Food Science

Bacteria Lab Report

Date: 19/01/15
Name: Preme, Ravisara Karnjanolarn
Earn, Pemika Sritong-in
Putter, Yanaj Budhijalananda
Non, Tanarut Lerdsuwanrut
Introduction:
The human microbiome is the community of microorganisms that
mostly live in our large intestines. These are the gut bacteria that have many
useful function to supporting life, they also perform an important role in our
digestive system. They digest carbohydrates, prevent diseases, synthesize
and absorb nutrients/minerals such as Vitamin K and Vitamin B.
These bacteria can typically found in many common kind of foods
which are Fermented Products such as Yogurt, Kimchi, and Miso soup. The
preparation process is called Fermentation- the conversion of carbohydrates
to alcohol or acids. For the example, the process of making Yogurt starts by
adding some bacterias (Streptococcus thermophilus and Lactobacillus
bulgaricus) to the milk.

Purpose:
To find the bacterial in many different substances that which one have
the most bacteria and we used three example which are Betagen, Bio (Yogurt
mixed with water), and Water in this experiment.
Hypothesis:
If the Betagen, Bio (Yogurt mixed with water), and Water contain
beneficial bacteria, some of bacteria will appear in agar plates after we did
the experiment for a few days.
Material:
1) Lab coat, gloves, and goggles
2) 3 Agar Plates
3) Anaerobic Environment
4) Medicine Dropper
5) Glass Spreader
6) Alcohol
7) Sterilize Equipment
-Anti-Bacterial paper towel
-Alcohol Burner
8) Substances for the experiment
-Betagen
-Bio (Yogurt mixed with water)
-Water

Experimental Procedures:
1. Put on lab coat, gloves, and goggles.
2. Obtain medicine dropper, glass spreader, 3 agar plates

3. Moisten a paper towel with 70% isopropyl rubbing alcohol to prevent

bacterial contamination
4. Place your 3 agar plates on the paper towel. Do not open them.
5. Using a marker, label the bottom of each agar plate 1-3
6. Suck up rubbing alcohol into the medicine dropper. Hold for 10
seconds. Squirt it out onto a paper towel. Use the paper towel to rub the
outside of the dropper.
7. Hold the dropper over the lamp to burn off the alcohol
8. Set the dropper down but propped up onto a paper towel so that the
tip of the dropper does not touch anything.
9. Obtain the diluted yogurt (yogurt mixed with water)
10. Using the sterilized dropper suck up yogurt to fill the dropper. Do
not touch anything other than the yogurt. Continue to hold the dropper in
one hand
11. Open 2 agar plates and add a few drops of the yogurt to the plates,
one at a time. Add the drops into the center of the plates, one drop on top of
the other.
12. Do not set the lids to the agar plates down on anything
13. Put the lids back onto the agar plates
14. Onto the 3rd agar plate, add a few drops of distilled water, for the
control. (either with new dropper or teacher will provide drops for you)
15. Dip the glass spreader into rubbing alcohol for 10 seconds. Hold it
over the lamp to burn off the alcohol.
16. Do not let the triangular part of the glass spreader touch anything.
Hold it in your hand the whole time.
17. Open each agar plate (do not put the lids down) and use the glass
spreader to spread the diluted yogurt in a specific pattern. Start with agar
plate 3 to prevent contamination on the control with diluted yogurt. Do not
push too hard so that you break the agar.
18. After you finish each plate, quickly put its lid back on. Wipe the
glass spreader clean between each plate.
19. Let the plates sit for a minute to help the yogurt attach to the agar.
20. The lab instructor will prepare an anaerobic chamber to place all of
the groups agar plates into. The anaerobic chamber consists of a plastic
container and a candle. When the candle is lit, place the lid onto the
container and cover the outside with clay. The candle will eventually go out,
leaving a container with no oxygen.
21. Wait a few days, then check on your agar plates.
Results and Analyzing Results:

After we did an experiment we found out that our control, water is

growing bacteria with a big spot. Which make us confuse at first because, we
thought water must not growing bacteria. Its take us about one day to know
that the bacteria in our control plate is a bacteria that we do not expect to
have in the experiment. The reason that can cause this is the equipment that
we use are not clean when we use it. The second reason that can cause is
when we are talking or breathing across the agar plate when we do the
experiment. This can cause bacteria in our body get into the plate and start
to grow in the
plate.

In the picture you can see the sample of Bio yogurt also growing good
bacteria but not same type with the control. This is the type of bacteria that
we expect to grow in the experiment. It is smaller and much more spread
than the bacteria that grow in our control plate. The Bio yogurt is another
choice for you if you want to eat yogurt that good for your health because
good bacteria that
help your body
can grow in Bio
successfully.

If you compare Betagen sample with those two above which are water
and bio yogurt samples, you might think why betagen didnt grow any
bacteria at all but actually Betagen also grow small size of bacteria. We saw
small size of bacteria in the lab but unfortunately we can not see in the
picture. It is too small, so we can tell you that all of it( Water, Bio yogurt, and
Betagen) are growing bacteria.

Conclusion:
In this experiment we had to grow a bacteria on the agar plates. We
need to make all of the equipments clean as possible to do this experiment.
It was also very important to make our agar plates to be anaerobic

environment. The Bio plate had a bacteria that we expected to have in bio
plate. On another hand, in our control plate had another type of bacteria that
was not in the experiment. It can cause by many causes. For example,
unclean equipments, or it can cause when we talk to each other across the
plate.In our experiment, nothing happen on our betagen plate. We can
improve this experiment by take care about neat and clean.