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Thedetectionandidentificationofbacteria
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Moulds
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Mouldshavealotof
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Thedetectionandidentificationofbacteria
Mostproblemsarecaused:
Penicillum
Aspergillus
Fusarium
mycotoxinsproducers
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Moulds
Reproductiondependsprimarilyonthewater
availability
Bacteria>0.91
Mouldsalsofrom0.85to0.9
SomeEurotium0.75to0.8,andAspergillus
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Thedetectionandidentificationofbacteria
Thefoodismicrobiologicallystablewhenthewater
activityis<0.75
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HomeDamage
flavor
fragrance
structure
POISONRETURNiemycotoxins
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Mycotoxins
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Thedetectionandidentificationofbacteria
arenotPROTEIN
arenotenzymes
arelowmolecularweight<700gmol
thesecondarymetabolismproducts
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Whyisproduced
mycotoxins?
Mayactantifungisideina
Protectingmushroomsreproductiveorgans
Mostlynocertaintywhy
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Thedetectionandidentificationofbacteria
Allstrainsofthesamespeciesdonotproduce
Differentspeciescanproducethesamemycotoxin
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Wheremycotoxins
produced
Worldgrainproductionby2530%hasbeen
abreedinggroundformoldsandseen
Mycotoxins
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Tuohoutuukomycotoxins
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Thedetectionandidentificationofbacteria
easily?
Not
Mycotoxinsusuallydonotdisappearor
inactivatedinthemanufactureoffood
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Cerealalreadyinfectedinthecorn
Mouldsgrowparasites,epiphytes
orsaprophytes
Homeplantassociationveryspecific
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Thedetectionandidentificationofbacteria
Page13
Thecontextofpostharvestgrain
maybedamaged
Mushroomscanpenetrate
thedamagedsectionjyvn
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Thedetectionandidentificationofbacteria
Inorderforfungalgrowthwouldbeimpeded
cerealsafterpuimisen
dried
Dryinglessthan14%watercontent
Whenthegrainwatercontentislessthan5.13%it
themicrobiologicalshelflife
Oilbearingcropseedsmustbedriedbelow
12.5%
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Ifthegrainisstoredtoo
humid
Mouldsandfungigrow
Metabolismproducesheatandtheharvestheatsup
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Thedetectionandidentificationofbacteria
Mushroomskilltheembryoandseednolongergerminate
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Ifthegrainisstoredtoo
inahumidatmosphereof6590%
itgrowsvarastohomeita
Eurotium,Aspergillus,Penicillum
Later,Mucor,Rhizopus,and
Nigraspora
Cropqualityispooriftheconcentrationofsporesoffungal
morethan10
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Thedetectionandidentificationofbacteria
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Grainstorage
Relativehumidity<85%
10C
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Ifthegraintogrowmushrooms
asaresult,cellrespirationarises
metabolicwater
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Thedetectionandidentificationofbacteria
Cerealiswettedandlesskserofyyttisetsienetkin
cangrow
Aspergilluscandidus,A.flavus,A.parasiticus,A.
fumigatus,Emerciellanidulans,Penicillum
Piceum,P.capsulatum
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Thesefungiform
mycelium
Canbindgrainklimpeiksi
orsolidlumps
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Thedetectionandidentificationofbacteria
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Growthandtemperature
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Thegrowthandactivityofthewater
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Thedetectionandidentificationofbacteria
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FAOandtheWHOrecommendation
max30gaflatoxin/kgfood
timehigh,butmalnutritionversus
livercancer
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Thedetectionandidentificationofbacteria
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AflatoxinB1
LD
dogs0.5mg/kgbodyweight
50
LD
rat9mg/kg
50
ForratsandrainbowtroutaflatoxinB1inone
themostcarcinogeniccompounds
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AflatoxinB1
India19741000peoplebecameill
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Thedetectionandidentificationofbacteria
aftereatingmoldyrice
100died
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Thedetectionandidentificationofbacteria
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OchratoxinA
WasfirstisolatedA.ochraceusSouth
Africa
Themoststudiedbarley,wherePverrucosum
toproduceit
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OchratoxinA
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Thedetectionandidentificationofbacteria
Inpigs,causedbykidneyproblems
Movestoaccompanymeat
Peoplewhoeatcannedalongtime
homekypsennettyjhamsmaybeexposed
(Balkanendemicnephropathy)
nephrotoxicandmaycauserenalcellcarcinoma
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Sterigmatokystiini
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Thedetectionandidentificationofbacteria
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Sterigamtokystiini
Aflatoksiinisynteesinprecursor
ManyofAspergillusmoldsproduceit
especiallyA.versicolor
Notastoxicandcarcinogenicthan
Aflatoxin
Itisfoundinmanyfood
ForalongtimestoredinEdamandGoudacheese
Thepoisononlyinthesurfacelayers
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Patulin
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Thedetectionandidentificationofbacteria
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Patulin
PenicilliumandAspergillusproducers,including
Byssochlamys
Apples,blueberries,strawberries,currants,
gooseberries
Theeffectsofpatulinisnotknownexactly
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Thedetectionandidentificationofbacteria
Nordiccountries,themaximumallowableamountis
50g/kg
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Patulin
Discoveredin1942,itwasthoughtthatitwouldbe
goodantibioticbecauseofitslarge
Theantimicrobialactivityof
In1959itwasassociatedwithcattlepoisoning
moldygerminatedbarleygrain
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Thedetectionandidentificationofbacteria
Patulin
Notverystable,exceptinacidic
conditionssuchasapplejuice
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Thedetectionandidentificationofbacteria
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T2
DermonecrosiscattleintheUSA
MaybeinRussia194247disease
"AlimentaryToxicAleukia"
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Thedetectionandidentificationofbacteria
T2
immunosuppressiveagentssuchasochratoxin
andaflatoxin
allthreealsoinhibit
proteinsynthesis,butatdifferentpoints
Mixturesmaybesynergistic
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DON
Deoksynivalenol(=vomitoxin)
Japan"redmouthdisease",diarrheaand
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Thedetectionandidentificationofbacteria
vomit
F.graminearum
FeedfactorrequiredforrefusalinpigsinNorthCarolina
LesstoxicthanT2
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Verrukariinit
Stachybotrysatra
Inhorses,aftereatingmoldyhay
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Thedetectionandidentificationofbacteria
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Zearalenone
vulvovaginitispigswhoatethemold
maize
infertility
fewerchicks
insomecountriesareusedasanabolics
increaseingrowthfactorsandinothersitisforbidden
monitoringtheproblem,becauseifthefeedisitso
Itisalsodetectableintheflesh
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Thedetectionandidentificationofbacteria
Ergotamine
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Ergotism
alsoreferredtoasStAnthonyfire
Thereisageneral
toxiceffectspoorlyunderstood
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Thedetectionandidentificationofbacteria
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