DEPARTMENT OF BIOTECHNOLOGY

STUDY OF DIFFERENT PHYSICAL PROPERTIES OF WATER Requirements: - Waste water sample, pH meter, Conductivity meter, Turbidity meter and Glass beaker. CHEMICAL OXYGEN DEMAND

Requirements:-Reflux Flask, Glass Beads, Condenser, Titration assembly. Reagents: - 1. Potassium Dichromate (K2Cr2O7 - 0.025N) 2. Concentrated H2SO4 3. Mercuric Sulfate 4. Silver Sulfate 5. Ferrous Ammonium Sulfate

TOTAL SOLIDS

Requirements:- Whatman Filter paper no.42, oven, Evaporating Dish, Funnel, Conical Flask, Measuring Cylinder, Heating Mantle, Desiccators, Hot air oven.

BIOLOGICAL OXYGEN DEMAND Requirements: - BOD Bottles, Pipettes, BOD Incubator Reagents: - Potassium Dihydrogen Phosphate (KH2PO4). Magnesium Sulfate (MgSO4.7H2O). Di - Potassium Hydrogen Phosphate (K2HPO4). Calcium Chloride (CaCl2). Ferric Chloride (FeCl3.6H2O). Ammonium Chloride (NH4Cl). 0.025N Sodium Thiosulfate (Na2SO3). Alkaline K1 Solution. Magnesium Sulfate. Starch Solution. Concentrated H2SO4. TOTAL NITROGEN ESTIMATION Requirements: - Kjeldhal digestion apparatus Reagents Cupric sulfate 2. Conc. H2SO4 3. Potassium sulfate K2SO4

SIES GRADUATE SCHOOL OF TECHNOLOGY

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DEPARTMENT OF BIOTECHNOLOGY
4. Mixed indicator (Methyl red + Methyl blue + Boric acid powder) 5. Std H2SO4 solution.

PRESENCE OF PHOSPHATES IN WATER SAMPLES

Requirements: - Kjeldhal setup, Test tubes and spectrophotometer. Reagents: - Conc. Sulphuric Acid (H2SO4) 2. Phenophatelin Indicator 3. Conc. Nitric Acid (HNO3) 4. Sodium Hydroxide 1N MIXED LIQUOR VOLATILE SUSPENDED SOLIDS (MLVSS) Requirements: - Muffle furnace, Filtration apparatus, Vacuum pump, hot air oven, analytical balance, Glass fiber filter disc, desiccators.

TOTAL COLIFORM COUNT Requirements: - Test tubes (15 no.), Durhams tube 15, sterile pipette, Mac.Conkeys Broth, MPN Table. DEMONSTRATRION AND CALIBRATION OF AIR SAMPLER Requirements: - High Volume Sampler, Different parts of orifice calibrator: 1. Orifice meter 2. Bottom Plate 3. Support plate 4. Resistance Plate 5. Gasket 6. Monometer assembly

SIES GRADUATE SCHOOL OF TECHNOLOGY

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