TP22
VDD
22
TP23
23
TP24
24
TP25
1V5
25
TP26
PIN1 of U32
26
TP27
ADC_VCC
27
TP28
ADC_12V
28
TP29
ADC_24V
29
TP30
PIN1 of U33
GND
of
+1.5VDC
power
supply
(1.50.075V)
of
+3.3VDC
power
supply
+1.5VDC
power
supply
detection
signals
(3.30.15V)
Digital ground
Voltage
of
(1.50.075V)
Ambient
temperature
(voltage) AMBINET_T
Detection signals of VCC+5VDC after
voltage-dividing 2.000.15V
Detection signals of 12V0(+12VDC) after
voltage-dividing 2.130.15V
Detection signals of 24V0(+24VDC) after
voltage-dividing 2.230.15V
Pressure
5-76
chamber
detection
signals
Hardware System
(voltage) PRESSURE_1ST
30
TP31
PIN7 of U33
31
TP34
PIN2 of U37
34
TP35
PIN100 of U35
35
TP36
PIN26 of U35
36
TP37
PIN99 of U35
37
TP38
PIN57 of U35
38
TP39
PIN6 of U45
39
TP40
PRTD_REF
40
TP41
PIN2 of U45
41
TP42
PIN3 of U45
42
TP43
PIN8 of U22
43
TP44
PIN8 of U23
44
TP45
PIN8 of U24
45
TP46
PIN8 of U29
46
TP47
PIN8 of U28
47
TP48
PIN8 of U27
48
TP49
PIN8 of U11
49
TP50
PIN8 of U13
Vacuum
chamber
detection
signals
(voltage) PRESSURE_2ST
Reference voltage of MCU analog-to-digital
module REFIN2.500.006V
Voltage of MCU digital-to-analog output
channel 0
MCU 25.5454MHz clock input signals
CLKIN
Voltage of MCU digital-to-analog output
channel 1
MCU system clock output signals SYSCLK
Amplifier output signals of the temperature
collection module TEMPER_AD
Basic voltage of the amplifier input signals
of the temperature collection module
Reference voltage of temperature collection
module amplifier
Input voltage of temperature collection
module amplifier
Driving reference voltage of sample syringe
motor VREF1
Driving
reference
voltage
of
sample
Key definition
The reset switch S1 on the drive board is used to reset the drive board manually. Press the
5-77
Hardware System
reset switch to reset the MCU and FPGA.
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
Disassembly
Drive board disassembly consists of the following two main procedures:
4. Use both of your hands to flip outward the two latches on both sides of the drive board,
and then draw the drive board out.
5-78
Hardware System
2---Drive board
1. Use the 107 cross-headed screwdriver to remove the 5 cross-headed panhead screws
(M3X6) from the drive board.
2---Bracket
panhead
screw
Hardware System
Assembly
Please assemble the data board as per the above-mentioned steps in the reverse order.
Be sure to shut down the analyzer and cut off the power supply before
disassembling/assembling the board.
Verification
1. Check and make sure that all the components are properly connected to the drive board.
2. First ensure the power supply is cut off, then assemble the drive board and motherboard
properly, then power on the analyzer.
3. Check if the indicators on the drive board are in the following status: 1) D49, D50 and D51
turn on; 2) D2 and D65 flash. You can also check the indicators in the self-test screen of
the analyzer. If the self-test is passed, it means the driver board is replaced successfully;
otherwise, you should troubleshoot the board.
4. Readjust the sample probe position as per Section 6.10.1 and save the result.
5.3.5 Troubleshooting
Hardware System
Only when you make sure that nothing is wrong with the connections can you remove the error
according to the recommended action in the following table. If the error still exists after the
error causes are found and new components are replaced, you need to replace the drive
board.
The error codes, error names and recommended solutions are listed in the following table.
Table 5-66 Troubleshooting errors with the drive board
Error code
Error name
Recommended
Error feature
action
into
the
valve-pump
data
Valve
output
failed
of limit (greater
than
40
or
equal to 0)
Communication
abnormal. Please try
again.
1. X motor error, action
X motor failed
0201
to
move
to
target position
is interfered.
2.
position
motor
target
photocoupler
error.
X motor failed
to
0202
move
home
to
position
after
initialization
position
after
initialization
0204
position
motor
initial
photocoupler
to leave home
Adjusting
2.
error.
X motor failed
0203
is interfered.
is interfered.
2.
position
motor
initial
photocoupler
error.
X
motor to target
position failed
is executed.
5-81
position
motor
target
photocoupler
Hardware System
error.
Adjusting
0205
motor back to
home
position
failed
X
motor to leave
home
position
failed
X
0207
motor
adjustment
X
motor
adjusting steps
out of limit
X
0209
is interfered.
2.
is executed
position
motor
initial
photocoupler
is interfered.
2.
is executed
position
motor
initial
photocoupler
error.
error
0208
error.
Adjusting
0206
9]
adjusting command
again
motor
end
adjusting
position error
Y motor failed
0211
to
move
to
target position
is interfered.
2.
position
motor
target
photocoupler
error.
1. Y motor error, action
Y motor failed
0212
to initialize to
upper position
is interfered.
2.
position
motor
upper
photocoupler
error.
1. Y motor error, action
Y motor failed
0213
to initialize to
lower position
lower
is interfered.
2.
position
motor
lower
photocoupler
error.
1. Y motor error, action
Y
is interfered.
motor to target
2.
position failed
is executed.
position
Adjusting
0214
error.
5-82
motor
target
photocoupler
Hardware System
1. Y motor error, action
Y
is interfered.
motor to upper
2.
position failed
is executed
position
Adjusting
0215
motor
upper
photocoupler
error.
Adjusting
0216
motor to leave
upper
position
failed
Y
0217
motor
error
0218
motor
adjusting steps
out of limit
Y
0219
adjusting
0220
is interfered.
2.
is executed
position
again
probe
is
adjust
sample probe
0226
sample
finished.
the
the
sample
probe
upward/downward/leftward/rightward
by one step before sending the
The action time is longer than the
overtime
download time.
X motor action
forbidden
match
current position
0225
the
X motor action
not
adjusting command
sample
probe
action is finished
X motor does
0224
Send
adjusting command.
0223
photocoupler
adjusting command
0222
upper
6]
end
assembly
Cannot
motor
working
0221
motor
position error
Sample
error.
adjustment
Y
probe
the
only
adjusting
command is sent.
upper
position
photocoupler
Y motor action
overtime
download time.
X motor action
forbidden
position
the
5-83
photocoupler
Hardware System
position
Y motor does
0227
not
match
current position
Y motor failed
0228
to
pierce
to
lower position
Y motor failed
0229
to
pierce
to
upper position
X
0230
motor
photocoupler
error
Sample
0231
RBC
Pierce again
Pierce again
Replace
damaged
photocoupler
Replace
damaged
photocoupler
Replace
damaged
photocoupler
Replace
damaged
photocoupler
Replace
damaged
photocoupler
motor
probe
bath
photocoupler
RBC
bath
error
Sample
0232
WBC
probe
bath
photocoupler
WBC
bath
error
Sample
0233
DIFF
probe
bath
photocoupler
DIFF
bath
error
Y
0234
motor
photocoupler
error
Sample
0235
motor
probe
upper
Upper
photocoupler
photocoupler
damaged
might
be
Replace
the
upper
photocoupler
error
Sample
0236
probe
lower
Lower
photocoupler
damaged
photocoupler
might
be
Replace
the
lower
photocoupler
error
Sampling syringe does not finish the
Sampling
0300
syringe
working
is
the
sampling
Hardware System
1.
Sampling
syringe
syringe
photocoupler
after action.
abnormal
sampling
syringe
motor moves)
2.
Sampling
motor
syringe
error
sampling
(if
syringe
Sampling
syringe
0302
Sampling
syringe
aspirating
or
dispensing
detected
action failed 1
disagrees
by
the
photocoupler
Sampling
motor
error
sampling
syringe
(if
the
syringe
Sampling
syringe
0303
Sampling
syringe
aspirating
or
dispensing
detected
action failed 2
disagrees
by
the
photocoupler
the
sample
syringe
Sampling
motor
error
sampling
syringe
(if
the
syringe
aspirating
or
dispensing
forbidden
Sampling
syringe
0305
dispensing
action
forbidden 2
Sampling
home
position
action
aspirating
or
position,
but
detected
syringe
the
actual
by
the
photocoupler disagrees
position)
Sampling
position
detected
photocoupler disagrees
5-85
by
the
home position)
syringe
Hardware System
Check and make sure
nothing is wrong with
Sample syringe
0306
aspirate volume
over range
volume
aspirated
aspirating
and
Sample syringe
0307
dispense
aspirating
Requested
volume
over
volume
>
aspirated
and
volume
range
syringe
action
overtime
0310
Sample
injection
while
aspirating or dispensing.
working.
syringe
is
working
1.
Sample
syringe
error
Sample
0311
injection
syringe
photocoupler
after action.
the
sample
injection
photocoupler
(if
sample
Sample
injection
abnormal
sample
injection
injection
0312
syringe
aspirating
dispensing
or
5-86
1.
Sample
syringe
error
injection
photocoupler
(if
sample
injection syringe is at
the home position)
Hardware System
action failed 1
2.
Sample
injection
injection
Sample
syringe
Sample
0313
error
injection
photocoupler
(if
sample
injection
syringe
2.
aspirating
or
dispensing
action failed 2
Sample
sample
injection
injection
syringe
0314
aspirating
or
dispensing
action
Sample
injection
syringe
photocoupler
error
(if
sample
injection syringe is at
the home position)
forbidden 1
Sample
injection
syringe
0315
aspirating
or
dispensing
action
Sample
injection
syringe
photocoupler
error
(if
sample
forbidden 2
Check and make sure
nothing is wrong with
Sample
injection
0316
syringe aspirate
volume
over
Requested
volume
aspirated
aspirating
and
range
Sample
0317
injection
syringe
Requested
volume
volume
>
aspirated
5-87
Hardware System
dispense
aspirating
volume
over
and
range
consider programming
overflow at the fluidics
debugging phase and
note the volume add
up error.
Sample
0318
injection
syringe
download time.
overtime
0320
sheath
fluid
syringe
is
working
Sheath
0321
fluid
syringe
photocoupler
after action.
abnormal
sheath
fluid
syringe
motor moves)
2. Sheath fluid syringe
motor error (if sheath
fluid
syringe
motor
fluid
syringe
0322
aspirating
or
dispensing
action failed 1
sheath
fluid
syringe
position)
at
the
home
position)
1. Sheath fluid syringe
Sheath
fluid
syringe
0323
aspirating
or
dispensing
action failed 2
fluid
syringe
5-88
Hardware System
fluid syringe motor is
at the home position)
Sheath
fluid
Before aspirating or dispensing, the
Sheath
dispensing
action
syringe
0324
aspirating
or
fluid
syringe
forbidden 1
Sheath
fluid
Before aspirating or dispensing, the
syringe
0325
aspirating
or
dispensing
action
Sheath
fluid
syringe
forbidden 2
fluid
syringe aspirate
Requested
volume
over
volume
aspirated
range
aspirating
and
Sheath
syringe
0327
aspirating
Requested
dispense
volume
fluid
over
volume
>
aspirated
volume
and
range
0328
Sheath
fluid
syringe
action
overtime
0330
Lyse syringe is
working
0331
Lyse
syringe
Lyse
syringe
Hardware System
photocoupler
abnormal
after action.
(if
the
lyse
Lyse
syringe
dispensing
action failed 1
error
Lyse
0332
syringe
aspirating
or
(if
the
lyse
Lyse
syringe
syringe
aspirating
or
dispensing
action failed 2
(if
the
lyse
syringe
aspirating
0334
or
dispensing
action
forbidden 1
Lyse
syringe
aspirating
0335
or
dispensing
action
forbidden 2
Lyse
Lyse
syringe
syringe
Lyse
0336
syringe
aspirate volume
over range
volume
aspirated
and
Hardware System
overflow at the fluidics
debugging phase.
Check and make sure
nothing is wrong with
the sampling syringe
Lyse
0337
syringe
dispense
volume
aspirating
Requested
over
volume
>
aspirated
volume
and
range
0338
Lyse
syringe
action overtime
0340
Diluent syringe
is working
the
diluent
Diluent
syringe
0341
Diluent syringe
photocoupler
abnormal
after action.
the
diluent
syringe
motor moves)
2.
Diluent
motor
syringe
error
(if
the
Diluent
syringe
0342
diluent
syringe
Diluent syringe
aspirating
position)
dispensing
2.
action failed 1
motor
or
Diluent
error
syringe
(if
the
at
the
home
position)
0343
Diluent syringe
1.
aspirating
dispensing
the
action failed 2
or
5-91
Diluent
diluent
syringe
syringe
Hardware System
home position)
2.
Diluent
motor
syringe
error
(if
the
or
dispensing
forbidden 1
Diluent syringe
0345
action
aspirating
or
dispensing
forbidden 2
syringe
diluent
syringe
action
Diluent
Diluent
syringe
diluent
syringe
Diluent syringe
0346
aspirate volume
over range
volume
aspirated
aspirating
and
Diluent syringe
0347
dispense
volume
aspirating
Requested
over
volume
>
aspirated
volume
and
range
0348
Diluent syringe
action overtime
download time.
Automatic
0390
testing
automatic
command
Reaction
command
is
received.
forbidden
0401
testing
bath
First
start
the
automatic
testing
Re-set
the
reaction
Hardware System
0402
of
the
MCU
pre-set
range
of limit
[30,50]
Optical system
of limit
[30,50]
bath temperature
Re-set
the
optical
system temperature
1. Check whether the
heater of the reaction
0403
Reaction
bath
temp. error
0404
is
well
Optical system
connected.
temp. error
Reaction
0405
bath
temp.
Compensation
compensation
value
and
ones
Optical system
0406
Compensation
value
and
compensation
ones
0407
compensation
error
Reaction
0408
bath
temp.
compensation
out of limit
Optical system
0409
temp.
compensation
out of limit
Compensation
value
and
bath
reaction
temp.
Reset
the
system
optical
temp.
compensation
error
Ambient temp.
the
compensation
error
temp.
Reset
ones
Reset
the
ambient
temp. compensation
Reset
bath
compensation
Reset
system
compensation
5-93
the
reaction
temp.
the
optical
temp.
Hardware System
Ambient temp.
040a
compensation
out of limit
Reset
temp. compensation
the
ambient
Ambient temp.
abnormal
range
temperature
transducer
and
measurement
the
circuit
work normally.
1. Check whether the
pneumatic connection
is well connected and
whether the pressure
Controlling
0601
pressure
chamber
pressure
failed
vacuum
chamber
vacuum failed
Adjusting
0603
pressure
chamber
pressure failed
0604
Adjusting
adjusting
again
adjusting command
previous
command
after
the
pressure
controlling is done.
The vacuum in the chamber is being
5-94
Hardware System
vacuum
controlled.
chamber
adjusting command
Can
not
send
the
vacuum failed
adjusting
again
command
after
previous
the
vacuum
controlling is done.
1. Check whether the
pneumatic connection
is well connected and
whether the pressure
Pressure
0605
chamber
pressure out of
limit
Vacuum
0606
chamber
vacuum out of
limit
Pressure
chamber
0607
pressure
control
range
Re-send
the
control
command of positive
range
error
Vacuum
0608
chamber
vacuum control
range error
FPGA
0701
download mark
error
0702
FPGA
Re-send
the
control
command of negative
range
Re-download
Re-download
Hardware System
download
defined.
command error
Read
0703
all
configuration
chip
content
finished
Data write out
of limit
The
head-address
over range
0706
Write forbidden
0707
Write overtime
0704
0705
data
written
plus
Re-download
the
Re-download
Send
the
enabling
command first
Re-download
1. Check the EEPROM
circuit
for
any
missing-soldered
component; check the
EEPROM pin for short
0731
Write-in
EEPROM error
etc.
2. If short circuit is not
found
and
components
properly
soldered,
all
are
it
in the control
code error
0802
0803
0804
0805
Data
length
error
again.
Communication
again.
error
Command label
Check
Communication
error
calculated one
Communication
abnormal. Please try
again.
Communication
abnormal. Please try
again.
Communication
Hardware System
in the communication rules. (016)
Identity code in
0806
0817
pack
analysis
error
to
analyze
Communication
abnormal. Please try
again.
the
Re-send
the
correct
command
If a certain pressure value is abnormal, check the related pumps, valves and airway
for leakage.
If the pneumatic system is normal, then the error should be caused by the damaged
pressure sensor on the drive board. Please replace the sensor or the drive board.
The pressure signals are outputted by the volumetric board. See the following table
for the voltage and pressure.
Table 5-67 Voltage signals and the corresponding pressures
Pressure
Pressure
channel
range
Pressure
chamber
2.54kPa
Test point
Relation
between
sensor
Note
P
TP30
represents
pressure, and
its unit is kPa.
Vacuum
-8
chamber
-40kPa
Volt
represents the
voltage of the
test point, and
its unit is V
Check whether the error-related mechanisms (such as motors) can move. If the
mechanisms do not move, then the error should be caused by the abnormal executive
components, such as disconnected or damaged motor. If nothing is wrong with them, then
5-97
Hardware System
the error should be caused by the damaged drive board, so the driver board should be
replaced.
If the mechanisms can move, check whether they move in position and whether they are
jammed by lines or other mechanisms during the movement. For transmissive
photocoupler, if the baffle enters above 2/3 photocoupler, it is baffled and moves is in
position.
If nothing is wrong with the mechanism action, consider the photocoupler error. Replace
the photocoupler.
If errors still remain after the photocoupler is replaced, then it should be caused by the
error components on the driver board. Replace the driver board.
5-98
Hardware System
Electromagnet control
Scanner control
Parameter storage
Block diagram
EEPROM
Clock
Scanner
Solenoid
UART1
C8051F020
FPGA
Configuration
Device
Reset
FPGA Reset
Power
Board
D 5V
P 12V
P 24V
Digital 5V
LDO
3.3V
3.3V
LDO
1.5V
SMXM
SMZM
SMRM
Sample Feed
SFXM
SFYM
Sensor
Status
Config
Config
Reset
IRQ
Sample Mix
1
Step Motor Control
BUS
EP1C3T14
4
Interface
JTAG UART0
Mother Board
RS422
Clock
Sensor
Description
The autoloader board provide the drive control to the following five stepping motors:
X-co motor of the mix mechanism
Z-co motor of the mix mechanism
Mix motor
5-99
Hardware System
X-co loading motor
Y-co feeding motor
The stepping motor drive is a type of bipolar consist-current half-step drive, and there are
position sensors corresponding to the stepping motors to detect their positions.
Electromagnet control
The electromagnet is used to control the process of opening the sample compartment door
(manual close).
The built-in scanner control is used to read barcodes on tubes and tube racks. The autoloader
board controls the built-in scanner through MCU, and the UART communication between MCU
and the built-in scanner through Serial port 1.
There are 13 position sensor and 1 sensitive switch on the autoloader. The status of the
sensors and switch is detected by FPGA, and MCU will check the status value of the FPGA
internal storage when necessary.
Parameter storage
As there may be manufacture or installation errors, it is necessary to adjust the initial positions
for the X-co motor of the mix mechanism and the mix motor. The adjusted position parameters
will be stored on the autoloader board.
Interface definition
The autoloader is configured with 14 external interfaces, shown as follows.
Table 5-68 External ports on the autoloader board
Interface
J1
J2
Number of pins
Function
FPGA
configuration
10
6
Description
FPGA
downloading
FPGA
debugging
J3
Electromagnet interface
J4
Reserved
Not applied to
J5
Power interface
the unit
J6
J7
Mix
mechanism
sensor
interface
Feeding
unit
sensor
interface 1
5-100
20
20
Hardware System
Feeding
J8
unit
sensor
20
interface 2
J10
Reserved
MCU
J11
configuration
/
/
10
interface
J12
Communication interface
J14
/
8
J16
P1
15
Definition of J1:
Name
Pin
F_DCLK
F_CONFDONE
F_CONFIG
F_DATA
F_ASDO
2
4
6
8
10
Name
AGND
VDD
F_NCE
F_NCSC
GND
Definition of J3:
Pin
Name
Description
12V0
solenoid
Mark
on
Wire
SOLENOID
Level
Note
12V
Current:1A
Definition of J5:
Table 5-71 Definition of the power interface
Pin
1
2
3
4
5
6
Name
D+5V
P+12V
P+24V
GND
PGND
PGND
Description
5V power supply (digital)
12V power supply (power)
24V power supply (power)
Digital ground
Power ground
Power ground
5-101
Hardware System
Definition of J6:
Name
V_X_START
X_START
3
4
5
GND
GND
V_X_END
X_END
7
8
9
GND
GND
V_Z_START
10
Z_START
11
12
13
GND
GND
V_Z_END
14
Z_END
15
16
17
GND
GND
V_R_START
18
R_START
19
20
21
GND
GND
V_SYRINGE
22
SYRINGE
23
24
GND
GND
Description
Sending end power signal of
X-co initial position
Photocoupler signal of X-co
initial position
Ground
Ground
Sending end power signal of
X-co end position
Photocoupler signal of X-co
end position
Ground
Ground
Sending end power signal of
Z-co initial position
Photocoupler signal of Z-co
initial position
Ground
Ground
Sending end power signal of
Z-co end position
Photocoupler signal of Z-co
end position
Ground
Ground
Sending end power signal of
mix mechanism initial position
Photocoupler signal of mix
mechanism initial position
Definition of J7:
5-102
Mark on Wire
Note
MX_START
Transmissive
sensor
MX_END
Transmissive
sensor
MZ_START
Transmissive
sensor
MZ_END
Transmissive
sensor
MR_START
Transmissive
sensor
Preserved
Transmissive
sensor
Hardware System
Table 5-73 Definition of the feeding sensor interface 1
Pin
Name
Description
V_X_START
3
5
GND
X_START
V_ X_END
4
6
GND
X_END
V_Y_START
9
11
GND
Y_START
V_Y_RIGHT
10
12
GND
Y_RIGHT
13
V_Y_LEFT
15
17
GND
Y_LEFT
14
V_UL_START
16
18
GND
UL_START
19
20
GND
X_READY
Definition of J8:
Mark
on
Wire
FX_START
Note
Transmissive
sensor
FX_END
Transmissive
sensor
FY_START
Transmissive
sensor
FY_RIGHT
Transmissive
sensor
FY_LEFT
Transmissive
sensor
F_UL_START
Transmissive
sensor
FX_READY
Sensitive
switch
Hardware System
Table 5-74 Definition of the feeding sensor interface 2
Pin
Name
Description
V_Y_READY
3
5
GND
VCC
Y_READY
V_UL_FULL
4
6
GND
VCC
UL_FULL
V_TUBE
11
GND
13
VCC
15
10
TUBE
VT_RACK
12
14
GND
VCC
16
17
RACK
VC_DOOR
18
DOOR
19
20
GND
NC
Mark
Wire
Definition of J11:
5-104
on
Note
Reserved
Reflective
photocoupler
F_UL_FULL
Reflective
photocoupler
F_TUBE
Reflective
photocoupler
Reserved
Reflective
photocoupler
DOOR
Reflective
photocoupler
Hardware System
Table 5-75 Definition of the MCU configuration interface
Pin
1
2
3
4
5
6
7
8
9
10
Name
VDD
GND
GND
TCK
TMS
TDO
TDI
NC
GND
NC
Description
Power signal
Ground
Ground
JTAG clock
JTAG mode selection signal
JTAG output data signal
JTAG input data signal
Floating
Ground
Floating
Definition of J12:
Name
RX+
RXTX+
TX-
Description
Serial data receiving end
Serial data receiving end
Serial data receiving end
Serial data receiving end
Definition of J14:
Name
Description
X_BN
X_B
X_AN
X_A
Y_BN
Y_B
Y_AN
Y_A
Mark on Wire
5-105
SFXM
SFYM
Hardware System
Definition of J15:
J15 is the control interface of X-co motor and Z-co motor for the mix mechanism.
Table 5-78 Definition of the interface of X-co motor and Z-co motor for the mix
mechanism
Pin
Name
Description
Mark on Wire
X_BN
X_B
X_AN
X_A
Z_BN
Z_B
Z_AN
Z_A
X-co
SMXM
X-co
X-co
X-co
Z-co
Z-co
SMZM
Z-co
Z-co
Definition of J16:
Pin
Name
Description
R_BN
Control
motor
Control
motor
Control
motor
Control
motor
R_B
R_AN
R_A
Mark on Wire
Definition of P1:
5-106
Hardware System
Table 5-80 Definition of the built-in scanner interface
Pin
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Name
VCC
TXD
RXD
GND
NC
CTS
OUTPUT1
\
Trigger
RTS
OUTPUT3
\
Chassis
OUTPUT2
NC
Description
Power signal
Serial data receiving end
Serial data sending end
Signal ground
Floating
Sending ready signal
Scanner out put signal 1
\
Trigger signal
Sending request signal
Scanner out put signal 3
\
Shielding ground
Scanner out put signal 2
Floating
5-107
Hardware System
Assembly drawing
5-108
Hardware System
Description
12V power supply status indicator. It turns on when the 12V power supply is
normal
5V power supply status indicator. It turns on when the 5V power supply is
normal
3.3V power supply status indicator. It turns on when the 3.3V power supply is
normal
MCU working status indicator. It flashes every 0.5s when MCU works
normally
FPGA working status indicator. It flashes every 0.5s when FPGA works
normally
Name
Pin
Note
TP1
PIN3 of X1
TP2
PIN47 of U2
RD signals of MCU
TP3
PIN62 of U2
WR signals of MCU
TP4
PIN10 of U2
TP5
PIN28 of U2
TP6
PIN5 of J1
TP7
VCC
+5VDC voltage50.25V
TP8
12V0
TP9
24V0
+24VDC voltage241.2V
10
TP10
VDD
+3.3VDC voltage3.30.15V
11
TP11
GND
12
TP12
GND
13
TP13
1V5
+1.5VDC voltage1.50.05V
14
TP15
TUBE
15
TP18
PIN3 of X2
5-109
Hardware System
16
TP21
PIN12 of U16
17
TP23
PIN12 of U18
18
TP24
PIN12 of U22
19
TP25
PIN12 of U23
20
TP26
PIN12 of U24
Key definition
The reset switch S1 on the drive board is used to reset the drive board manually. Press the
reset switch to reset the MCU and FPGA.
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
Disassembly
1. Shutdown the analyzer and cut off the power supply;
2. Open the left door;
3. Use the 107 cross-headed screwdriver to remove the two M4X8 panhead screws fixing the
shielding box, and then remove the box;
4. Unplug all the cords and connectors connected to the autoloader control board;
5. Use the 107 cross-headed screwdriver to remove the five M3X6 cross-headed panhead
screws fixing the autoloader control board, and then remove the board.
5-110
Hardware System
Assembly
Assemble the autoloader control board as per the above-mentioned steps in the reverse order.
Be sure to shut down the analyzer and cut off the power supply before
disassembling/assembling the board.
Verification
1. Check and make sure that all the components are properly connected to the autoloader
control board.
2. After ensuring the power supply is cut off, assemble the drive board and motherboard
properly, and then power on the analyzer.
3. Check if the indicators on the autoloader control board are in the following status: 1) D3,
D4 and D5 turned on; 2) D25 and D26 flashing. You can also check the indicators in the
self-test screen of the analyzer. If the self-test is passed, it means the autoloader control
board is replaced successfully; otherwise, you should troubleshoot the board.
5-111
Hardware System
5.4.5 Troubleshooting
Error analysis and solutions
Errors related to the autoloader board are identified and handled by the host control software
in the form of error codes. Note that only some of the error codes reported to the host control
software from the autoloader board can be handled automatically, while some need to be
judged with the help of users or service engineers. When an error is reported, the error No.
and error names are provided by host control software. The recommended solutions may
appear on the screen as the software help information.
Most errors of the autoloader board are identified by checking the photocoupler information, so
check the photocouplers first in case of errors. To check the photocouplers: first make sure that
the photocouplers are not blocked, and check if the status information of the photocouplers
from the software matches the actual status; hide the photocouplers with lighttight material,
and check if the status information matches the actual status. Inconsistency found in either one
of the two checks indicates invalidation of the photocouplers; otherwise, the error comes from
the board itself.
Typical errors and solutions:
Hardware System
according to the part related to mechanical structure of the analyzer in this manual, and make
sure the mechanism is out of error.
Error name
Error feature
Recommended action
1. Check if there are EEPROM elements not
welded, EEPROM pin short or mis-welded
2. If there are no such errors, then it is
identified as an EEPROM error and board
replacement is needed
5A00
EEPROM write
error
5201
SMXM action
failed
5202
SMXM action
failed
5203
SMXM action
failed
5204
SMXM action
failed
5205
SMXM action
failed
5206
SMXM action
failed
5207
SMXM action
failed
5208
SMXM action
failed
5209
SMXM
photocoupler
error
1. SMXM
home
position
photocoupler
error:
replace
the
photocoupler if it is confirmed to be a
photocoupler error
1.
1.
Hardware System
Current action forbidden
while the mix mechanism is
in motion
SMZM should not at the
home position, but the
home position photocoupler
is hide
SMZM should at the home
position, but the home
position photocoupler is not
blocked
5401
SMZM action
failed
5402
SMZM action
failed
5403
SMZM action
failed
5404
SMZM action
failed
5405
SMZM action
failed
5406
SMZM action
failed
5407
SMZM action
failed
5408
SMZM action
failed
5409
SMZM
photocoupler
error
5501
SMRM action
failed
5502
SMRM action
failed
5503
SMRM action
failed
5504
SMRM action
failed
5505
SMRM action
failed
5506
SMRM action
failed
SMRM action
failed
Mix mechanism
5508
5601
5-114
1.
1.
1.
Check
the
SMXM
and
SMRM
photocouplers,
and
replace
the
photocoupler when it is confirmed to be
an photocoupler error
Initialize the Z-co motor
1.
1.
1.
2.
Check
the
SMXM
and
SMZM
photocouplers,
and
replace
the
photocoupler when it is confirmed to be
an photocoupler error
Hardware System
action failed
Mix mechanism
action failed
Mix mechanism
action failed
current mechanism is in
motion
Number of mixing times out
of predefined range
Mix mechanism not
initialized
5606
Mix mechanism
action failed
5700
SFXM action
failed
5701
SFXM action
failed
5702
SFXM action
failed
5703
SFXM action
failed
5704
SFXM
photocoupler
error
5604
5605
5705
SFXM loading
error
5706
SFXM action
failed
5708
SFXM action
failed
5801
Functional
control code
error
5802
5803
Command label
error
5804
FX_READY sensitive
switch error: should be
switched off, but is detected
to be switched on
Functional code not defined
in the communication
protocol
Data length differs from that
of the current command
label
Command label not defined
in the communication
protocol
The check code differs from
the calculated check code
5-115
1.
Hardware System
5805
5806
5817
5-116
Hardware System
Block diagram
Description
The power board works with the 90V-264V AC input voltage (50-60Hz).
5-117
Hardware System
Once
the
AC
power
switch
is
turned
on,
all
the
circuits
work
and
the
Minimum
Nominal
Output voltage
Voltage
Load
current
current
range
adjustment
adjustment
rate
rate
Noise
D5V
2A
5A
4.85/5.25V
5%
100mV
+A12V
0mA
1A
11. 5/12. 5V
5%
100mV
-A12V
0mA
650mA
-11.5/-12.5V
5%
100mV
P12V
0.3A
5.5A
11.5/12.5V
5%
150mV
P24V
0A
4A
22/27V
10%
150mV
AC120V
0mA
60mA
115/145V(RMS)
10%
For the P24V, the 7.8A maximum current with the approximate lasting time of 2S and the
period of 60S may occur when working.
Interface definition
The power board is configured with 7 external interfaces. The J1 and J2 interfaces are
connectors of the socket; the rest of the interfaces are connectors of the plug terminal leading
from the board edges. The No. of the PCB board is TP1-TP20.The location of the interfaces on
the power board is shown below.
5-118
Hardware System
Function
AC input
Number of pins
Number of lands
Note
of the fans
PCB
Power supply output
A-J12
soldering
6
output
PCB
soldering
A-J13
AC output
5-119
output
Hardware System
PCB
soldering
10
10
output
PCB
soldering
output
Definition of J1:
Table 5-86 Definition of AC input interface
Pin
Definition
L, Live wire
N, Neutral wire
Definition of J2:
Table 5-87 Definition of the fan interface
Pin
Definition
P12V,connects the positive end of the P12V output
voltage
1/3/5
output voltage
Definition of AJ12:
Table 5-88 Definition of analog board output
Land
Definition
Pin
voltage
6,7
TP18A-12V
NC
8
1/2/3/4
Definition of AJ13:
5-120
Hardware System
Table 5-89 Definition of AC output
Land
Definition
Two
output
TP19\TP20
AC120
NC
ends
Pin
of
1/3
2
Definition of AJ35:
Table 5-90 Definition of power supply output
Land
Definition
positive end of the P12V
TP1P12V/TP2P12V
Pin
output voltage
TP12PGND/TP13PGND/TP3P
GND\TP4PGND\TP14PGND
output voltage
TP9P24V\TP10P24V\TP11P24
output voltage
4/5
6/7/8/9/10
1/2/3
Definition of AJ37:
Table 5-91 Definition of D5V output
Land
TP5D5V/TP6D5V
TP7DGND/TP8DGND
Definition
Pin
1/2
3/4
5V output voltage
Assembly drawing
5-121
Hardware System
5-122
Hardware System
Table 5-92 Test points on the power board
No.
Pin
Function
Reference
value
Q101.2
Q101.1
C114.+
39020V
U101.9
7.5V
U101.14
C206.+
VCC voltage
17V~22.5V
C204
VDD voltage
121V
C223
121V
Q201.1
10
Q201.2
11
U201.8
5V
12
U201.4
13
Q201.3
waveform
14
C232.+
50.5V
15
C311.+
121V
16
U301.4
17
U301.8
8V
18
Q303.1
19
Q303.2
20
Q303.3
waveform
21
C326.+
12VB voltage
120.5V
22
C328.+
130V DC output
115V150V
23
C332.+
A+12V output
120.5V
24
C338.-
A-12V output
-120.5V
25
U351.15
120.5V
26
U351.5
27
U351.11
28
U351.14
29
U351.16
5V
30
Q361.1
31
Q362.1
32
Q363.1
33
Q364.1
34
C409.
121V
5-123
Hardware System
35
U401.5
36
U401.14
5V
37
U401.9
38
Q406.1
39
Q406.2
40
C423.+
22~27V
41
C442.+
120.5V
Purpose
Damaged power assembly or power board needs to be replaced by a new one.
Tools
107 cross-headed screwdriver
Sharp-nose pliers
Multimeter
Disassembly
Disassembly of the power board consists of the following two main procedures (removing the
whole power assembly and further disassembly of the inner components):
5. Unplug the 3 cables connecting the power assembly from the mother board; loose the
cable fixer and remove the cables.
6. Use the 107 cross-headed screwdriver to remove the cross-headed panhead screws
(M4X8) fixing the earth wire from the ground pole.
7. Use the 107 cross-headed screwdriver to remove the 6 cross-headed panhead screws
(M4X8) fixing the power assembly.
8. Draw out the power assembly for 50-100mm and unplug the 2 cable connectors
connecting the power switch.
5-124
Hardware System
9. Draw out and remove the power assembly.
1. Use the 107 cross-headed screwdriver to remove the 2 cross-headed panhead screws
(M4X8) fixing the power cover, and then remove the cover together with the power socket
from the power assembly.
2. Use the 107 cross-headed screwdriver to remove the 2 cross-headed panhead screws
(M3X8) fixing the power socket, and then disassembly the power socket.
3. Use the 107 cross-headed screwdriver to remove the 4 cross-headed panhead screws
(M3X8) fixing the shielding box, and then remove the shielding cover.
4. Use the shape-nose pliers to loose the 6 M3 nuts fixing the fan and unplug the cable plug
from the power board, and then remove the fan.
5. Use the 107 cross-headed screwdriver to remove the 9 cross-headed panhead screws
(M3X6) from the power board, and then remove the power board.
5-125
Hardware System
4 ---Fan
5---M3 Nut
6---Power board
10 ---Power cover
Assembly
Assemble the power board as per the above-mentioned steps in the reverse order.
Be sure to shut down the analyzer and cut off the power supply before
disassembling/assembling the board.
Verification
1. Check and make sure the earth wire of the AC input socket is properly connected to the
conductive cover of the main unit.
2. Connect the power cord and turn on the AC switch, then the fan works.
5-126
Hardware System
When assembling, before fixing the upper cover of the power assembly, be
sure the AC plug and the plug of the fan are connected with the power
board.
Be sure the screws are tightened to firmly fix the power assembly with the
main unit.
5.5.5 Troubleshooting
5-127
Hardware System
Be sure the screws are tightened to firmly fix the power assembly with the main unit.
5-128
Hardware System
Provides the time signals of RBC/PLT and WBC count, i.e. the start and end time of
running.
Provides the vacuum and pressure intensity signals of the vacuum chamber and pressure
chamber, sends the signals to the drive board.
Block diagram
Figure 5-27 Block diagram of the volumetric and pressure detecting board
5-129
Hardware System
Description
The WBC count start/end signals detected by the WBC volumetric sensor are I-V transferred,
level-transformed and de-bounced by the volumetric board, and then sent to the drive board.
The RBC/PLT count start/end signals detected by the RBC/PLT volumetric sensor are I-V
transferred, level-transformed and de-bounced by the volumetric board, and then sent to the
drive board.
The working status of the volumetric sensor is controlled by the drive board through J1
connector. When the level of the control signal is high, the volumetric sensor doesn't work;
when the level is low, the volumetric sensor works.
The vacuum/pressure intensity signals of the vacuum/pressure chamber are transferred to the
voltage signals by the vacuum/pressure sensor on the volumetric board. Then, they are further
transferred to the A/D-required voltage signals and then sent to the drive board through the J1
connector.
Interface definition
The volumetric board has only one 12PIN connector connecting the drive board. The location
of the connector is shown below.
5-130
Hardware System
Pin
Signal description
Signal Type
P+12V
PGND
Power ground
DGND
Digital ground
POSI_PRESS
Analog
NEGA_PRESS
Analog
DGND
Digital ground
#VM_CTRL
TTL level
RBC_START
TTL level
RBC_STOP
TTL level
10
WBC_START
TTL level
11
WBC_STOP
TTL level
12
D+5V
5-131
Hardware System
Assembly drawing
Hardware System
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
Sharp-nosed pliers
Disassembly
1. Shut down the analyzer;
2. Open the right door;
3. Use the 107 cross-headed screwdriver to remove the 2 cross-headed panhead screws
(M3X8) fixing the shielding box, and then remove the shielding cover.
4. Disconnect the 4 pipes connecting the volumetric tube.
5. Use the 107 cross-headed screwdriver to remove the 2 cross-headed panhead screws
(M3X8), and use the sharp-nosed pliers to remove the 2 socket cap screws (M3X16) fixing
the volumetric board.
6. Pull out the volumetric board for 30-50mm; unplug the cable connectors on its back and
disconnect the 2 pipes, and then remove the board.
3---Volumetric tube
4---Rubber ring
7---Volumetric board
5-133
Hardware System
Assembly
Assemble the power board as per the above-mentioned steps in the reverse order.
Verification
1.
2.
3.
Running samples in the whole blood mode. If the RBC/WBC clogging, bubbles and
abnormal pressure/vacuum are not reported by the analyzer, the replacement is
succeeded. If one or more above-mentioned error(s) is reported, you should remove the
error accordingly.
5.6.4 Troubleshooting
Table 5-94 Troubleshooting the volumetric board
No.
Error
Error name
type
Error feature
Causes
Probability
Improper
with
power
supply error
System
error
The voltage of
caused
broken cable
point
Improper
is
equal
not
to
12V0.6V.
by
supply
main
Reconnect the
Medium
cable
or
change a new
cable
power
of
the
unit
or
Improper
power
sent
action
cable
connection
+12V
Recommended
+12V
See
troubleshooting
Low
supply
by
of
the
board
the
drive
and
power board
mother board
The voltage of
2
+5V
power
supply error
Board
error
is
equal
5V0.25V.
not
to
L2 is damaged
(or rosin jointed)
or something is
wrong
(rosin
5-134
Low
Replace
or
re-solder
the
components
Hardware System
Improper
cable
connection
with
by
Reconnect the
Medium
cable
or
change a new
cable
broken cable
Something
is
reference power
supply wrong
Board
error
The
TP6
output voltage
2.5V0.25V
+5V
power
Low
Something
is
Low
Improper
with
error
error
Voltage of the
VM_CTRL
signal 0.8V
caused
by
Medium
Control
signals
through
Control
signal
error
Control
volumetric
sensor error
the
Board
error
change a new
Low
troubleshooting
of
the
drive
board
Medium
circuit
error (Q1 or U2 is
Standard
voltage (TP5)
2.5V0.25V
or
See
is wrong
of
cable
cable
source
the
broken cable
board
re-solder
Reconnect the
Constant current
or
cable
connection
System
Replace
U8
rosin jointed.
signal
table
supply.
U8 chip or it is
Control
wrong
or
rosin
jointed; resistors
R10, R11, R18,
R23,
R25
and
R24 is wrong or
rosin jointed)
5-135
Medium
Replace
or
re-solder
the
components
Hardware System
Adjust VWST,
VWSP,
VRST
and VRSP to
VWST,
VWSP,
VRST or VRSP
are not adjusted
make
Medium
well.
the
voltage of the
point WT, WP,
RT
and
RP
equals
Voltage
6
of
the
2.7V0.1V
Voltage of the
Board
error
RT and RP
expected value
2.7V0.1V
to
respectively.
Volumetric
sensor U15, U21,
U16 or U17 is
Low
Replace
or
re-solder
the
components
jointed).
Constant current
source
of
the
volumetric
Medium
sensor error
+5V
power
supply error
Low
Indicators D1,
D2, D3 and D4
are off when
7
Indicator
Board
abnormal
error
fluid flows or
are
still
on
when no fluid
flows
through
Low
rosin jointed)
+5V
supply error
the
Low
is
change
or
component
power
Something
table
Re-solder
D1, D2, D3 or D4
is damaged (or
standard
Low
power supply
the volumetric
Voltage
tube
of
RP
the
Medium
2.7V0.1V
8
Constant current
Board
Standard
+12V
source error of
error
voltage TP10
supply error
5-136
power
Low
Hardware System
the
vacuum
2.5V0.25V
sensor
R47
or
U4
is
damaged
(or
Re-solder
Medium
or
change
the
component
rosin jointed).
+12V
Constant current
9
source error of
Board
the
error
pressure
sensor
power
supply error
Standard
voltage TP7
2.5V0.25V
Low
or
U10
is
damaged
(or
Medium
or
change
the
component
rosin jointed).
+12V
Pressure/vacuum
10
unit
+2.5V
reference power
Board
error
supply error
power
supply error
Standard
voltage TP9
2.5V0.25V
Low
Medium
change
Adjust
is
not
adjusted well.
Pressure/vacuum
11
1.25V
voltage
error
The
the
component
VR9
or
VR9,
making
Medium
the
relative voltage
of the TP9 to
TP8
relative
1.2500.002V
Board
voltage of the
error
Pressure/vacuum
1.2500.002V
2.5V
power
reference
supply
Medium
See No.10 in
the table
error
U6 is damaged
or rosin jointed
12
Pressure
output
voltage abnormal
Board
+12V
error
pressure
is
supply error
0HPa
or
Constant current
the
source error of
450HPa,
output voltage
the
of the point PP
sensor
is not equal to
1.25V
1.2500.002V
error
5-137
power
pressure
voltage
Medium
Replace
or
re-solder
the
components
Low
Medium
Medium
Hardware System
or
Adjust PPZ to
2.2500.002V.
make
the
voltage of the
PP
is
1.2500.002V
when the input
PPZ or PPG is
not adjusted well
pressure is 0.
Medium
Then,
adjust
PPG to make
the voltage of
the
PP
is
1.2500.002V
when the input
pressure
is
450HPa.
U12, U14, R34,
R44, R45, R46,
R50, R52, C10 or
Re-solder
Medium
C23 is damaged
change
or
the
component
Vacuum
output
voltage abnormal
Board
+12V
error
pressure
is
supply error
0HPa
or
Constant current
the
source error of
-450HPa,
output voltage
the
of the point NP
sensor
is not equal to
1.25V
1.2500.002V
error
5-138
power
vacuum
voltage
Low
Medium
Medium
Hardware System
or
Adjust NPZ to
0.2500.002V.
make
the
voltage of the
NP
is
1.2500.002V
when the input
NPZ or NPG is
not adjusted well
pressure is 0.
Medium
Then,
adjust
NPG to make
the voltage of
the
NP
is
0.2500.002V
when the input
pressure
is
-450HPa.
U12, U13, R33,
R40, R41, R42,
R43, R51, C7 or
C22 is damaged
(or rosin jointed)
5-139
Re-solder
Medium
change
component
or
the
Hardware System
transferring,
level-transforming
and
de-bouncing.
Finally,
the
Block diagram
LEO
I
tube
LEO(I) Sensor
I-V
transform
Level transform
LEO
II
tube
LEO(II) Sensor
I-V
transform
Level transform
J1
LH tube
LH Sensor
I-V
transform
Level transform
CL tube
CL Sensor
I-V
transform
Level transform
5-140
Mother
Board
Hardware System
Description
For the liquid level of the LEO(I), LEO(II), LH and cleanser are detected by the same principle,
so the liquid level detection of LEO(I) is used just to be the example.
The status (sufficient/insufficient) signals of the LEO(I) detected by the sensor is conducted the
I-V converting, level-transforming and de-bouncing by the liquid-level detecting board and then
sent to the drive board.
The working status of the detecting sensors is controlled by the drive board through J1
connector. When the level of the control signal is high, the detecting sensors do not work;
when the level is low, the detecting sensors work.
Interface definition
The liquid level detecting board has only one connector of 8PIN connecting the drive board.
The location of the connector is shown below.
J1
5-141
Hardware System
Table 5-95 Pin definition of the liquid-level detecting board
No.
Pin
Signal description
Signal Type
D+5V
LIQ1
TTL level
LIQ2
TTL level
LIQ3
TTL level
LIQ4
TTL level
#D_CTRL
TTL level
DGND
Digital ground
Assembly drawing
5-142
Hardware System
Purpose
Damaged liquid-level detecting board needs to be replaced by a new one to ensure the
operation of the analyzer. Please follow the procedures to have the board replaced.
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
Disassembly
Liquid-level detecting board disassembly consists of the following two main procedures:
1.
2.
3.
Use the 107 cross-headed screwdriver to remove the 2 cross-headed panhead screws
(M4X8) fixing the shielding box, and then remove the shielding cover.
4.
Pull the 4 pipes out from the pipe clamp of the liquid-level detecting board.
5.
Use the 107 cross-headed screwdriver to remove the 4 cross-headed panhead screws
(M3X6) from the liquid-level detecting board.
6.
Pull out the liquid-level detecting board for 30-50mm, unplug the cable connectors on its
back, and then remove the board.
5-143
Hardware System
panhead
screw
2---Liquid-level
detecting
board
cover
shielding
1.
Use the 107 cross-headed screwdriver to remove the 4 cross-headed panhead screws
M3X8 fixing the pipe clamp.
2.
3---Pipe clamp
5-144
Hardware System
Assembly
Assemble the power board as per the above-mentioned steps in the reverse order.
Verification
1.
2.
3.
Start up the analyzer properly. Running samples in the whole blood mode. If No LEO(I)
(/LEO(II)/LH /cleanser) or bubbles are not reported by the analyzer, the replacement is
succeeded. If one or more above-mentioned error(s) is reported, you should remove the
error accordingly.
5.7.4 Troubleshooting
Table 5-96 Troubleshooting the liquid-level detecting board
No.
Error name
Error
Error
type
features
Causes
Probability
Improper
with
caused
action
cable
connection
or
Recommended
by
Reconnect
Medium
the
cable or change a
new cable
broken cable
The voltage
1
+5V power
System
supply error
error
of the +5V
test point is
not equal to
5V0.25V.
Improper
power
by
See
Low
troubleshooting of
the
drive
board
the
mother board
L2 is damaged (or
rosin jointed) or
something
wrong
is
(rosin
Low
Replace
or
re-solder
the
components
reference
power
Board
error
The
TP1
output
voltage
Something
wrong
with
is
the
Low
Hardware System
supply
2.5V0.25V
wrong
Something
wrong
is
with
the
chip U9 or R10 or
they
are
Low
rosin
Replace
or
re-solder the U9
jointed.
Improper
cable
connection
with
Control
System
signal error
error
Voltage
of
or
the
caused
disconnection
by
Reconnect
Medium
the
cable or change a
new cable
broken cable
D_CTRL
signal
Control
signals
See
0.8V
Low
troubleshooting of
the drive board
mother board is
wrong
Control
signal
error
Abnormal
driving
4
of
the
detecting
Board
error
sensor
Voltage
to
Control
ground
of
circuit
collector
U3
electrode of
wrong
Q1 is not
jointed;
between
4.4V-5.25V.
or
Medium
U4
or
are
rosin
resistors
Medium
Replace
or
re-solder
the
components
The voltage
of
voltage
point
point VLL1,
VLL1,
5
of
Board
VVL2,
error
VVL3
and
VVL4 is not
VVL4
the
2.2V0.1V
VVL2,
VVL3
and
VVL2,
Medium
expected
value.
Detecting sensors
U1, U2, U3 or U4
are
wrong
rosin jointed).
5-146
(or
Low
Replace
or
re-solder
the
components
Hardware System
Abnormal driving
of the detecting
Medium
sensor
+5V power supply
error
LED1,
Low
LED2,
Indicator
Board
abnormal
error
Indicators
error
LED1,
Something
LED2,
wrong
with
and
TP1
LED4
are
power supply
the
standard
still
on
voltage of point
when
the
VLL1,
is
filled
with
Low
change
or
the
component
Low
is
LED3
tube
table
Re-solder
jointed)
+5V power supply
VVL2,
Low
Medium
2.2V0.1V
fluid or are
U7,
off when no
fluid exists
in the tube.
R5,
R15,
R11,
R19,
R30,
R23,
R25,
5-147
Re-solder
Medium
change
component
or
the
Hardware System
Power conditioning
Block diagram
Description
Power conditioning
The power conditioning module filters the 12V power sent by the data board. After filtering,
the ripple is less than 100mV.
The working current of the laser is measured and sent to the data board for monitoring.
The laser control board controls the laser by constant-power method. I.e. the laser output
5-148
Hardware System
power is monitored by the inner photoelectrical detector, and the results obtained forms a
closed loop system through negative feedback to ensure the constant-power output. The
power is controlled (3mW-5mW) by adjusting the potentiometer VR1 on the board.
Interface definition
The laser control board is configured with 2 external connectors. One is the J1 connecting the
mother board (connecting data board through the mother board), the other one is the J2
connecting the laser, as the figure shows.
Definition
AVSS
Note
-12V
Direction I/O
Analog
power
Level
-12V
No greater than 5V
supply
2
AGND
Analog ground
LASER
Monitoring
voltage
of
AVCC
12V
#CONTROL
I/O
by standard voltage
VCC
5-149
5V
Hardware System
Table 5-98 Definition of J2 (connecting the laser)
No.
Name
Note
Direction
Level
I/O
1
LDA
LDC
PDA
Assembly drawing
5-150
Hardware System
Never try to plug or unplug the semi-conductor laser before the power
supply is cut off.
See the test points in the table below and their locations on the board in the assembly drawing.
Table 5-99Test points of the laser control board
No.
Test
Signal under
point
test
Function
A+12V
AVCC
A-12V
AVSS
D5V
VCC
Digital
4
AGND
ground
\
on
board
5
TPVREF
VREF
TP2VREF
2VREF
voltage
to
ground
of
the
rheostat
TPIPD
PDA
TPILD
LASER
5.8.5 Troubleshooting
The troubleshooting information of the laser control board is listed in the table below.
5-151
Hardware System
Table 5-100 Troubleshooting the laser control board
No.
Error
Error
Features
Recommended action
Possible Causes
Improperly connected
or disconnected with
+12V
power
supply error
The voltage of
board
point exceeds
the range of
12V0.6V
Something is wrong
with
board.
the
power
Grounding
short-circuits.
The
power is lower or
higher.
Something is wrong
-12V
power
supply error
The voltage of
with
point exceeds
board.
the range of
short-circuits.
-12V0.6V
power is lower or
the
power
Grounding
The
higher.
The voltage of
3
5V
power
supply error
Improperly connected
or disconnected with
power
is
sent
the
laser
is proper.
to
board.
The voltage of
4
Control signal
the
error
#CONTROL
0.8V
signal
Improperly connected
or disconnected with
control
board
are proper.
5-152
Hardware System
Reference
voltage error
The voltage of
TPVREF
power
U1
works
2.5V0.1V
(regards -12V
chip
as reference)
jointed or damaged.
U1
First
is
rosin
ensure
the
TPVREF is normal.
Then,
the
slide
is
rosin
Laser
The voltage of
rheostat
intensity
TP2VREF
jointed or damaged.
control
abnormal
reference
(should
voltage error
3.9V-4.1V)
is
Or,
be
something
wrong
components
is
with
the
soldering
of
the
operation
amplifier
U2
and
its
peripherals.
Laser
diode
damaged
or
cable,
connected improperly
or
Laser
The voltage of
5V voltage error
intensity
TPILD
monitoring
exceeds
or succeeding control
check
signals TPILD
range
circuit error
circuit.
output error
0.8-2V
the
of
The
operation
the
succeeding
5-153
Hardware System
Power conditioning
Optical-electrical converting
Signal conditioning
Block diagram
Description
Power conditioning
The power conditioning module filters the 12V power sent by the data board. After filtering,
the ripple is less than 50mV.
Optical-electrical converting
The optical-electrical converting module converts the optical signals into the electrical signals
by the photodiode.
5-154
Hardware System
Signal conditioning
The current signals are transferred into the voltage signals through I/V and then sent to the
succeeding amplifying-conditioning module for further processing. Then, the data board input
required signals are obtained.
Interface definition
The FS/SS pre-amplification board is configured with one external connector respectively, i.e.
the J1 connector connecting the mother board (connecting data board through the mother
board). The layout of the board is shown below.
Definition
Note
Direction
Level
I/O
1
AGND
Analog ground
FS/SS
SHELL
Shielding ground
AVSS
-12V5%
AGND
Analog ground
AVCC
12V5%
1V
The laser control board is configured with 2 external connectors. One is the J1 connecting the
5-155
Hardware System
mother board (connecting data board through the mother board), the other one is the J2
connecting the laser.
Definition
Note
Direction I/O
Level
AVSS
-12V
AGND
Analog ground
LASER
Monitoring
5V
voltage
of
AVCC
12V
#CONTRO
I/O
by standard voltage
High level: OC
door of 5V
VCC
5V
Name
Note
Direction I/O
Level
LDA
LDC
PDA
5-156
Hardware System
Assembly drawing
(a) Top
(b) Bottom
Figure 5-44 Assembly drawing of the FS/SS pre-amplification board
5-157
Hardware System
Test
Signal under
point
test
Digital
AGND
Function
\
ground
on
board
2
+12V
AVCC
-12V
AVSS
OUT
OUT
D5V
VCC
5.9.5 Troubleshooting
Table 5-105 Troubleshooting the pre-amplification board
No.
Error
Error Features
Possible Causes
Improper
test
cable
connection
12V
+12V
point
power
supply
120.6V;
board
error
ripple
or
the
noise
is
Recommended action
Rosin
joint
or
damage
5-158
Hardware System
Improper
cable
connection
-12V
-12V
point
power
supply
-120.6V;
board
error
ripple
test
or
the
noise
is
Rosin
joint
or
damage
C21.
SS
output
signals
output
value
error
component
too
strong
(supposing
it
by mistake.
output
is
by mistake.
signals
FS
Channel
circuit problems)
component
The
The photodiode D1 or R1 is
component
too
or damaged.
component
pre-amplification
board
(FF/SS)
weak
to
be
detected.
Something is wrong
with the band width
of
pre-amplification
board (FF/SS).
the
component
5-159
Hardware System
Introduction
The indicator board realizes the functions of analyzer status indicating and buzzer alarming.
5.10.2
Board Composition
Function
Buzzer alarming
Description
The indicator board indicates the working status of the analyzer by a two-color indicator. When
the system works normally, the indicator displays green. When error happens, the indicator
turns red.
Buzzer alarming
Interface definition
The indicator board is configured with one external connector J1.
Table 5-106 Interface definition of the indicator board
PIN
Name
Note
Direction
Level
I/O
VCC
Power supply
+5V
FAULT
TTL
WORK
TTL
BUZ
Buzzer
TTL
GND
Ground
Working
indicator
(green)
5-160
Hardware System
Assembly drawing
5.10.3
Purpose
Damaged indicator board needs to be replaced by a new one.
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
Disassembly
1. Shut down the analyzer.
2. Open the front cover assembly.
3. Use the 107 cross-headed screwdriver to remove the 2 tapping screws (PT3X6) fixing the
indicator board, and then remove the board.
5-161
Hardware System
Figure 5-46 Disassemble the indicator board and the key board
1---Key board
3---Front cover
Assembly
Assemble the indicator board as per the above-mentioned steps in the reverse order.
Verification
Turn on the analyzer and start up the PC when finish replacing the indicator board. During the
process from startup self-test to ready, closely observe the indicator and the buzzer, if they
work normally, the replacement is successful.
5.10.4
Troubleshooting
Table 5-107 Troubleshooting the indicator board
No.
1
Error
Indicator doesn't work
system alarming.
5-162
Hardware System
Introduction
The key board enables the operator to interact with the main unit through the [RUN] and
[OPEN] keys during the autoloading/manual loading process.
5.11.2
Board Composition
Function
[RUN] key
[OPEN] key
Description
The operator can press the [OPEN] key to open the sample compartment door.
Interface definition
The indicator board is configured with one external connector J1.
Table 5-108 Interface definition of the key board
PIN
Name
Note
Direction
Level
I/O
COUNT_KEY
[RUN] key
TTL
INSERT_KEY
[OPEN] key
TTL
GND
Ground
5-163
Hardware System
Assembly drawing
5.11.3
Purpose
Damaged key board needs to be replaced by a new one.
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
Disassembly
1. Shut down the analyzer.
2. Open the front cover assembly.
3. Use the 107 cross-headed screwdriver to remove the 4 tapping screws (PT3X8) fixing the
key board, and then remove the board.
5-164
Hardware System
Figure 5-48 Disassemble the key board and the indicator board
1---Key board
3---Front cover
Assembly
Assemble the key board as per the above-mentioned steps in the reverse order.
Verification
Turn on the analyzer and start up the PC when finish replacing the key board. Wait for the PC
interface to show ready, and then press the [OPEN] key in the front cover. If the sample
compartment door opens, then the key board is successfully replaced.
5.11.4
Troubleshooting
As the structure of the key board is quite simple, it seldom has errors. If there is no response
after pressing the keys, connect the 2 and 4 pins, as well as the 3 and 4 pins of the J1 interface.
If the circuit is closed after pressing one of the keys, the key board is out of error and you
should check other components for errors; if the circuit is open, the key is not working properly.
5-165
Maintenance
Type
Settings to be reset
Re-adjust the position of the sampling assembly
by software
1
2
Maintenance
4
5
6
Remove the sample compartment
assembly from the analyzer and
then re-assemble it
7
8
9
10
11
12
13
14
6-1
Maintenance
Replace
disassemble/assemble
bottom cover
and
the
15
Replace the aperture
16
17
18
19
20
21
Sequence
Replace HGB
The vacuum created in the
sequence is changed
Sample results are changed
greatly
6-2
Maintenance
6.2 General
You can service the analyzer as per Section 6.3 6.7 . After repairing, follow the verification
procedures to verify the analyzers status.
6-3
Maintenance
Tools
107 flat-headed screwdriver
Removal
1. Power off the analyzer and unplug the power cord
2. Insert 107 the flat-headed screwdriver into the slot of the door lock, and turn the lock
tongue 90 counterclockwise.
3. Pull the side door outward from the bottom.
Installation
Install the left door as per the above-mentioned steps in the reverse order.
6-4
Maintenance
Tools
107 flat-headed screwdriver
Removal
1. Power off the analyzer and unplug the power cord.
2. Insert 107 flat-headed screwdriver into the slot of the door lock, and turn the lock tongue
90 counterclockwise.
3. Pull the side door outward from the bottom.
Make sure that the power supply is shut off when you remove the right door;
otherwise, the analyzer will alarm and stop running.
6-5
Maintenance
Installation
Install the right door as per the above-mentioned procedures in the reverse order.
Tools
107 cross-headed screwdriver
Removal
1. Power off the analyzer and unplug the power cord.
2. Use the 107 cross-headed screwdriver to remove the three M4X8 small panhead screws.
3. Pull out the top cover from back upward.
6-6
Maintenance
2 top cover
Installation
Install the top cover as per the above-mentioned procedures in the reverse order.
Tools
107 cross-headed screwdriver
6-7
Maintenance
Removal
1. Power off the analyzer and unplug the power cord.
2. Use the 107 cross-headed screwdriver to remove the two M4X8 small panhead screws
used for fixing the back panel of power supply
3. Pull the back panel outward.
Installation
Install the back panel as per the above-mentioned procedures in the reverse order.
Tools
107 cross-headed screwdriver
6-8
Maintenance
107 flat-headed screwdriver
Removal
1. Power off the analyzer and unplug the power cord.
2. Remove the right door as per section 6.3.3 .
3. Turn the handspike handle 90 (clockwise and counterclockwise) to make it slide into the
slot in the front board, and the front door will be pushed open by the handspike.
4. Lift the front door upwards.
2 Handspike
3 Back panel
4 Mix mechanism
5 Front panel
6 Front door
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
6-9
Maintenance
Removal
1. Power off the analyzer and unplug the power cord.
2. Remove the left door as per section 6.3.1 .
3. Remove the right door as per section 6.3.2 .
4. Remove the top cover board as per section 6.3.3 .
5. Make sure there are no tubes or tube racks left at the sample transport area (if there are,
remove them).
6. Use the 107 cross-headed screwdriver to remove the six M4X8 countersunk head screws
on the left, right and top side of the front cover.
7. Pull the front cover outward by 50 - 80 mm, and disconnect the indicator connector from
the indicator board.
8. Remove the front cover forward.
2 top cover
3 Key board
5 right door
8 left door
Installation
Install the front cover as per the above-mentioned procedures in the reverse order.
6-10
Maintenance
3 plain washer 3
5 diluent syringe
6 lyse syringe
8 pressure chamber
9 air pump
10valve assembly II
6-11
Maintenance
4 vacuum chamber
7 pinch valve
6-12
Maintenance
2 pressure-leakage valve
4 sheath syringe
5 injection syringe
6 sampling syringe
7 plain washer 3
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
Removal
Follow these two procedures to disassemble the valve assemblies.
To remove the valve assembly (valve assembly I, valve assembly II, or valve assembly III)
6-13
Maintenance
1. To remove valve assembly I and II, remove the left door (as per section 6.3.1 ); to remove
valve assembly III, remove the right door (as per section 6.3.2 ).
2. Disconnect all tubes connected to the valve(s) as shown in Figure 6-7, Figure 6-8, and
Figure 6-9.
3. Use the 107 cross-headed screwdriver to remove the M4X12 small panhead screws
fastening the valve assembly.
4. Pull the valve assembly, outward and disconnect all lines from the assembly..
5. Take out valve assembly.
To remove a single valve (including pinch valve, or single valve on the valve assemblies)
1. To remove the valve on the left side, remove the left door (as per section 6.3.1 ); to remove
the valve on the right side, remove the right door (as per section 6.3.2 ); to remove the
valve on the front side, remove the front cover assembly. (as per section 6.3.2 )
2. Disconnect all pipes connected to the valves as shown in Figure 6-7, Figure 6-8, and
Figure 6-9 (if a pinch valve needs to be removed, remember to pinch the corresponding
tube).
3. Use the 107 cross-headed screwdriver to remove the two M3X8 small panhead screws
fastening the valve assembly.
4. Pull the valve outward, and disconnect the lines connected to the valve. Then take the
valve out.
6-14
Maintenance
3 valve bracket
Installation
Install the valve assembly as per the above-mentioned procedures in the reverse order.
Verification
1. Check the fluidic connections.
2. Power on and verify the installation.
Maintenance
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
Removal
Follow these two procedures to disassemble the pressure chamber.
1. Use the 107 cross-headed screwdriver to remove the four M3X12 panhead washer screws,
then take out the end cap and mounting plate;
2. Use the 107 flat-headed screwdriver to remove the airproof screw fixed on the end cap,
then take out the small O ring (9.5x1.8).
3. Take out the big O ring (48.7x1.8).
2 end cap
5 airproof screw
6 mounting plate
6-16
Maintenance
Installation
Install the pressure chamber as per the above-mentioned procedures in the reverse order.
Verification
1. Check if all parts are correctly assembled and tightened.
2. Check the fluidic connections..
3. Power on and verify the installation.
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
Removal
Follow these two procedures to disassemble the vacuum chamber.
1. Use the 107 cross-headed screwdriver to remove the four M3X12 panhead washer screws,
then take out the end cap and mounting plate.
2. Use the 107 flat-headed screwdriver to remove the air tightening screw fixed on the end
cap, then take out the small O ring (9.5x1.8).
3. Take out the big O ring (48.7x1.8).
6-17
Maintenance
cross-headed
panhead
2 end cap
4 O ring (9.5x1.8)
5 airproof screw
6 mounting plate
Installation
Install the vacuum chamber as per the above-mentioned procedures in the reverse order.
Verification
1. Check if all parts are correctly assembled and tightened.
2. Check the reagent connections.
3. Power on and verify the installation.
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
2 mm hexagon wrench
6-18
Maintenance
2.5 mm hexagon wrench
150 mm monkey wrench
Removal
Follow these two procedures to disassemble the syringe.
1. To the remove lyse syringe or diluent syringe, remove the left door (as per section 6.3.1 );
to the remove sheath syringe, injection syringe or sampling syringe, remove the front
cover assembly (as per section 6.3.5 ).
2. Disconnect all tubes connected to the syringe as shown in Figure 6-7 and Figure 6-9.
3. Use the 107 cross-headed screwdriver to remove the four M3X12 panhead washer screws
fixing syringe and 3 plain washer. .
4. Incline the assembly outward, and pull it out by 30 - 50mm. Disconnect the wire of the
motor and the photocoupler, and then take the assembly out. (* Note that the four rubber
washers of the syringe should be kept well for reassembly.)
a. To remove the glass syringe unit on the lyse syringe (three 2.5ml syringes on the
assembly), remove the left door (as per section 6.3.1 ).
b. To remove the glass syringe unit on the injection syringe or on the sample syringe (250ul
and 100ul respectively), remove the front cover (as per section 6.3.5 or 6.3.6 ).
c. The syringe assembly needs not to be disassembled wholly. Use the 107 flat-headed
screwdriver to remove the M4X8 special screw fixing the syringe. Rotate the left glass
syringe unit with hand, and then take out the washer of syringe (as per Figure 6-15).
Apply some glue to the threads. Do not proceed to the next step until the
glue is dry.
6-19
Maintenance
3 washer
d. Use the 107 cross-headed screwdriver to remove the two M3X8 socket head screws on
the electric switch and remove the switch. Use the 107 cross-headed screwdriver to
remove the two M3X6 cross-headed panhead screws, and then remove the electric switch
(Figure 6-16).
6-20
Maintenance
Linear
2 syringe slider
rolling guide
3 M3X8 socket head screw
screw
7 syringe photocoupler bracket
e. Use the 107 cross-headed screwdriver to remove the syringe protection shield. Use
2.5mm hexagon wrench to remove the four M3X8 socket head screws on syringe slider.
Use 2 mm hexagon wrench to remove M4X8 retaining screw on syringe slider and remove
the syringe slider. Use 2.5 mm hexagon wrench to remove the four M3X8 socket head
screws fastening the linear stepping motor , then remove the linear stepping motor (Figure
6-21
Maintenance
6-16);
Make sure to insert the retaining screw into the corresponding hole, and
apply some glue to the threads.
f. Use 2.5mm hexagon wrench to remove the three M3X8 socket head screws on linear
rolling guide, and then remove the linear rolling guide. (*Note: The slider should not be off
its guide way.)
2. Remove diluent syringe and sheath syringe (*Note: Two syringes are the same, both being
the 10ml Mindray syringe.)
a. To remove the Mindray syringe unit on the diluent syringe, remove the left door (as per
section 6.3.1 )
b. To remove the Mindray syringe unit on the sheath syringe, remove the front cover
assembly (as per section 6.3.5 or 6.3.6 )
c. The syringe assembly should not be removed wholly. Use the 107 cross-headed
screwdriver and flat-headed screwdriver to remove the 10ml syringe presser the two
tailor-made M3X12 socket head screws, and remove the Mindray syringe unit. (Figure
6-17)
6-22
Maintenance
d. Use the 107 cross-headed screwdriver to remove the four M3X10 panhead screws. The
ceramic plug, flange, lock ring and O ring can be disassembled in the axis direction.
(Figure 6-18)
PT3X10
cross-headed
panhead
screw
3 10ml syringe flange
5 O ring 15X3
Before replacing the lock ring, make sure relative parts (especially the
operation surface of the ceramic plug) are clean, and then insert the plug
into the flange, lock ring and O ring in turn and make it fixed to the syringe
body.
Installation
Install the Mindray syringe unit as per the above-mentioned procedures in the reverse order.
Verification
1. Check if all parts are correctly assembled and tightened.
2. Check wire connections.
3. Check reagent connections.
4. Power on and verify the installation.
6-23
Maintenance
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
100 mm monkey wrench
Removal
Follow these two procedures to disassemble the waste pump.
1. Power on the analyzer to empty the fluidic system. Power off the analyzer and unplug the
power cord from the wall outlet.
2. Remove the right door (as per section 6.3.2 ).
3. Disconnect the four tubes connected to the pump;
4. Disconnect the two wires connected to the pump;
5. Use the 107 cross-headed screwdriver to remove the two M4X12 small panhead screws
on fastening assembly as shown in Figure 6-8.
6. Move the assembly left, make the fastening hole out of the bolt, and then remove it.
1. Use the 107 cross-headed screwdriver to remove the four M3X8 small panhead screws
fixing the pump.
2. Remove the waste pump
3. Use the 100 mm monkey wrench to remove the three nuts and take out the three washers.
4. Remove the pump mounting plate.
5. Remove three shock pads via hand.
6-24
Maintenance
2 rotary pump
4 8-32UNC-2B nut
5 GB93 4 washer
6 shock pad
7 bracket
Installation
Install the waste pump as per the above-mentioned procedures in the reverse order.
Verification
1. Check if all parts are correctly assembled and tightened.
2. Check the electrical connection of the rotatory pump.
3. Check the reagent connections.
4. Power on and verify the installation.
6-25
Maintenance
Tools
107 cross-headed screwdriver
107 flat-headed screwdriver
Removal
1. Power on the analyzer to empty the fluidic system. Power off the analyzer and unplug the
power cord from the wall outlet.
2. Remove the left door (as per section 6.3.1 ).
3. Remove the right door (as per section 6.3.1 ).
4. Remove the top cover (as per section 6.3.1 ).
5. Use the 107 cross-headed screwdriver to remove the two M3X6 cross-headed washer
screws on the top cover of data board shielding box, and then remove the top cover.
6. Disconnect the bath signal lines of the WBC reaction bath or RBC reaction bath that needs
to be removed, and then loosen all buckles fastening the lines in the direction of wiring,
until the wire is drawn to the bath.
7. Use the 107 cross-headed screwdriver to remove M3X8 small panhead screw on bath
shield and take it out, then remove the shield. (Figure 6-21)
8. Use the 107 cross-headed screwdriver to remove the two M3X8 small panhead screws on
bath presser and take it out, then remove the bath presser. (Figure 6-21)
9. Pull the bath out with a distance of about 50-80 mm. Disconnect all tubes connected to the
bath. Get the signal wire from wire-protective ring and then remove the WBC bath
assembly or the RBC bath assembly.
6-26
Maintenance
washer screw
3 data board
2 WBC bath
3 bath presser
5 RBC bath
6 shield cover
8 isolation chamber
6-27
Maintenance
Installation
Install the bath assembly as per the above-mentioned procedures in the reverse order.
Verification
1. Check if all parts are mounted to the former position and are fastened.
2. Check if the connection of signal lines of WBC or RBC is correct or not.
3. Check if the connection of power line and reagent pipe is correct or not.
4. Power on and verify the installation.
6-28
Maintenance
Tools
Probe wipe
open vial sample probe / piercing needle
metal wipe clip
sample probe presser
Preparation
1. Power off the analyzer if it is running.
2. Turn off the main power switch on the right side of the analyzer and unplug the power cord.
3. Remove the right door (as per section 6.3.2 ).
4. Move the sampling module to the back of analyzer.
Removal
1. Remove the probe wipe from the sampling module (Figure 6-22)
6-29
Maintenance
3 probe wipe
4 wipe clip
2. Remove the sample probe from the sampling module. (Figure 6-23)
a. Pull out the sample aspiration tube from the above of sample probe.
b. Use the cross-headed screwdriver to remove the retaining screw (M3X8 small
panhead screw) on probe presser, and take out the fixing flake.
6-30
Maintenance
2 sample probe
3 presser
When remove the sample probe, get probe wipe out of the presser under
guiding rod. The probe tip may contain biohazardous materials. Exercise
caution when handling it and keep it to the right position.
Installation
To install a new sample probe and wipe, repeat the above removal step 1 or 2 in the reverse
order.
6-31
Maintenance
Tools
Preparation
1. Power off the analyzer and unplug the power cord.
2. Turn off the main power switch on the right side of the analyzer and unplug the power cord.
3. Remove the right door (as per section 6.3.2 ).
Removal
1. Remove the vertical optical sensor module (Figure 6-24)
a. Disconnect the connector of the sensor to be replaced ( not shown in the figure).
b. Use the cross-headed screwdriver to remove the M3x6 screw on the sensor.
c. Remove the sensor module from the bracket.
3 bracket
2. Remove the horizontal optical sensor module (Figure 6-25)
a. Use the cross-headed screwdriver to remove the M3x6 screw on the photocoupler
6-32
Maintenance
presser to be replaced
b. Gently pull out the photocoupler presser (with the sensor module on motor position)
from the sample mother board and get out the plug of sensor module from the
corresponding hole.
c. Disconnect the sensor connector (not shown in figure). Take out the photocoupler
presser and the sensor module on motor position.
d. Use the cross-headed screwdriver to remove the M3x6 screw fixing the sensor module.
Separate the photocoupler presser from the sensor module on the motor position.
Installation
To install a new optical sensor, repeat the above removal step 1 or 2 in the reverse order.
Tools
wire cutter
Sampling module
6-33
Maintenance
Preparation
1. Power off the analyzer.
2. Turn off the main power switch on the right side of the analyzer and unplug the power cord.
3. Move the analyzer to turn its right side outward, and expose the back side as much as
possible.
4. Open the back cover of the analyzer (as per section 6.3.2 )
5. Remove the right side door (as per section 6.3.2 )
6. Move the sampling module to the back of the analyzer.
Removal
1. Disconnect all tubes from on the sampling module, and cut the binding belt with the wire
cutter. Remove the tubes from the whole sampling module.
2. Disconnect all the connectors of the vertical part of sampling module, and unplug the
connector from the motor directly.
3. Disconnect all the connectors of the horizontal part of sampling module from the space left
by opened back cover at the back of the analyzer. (BC-5380 has four connectors, and
BC-5380 has five connectors). Unplug the connectors from the motor directly.
4. Use the cross-headed screwdriver to remove the two M3X8 small panhead screws fixing
the joint.
5. Use the cross-headed screwdriver to remove the four M4X12 cross panhead screws fixing
the sampling module.
6. Hold the sampling module and move it gently outward. Pull out the optical sensor module
from corresponding hole with caution. Be careful not to damage or break the line.
7. Check if all connectors of optical sensor module are drawn out from the corresponding
holes, then hold out the sampling module.
6-34
Maintenance
2 link
3 spacer
4 sampling module
6-35
Maintenance
Installation
To replace new sampling module, repeat the above step 1 to 7 in the reverse order.
6-36
Maintenance
Tools
wire cutter
Preparation
1. Power off the analyzer.
2. Turn off the main power switch on the right side of the analyzer and unplug the power cord.
3. Remove the right side door (as per section 6.3.2 )
Removal
1. Use the wire cutter to cut off the binding belts fixing the tubes and remove them from the
DIFF bath assembly.
2. Use the cross-headed screwdriver to remove the two M4X8 small panhead screws fixing
the DIFF bath assembly.
3. Hold the DIFF bath assembly and move it gently outward. Expose the connectors and pipe
connectors at the back side, and then disconnect them.
4. Take out the DIFF bath assembly.
6-37
Maintenance
Installation
To replace new DIFF bath assembly, repeat the above removal step 1 to 4 in the reverse order.
Remember to readjust the position of the sample probe over the DIFF bath as per Step 11 to
14 in Section 6.10.1 .
6-38
Maintenance
and
replacing
the
closed-tube
sample
compartment assembly
Purpose
To remove or replace the closed-tube sample compartment, follow steps in this section.
Tools
Damp gear
PHOTOELEC switch
Gear
Electromagnet
Preparation
1. Power off the analyzer.
2. Turn off the main power switch on the right side of the analyzer and unplug the power cord.
3. Remove the right side door (as per Section 6.3.2 )
4. Remove the left side door (as per Section 6.3.1 )
5. Remove the top cover (as per Section 6.3.3 )
Removal
Follow these two procedures to disassemble the waste pump.
6-39
Maintenance
1. Use the 107 cross-headed screwdriver to remove the two M3X6 cross-headed panhead
screws, and then remove the electromagnet assembly; remove the two M3X6
cross-headed panhead screws in the electromagnet assembly, and then remove the
electromagnet body; remove the E ring to take out the spring, as shown in Figure 6-29
6-40
Maintenance
2 Electromagnet assembly
3 E ring
4 Spring
6 Electromagnet body
2. Use the 107 cross-headed screwdriver to remove the M4X12 cross-headed panhead
screw fixing the photocouplers, and then remove the photocouplers, as shown in Figure
6-30
6-41
Maintenance
3. Use the 1.5mm hexagonal wrench to remove the two M3X5 set screws, and then remove
the gear; use the 107 cross-headed screwdriver to remove the two M3X6 cross-headed
panhead screws fixing the damp gear, and then remove the damp gear, as shown in
Figure 6-31.
2 Gear
3 Damp gear
4. Use the sharp-nose pliers to remove the spring from either of the two fixing ends, as
shown in Figure 6-32.
5. Use the sharp-nose pliers or the small flat-headed screwdriver to remove either of the two
D2 E rings on the shaft, and loosen the two M3X5 set screws in the tube holder
compartment fixing the shaft. Then take out the shaft and remove the tube holder
compartment body and the helical torsion springs.
During installation, make sure the M3X5 set screws are fixed in the same
level of the notches in the shaft, and one of the screws should be plunged
into the cone-shaped hole.
6-42
Maintenance
2 Shaft
5 Pulling spring
6. Use the 1.5mm hexagonal wrench to remove the M3X5 set screw fixing the shaft, and then
you can take out the shaft, deep groove ball bearing, wheel, bush and gasket in turn, as
shown in Figure 6-33.
During installation, the two bearings should be put into the wheel (the
bearings are supposed to be cling to the inner of the wheel) until their ends
reaches those of the wheel; the set screws should be fixed in the level of the
notches in the shaft.
6-43
Maintenance
3 Wheel
4 Bush
5 Gasket
Installation
To replace the sample compartment assembly with a new one, repeat the first removal
procedure (step 1 to 4) in the reverse order. During installation, take into account the
following items:
1. One side of the sample compartment assembly should be set close to the positioning step
raised on the bottom board (autoloader bottom board)
2. Be sure to install the assembly back to the position marked in disassembly.
1. The tube holder compartment body should be put right in the center
2. Always install the damp gear after the gear is installed
3. Turn the core of the electromagnet 360 and make sure there is no interference
4. Move the guiding axis of the electromagnet with you hand and make sure the sample
compartment can turn properly. Push the compartment body back to the initial position and
make sure it can be locked up.
6-44
Maintenance
Tools
Sensitive switch
PHOTOCOUPLER (reflective)
Preparation
1. Power off the analyzer.
2. Turn off the main power switch on the right side of the analyzer and unplug the power cord.
3. Remove the right side door (as per Section 6.3.2 )
4. Remove the left side door (as per Section 6.3.1 )
5. Remove the top cover (as per Section 6.3.3 )
Removal
1. Remove the front cover assembly (as per Section 6.3.6 )
2. Unplug the cable connector corresponding to the switch to be replaced
3. Use the 101 cross-headed screwdriver to remove the two M2X8 cross-headed panhead
screws fixing the sensitive switch detecting the proper loading of tube racks, and then
remove the sensitive switch.
4. Use the 107 cross-headed screwdriver to remove the M2X8 small panhead screws fixing
the switches detecting the empty/full status of the unloader, and then remove the switch,
as shown in Figure 6-34.
6-45
Maintenance
2 PHOTOCOUPLER (reflective)
4 Sensitive switch
Installation
To replace the switch with a new one, repeat Step 1 to 4 above in the reverse order.
Tools
Maintenance
Preparation
1. Power off the analyzer.
2. Turn off the main power switch on the right side of the analyzer and unplug the power cord.
3. Remove the right side door (as per Section 6.3.2 )
4. Remove the left side door (as per Section 6.3.1 )
5. Remove the top cover (as per Section 6.3.3 )
Removal
Follow the two procedures to remove and disassemble the sample transport unit.
6-47
Maintenance
6-48
Maintenance
4 Riser unit
5 Unloader unit
6 Platform unit
8 Backboard
2. Unplug the connector of the stationary barcode scanner; use the 107 screwdriver to
unscrew the four M3X8 small panhead screws fixing the stationary barcode scanner and
its bracket on the backboard unit, and then remove the stationary barcode scanner and its
bracket, as shown in Figure 6-36 and Figure 6-37
3. Use the 107 cross-headed screwdriver to remove other units on the backboard like the
tube detecting photocouplers and the tube rack locating plate as per Figure 6-37
During installation, make sure the back of the tube locating block and that of
the tube rack locating plate are at the same plane; adjust the distance
between the tube rack block and the tube rack to be around 0.2mm.
6-49
Maintenance
3 Backboard bracket
10PHOTOCOUPLER (reflective)
6-50
Maintenance
5 Sensory plate
7 Gear shaft
8 Short rack
9 Gear holder
10Long rack
13Unloader bracket
6. Use the 107 cross-headed screwdriver to remove the two M3X8 small panhead screws
fixing the spring presser, and then remove the spring presser, as shown in Figure 6-36 and
Figure 6-39
6-51
Maintenance
3 Spring presser
5 Shaft sleeve
6 Sensory plate 3
7. Use the 107 cross-headed screwdriver to remove the two M3X8 small panhead screws
fixing the front supporting board, and then remove the front supporting board, as shown in
Figure 6-36 and Figure 6-40
8. Unplug the connectors of the sensitive switch and the PHOTOCOUPLER (reflective) on
the platform unit; use the 107 cross-headed screwdriver to remove the six M3X8 small
panhead screws fixing the platform unit, and then remove the platform unit, as shown in
Figure 6-36 and Figure 6-40
6-52
Maintenance
9. Unplug the connectors of the stepping motor and the three photoelectric sensor in the
left-and-right loading unit; use the 107 cross-headed screwdriver to remove the three
M3X8 small panhead screws fixing the left-and-right loading unit, and then remove the
left-and-right loading unit, as shown in Figure 6-36 and Figure 6-41
10. Use the 1.5mm hexagonal wrench, 2.5mm hexagonal wrench and the 107 cross-headed
screwdriver to remove the left-and-right loading unit as per Figure 6-41
6-53
Maintenance
3 Washer
6 E ring GB/T896-86.4
11Belt presser
14Shaft sleeve
15M3X12
16Pallet
cross-headed
panhead
19Synchronous belt
11. Unplug the connectors of the stepping motor and the two photoelectric sensor in the
forth-and-back loading unit; use the 107 cross-headed screwdriver to remove the four
M3X8 small panhead screws fixing the left-and-right loading unit, and then remove the
forth-and-back loading unit, as shown in Figure 6-36 and Figure 6-42
12. Use the 1.5mm hexagonal wrench, 2.5mm hexagonal wrench, 101 cross-headed
screwdriver and the 107 cross-headed screwdriver to remove the forth-and-back loading
unit as per Figure 6-42
6-54
Maintenance
2 Washer
8 Right pusher
10Belt presser
set screw
11M3X5 cheese head hexagon screw
12Pulling spring
14Shaft sleeve 2
screw
21M3X8 cheese head hexagon screw
6-55
Maintenance
Installation
Repeat the removal steps in the reverse order to install.
Take into account the following items during installation and adjustment:
Install the guiding rail close to one side, and put in the positioning screws before installation
Before installing the synchronous pulley of the driven belt shaft, apply some lubricator on the
surface of the shaft.
Make sure the shaft end of the stepping motor and the end of the synchronous pulley are at
the same plane.
While installing the stepping motor, adjust the tensility of the synchronous belt before locking
up screws.
Install the guiding rail close to one side, and put in the positioning screws before installation
Before installing the synchronous pulley of the driven belt shaft, apply some lubricator on the
surface of the shaft.
Make sure the shaft end of the stepping motor and the end of the synchronous pulley are at
the same plane.
While installing the stepping motor, adjust the tensility of the synchronous belt before locking
up screws.
Make sure the two racks can move freely with little resistance after installation. If the resistance
is large, repair is necessary; otherwise, the unit may not be able to restore the initial position.
See the initial positions of the short rack in Figure 6-43 with one tooth hanging over the end.
While installing the assembled sample transport unit to the main unit, readjust tbe sample
proble position as per Step 1 to 10 in Section 6.10.1
6-56
Maintenance
Verification
1. Readjust the position of the sample probe as per Step 10 to 14 in Section 6.10.1 .
2. Check if all parts are mounted to the former position and are fastened.
3. Check if the connection of signal lines of WBC or RBC is correct or not.
4.
6-57
Maintenance
Tools
Preparation
1. Power off the analyzer.
2. Turn off the main power switch on the right side of the analyzer and unplug the power cord.
3. Remove the right side door (as per Section 6.3.2 )
4. Remove the left side door (as per Section 6.3.1 )
5. Remove the top cover (as per Section 6.3.3 )
Removal
Follow the two procedures to remove and disassemble the mix mechanism.
1. Remove the shielding box on the autoloader board, and unplug the cable connectors
between the mix mechanism and the autoloader control board
2. Remove the front cover assembly (as per Section 6.3.6 )
3. Use the 107 cross-headed screwdriver to remove the four M4X8 panhead screws, and
then remove the mix mechanism, as shown in Figure 6-44.
6-58
Maintenance
1. Unfasten the plastic cable tie fixing the components and pull out the plugs from the five
photocouplers and three motors to the cable, and then remove the cable
2. Use the 107 cross-headed screwdriver to remove the three M3X6 cross-headed panhead
screws fixing the small motor protection cover, and then remove the cover, as shown in
Figure 6-45
3. Use the 2.5mm hexagonal wrench to remove the three M3X8 cheese head hexagon
screws connecting the rotation and X-co motion unit and the Z-co motion unit, and then
remove the two units, as shown in Figure 6-45
6-59
Maintenance
Maintenance
Figure 6-46
5. Use the 107 cross-headed screw driver to remove the four M3X6 cross-headed panhead
screws fixing the two photocouplers on the 43 motor installation board, and then remove
the motor installation board and the elements on the board (draw out the motor shaft from
the motor nut), as shown in Figure 6-46
6. Use the 107 cross-headed screw driver to remove the three M3X6 cross-headed panhead
screws fixing the motor nut, and then remove the nut, as shown in Figure 6-46
7. Use the 2.5mm hexagonal wrench to remove the four M3X5 cheese head hexagon screws
fixing the connection block, and then remove the block, as shown in Figure 6-46
8. Use the 107 cross-headed screw driver to remove the two M3X6 cross-headed panhead
screws fixing the Z-co sensory plate, and then remove the two Z-co photocoupler sensory
plates, as shown in Figure 6-46
9. Use the 2.5mm hexagonal wrench to remove the five M3X5 cheese head hexagon screws
fixing the linear guiding rail MGN9C1R175Z0CM, and then remove the guiding rail, as
shown in Figure 6-46
10. Use the 2.5mm hexagonal wrench to remove the two M3X6 cross-headed panhead
screws fixed in the slider connecting board for baffling
6-61
Maintenance
1 Protection cover
2 Photocouplers
4Connecting block
5 Motor nut
8 Bracket
11. Use the 107 cross-headed screw driver to remove the two M3X6 cross-headed panhead
screws fixing the sensor installation bracket, as shown in Figure 6-47
12. Use the 2.5mm hexagonal wrench to remove the four M3X8 cheese head hexagon screws
fixing the linear stepping motor E43H4Q, and then remove the linear stepping motor
E43H4Q, four M3 large washer and 8 O rings, as shown in Figure 6-47
2 O ring
6 M3 large washer
8 Sleeve
13. Use the 107 cross-headed screw driver to remove the six M3X6 cross-headed panhead
screws fixing the photocouplers, X-co sensory plate, X-co sensor installation board, and
then remove the photocouplers, X-co sensory plate, X-co sensor installation board, as
shown in Figure 6-48
14. Use the 107 cross-headed screw driver to remove the four M2.5X6 cross-headed panhead
screws fixing the linear stepping motor, and then remove the stepping motor E28H41, as
shown in Figure 6-48
15. Use the 2.5mm hexagonal wrench to remove the two M3X8 cheese head hexagon fixing
the 28 motor installation board, and then remove the installation board, as shown in Figure
6-48
6-62
Maintenance
16. Use the 2.5mm hexagonal wrench to remove the four M3X5 cheese head hexagon screw
fixing the connecting board, and then remove the connecting board
17. Use the 2.5mm hexagonal wrench to remove the four M3X5 cheese head hexagon screw
fixing the linear guiding rail MGN9C1R75Z0CM, and then remove the guiding rail
18. Use the 107 cross-headed screw driver to remove the two M3X6 cross-headed panhead
screws fixed in the slider connecting board for baffling
4M2.5X6
cross-headed
panhead
screw
5 Slider connecting board
linear
guiding
rail
MGN9C1R75Z0CM
7 Connecting board
11 Photocouplers
19. Use the 2.5mm hexagonal wrench to remove the M3X8 hexagon screw fixing the presser,
6-63
Maintenance
and then remove the presser, as shown in Figure 6-49
20. Use the 2.5mm hexagonal wrench to remove the M3X8 hexagon screw fixing the rotation
photocoupler plate, and then remove the rotation photocoupler plate, as shown in Figure
6-49
21. Use the 2.5mm hexagonal wrench to remove the M3X8 hexagon screw fixing the swing
arm and use the 2mm hexagonal wrench to remove the M4X8 set screw fixing the swing
arm, and then remove the swing arm
22. Use the 107 cross-headed screw driver to remove the two M3X6 cross-headed panhead
screws fixing the spring tube pincher, and them remove the M3 large washer and the
spring tube pincher, as shown in Figure 6-49
23. Use the 2.5mm hexagonal wrench to remove the four M3X10 cheese head hexagon
screws fixing the rotation stepping motor, and then remove the four M3 large washer, the
rotation stepping motor and the synchronous belt, as shown in Figure 6-49
24. Use the 1.5mm hexagonal wrench to remove the four set screws fixing the two small
pulleys, and then remove the pulleys, as shown in Figure 6-49
25. Use the 2.5mm hexagonal wrench to remove the two M3X8 screws fixing the bearing
holder, and then remove the bearing holder, pulley shaft and the two deep groove ball
bearings, as shown in Figure 6-49
26. Use the 107 cross-headed screw driver to remove the three M3X6 cross-headed panhead
screws fixing the linear stepping motor E28H41 nut, and then remove the linear stepping
motor E28H41 nut, as shown in Figure 6-49
27. Use the 107 cross-headed screw driver to remove the M3X6 cross-headed panhead screw
fixing the rotation sensor bracket, and then remove the rotation sensor bracket and the
photocouplers
6-64
Maintenance
1 Presser
3 Rubber washer
5 Swing arm
8 Small pulley
9 Pulley shaft
11
Rotation
sensor
bracket
and
12 Bearing holder
photocouplers
13 Deep groove ball bearing
18 M3 large washer
20 Synchronous belt
Installation
Perform the steps above in the reverse order to install.
During installation or position adjustment, take into consideration the following items:
1. Install the guiding rail close to one side, and put in the positioning screws before
installation
2. Make sure the motion parts can move properly
3. Keep the synchronous belt fully tightened
4. Adjust the initial position of the pincher (for forth-and-back movement, left-and-right
movement, and rotation) while installing the assembled unit to the main unit. See Section
6.10.2 for how to make adjustment.
6-65
Maintenance
6.10 Adjustment
6.10.1
1. Switch off the power of the analyzer and open the sample compartment manually. Put the
sample probe in-compartment locating fixture in the sample compartment and close the
compartment door, as shown in Figure 6-50. Loosen the three screws on the sample
transport unit shown in Figure 6-51, Figure 6-52 and Figure 6-53. Move the sample probe
assembly and sample transport unit horizontally and enable the sample probe to aim at the
central hole in the locating fixture, and then fix the three screws.
2. Connect the reagents properly and connect the main unit and the PC with a network cable.
Start up the PC software on the main unit and on the PC. Then, enter the user name
service and the password Se s700 (note: there is a space between e and s) into the
pop-up box, and then click Ok to initialize the analyzer. During initialization, a message
box will pop up asking you whether to Ignore fluidic initialization?. Click Yes to skip
the initialization.
3. Press the [OPEN] key on the front cover, and the sample compartment will be opened
automatically. Put the sample probe in-compartment locating fixture in the sample
compartment, as shown in Figure 6-54.
6-66
Maintenance
6-67
Maintenance
Click Start
6-68
Maintenance
Click Stop
Click Forward,
Backward,
Up and Down
Maintenance
transport assembly, as shown in Figure 6-59
Click Start
Maintenance
making the probe inserted into the slot of the locating fixture. Click Up and put a paper on
the AL positioning fixture, and then click Down until the sample probe reaches the paper,
as shown in Figure 6-61. Click Stop to save the autoloading position of the sample probe,
and then remove the sample probe AL positioning fixture, as shown in Figure 6-62.
Click Stop
Click Forward,
Backward,
Down and Up
Figure 6-61 Adjusting the sample probe AL piercing position using the software
Maintenance
bath, as shown in Figure 6-63.
Maintenance
Click Forward,
Backward, Up
and Down
Click Stop
Click Ok
Figure 6-65 Adjusting the sample probe DIFF bath up position using the software
14. Click Ok to exit the Debug screen.
6.10.2
1. Switch off the power of the analyzer and put the tube rack at the piercing position of the
sample transport unit, making the recess of the tube rack lap over the presser, as shown in
Figure 6-66, Figure 6-67 and Figure 6-68.
Recess
Figure 6-66 The recess of the tube rack
6-73
Maintenance
Presser
Four screws
Tube locating
fixture
Maintenance
password Se s700 (note: there is a space between e and s) into the pop-up box, and
then click Ok to initialize the analyzer. During initialization, a message box will pop up
asking you whether to skip the initialization. Click Yes to skip the initialization
4. Click Menu Service Debug to enter the Debug screen
5. Click Manipulator and Start in turn, and the pincher will move onto the tube grabbing
position, as shown in Figure 6-70.
Click Start
Maintenance
Backward, Up and Down to make the tube locating fixture able to insert in the hole of
the AL piercing position locating fixture. Click Up and remove the two fixtures, as shown
in Figure 6-72 and Figure 6-73.
Click
Left,
Right Forward,
Backward, Up
and Down
Click Stop
Click Ok
Figure 6-72 Adjusting the pincher position using the software
6-76
Optical System
Tools
Antistatic gloves
Hexagon wrench
Cross-headed screwdriver
Flat-headed screwdriver
Horologic driver
Duke (4K-07) 7m standard particles
Oscilloscope or oscillometer
7-1
Optical System
Screws
7-2
Optical System
7.2 Removing
and
Installing
Optical
System
Assemblies
The optical system consists of the following assemblies: the front light assembly, flow cell
assembly, rear light collimator assembly, beam splitter assembly, rear light collector assembly,
and rear light detector assembly.
Be sure to turn off the analyzer and disconnect the power cord before
removing boards.
7-3
Optical System
support
7-4
Optical System
7-5
Optical System
3. Use the hexagon wrench to remove the two connecting screws and divide the flow cell
assembly into two parts: rectifier assembly and detecting assembly, as Figure 7-5 shows.
4. Clean or replace the detecting assembly and liquid transporting assembly as needed.
3Detecting assembly
2O ring
4Hexagon screw
5. Install the flow cell assembly as stated above in the reverse order.
7-6
Optical System
4. Install the new rear light diaphragm lens assembly on the rear light guide assembly to form
a new rear light collimator assembly. Ensure that the superface edge of the diaphragm
lens assembly parallels the end face edge of backward oriented assembly. Install and
secure the assembly to the optical support.
Optical System
screwdriver to remove the screws at the top board and the side boards to remove the
boards.
4. Remove the two protective washers to remove the beam-splitting prism.
5. When replacing or installing a new beam-splitting prism, pay attention to the direction of
the prism (see Figure 7-7 ). Install the prism following steps above in the reverse order.
4Clamping ring
2Side board
5Washer
3Top board
6Beam-splitting prism
LAS assembly
7-8
Optical System
4. Install and secure the low angle diaphragm and the rear light focus lens assembly as
needed.
5. Install the LAS assembly to the optical support and screw it with the hexagon wrench.
During securing, ensure that the diaphragm assembly is close to the M4 hexagon screws.
MAS assembly
7-9
Optical System
4. Install and secure the diaphragm and the rear light focus lens assembly as needed.
5. Install the MAS assembly to the optical support and screw it with the hexagon wrench.
During securing, ensure that the diaphragm assembly is close to the M4 hexagon screws.
3PD
4PD cover
4. Use the cross-headed screwdriver to loosen the low angle pre-amplification board, remove
the PD board, replace the corresponding parts and reinstall them orderly.
5. Assemble the shielding box to the forward low angle PD assembly and then install the
forward low angle PD assembly to the optical support.
Optical System
assembly and remove the assembly from the optical support.
3. Use the cross-headed screwdriver to remove the two screws fixing the PD board and then
remove the shielding box from the forward middle low angle PD assembly.
3PD
4PD cover
4. Use the cross-headed screwdriver to loosen the low angle pre-amplification board, remove
the PD cover and the PD board, replace the corresponding parts and install them orderly.
5. Assemble the shielding box to the forward middle low angle PD assembly and then install
the forward middle low angle PD assembly to the optical support.
Pre-amplification board
7-11
Optical System
7.3 Adjustment
Any assembly in the optical system should be adjusted after the installation once it has been
disassembled. The adjustment procedure can be simplified according to how the assembly
has been disassembled.
The adjustment to the optical system consists of coarse adjustment and fine tuning. Coarse
adjustment roughly aligns every optical assembly and makes them approximately on the same
optical axis, so that the signal can be created rapidly when standard particles are run. Fine
tuning ensures the best position of the optical system. An oscilloscope is used to observe the
signal waveform and the corresponding optical assembly position is finely tuned so that the
signal generated meets the adjustment standard.
Purpose:
Horizontally, the beam is upright to the rear light collimator lens. The collimator emergent far
field spot is in the vertical direction.
Principle:
The laser beam distributes symmetrically along the optical axis; the reflex fully covers the
incident light when the light enters vertically.
Procedure:
Install the rear light assembly first, and cling the rear light assembly closely to the pin stop at
the rear light position, as Figure 7-12 shows. Aim the pin stop of the front light assembly at the
optical support, secure the fixing screw (make sure the forward optical system attaches the
optical support and is still movable). Turn on the power of the laser, and turn the laser
assembly to make the far field spot of the emitting laser vertical. Attach the rear light collimator
assembly to the pin stop, use a hexagon wrench to check whether the light reflected from the
surface of the rear light collimator lens is on the same axis with the incident light and slowly
turn the front light assembly to make them on the same axis, and then secure the assembly, as
Figure 7-13 shows.
7-12
Optical System
7-13
Optical System
Purpose:
Adjusting the flow cell to irradiate the incident light to the surface of the flow cell vertically.
Principle:
That the flow cell is vertical to the incident laser uses the principle of that the reflex fully covers
the incident light when the light enters vertically.
Procedure:
Turn the flow cell into the optical path (do not position the square aperture detection area in the
optical path). Use a hexagon wrench to check whether the light reflected from the flow cell
surface is on the same axis with that of the incident light and adjust the flow cell slowly to
harmonize them and then secure the flow cell.
Purpose:
To make sure the front and the rear light is on the same axis approximately.
Principle:
The beam that goes through the main point of the lens will not change the transmitting
direction.
Procedure:
1. Remove the rear light collimator assembly; check whether the beam spot that passes
through the beam-splitting prism and the diaphragm on the MAS assembly is symmetrical.
Adjust the MAS assembly to make the beam spot symmetrical, as shown in Figure 7-15.
7-14
Optical System
Figure 7-15 Adjustment of the horizontal position of the rear light collimator assembly
2. Move horizontally the rear light collimator assembly along the pin stop and observe the
beam spot position on the diaphragm of the MAS assembly. When the spot is in the center
of the diaphragm (as shown in Figure 7-16), secure the fixing screw of the rear light
collimator assembly.
Figure 7-16 The adjustment of the horizontal position of the rear light collimator
assembly is finished
The rear light assembly must cling to the pin stop when it is moved.
Optical System
Purpose:
To obtain the effect of selecting low angle scattered light by positioning the LAS assembly
diaphragm symmetrically along the light axis.
Procedure:
1. Place a piece of white paper with rough surface at the back of the LAS assembly
diaphragm, and adjust the horizontal adjusting screw cap until the spot is symmetrical
horizontally (as shown in Figure 7-17).
2. Adjust the vertical adjusting screw cap until the spot is symmetrical vertically (as shown in
Figure 7-17).
Purpose:
To obtain the effect of selecting middle low angle scattered light by positioning the MAS
assembly diaphragm symmetrically along the light axis.
Note: After the adjustment of the horizontal position of the rear light collimator assembly is
finished, the position adjustment of the MAS assembly diaphragm is finished. You can secure
the fixing screw.
Purpose:
To ensure the scattered light from the forward low angle is completely collected by the PD.
Procedure:
7-16
Optical System
Adjust the horizontal and vertical positions of the forward low angle PD assembly until the
center of the spot emitted from the LAS assembly diaphragm is completely received by the
aperture (as shown in Figure 7-18).
veiling glare
Forward middle low angle PD assembly adjustment
Adjust the forward middle low angle PD assembly in the same way as adjusting the forward
low angle PD assembly. Ensure that the center of the emitted spot is completely received by
the aperture (as shown in Figure 7-19).
Figure 7-19 Spot from the forward middle low angle PD assembly
1Aperture diaphragm of eliminating
veiling glare
The above description is a complete procedure of coarse adjustment. By the adjustment above,
each assembly is roughly positioned symmetrically along the light axis. The following
describes the fine tuning of the optical system.
7-17
Optical System
Place a card (with a drawing line on it) on the MAS assembly diaphragm so that the two
sideline shades of the flow cell distribute symmetrically along the drawing line, as shown in
Figure 7-21. Adjust the position of the diaphragm against direct light. When the shielded spot
distributes symmetrically along the drawing line, the adjustment is finished.
7-18
Optical System
1Beam spot
3Drawing line
DC background method:
Output the pre-amplification board TPOUT test point of the forward low angle PD assembly (or
the TP43 LASIN test point connected to the signal processing board) to the oscilloscope and
select DC gear. Adjust the position of the diaphragm against direct light until minimum LAS DC
background is obtained, and then the adjustment is finished.
After the adjustment, secure the two screws of the diaphragm against direct light (as shown in
Figure 7-22).
Optical System
light of the rear light assembly from the designed angle. Therefore, the tuning is mainly on the
forward optical system axial position, the flow cell horizontal position and the position of the
rear light collimator assembly.
Before the particle adjustment, at the Main Menu Setup Gain screen, set the
values of SS and FS to be 100.
Purpose:
Procedure:
1. Test the voltage of the tp2vref test point of the laser control board to see whether the
voltage reaches 4V. If not, adjust the variable resistance of the laser control board until the
voltage is 4V.
2. Output the pre-amplification board TPOUT test point of the forward low angle PD
assembly to the oscilloscope channel 1 (CH1), set the oscilloscope to be: voltage 50mv/div,
time 500ns/div and AC coupling.
3. Adopt standard particles as sample for test. Add 3 drops of Duke (4K-07) 7m standard
particles into a 1.5mL centrifugal tube, and then add diluent or deionized water into the
centrifugal tube to 1mL. Use the diluted standard particle solution within 3 hours.
4. Run the standard particles in the open-vial mode normally, check the signal in the
oscilloscope channel 1 and adjust the adjusting knob of the front light assembly (as shown
in Figure 7-23) in the axial position. When the maximum signal amplitude and stability is
obtained and distributes normally, the pulse width is the narrowest and about 1.3us, as
shown in Figure 7-24, secure the fixing screws of the front light guide assembly.
7-20
Optical System
Figure 7-24 Standard particle signal graph output by forward optical system
Note: The sampling time of the DIFF channel is about 20s. To simplify the adjustment and
generate signals quickly, you can adjust the forward optical axis adjusting knob in advance
until the distance between the front end surface of the front light guide assembly and that of
the guide assembly support is about 4mm.
Purpose:
To ensure that what the PD assembly detects is the scattered light signals of the designed
collection angles.
7-21
Optical System
Procedure:
1. Output the pre-amplification board TPOUT test point of the forward middle low angle PD
assembly (or the TP44 MASIN test point connected to the signal processing board) to the
oscilloscope channel 2 (CH2), set the oscilloscope to: voltage 100mv/div, time 500ns/div
and AC coupling.
2. Run standard particles in the open vial mode normally, adjust the adjusting knob of the
rear light collimator assembly and check the signal pulses of the oscilloscope channel 2.
Secure the set screws of the rear light collimator assembly when the pulse peaks reach
the maximum value, the signal are stable and the shake is little.
3. Switch to the channel 1 (CH1) and check whether the signal peaks are 190mv10mv. If not,
adjust the adjustable resistance on the laser control board until the signal peaks of the
CH1 are 190mv10mv.
4. Check whether the signal peaks of the channel 2 (CH2) are about 800mv. If not, adjust the
adjusting knob of the rear light collimator assembly and confirm whether the pulse signal
peaks of the CH2 are at the maximum.
Purpose:
To ensure that the center of the sample flow is positioned in the center of the beam spot.
Procedure:
1. Run standard particles in the open vial mode, and check whether the signal peaks of the
oscilloscope channel 1 (CH1) are stable and shake little.
2. Adjust the flow cell horizontal position until the particle signal peaks displayed on the
oscilloscope are at the maximum and uniform.
7-22
Optical System
Optical System
7-24
Optical System
the
gains,
enter
the
gains
into
the
corresponding
boxes
of
the
MainSetupGain screen and save the results according to the prompt on the screen.
After the gain setup, retest the calibrator and 7m standard particles to verify the gain and
check if the following requirements are met. For the calibrator: the low angel gravity center of
the calibrator should be within 0.57 from the target; the high angel gravity center within 0.63
from the target. For the 7um standard particles: the low angel gravity center of the calibrator
should be 18.051.37 (16.68, 19.42); high angel gravity center 104.243.68 (100.56, 107.92);
low angel width 6.50; high angel width 15.21. Otherwise, continue calibrating until the
following requirements are met. For the calibrator: the low angel gravity center of the calibrator
should be within 1.51 from the target; the high angel gravity center within 2.4 from the target.
For the 7um standard particles: the low angel gravity center of the calibrator should be
18.051.37 (16.68, 19.42); high angel gravity center 104.243.68 (100.56, 107.92); low angel
width 6.50; high angel width 15.21.
Sample verification
After the above standard particle gain setup is finished, get several normal fresh blood
samples for test. The scattergram of a normal fresh blood sample should be like the images
shown in Figure 7-29:
7-25
Optical System
7-26
Optical System
7.4 Troubleshooting
7.4.1 Laser spot-deviation
The SS and FS gain setup is not changed during the use of the analyzer. Yet the scattergram
is compressed to the left down side, namely, the two signals collecting angle direction turn
smaller, as shown in Figure 7-30. This is mainly because the focusing spot at the flow cell
position move forward or backward, causing the diminishment of laser intensity.
Settlement:
Make standard particle fist, and record the peak value position of standard particle. Loose the
laser axial adjustment lock screws (as shown in Figure 7-20). Turn the adjustment screw of
front light assembly counterclockwise for a quarter turn. Make standard particle and see the
peak value position. If the peak value position becomes greater, keep turning the adjustment
screw counterclockwise with 1/8 circle each time till the peak value position becomes smaller,
then turn back with 1/8 circle. If the peak value position becomes smaller, turn the adjustment
screw in the reverse order with the same method. After finding the maximum position of
standard particle, lock the laser axial adjustment fixing screws.
7-27
Optical System
7-28
Troubleshooting
Error Name
Error Code
Error Name
0x01000100
0x01003011
High pressure
0x01000101
0x01003020
0x01000201
0x01003021
position
0x01000202
0x01003030
0x01003031
motor
to
0x01003032
target
position failed
0x01000205
motor
to
0x01003033
0x01003050
0x01003060
0x01003061
0x01003062
0x01003070
Diluent expired
home
position failed
0x01000206
motor
to
leave
0x01000208
0x01000209
0x01000211
8-1
Troubleshooting
motor failed to move to target
position
0x01000212
0x01003071
0x01003072
0x01003073
0x01003074
Cleanser expired
0x01003080
No Diluent
0x01003081
No LH lyse
0x01003082
No LEO(I) lyse
0x01003083
No LEO(II) lyse
0x01000214
motor
to
target
position failed
0x01000215
motor
to
upper
position failed
0x01000216
0x01000217
0x01000218
0x01000219
0x01000220
0x01003084
No Cleanser
0x01000221
Cannot
sampling
0x01003085
Waste is full
0x01003090
0x01003091
0x010030A0
adjust
assembly
0x01000222
0x01000224
voltage abnormal
position
0x01000225
0x010030A1
+12V
analogue
voltage
abnormal
0x010030A2
-12V
analogue
abnormal
8-2
voltage
Troubleshooting
0x01000227
mismatch
0x010030A3
current
Drive
board
24V
voltage
12V
voltage
5V
voltage
abnormal
position
0x01000228
0x010030A4
Drive
board
abnormal
position
0x01000229
0x010030A5
Drive
board
abnormal
position
0x01000230
0x010030B0
0x010030B1
Data
0x01000232
Sampling
board
FLEXBUS
abnormal
assembly
WBC
0x010030B2
0x010030B3
Data
photocoupler error.
0x01000234
Sample
assembly
Sampling
vertical
0x010030B4
assembly
Sampling
Data
board
AD7928
abnormal
upper
0x010030C0
photocoupler error
0x01000236
AD7908
abnormal
board
assembly
lower
0x010030D0
0x010030D1
0x010030D2
photocoupler error
0x01000300
0x01000301
Sampling
syringe
photocoupler abnormal
0x01000302
open
0x01004200
0x01004201
Response overtime
0x01004202
Heartbeat disconnected
0x01004401
0x01004402
home
position
after
action
0x01000304
0x01000305
0x01000306
Sample
syringe
aspirate
8-3
Troubleshooting
volume over range
0x01000307
Sample
syringe
dispense
0x01004403
action
0x01004404
0x01000310
0x01004405
0x01000311
Sample
0x01004406
0x01004407
0x01004408
0x01004409
0x0100440a
0x01004600
Analyzer
Sample
syringe
overtime
injection
syringe
photocoupler abnormal
0x01000312
Sample
failed
injection
to
return
syringe
to
home
Sample
injection
syringe
Sample
failed
injection
to
return
syringe
to
home
Sample
injection
syringe
Sample
injection
syringe
Sample
injection
disconnected
syringe
0x01004601
Sample
injection
communication
Analyzer
network
configuration fail
syringe
0x01005200
action overtime
0x01000320
0x01005201
0x01000321
Sheath
0x01005202
Manipulator
fluid
syringe
photocoupler abnormal
0x01000322
0x01000323
pinch
motor
0x01005203
Manipulator
pinch
motor
action
position
home
position
0x01005204
after
Manipulator
pinch
motor
action forbidden
action
0x01000324
0x01005205
Manipulator
pinch
motor
initialization failed
action
0x01000325
0x01005206
8-4
Manipulator
pinch
motor
Troubleshooting
leave home position before
action overtime
action
0x01000326
0x01005207
0x01005208
motor
Manipulator
pinch
motor
0x01005400
overtime
0x01000330
pinch
Manipulator
Manipulator
pinch
motor
initialization forbidden
0x01005401
0x01000331
Lyse
syringe
photocoupler
0x01005402
abnormal
0x01000332
0x01005403
0x01000333
0x01005404
action forbidden
0x01005405
0x01005406
0x01005407
0x01005408
out of range
0x01000338
out of range
0x01000337
0x01005500
0x01000340
0x01005501
0x01000341
0x01005502
abnormal
0x01000342
home position
0x01005503
0x01005504
0x01005505
0x01005506
0x01005507
0x01000345
0x01000346
Diluent
syringe
aspirate
8-5
Troubleshooting
volume out of range
0x01000347
Diluent
syringe
position
dispense
0x01005508
Diluent
syringe
action
0x01005600
overtime
0x01000401
0x01005601
0x01005602
limit
0x01000402
0x01000601
failed
0x01005603
Mix
mechanism
action
overtime
0x01000602
0x01005604
0x01000603
0x01005605
pressure failed
0x01000604
failed
0x01005606
vacuum failed
Mix
mechanism
current
action forbidden
0x01000605
0x01005700
0x01000606
0x01005701
0x01000607
0x01005702
error
0x01005703
of control limit
0x01000611
0x01005704
0x01000701
0x01005705
0x01005706
Feeding
symbol error
0x01000702
0x01000703
Driver
board
motor
action
overtime
FPGA
load
0x01005708
0x01005801
out of limit
0x01000705
code error
0x01005802
limit
0x01000706
0x01005803
0x01000707
Drive
board
FPGA
write
0x01005804
8-6
Troubleshooting
overtime
0x01000731
error
0x01005805
error
0x01000801
error
0x01005806
error
0x01000802
board
identity
board
FPGA
code error
0x01005820
Autoloader
0x01005821
error
0x01000804
Autoloader
board
FPGA
0x01005822
0x01000805
0x01005823
0x01000806
0x01005824
0x01000807
0x01005825
Autoloader
board
write
board
write
forbidden
0x01000808
0x01005826
Autoloader
overtime
0x01000809
0x01005900
error
0x01000810
0x01005901
receive
0x01005902
error
0x01000812
motor
command error
number error
0x01000811
Loading
0x01005903
0x01000813
0x01005904
error
0x01000814
0x01005905
unloading
Unloading
position
right
photocoupler error
0x01005906
error
0x01000816
motor
error
0x01000815
Loading
Unloading
position
left
photocoupler error
pack
execution
0x01005907
0x01005908
overtime
0x01000817
error
0x01002000
Background abnormal
initial position
0x01005909
8-7
Troubleshooting
0x01002010
0x0100590A
Loading
motor
action
overtime
0x01002011
0x0100590B
0x01002020
0x0100590C
0x01002021
0x0100590D
0x01002022
WBC bubbles
0x0100590E
0x01002030
0x0100590F
0x01002031
0x01005a00
0x01002040
0x01005a01
Opening
sample
0x01005a02
0x01002042
RBC bubbles
0x01005a03
0x01003000
Low vacuum
0x01005a04
Autoloader
parameter
adjustment
setting
range
0x01003001
High vacuum
0x01003010
Low pressure
0x01005a05
8-8
out
of
Troubleshooting
Description
not
meet
Possible causes
the
requirement.
failed
not
meet
the
requirement.
No LH lyse
Description
Possible causes
pick
up
tube
is
connected improperly.
needs to be replaced.
LH lyse is used up or
LH
excessive bubbles.
8-9
lyse
channel
leaks,
leading
to
Troubleshooting
No LEO(I) lyse
in
excessive bubbles.
the
LEO(I)
lyse
channel
No LEO(II) lyse
or
excessive
bubbles
excessive bubbles.
channel
No Cleanser
Waste is full
Cleanser is used up or
Cleanser
excessive bubbles.
full
sensor
waste
or
connecting
the
the
container is connected
channel
leaks,
leading
to
needs to be replaced.
improperly
Diluent expired
LEO(I) Lyse Expired
LEO(II) Lyse expired
LH Lyse expired
Cleanser expired
Diluent expired
Diluent expired
Exp. date setting is wrong
LH lyse expired
LH Lyse expired
Exp. date setting is wrong
Cleanser expired
Cleanser expired
Exp. date setting is wrong
Description
Possible causes
Background
abnormal
8-10
Troubleshooting
4. Bubbles or clog is alarmed during the
background count.
5. Outside interference caused by poor
shielding
LAS
background
voltage abnormal
HGB
background
voltage abnormal
out
of
the
range
[3.2,4.9]V
RBC clog
RBC bubbles
WBC clog
8-11
Troubleshooting
aperture is clogged by impurity.
3. The photocoupler of the volumetric
tube is damaged.
4. Vacuum abnormal
5. Volumetric-tube-related tubing clogs.
6. Poor connections between the valves
and the driver board.
WBC bubbles
Description
Possible causes
error
40] .
or
bath
transducer
DIFF
reaction
bath
temp. error
reaction
temperature
error
requirement.
The temperature transducer of reaction
bath is damaged and needs to be
replaced.
Ambient
temperature
Ambient
abnormal
temperature
temperature
or
ambient
transducer
error
requirement
The ambient temperature transducer is
damaged and needs to be replaced.
8-12
Troubleshooting
Ambient
temperature
Ambient
temperature
abnormal
or
temperature
ambient
transducer
error
requirement
The ambient temperature transducer is
damaged and needs to be replaced.
Description
Possible causes
Inaccurate volume of DIFF differential
division
MON
and
NEU
range.
The
reaction
is
not
thoroughly processed.
For normal samples, the
DIFF
scattergram
obtained
from
the scattergram
range.
The
reaction
is
not
thoroughly processed.
Sheath flow is not formed. Excessive
bubbles enter the WBC channel. Check
whether the diluent container is empty to
make sure if something is wrong with the
diluent sensor.
Calculate the gain of the analyzer
For
Compressed
scattergram
normal
patient
the
samples,
scattergrams obtained is
scattergram
compressed
either
differentiates
properly
8-13
Troubleshooting
problem, refer to the optical system
section in the service manual to remove
the error.
8-14
Appendixes
A. Accessories
A.1
No.
Name
3001-30-68501
Data Board
3101-30-68503
Drive Board
3101-30-68505
Mother Board
3101-30-68507
3101-30-68509
3101-30-68511
Volumetric Board
3101-30-68513
3101-30-68515
FS Pre-amplify Board
3101-30-68517
SS Pre-Amplify Board
10
3101-30-68519
Indicator Board
11
3102-30-69199
Autoloader board
12
0033-30-74615
10ml syringe
13
3100-10-49438
100ul syringe
14
3101-10-69301
250ul syringe
15
M90-100032---
2.5ml syringe
16
3003-20-34942
2-way valve(Asco)
17
3003-20-34941
3-way valve(Asco)
18
3101-30-68611
19
M07-00014S-00
20
3101-30-68351
21
3001-10-07252
Waste Pump
22
530B-10-05275
Pressure Pump
23
3001-10-07057
Volumetric Tube
24
3100-30-40745
25
3101-30-68320
26
3102-30-69230
HGB assembly
27
3102-30-69253
Appendixes
28
3102-30-69223
29
3003-20-53969
30
3003-20-53970
31
3102-20-69171
Sampling probe
32
3101-30-68756
33
3101-30-68765
34
3101-30-68766
35
3101-30-68767
36
3101-30-68768
37
3101-30-68776
38
3006-20-74804
Probe Wipe
39
3003-20-34949
Isolation Chamber
40
3102-20-69165
Pressure Chamber
41
3101-30-68547
Vacuum Chamber
42
3001-10-07054
Air filter
43
2800-21-28878
44
3003-21-34925
45
0030-10-13064
46
BA30-10-15115
Sampling motor
47
0000-10-11272
48
0000-10-11271
49
3102-30-69231
autoloader assembly
50
3101-30-68651
51
3100-30-49556
52
3100-30-49559
53
3100-30-49560
Splitter Assembly
54
3101-30-68662
55
3101-30-68663
56
3101-30-68664
Las PD Assembly
57
3101-30-68665
Mas PD Assembly
58
3100-30-49568
59
3100-10-49436
60
M90-100031---
61
M90-000026---
62
M90-000025---
Appendixes
63
M6G-020011---
64
M6G-020009---
Tubing, 0.031"ID0.156"OD
65
M6G-020007---
66
M6G-020006---
67
A21-000007---
Tubing, Silicone,3/32"X7/32"2800392-100
68
3001-10-07069
Tubing,1/16"X1/8",S-50-HLAAX02002,Tygon
69
0040-10-32301
Tubing,PTFE,.040"ID+/-.002"X.066"OD+/-.002
70
3101-20-68590
71
3101-20-68572
72
M07-00097S---
Switch
73
3001-10-07060
Aspirate key
A.2
No.
Spare pipes
Code/Model
Name
0040-10-32301
Tubing.PTFE,.040""ID+/-.00
3001-10-07069
Tubing.1/16"X1/8",S-50-HLAAX02002,Tygon
A21-000002---
Tubing.Silicone,1/8"X1/4" X100ft,2800546-100
M6G-020006---
Tubing.Silicone,1/16""X3/1
M6G-020007---
M6G-020009---
M90-000025---
Tubing.1/8""X1/4"",R-3603
A.3
No.
1
2
3
4
5
6
7
8
9
10
11
12
Spare PCBA
Code/Model
051-000014-00
3101-30-68503
3101-30-68505
3101-30-68507
3101-30-68509
3101-30-68511
3101-30-68513
3101-30-68515
3101-30-68517
3101-30-68519
3102-30-69197
3102-30-69199
Name
Data board (including rootfs)
Drive board
Mother board
Liquid-level board
Power board
Volumetric board
Laser control board
FS pre-amplification board
SS pre-amplification board
Indicator board
Key board
Autoloading board
A-3
Appendixes
A.4
No.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
A.5
No.
Spare cables
Code/Model
3100-20-49018
3100-20-49040
3101-20-68574
3101-20-68575
3101-20-68578
3101-20-68581
3101-20-68584
3101-20-68585
3101-20-68589
3101-20-68590
3101-20-68591
3101-20-68592
3101-21-68588
3101-21-68593
3102-20-69101
3102-20-69102
3102-20-69104
3102-20-69105
3102-20-69106
3102-20-69107
3102-20-69108
3102-20-69103
Name
Network cable (for connection between the analyzer and the
computer)
Connecting cable of Micro-switch
Connecting cable of reagent sensor
Connecting cable of volumetric board
Connecting cable of power switch
Connecting cable of laser control
SS signal line
FS signal line
Connecting cable from switch to power board
Power box fan and connecting cable
Outlet earth wire
Connecting cable of pump
Connecting cable of AC input
Connecting cable of laser
Power cord of the autoloading unit
Connecting cable of autoloader control
Wiring harness of the mixing motors
Connecting cable of DIFF bath
Connecting cable of the indicator board
Connecting cable of the switch control
Connecting cable of the autoloading motors and sensors
of the autoloading motors and sensors
Spare connectors
Code/Model
Name
3001-10-07066
3102-20-69219
M90-100009---
M90-100010---
Nylon
M90-100012---
M90-100013---
M90-100015---
Lock Nut
M90-100016---
Male
Luer
Lock
Ring,
For
MTLP
or
M90-100017---
LC23,Orange
10
M90-100019---
11
M90-100020---
Appendixes
12
M90-100021---
13
M90-100022---
14
M90-100023---
15
M90-100024---
16
M90-100025---
17
M90-100028---
Connector.Tee,400Barb,3/32"ID,White Nylon
18
M90-100028-01
19
M90-100028-03
Connector.Y,400Barb,3/32"ID,White Nylon
20
M90-100030---
Connector.Y,200Barb,1/8"ID,White Nylon
21
M90-100046---
22
M90-100048---
23
M90-100049---
24
M90-100051---
25
M90-100052---
26
M90-100065---
27
M90-100069---
Connector.Thread,10-32UNF,1/4"Hex,400Barb,3/32"ID
A.6
Consumables
No.
Name
Code/Model
Package
Specifications
1
2
3
4
5
6
7
8
9
M-53LEO(I)
Lyse(Chinese/4L)
M-53LEO(I)
Lyse(Chinese/1LX4)
M-53LEO(I)
Lyse(English/1LX4)
M-53LEO(I)
Lyse(English/4L)
M-53LEO(II)
Lyse(Chinese/200mlX4)
M-53LEO(II)
Lyse(Chinese/400mlX4)
M-53LEO(II)
Lyse(English/200mlX4)
M-53LEO(II)
Lyse(English/400mlX4)
M-53LH
Lyse(Chinese/500mlX4)
A11-000105---
4LX1
A11-000113---
1LX4
A11-000128---
1LX4
A11-000129---
4LX1
A11-000114---
200mLX4
A11-000115---
400mLX4
A11-000130---
200mLX4
A11-000131---
400mLX4
A11-000116---
500mLX4
A-5
Appendixes
10
11
12
13
14
15
16
17
M-53LH
Lyse(Chinese/1LX4)
M-53LH
Lyse(English/500mlX4)
M-53LH
Lyse(English/1LX4)
M-53
Cleanser(Chinese/1LX4)
M-53
Cleanser(English/1LX4)
M-53D
Diluent(domestic/20L)
M-53D
Diluent(export/20L)
M-50P Probe Cleanser
domestic/50mL
18
A11-000117---
1LX4
A11-000132---
500mLX4
A11-000133---
1LX4
A11-000118---
1LX4
A11-000134---
1LX4
A12-000140---
20LX1
A12-000167---
20LX1
A12-000151---
A12-000178---
A-6
50mLX1
50mLX1
Code/Model
Name
3001-10-07054
Air Filter
3003-20-34941
3003-20-34942
3006-20-74804
Probe Wipe
M07-00014S-00
M07-00076F---
FUSE Slow-Blow
M07-00077F---
M07-00079F---
M08-000789---
PHOTOELEC switch
10
M6G-020011---
Tubing.PharMed,1/16""ODX1/
B-1
C. Fluidic diagram
C-1
No.
V01
V02
V03
V04
Function
Provides fluid for the sample probe; V01 opens, the waste discharging
path of the diluent syringe connects the sample probe.
Provides fluid for the probe wipe; V02 opens, the waste discharging
path of the diluent syringe connects the inlet of the probe wipe.
Aspirating/discharging switch of diluent syringe
Cleans the interior of the sample probe; V04 opens, the sample probe
connects the sheath fluid syringe.
Two
way/Tee
3
3
3
2
V05
V06
V07
V08
V09
V10
V11
V12
V13
Provides fluid for RBC bath; V08 opens, the waste discharging path of
the diluent syringe connects the fluidic line of RBC bath
Opens and closes the pressure chamber and the waste outlet of the
DIFF bath; bubbles the DIFF bath
Opens and closes the path between the vacuum chamber and the
pressure chamber
Opens and closes the pressure chamber and the isolation chamber t of
the RBC bath; bubbles the RBC bath
Opens and closes the pressure chamber and the isolation chamber t of
the WBC bath; bubbles the WBC bath
Together with V33 and V29 to control the cleaning of the WBC back
bath
3
2
2
2
2
2
V14
Together with V34 and V30 to control the cleaning of the RBC back bath
V15
V21
V22
V23
V24
V25
Opens and closes the sheath fluid syringe and the sample dispensation
syringe; assists the sheath fluid dispensing the sample
Opens and closes the sheath fluid syringe and DIFF bath; cleans DIFF
bath
Aspirating/discharging switch of sheath fluid syringe
Opens and closes the sheath fluid syringe and flow cell; dispenses
sheath fluid
Empties the WBC volumetric tube
D-1
2
2
3
2
2
Appendixes
V26
V27
V28
V29
V30
V31
V32
V33
V34
V35
V36
V37
V38
V39
D2.
Name
Function
Creates pressure in the pressure chamber, which is used to bubble and flash
P1
the aperture
Discharges the waste of the DIFF bath; empties and creates vacuum in the
P2
vacuum chamber to drive the counting by impedance method, clean the back
bath and drain the volumetric tube.
Discharges the waste of the WBC bath, RBC bath and probe wipe
P3
D3.
Diluent syringe
Sheath syringe
Aspiration
Function
Dispenses diluent into the WBC bath and RBC bath; cleans the sample
probe and dispenses fluid for the probe wipe
Prepares samples; forms sheath fluid; cleans the DIFF bath and the
sample preparation tubing
Aspirates and dispenses samples
syringe
Sample syringe
Lyse-Syringe
Aspirates and dispenses the LEO (I) Lyse, LEO (II) Lyse and LH Lyse
D-2
E. Tubing
E1.
Components
E-1
1. With the metal contactors side up, mark the 8 lines in the connector from left to right with 1-8.
Accordingly, the colors of the lines are: white and orange, orange, white and green, blue, white
and blue, green, white and brown, and brown.
F.2.
1. With the metal contactors side up, mark the 8 lines in the connector from left to right with 1-8.
Accordingly, the colors of the lines are: white and orange, orange, white and green, blue, white
and blue, green, white and brown, and brown.
F-1
Appendixes
F-2
P/N: 046-000142-00(V1.0)