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    Deni Saputra
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    WALNE'S MEDIUM FOR ALGAL CULTURES Recommended for large volumes of aquaculture strains. Contains Trace metal solution (TMS) and vitamin solution Vitamin B12. (Cyanocobalamin) Vitamin B1 (Thiamine.HCl) Make up to 100 ml with distilled water.

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    WALNE'S MEDIUM FOR ALGAL CULTURES Recommended for large volumes of aquaculture strains. Contains Trace metal solution (TMS) and vitamin solution Vitamin B12. (Cyanocobalamin) Vitamin B1 (Thiamine.HCl) Make up to 100 ml with distilled water.

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    11 views1 page

    Walnes

    Uploaded by

    Deni Saputra
    WALNE'S MEDIUM FOR ALGAL CULTURES Recommended for large volumes of aquaculture strains. Contains Trace metal solution (TMS) and vitamin solution Vitamin B12. (Cyanocobalamin) Vitamin B1 (Thiamine.HCl) Make up to 100 ml with distilled water.

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    of 1

    WALNE'SMEDIUMFORALGALCULTURES

    Recommendedforlargevolumesofaquaculturestrains

    Stocks

    per100ml
    (1) Tracemetalsolution(TMS)
    ZnCl2
    2.1g
    CoCl2.6H2O
    2.0g
    (NH4)6Mo7O24.4H2O
    0.9g
    CuSO4.5H2O
    2.0g
    Make up to 100 ml with distilled water. This solution is normally
    cloudy.Acidifywithafewdropsofconc.HCltogiveaclearsolution.
    (2) Vitaminsolution
    VitaminB12.(Cyanocobalamin)
    VitaminB1 (Thiamine.HCl)
    VitaminH(Biotin)
    Makeupto100mlwithdistilledwater.

    10.0mg
    10.0mg
    200.0g
    perlitre

    (3) Nutrientsolution
    FeCl3.6H20
    MnCl2.4H20
    H3BO3
    EDTA(Disodiumsalt)
    NaH2PO4.2H2O
    NaNO3
    TMS(1above)
    Makeupto1litrewithdistilledwater.

    Medium
    Nutrientsolution(3)
    Vitaminsolution(2)
    Sterilisedseawater

    1.3g
    0.36g
    33.6g
    45.0g
    20.0g
    100.0g
    1.0ml

    perlitre
    1.0ml
    0.1ml
    1.0litre

    mediarecipes

    Dispensenutrientandvitaminsolutionsseparatelyinto10mland1mlrespectively
    and autoclave at 15 psi for 15 minutes. Addan aliquot of each aseptically to 10
    litresofsterilisedseawater.

    Reference
    WalnePR(1970)Studiesonthefoodvalueofnineteengeneraofalgaetojuvenile
    bivalves of the genera Ostrea, Crassostrea, Mercenaria, and Mytilis. Fish. Invest.
    26,162.

    7August2002

    CCAP(CultureCollectionofAlgaeandProtozoa),DunstaffnageMarineLaboratory,
    Oban,Argyll,PA371QA,UK
    Tel:+44(0)1631559000Fax:+44(0)1631559001Email:ccap@sams.ac.ukWeb:www.ccap.ac.uk

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