Professional Documents
Culture Documents
Technology Overview
Advisory Committee
for
Pharmaceutical Science
July 19, 2001
Compendial Methods
USP
Plate Counts- Colony Forming Units
Growth of microorganisms on solid medium
Count visible colonies
ACPS
Compendial Methods
ADVANTAGES
Simple
Counts only viable
organisms
July 19,
DISADVANTAGES
Incubation time (48-72
hours)
Not all organisms will
grow on a single
medium
ACPS
Microbial Viability
Only interested in viable microorganisms
New methods must only count viable
organisms
Viability indicators needed
July 19,
ACPS
Esterase Detection
ChemScan, Chemunex
July 19,
ACPS
ChemScan Procedure
Filter Sample
Expose membrane to reagent
Membrane analyzed by laser-scanning
July 19,
ACPS
ATP Bioluminescence
MicroStar(TM) & MicroCount(TM), Millipore
July 19,
ACPS
MicroStar(TM) Procedure
Filter Sample
Place membrane on solid medium for brief
incubation
Disrupt cells to release ATP
Add bioluminescence reagent to membrane
Detect light with CCD and computer
analysis
July 19,
ACPS