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  • Trainno Cars 'Start Station' 'End Station' Panvel Wadala

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    Vamsi K Kuchibhotla
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    Yeast cells are treated with zymolase to break the hard outer wall and produce spheroplasts, which are washed in sorbitol buffer. Nuclei are removed by low speed centrifugation, while the cytoplasm-containing supernatant is centrifuged in a fixed-angle rotor at 6500 g.

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    Train No

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    Yeast cells are treated with zymolase to break the hard outer wall and produce spheroplasts, which are washed in sorbitol buffer. Nuclei are removed by low speed centrifugation, while the cytoplasm-containing supernatant is centrifuged in a fixed-angle rotor at 6500 g.

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    7 views1 page

    Trainno Cars 'Start Station' 'End Station' Panvel Wadala

    Uploaded by

    Vamsi K Kuchibhotla
    Yeast cells are treated with zymolase to break the hard outer wall and produce spheroplasts, which are washed in sorbitol buffer. Nuclei are removed by low speed centrifugation, while the cytoplasm-containing supernatant is centrifuged in a fixed-angle rotor at 6500 g.

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    of 1

    'Start TrainNo Cars Station'

    Panvel [6:05:00 H9 H23 H10 H11 H13 H15 H16 H18 H20 H22 9 9 9 9 9 9 9 9 9 9 AM] Panvel [6:12:00 AM] Panvel [6:23:00 AM] Panvel [6:35:00 AM] Panvel [6:51:00 AM] Panvel [7:08:00 AM] Panvel [7:20:00 AM] Panvel [7:32:00 AM] Panvel [7:48:00 AM] Panvel [8:00:00 AM]

    'End Station'
    AM] AM] AM] AM] AM] AM] AM] AM] AM] AM]

    Panvel Wadala
    7:04:00 AM 7:11:00 AM 7:22:00 AM 7:34:00 AM 7:50:00 AM 8:07:00 AM 8:19:00 AM 8:31:00 AM 8:47:00 AM 8:59:00 AM AM AM AM AM AM AM AM AM AM AM

    Mumbai CST [7:22:00 6:05:00 Mumbai CST [7:29:00 6:12:00 Mumbai CST [7:40:00 6:23:00 Mumbai CST [7:52:00 6:35:00 Mumbai CST [8:08:00 6:51:00 Mumbai CST [8:25:00 7:08:00 Mumbai CST [8:37:00 7:20:00 Mumbai CST [8:49:00 7:32:00 Mumbai CST [9:05:00 7:48:00 Mumbai CST [9:17:00 8:00:00

    1. Cell pellets, grown in monolayer or in suspension, are homogenized in homogenization buffer containing MgCl2 and KCl. 2. Sucrose is added up to 0.25 M and the nuclei are pelleted by low speed centrifugation (1000 g). 3. A second centrifugation of the supernatant at 5000 g will sediment the mitochondria. 4. The pellet is resuspended in a medium containing sucrose and Mg2+ and is subjected to few gentle strokes in a homogenizer. 5. A last centrifugation step at 5000 g will enrich mitochondria which can be resuspended in Tris buffer containing 0.25 M sucrose or in the buffer of preference for subsequent analyses. 6. Yeast cells are treated with zymolase to break the hard outer wall and produce spheroplasts, which are washed in sorbitol buffer. 7. The pellet is resuspended in homogenization buffer containing 0.6 M mannitol and cells are lysed by few strokes in a homogenizer. 8. Nuclei are removed by low speed centrifugation, while the cytoplasm-containing supernatant is centrifuged in a fixed-angle rotor at 6500 g to pellet mitochondria.

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