Name:___________________________

Lab
:YeastPopulations

LabPartners:__________________

Background:
Yeastisasinglecellfungusthatproducescarbondioxideasabyproductofcellularrespiration.
Thereleaseofcarbondioxidecausesbreaddoughtorise.Becausetheyeastsaresmallandreproducerapidly,
yeastorganismsareusefulforstudyingvariousfactorssuchasfoodavailability,temperaturechangeandashift
inpHthatmayinfluencetherateatwhichapopulationgrows.Theoptimumtemperatureforyeastmetabolism
andyeastreproductionisbetween30and35degreesCelsiusdependingonthespeciesandprovidingthereisan
adequatefoodsupply.Thesecellsproducecarbondioxidegasasawasteproductandtheamountofcarbon
dioxideisanindicationofyeastgrowth.

Objectives
:Afterreadingthebackground,youwillbeexpectedtodothefollowing:

Formulateahypothesisaboutyeastpopulationgrowth
Designandconductanexperimenttotestyourhypothesis
Organizethedatafromyourexperimentinatableandgraph
Evaluateyourhypothesisbaseduponyourdata
Evaluateanddiscussexperimentaldesignandconclusion

Experiment
:Reviewthelistofmaterialsprovided.Thesewillbeusedtodesignyourexperiment
1.a.Problem:Inthespacebelowwritetheproblemfortheexperiment.

Doesthetemperatureaffectthecarbondioxidelevelsthattheyeastproduces?

b.Hypothesis.Inthespacebelow,writeahypothesisfortheproblemstatedabove.Letsmakesurethatgroups
havedecidedonwhichfactorstheywillinvestigate.BesuretowriteyourhypothesisasanIf
(independentvariable)then(dependentvariable)because...statement.

Iftemperaturedecrease,thenthesizeofthecarbondioxidebubblewilldecrease,becauseyeast
metabolismandyeastreproductionisbetween30and35degreesCelsius.

2.IdentifyyourVariables

a.IndependentVariable:Temperature(C)

b.DependentVariable:CarbonDioxideLevels(cm)

c.FactorsHeldConstant:VolumeofMolasses,mLofYeast,Time
(listatleast3)


3.Procedure:
1. Place7mLof25%molassessolutionintoasmalltesttube.
2. Stir the yeast suspension and then place 1 mL of the yeast suspension into the same test
tube.
3. Placethetesttubeintherack.
4. Wash and rinse hands. Place a palm over the end of the small test tube and invert it five
times.
5. Carefully slide a larger tube down over the smaller tube. Quickly invert the tubes so the
mouthofthelargetubeisup.
6. Using a metric ruler measure the height of the air bubble (cm) in the smaller tube and
record.Placeinthetesttuberack.
7. Repeatsteps16fortwomoresetups.
8. Leaveonesampleat30degreesCelsiusfor24hours.
9. Leaveonesampleatroomtemperaturefor24hours.
10. Leaveonesampleinarefrigeratorfor24hours.
a. Measurethebubbleandrecordthe
changeinthesizeduetocarbondioxidegasproduction
on
yourdatatable.(Subtracttheinitialgasbubblesizefromthetotalbubble.)

Drawasketchofhowyouwillsetupanexperimentthatwilltestyourhypothesis,and
labelallparts
of
yourexperimentalandcontrolsetups.

4.Data:
a.Makeatableinthespacebeloworonaseparatesheetofgraphingpaper.Makesureyourtablehas
descriptiveheadings.Useittorecordyourdata.Datamayalsobequalitativeaswellasquantitative.

Temperature
(C)

Changein
HeightBubble
(cm)Trial1

Changein
HeightBubble
(cm)Trial2

Changein
HeightBubble
(cm)Trial3

AverageChange
inHeight
Bubble(cm)

2.5cm

1.5cm

0.5cm

1.5cm

21

2cm

2,5cm

5.5cm

3.33333cm

30

4.5cm

4.5cm

4.5cm

4.5cm

b.Graph:usethespacebelowtocreateavisualrepresentationofyourdata.LABELallaxis!!

5.
Conclusion
.Inthespacebelow,statewhetheryourdatasupportsorrefutesyourhypothesis.Conclusionsare
relatedtothestatedproblemandhypothesisand
supportedbydata
andscientificconcepts.

Ourhypothesiswascorrect,whenthetemperatureincreasestheheightofthebubblewillincrease.When
thetemperaturewas4degreescelsius,theaveragechangeintheheightofthebubblewas1.5
centimeters.Whenthetemperaturewas21degreescelsius,theaveragechangeintheheightofthe
bubblewas3.333centimeters.Andwhenthetemperaturewasat30degreescelsius,theaveragechange
intheheightofthebubblewas4.5centimeters.

6.
Validity
:Validityisdiscussedintermsofwhatwasdoneandcouldhavebeendonetomaketheexperiment
morevalid.Howwelldidtheothergroupsconclusionsconcurwithyours?

Bymakingmoretrialstotesttheeffectoftemperaturemighthavemadetheresultsmoreprecise.Addingthe
trialswillensureareliableexperiment.Wecouldhaveincreasedordecreasedthetemperaturestogetmore
averagesontheheightofthebubble.Wecould
of
alsohavetrieddoingmorevariablesinvolvingtimelike

waitingmoredaystoseeiftheratiooftheeffectontheheightofthebubblesisstillthesame.Thiscould
improveourvaliditybecausewewilltrulybeabletoseeifourdataisspoton.

7.
RealLifeApplication
:Howdoesthislabrelatetoreallife?

Ifwhenweremakingbread,weneedtoplacetheyeastinawarmcontrolledenvironmentin
orderfortheyeasttoadapttothewarmthofbread.Inthislabtheyeastwasinacontrolled
roomtemperaturesothatitcouldrise.