Tutorial Questions for Genetic engineering Technology

Q1. What are vectors? Explain. Q2. Which are the different types of vectors in detail? Q3. Explain the structure of pBR 322 and pUC 18 vector? Q4. Explain the structure of phagemid and cosmid vector? Q5. Explain Lambda vectors and artificial chromosomes in detail? Q6. What are expression vectors and enlist their functions? Q7. What is promoter fusion and how is the expression of cloned genes checked? Q8. Explain the construction of a genomic library? Q9. Explain the construction of a cDNA library? Q10. What are DNA probes? Q11. Which are the various hybridization techniques? Q12. Which are the various enzyme used in genetic engineering? Q13. Explain the function of reverse transcriptase in cDNA library synthesis? Q14. Explain the function of Bal 31 nuclease enzyme? Q15. Explain direct identification of a gene from the expressed clone? Q16. Explain the identification of a gene from gene library? Q17. Explain the sequencing of DNA by Sangers Method? Q18. Explain the sequencing of DNA by Maxim Gilbert Method? Q19. Explain northern Southern and Western blotting techniques in detail? Q20. Explain the working of Automated sequencers in detail?