....

()





1)

..

..
2) Induction of inducible CD4+CD25+Foxp3+
regulatory T lymphocytes by porcine reproductive and
respiratory syndrome virus (PRRSV)
inducible
regulatory T lymphocyte (Treg)

(suppressive activity)


inducible regulatory T
lymphocyte

3) The negative impact of porcine reproductive and
respiratory syndrome virus infection on the efficacy of
classical swine fever vaccine





(vaccine failure)

(interleukin 10)

..
4) Taming PRRSV: revisiting the control
strategies and vaccine design

5) Factors critical for successful vaccination
against classical swine fever in endemic areas

(cellmediated immunity)

..
6) The kinetics of cytokine production and CD25
expression by porcine lymphocyte subpopulations following
exposure to classical swine fever virus (CSFV)
lymphocyte subpopulation


/

CD4+CD8+ (memory T cells)
interferon-gamma (IFN-)

CD4-CD8+

IFN -

IFN-

IL-2 receptor (CD25)

7)

porcine respiratory disease complex (PRDC)

..


..
8) Negative effects of porcine endemic
pathogens on the immune system

9) (US provisional) Needle-free administration
of PRRSV vaccines US provisional application, Electronic
Filing System (EFS)


5

Taming
strategy
...
(nucleocapsid)

(antigen-specific T
lymphocytes)

protective / neutralizing
epitope

(intradermal)
(needleless applicator)

transdermal applicator

US
provisional

1) The genome sequence analysis of H5N1 avian

influenza A virus isolated from the outbreak among poultry in
Thailand
H5N1 ..

H5N1

(H5N1)
..

..
2) Immunobiology of avian influenza H5N1
and implication on the control strategies

3) Serological evidence of pig-to-human influenza
virus transmission on Thai swine


7

4) Brief report: molecular characterization of a novel

reassorted pandemic H1N1 2009 in Thai pigs
reassorted pandemic H1N1 2009 virus (rH1N1)
(reassortment)
(pandemic H1N1 2009)
(swine influenza virus H1N1)

rH1N1





pH1N1

5) Pandemic (H1N1) 2009 virus on commercial
swine farm




8

6) Influenza Virus (H5N1) in Live Bird Markets and

Food Markets, Thailand
(Influenza virus H5N1)

..
..

7) Genetic characterization of H5N1 influenza A
viruses isolated from zoo tigers in Thailand

H5N1

(wave)
9

PB2 glutamic acid (E) lysine (K)

/


(H5N1)

..
..

8) Genetic characterization of influenza A viruses

(H5N1) isolated from 3rd wave of Thailand AI outbreaks
H5N1

9) Genetic characterization of 2008 reassortant

influenza A virus (H5N1), Thailand
reassortant
H5N1 subclade 1.1 subclade 1.2
10

H5N1




10) Comparative analysis of complete
nucleotide sequence of porcine reproductive and respiratory
syndrome virus (PRRSV) isolates in Thailand (US and EU
genotypes)











(... ...)

11

1. Suradhat, S., Thanawongnuwech, R., and Poovorawan, Y.

2003. Upregulation of IL-10 gene expression in porcine
peripheral blood mononuclear cells by Porcine
Reproductive and Respiratory Syndrome Virus (PRRSV). J.
Gen. Virol. 84:453-459.
2. Suradhat, S. and Thanawongnuwech, R. 2003.
Upregulation of interleukin-10 gene expression in the
leukocytes of pigs infected with porcine reproductive and
respiratory syndrome virus J. Gen. Virol. 84: 2755-2760.
3. Viseshakul, N., Thanawongnuwech, R., Amonsin, A.,
Suradhat, S., Payungporn, S., Keawchareon, J.,
Oraveerakul, K., Wongyanin, P., Plitkul, S., Theamboonlers,
A. and Poovorawan, Y. 2004. The genome sequence
analysis of H5N1 avian influenza A virus isolated from the
outbreak among poultry in Thailand. Virology. 328:169176.
4. Suradhat, S., Sada, W., Buranapraditkun, S.,
Damrongwatanapokin, S. 2005. The kinetics of cytokine
production and CD25 expression by porcine lymphocyte
subpopulations following exposure to classical swine fever
virus (CSFV). Vet. Immunol. Immunopathol. 106: 197-208.
5. Amonsin, A., Payungporn, S., Theamboonlers, A.,
Thanawongnuwech, R., Suradhat, S., Pariyothorn, N.,
Tantilertcharoen, R., Damrongwantanapokin, S.,
Buranathai, C., Chaisingh, A., Songserm, T. and
Poovorawan, Y. 2006. Genetic characterization of H5N1
influenza A viruses isolated from zoo tigers in Thailand.
Virology. 344: 480-491.
6. Suradhat, S., Kesdangsakonwut, S., Sada, W.,
Buranapraditkun, S., Wongsawang, S., and
Thanawongnuwech, R. 2006. The negative impact of
porcine reproductive and respiratory syndrome virus
12

infection on the efficacy of classical swine fever vaccine.

Vaccine. 24: 2634-2642.
7. Amonsin, A., Chutinimitkul, S., Pariyothorn, N., Songserm,
T., Damrongwantanapokin, S., Puranaveja, S., Jam-On, R.,
Sae-Heng, N., Payungporn, S., Theamboonlers, A.,
Chaisingh, A., Tantilertcharoen, R., Suradhat, S.,
Thanawongnuwech, R., and Poovorawan, Y. 2006. Genetic
characterization of influenza A viruses (H5N1) isolated
from 3rd wave of Thailand AI outbreaks. Virus Res.
122:194-199.
8. Suradhat, S., Damrongwatanapokin, S. and
Thanawongnuwech, R. 2007. Factors critical for successful
vaccination against classical swine fever in endemic areas.
Vet Microbiology.119: 1-9.
9. Suradhat, S., 2008. Negative effects of porcine endemic
pathogens on the immune system. Journal of Applied
Animal Science. January-April, 1(1):19-30.
10.
Amonsin, A., Choatrakol, C., Lapkuntod, J.,
Tantilertcharoen, R., Thanawongnuwech, R., Suradhat,S.,
Suwannakarn, K., Theamboonlers, A., and Pooworawan, Y.
2008. Influenza virus (H5N1) in live bird markets and food
markets, Thailand. Emerging Infectious Disease.
November, 2008. 14(11): 1739-42.
11.
Amonsin, A., Kedkovid, R., Puranaveja, S., Wonyanin,
P., Suradhat, S., and Thanawongnuwech, R. 2009.
Comparative analysis of complete nucleotide sequence of
porcine reproductive and respiratory syndrome virus
(PRRSV) isolates in Thailand (US and EU genotypes).
Virology Journal. 16 September. 6: 143.
12.
Suradhat, S., Wongyanin, P., Buranapraditkun, S.,
Thanawongnuwech, R. and Roth, J.A. 2009. Effect of PRRSV
infected monocyte derived-dendritic cells on the induction
of interleukin-10 producing lymphocytes. The 2009
International PRRS Symposium: The PRRS Coordinated
Agricultural Project (CAP), National Pork Board and NC-229
Respiratory Diseases Committee. 4-5 December, Chicago
USA.
13

13.
Suradhat, S., Wongyanin, P., Buranapraditkun, S.,
Thanawongnuwech, R. and Roth, J.A.2009. Effect of
nucleocapsid protein of PRRSV on the induction of
interleukin-10 producing lymphocytes. The 2009
International PRRS Symposium: The PRRS Coordinated
Agricultural Project (CAP), National Pork Board and NC-229
Respiratory Diseases Committee. 4-5 December, Chicago
USA.
14.
Thanawongnuwech,R., and Suradhat, S. 2010. Taming
PRRSV: Revisiting the control strategies and vaccine
design. Virus Research. 154: 133-140.
15.
Wongyanin, P., Buranapraditkun, S., Chokesshaiusaha, K., Thanawonguwech, R., and Suradhat, S. 2010.
Induction of inducible CD4+ CD25+ Foxp3+ regulatory T
lymphocytes by porcine reproductive and respiratory
syndrome virus (PRRSV) Veterinary Immunology and
Immunopathology. 133: 170-182.
16.
Bunpapong N, Nonthabenjawan N, Chaiwong S,
Tangwangvivat R, Boonyapisitsopa S, Jairak W, Tuanudom
R, Prakairungnamthip D, Suradhat S, Thanawongnuwech
R, Amonsin A. 2014.
Genetic characterization of canine influenza A virus
(H3N2) in Thailand. Virus Genes. 48(1):56-63.
17.
Wongyanin P, Buranapraditkul S, Yoo D,
Thanawongnuwech R, Roth JA, Suradhat S. 2012.
Role of porcine reproductive and respiratory syndrome
virus nucleocapsid protein in induction of interleukin-10
and regulatory T-lymphocytes (Treg). J Gen Virol. 93(Pt
6):1236-46.
18.
Kitikoon P, Sreta D, Na Ayudhya SN,
Wongphatcharachai M, Lapkuntod J, Prakairungnamthip D,
Bunpapong N, Suradhat S, Thanawongnuwech R, Amonsin
A. 2011. Brief report: Molecular characterization of a novel
reassorted pandemic H1N1 2009 in Thai pigs. Virus Genes.
43(1):1-5.
19.
Kitikoon P, Sreta D, Tuanudom R, Amonsin A,
Suradhat S, Oraveerakul K, Poovorawan Y,
Thanawongnuwech R. 2011. Serological evidence of pig14

to-human influenza virus transmission on Thai swine

farms. Vet Microbiol. 24;148(2-4)

15