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  • Vibrio Eng

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    Melih Bayar
    20 views14 pages
    Cholera is a General Hazardous disease Ranked by the international quarantine disease. Cholera toxin encodes a VPI (Vibrio pathogenicity island), which is the necessary genes for TCP, as well as the return pilus cholera toxin other weaker virulence factors. There is a slide title epidemics Appears extent that, in several countries and continents pervasive long lasting epidemics and pandemics.

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    Cholera is a General Hazardous disease Ranked by the international quarantine disease. Cholera toxin encodes a VPI (Vibrio pathogenicity island), which is the necessary genes for TCP, as well as the return pilus cholera toxin other weaker virulence factors. There is a slide title epidemics Appears extent that, in several countries and continents pervasive long lasting epidemics and pandemics.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    20 views14 pages

    Vibrio Eng

    Uploaded by

    Melih Bayar
    Cholera is a General Hazardous disease Ranked by the international quarantine disease. Cholera toxin encodes a VPI (Vibrio pathogenicity island), which is the necessary genes for TCP, as well as the return pilus cholera toxin other weaker virulence factors. There is a slide title epidemics Appears extent that, in several countries and continents pervasive long lasting epidemics and pandemics.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 14

    9/10/2015

    Thereisaslidetitle

    Page1

    Vibrio

    FacultyofAgricultureandForestry

    Page2

    Vibriocholerae
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    Thereisaslidetitle

    Thecauseofcholera

    GeneralHazardousdisease

    Rankedbytheinternationalquarantinedisease

    DiagnosisrequiresimmediatenotificationTHLand
    WHO

    Evenoneincidentisclassifiedasanepidemic

    Page3

    Vibriocholerae

    Gramnegative
    Oneoftheflagellaofthecellfromthe
    Threadedrod

    Fermentsglucose,sucroseandmannitol
    Apositivelysineandornithinedecarboxylasetests
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    Theclinicalpicture

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    Page6

    Infectivedose

    1001000bacteria

    Riskgroups:malnourishedandsick

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    Virulencefactors

    Choleratoxin

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    (similartotheETECbacterium,theLTtoxin)

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    Thereisaslidetitle

    lysogenicbacteriophageCTX

    ItencodesaVPI(Vibriopathogenicityisland),whichis
    thenecessarygenesforTCP,aswellasthereturnpilus
    Choleratoxin

    otherweakervirulencefactors

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    Occurrence

    man
    animals
    foods
    surroundings

    MostintheFarEastandCentralAmerica
    Asymptomaticcarriers
    dogs,cowsoirettomiachickenscarriers
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    Thereisaslidetitle

    riversandbays

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    Factorsthatcouldleadtotheinfection

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    Epidemics

    Appearsextentthat,inseveralcountriesandcontinentspervasive
    longlastingepidemicsandpandemics

    Pandemicsdescribedinthe1800s
    6wasthefirstclassicalO1serotype
    7pandemicbeganinIndiain1961(ElTorbiotype)
    1991andspreadtoSouthAmerica
    8pandemicisO139Benegalbiotypeandstartedin1992
    IndiaandspreadfasterthanO1

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    Vibriocholerae

    IsdividedonthebasisoftheOantigenofmorethan200serogroup

    OnlyO1andO139serotypescausingepidemicsand
    panepidemioita

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    Thereisaslidetitle

    VibriocholeraewhichdonotagglutinateO1andO139
    antiserumclassifiedasnonO1,andnonO139Vibrio
    cholerae(nonagglutinableVibriocholeraeorVibrionon)

    Vibriocholeraenonpathogenscanbe,butarenot
    causeepidemics,butinfectionsandsepsis

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    Vibriocholeraedetection

    Vibriocholeraeismaintainedinthefaeces,ifitisdamp,
    ThesuspectfecalsampleistransportedinCaryBlair
    Transportbroth

    AtoastbytheTCBSplatform(Thiosulphatecitratebilesalts
    sucrose)
    Ink612h+TCBSmediumuusintamaljaus
    Ink1824htoVibriocholeraecolonieswerevisible
    thesoftyellowoflooks,thecenterofwhichiselevated
    oksidaasipositiivisettestedO1andO139sera

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    Page22

    Vibriocholeraerapidtest

    fecesunderamicroscope

    addedO1andO139antiserum

    ifitisfoundcirculatingmicrobialstoppingitshouldbe
    suspectVibriocholeraepositivesamples

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    Vibriocholeraesubtypes

    O1isdividedintotwobiotype
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    Thereisaslidetitle

    TheclassicandElTor

    Separationbytestinghemolysis,hemagglutination,
    phagetyping,polymyxinBsensitivity,andVoges
    Proscauerreaction.

    ThenewestwaytoPCRorotherbiotyyppispesifisigenes
    identified(TCPA
    andrtxC)

    Page24

    Vibriocholeraeinthesameserotype,andbiotype

    canbeseparatedbyphagetyping

    O1has145phagetype

    O139only5

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    Otherseparationmethods

    theenzymebyelectrophoresis

    ribotyyppauksella

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    Thereisaslidetitle

    Vibriospp

    Vibriocholerae
    V.parahaemolyticus
    Vibriovulnificus

    V.fluvialis
    V.mimicus
    V.alginolyticus
    V.hollisae
    A.Furniss

    Morethan20speciesareknown

    Page36

    V.parahaemolyticus

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    Page40
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    V.vulnificus

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    Thereisaslidetitle

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