Conc (nM)

0.00
3.00
5.00
7.00
9.00
11.00

Signal
10.00
20.00
30.00
40.00
50.00
60.00

Ymin
Ymin
Cmin
Cmin

10.60
12.78
0.20
0.68

9.75
21.32
32.3
39.65
47.55
61.35

9.25
19.15
28.75
38.26
49.5
58.75

Mean
Std Dev.
9.67
0.312
20.16
0.893
30.35
1.470
39.30
0.751
49.02
1.057
60.03
1.062

Conc (nM)
0.00
3.00
5.00
7.00
9.00
11.00

Ymin = Yblank + 3*s Yblank = mean

Ymin = Yblank + 10*s
S/N=3 Cmin = (Ymin-Yblank)/m
S/N=10 Cmin = (Ymin-Yblank)/m
m=
sm =
R2=

70.00
y = 4.6077x + 7.8763
R = 0.9949

60.00

Signal

50.00
40.00
30.00
20.00
10.00
0.00
0.00

Mean
9.67
20.16
30.35
39.30
49.02
60.03

2.00

4.00

6.00
Concentration (nM)

8.00

10.00

12.00

Std Dev.
0.31
0.89
1.47
0.75
1.06
1.06

4.61
0.17
0.99

7.88 b =
1.14 sb =
1.49 sy =