Bio Chemical Testing Results 2012

ABRI, CEMB, NIGAB& NIBGE

BIOTECH TEST REPORT OF CANDIDATE COTTON VARIETIRS FOR NCVT-2012 FROM

AGRICULTURAL BIOTECHNOLOGY RESEARCH INSTITUTE (ABRI), Faisalabad

TABLE1
Code

Detailed results regarding purity test based on presence of cry1A/ cry2a/ RR

determine through strip test using five plants/ entry are detailed below.
Variety

V1 HBCSD134
V2 HBCSB814
V3 HSP3
V4 KissanEarly
V5 HSP1
V6 SilverWhite
V7 HSP2
V8 SilverGold
V9 AA904
V10 RS1
V11 Auriga213
V12 Tarzan3
V13 Sayban201
V14 A011
V15 TARZAN2
V16 Sitara10M
V17 SB149
V18 Sitara12
V19 Sun1
V20 Sitara11M
V21 KZ389
V22 JS1
V23 Leader1
V24 BGC09
V25 Sayban202
V26 Silkee
V27 RCA2
V28 NS161
V29 AGC777
V30 AA919
V31 RCA1
V32 BS52
V33 Trend1
V34 A555
V35 NIABBt1
V36 RH627
V37 FH118
V38 GH142

Cry1A
100
80
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
60
100
60
80
100
100
40
100
100
100
100
100
60
100
100
100
100
60
100
60

NT:NotTested

Purity(%)
Cry2A
100
40
0
100
0
100
0
100
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0

Code
RR
100
0
NT
0
NT
0
NT
100
0
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT

V39
V40
V41
V42
V43
V44
V45
V46
V47
V48
V49
V50
V51
V52
V53
V54
V55
V56
V57
V58
V59
V60
V61
V62
V63
V64
V65
V66
V67
V68
V69
V70
V71
V72
V73
V74
V75

Variety
FH142
CEMB33
MNH456
VH303
BZU75
VH282
CRIS508
MM58
IRNIBGE5
SLH4
IUB222
IRNIBGE4
CIM600
BH180
CIM602
CEMB55
CIM599
CEMB44
BtBH178
GS444
DNH105
CIM612
PB38
IUB11
MPSII
NIAB112
Cyto124
JS212
VH300
CRIS510
NIA80
CIM591
BH176
CIM573(Standard)
CRIS342(Standard)
CIM598(standard)
MNH886(Standard)

Purity(%)
Cry1A
Cry2A
100
0
100
0
100
0
80
0
0
0
60
0
100
0
100
0
100
0
100
0
100
0
100
0
100
0
100
0
100
0
40
0
100
0
40
0
100
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
40
0
0
0
0
0
0
0
0
0
40
0
0
0
0
0
100
0
100
0

RR
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT

TABLE2
Code

V1
V2
V3
V4
V5
V6
V7
V8
V9
V10
V11
V12
V13
V14
V15
V16
V17
V18
V19
V20
V21
V22
V23
V24
V25
V26
V27
V28
V29
V30
V31
V32
V33
V34
V35
V36
V37
V38

Results obtained from event specific PCR for MON531 (Cry1A), MON 15985
(Cry2A) and MON 1445 (Roundup ready) are detailed below.
Variety

HBCSD134
HBCSB814
HSP3
KissanEarly
HSP1
SilverWhite
HSP2
SilverGold
AA904
RS1
Auriga213
Tarzan3
Sayban201
A011
TARZAN2
Sitara10M
SB149
Sitara12
Sun1
Sitara11M
KZ389
JS1
Leader1
BGC09
Sayban202
Silkee
RCA2
NS161
AGC777
AA919
RCA1
BS52
Trend1
A555
NIABBt1
RH627
FH118
GH142

NT:NotTested

EventDetection
MON531 MON15985 MON1445
(Cry1A) (Cry2A)
(RR)
+
+
+
+

NT
+
NT
NT
+
+
NT
+
NT
NT
+
+
NT
+
NT
NT
+
+
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT

Code

Variety

V39
V40
V41
V42
V43
V44
V45
V46
V47
V48
V49
V50
V51
V52
V53
V54
V55
V56
V57
V58
V59
V60
V61
V62
V63
V64
V65
V66
V67
V68
V69
V70
V71
V72
V73
V74
V75

FH142
CEMB33
MNH456
VH303
BZU75
VH282
CRIS508
MM58
IRNIBGE5
SLH4
IUB222
IRNIBGE4
CIM600
BH180
CIM602
CEMB55
CIM599
CEMB44
BtBH178
GS444
DNH105
CIM612
PB38
IUB11
MPSII
NIAB112
Cyto124
JS212
VH300
CRIS510
NIA80
CIM591
BH176
CIM573(Std)
CRIS342(Std)
CIM598(std)
MNH886(Std)

EventDetection
MON531 MON15985 MON1445
(Cry1A) (Cry2A)
(RR)
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
NT
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
+
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
+
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
+
NT
NT
NT
NT
NT
NT
NT
NT
+
NT
NT
+
NT
NT

TABLE3

Results on quantification of Cry1Ac are described below.

Code

Variety

V1
V2
V3
V4
V5
V6
V7
V8
V9
V10
V11
V12
V13
V14
V15
V16
V17
V18
V19
V20
V21
V22
V23
V24
V25
V26
V27
V28
V29
V30
V31
V32
V33
V34
V35
V36
V37
V38

HBCSD134
HBCSB814
HSP3
KissanEarly
HSP1
SilverWhite
HSP2
SilverGold
AA904
RS1
Auriga213
Tarzan3
Sayban201
A011
TARZAN2
Sitara10M
SB149
Sitara12
Sun1
Sitara11M
KZ389
JS1
Leader1
BGC09
Sayban202
Silkee
RCA2
NS161
AGC777
AA919
RCA1
BS52
Trend1
A555
NIABBt1
RH627
FH118
GH142

Cry1Ac80daysaftersowing
(g/gofthefreshweight)
6.19
4.67
3.85
2.62
3.50
3.74
3.11
1.72
5.73
5.03
4.56
4.87
6.13
3.10
7.45
1.97
3.32
0.99
2.62
0.01
5.11
4.85
4.34
5.20
0.65
0.67
0.03
3.18
2.06
0.43
3.50
0.90
4.80
2.33
0.50
2.53
1.06
0.80

S.No.
V39
V40
V41
V42
V43
V44
V45
V46
V47
V48
V49
V50
V51
V52
V53
V54
V55
V56
V57
V58
V59
V60
V61
V62
V63
V64
V65
V66
V67
V68
V69
V70
V71
V72
V73
V74
V75

Variety
FH142
CEMB33
MNH456
VH303
BZU75
VH282
CRIS508
MM58
IRNIBGE5
SLH4
IUB222
IRNIBGE4
CIM600
BH180
CIM602
CEMB55
CIM599
CEMB44
BtBH178
GS444
DNH105
CIM612
PB38
IUB11
MPSII
NIAB112
Cyto124
JS212
VH300
CRIS510
NIA80
CIM591
BH176
CIM573(Standard)
CRIS342(Standard)
CIM598(standard)
MNH886(Standard)

Cry1Ac80daysaftersowing
(g/gofthefreshweight)
2.50
1.25
1.08
5.00
NT
0.98
0.97
2.58
1.15
4.35
5.03
4.35
3.28
0.45
3.53
4.99
2.42
1.55
2.47
NT
NT
NT
NT
NT
NT
NT
NT
3.30
NT
NT
NT
NT
3.21
NT
NT
0.38
1.64

DNAANDPROTEIN BASED DETECTIONOFBTGENE(CRY1ACANDCRY2A)INCOTTONVARIETIESAT

NATIONALCENTREOFEXCELLENCEINMOLECULARBIOLOGY(CEMB),Lahore

GMODetectionofBtCotton
S.
No.
V1
V2
V3
V4
V5
V6
V7
V8
V9
V10
V11
V12
V13
V14
V15
V16
V17
V18
V19
V20
V21
V22
V23
V24
V25
V26
V27
V28
V29
V30
V31
V32
V33
V34
V35
V36
V37
V38
V39
V40
V41
V42

SampleNo.

HBCSD134
HBCSB814
HSP3
KissanEarly
HSP1
SilverWhite
HSP2
SilverGold
AA904
RS1
Auriga213
Tarzan3
Sayban201
A011
TARZAN2
Sitara10M
SB149
Sitara12
Sun1
Sitara11M
KZ389
JS1
Leader1
BGC09
Sayban202
Silkee
RCA2
NS161
AGC777
AA919
RCA1
BS52
Trend1
A555
NIABBt1
RH627
FH118
GH142
FH142
CEMB33
MNH456
VH303

PCR

ELISA

Cry1Ac

Cry2A

Cry1Ac
g/g

Cry2A
g/g

+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve

NC
NC
NC
NC
NC
NC
NC
NC
+ve
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
+ve
NC
NC
NC
NC
NC
NC
NC
NC
NC
+ve
NC
NC

0.352
0.354
0.343
0.615
0.646
0.652
0.567
0.582
0.691
0.584
0.615
0.567
0.535
0.352
0.354
0.343
0.535
0.573
0.612
0.553
0.571
0.683
0.332
0.595
0.612
0.593
0.323
0.346
0.451
0.643
0.621
0.362
0.682
0.572
0.675
0.496
0.332
0.512
0.625
0.673
0.632
0.628

NC
NC
NC
NC
NC
NC
NC
NC
0.365
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
0.543
NC
NC
NC
NC
NC
NC
NC
NC
NC
0.632
NC
NC

V43
V44
V45
V46
V47
V48
V49
V50
V51
V52
V53
V54
V55
V56
V57
V58
V59
V60
V61
V62
V63
V64
V65
V66
V67
V68
V69
V70
V71
V72
V73
V74
V75

BZU75
VH282
CRIS508
MM58
IRNIBGE5
SLH4
IUB222
IRNIBGE4
CIM600
BH180
CIM602
CEMB55
CIM599
CEMB44
BtBH178
GS444
DNH105
CIM612
PB38
IUB11
MPSII
NIAB112
Cyto124
JS212
VH300
CRIS510
NIA80
CIM591
BH176

+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve

CIM573(Standard)
CRIS342(Standard)
CIM598(standard)
MNH886(Standard)

NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
+ve
NC
+ve
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC

0.621
0.559
0.650
0.582
0.671
0.576
0.346
0.451
0.671
0.576
0.683
0.652
0.595
0.612
0.562
0.516
0.463
0.524
0.489
0.561
0.513
0.646
0.652
0.567
0.582
0.691
0.584
0.615
0.567
0.535
0.573
0.612
0.553

NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
0.468
NC
0.673
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC

RESULTS OF NATIONAL BT COTTON TRIAL-2012 CONDUCTED AT

NATIONAL INSTITUTE FOR GENOMICS & ADVANCED BIOTECHNOLOGY

(NIGAB), NARC-ISLAMABAD
TABLE1:QuantitativeELISAforquantificationofCry1Actoxinlevel.
S.
No.
V1
V2
V3
V4
V5
V6
V7
V8
V9
V10
V11
V12
V13
V14
V15
V16
V17
V18
V19
V20
V21
V22
V23
V24
V25
V26
V27
V28
V29
V30
V31
V32
V33
V34
V35
V36
V37
V38

Variety
HBCSD134
HBCSB814
HSP3
KissanEarly
HSP1
SilverWhite
HSP2
SilverGold
AA904
RS1
Auriga213
Tarzan3
Sayban201
A011
TARZAN2
Sitara10M
SB149
Sitara12
Sun1
Sitara11M
KZ389
JS1
Leader1
BGC09
Sayban202
Silkee
RCA2
NS161
AGC777
AA919
RCA1
BS52
Trend1
A555
NIABBt1
RH627
FH118
GH142

80*DASg/gfreshleaf
weight
0.633138686
0.672189781
0.516532847
0.477664234
0.567080292
0.475225487
0.542992701
0.579124088
0.800109489
0.702822229
0.75959854
0.318375948
0.332043796
0.530433518
0.786240876
0.461970803
0.822737226
0.455352627
0.729971303
0.389603821
0.621094891
0.861681699
0.412883212
0.697007299
0.676934307
0.462616339
0.71700611
0.533534701
0.583740876
0.286765945
0.08179938
0.444270073
0.355766423
0.305218978
0.392554292
0.419270073
0.647554745
0.414708029

S.No.
V39
V40
V41
V42
V43
V44
V45
V46
V47
V48
V49
V50
V51
V52
V53
V54
V55
V56
V57
V58
V59
V60
V61
V62
V63
V64
V65
V66
V67
V68
V69
V70
V71
V72
V73
V74
V75

Variety
FH142
CEMB33
MNH456
VH303
BZU75
VH282
CRIS508
MM58
IRNIBGE5
SLH4
IUB222
IRNIBGE4
CIM600
BH180
CIM602
CEMB55
CIM599
CEMB44
BtBH178
GS444
DNH105
CIM612
PB38
IUB11
MPSII
NIAB112
Cyto124
JS212
VH300
CRIS510
NIA80
CIM591
BH176
CIM573(Standard)
CRIS342(Standard)
CIM598(standard)
MNH886(Standard)

80*DASg/gfreshleafweight
0.609820174
0.627311042
0.367810219
0.60229927
0.499744526
0.616350365
0.725302051
0.45850365
0.385145985
0.813430657
0.330036496
0.392554088
0.63879562
0.476934307
0.507773723
0.626569343
0.224744526
0.385328467
0.242627737
0.067580145
NEGETIVE
0.095687036
0.049014599
0.761085249
0.07065755
0.067063082
0.720182482
0.583868613
0.568722628
0.609051095
0.773086994
0.070941055
NEGETIVE
NEGETIVE
0.093661377
0.280578993
0.587169043

*DASDaysAfterSowing.Tabulatedvaluesarethemeanofthreeplantstestedforeachentry.

Results revealed that Cry1Ac expression in all the tested lines was found to be lower than the expression level
recommended by USDA (1.5 g/g fresh leaf weight) is alarming as it will result in developing resistance
for Cry1Ac in the target insect, thus may deprive the farmer of the benefit of Bt technology.

TABLE2:Puritytestfordifferenttransgenes.
Code

Variety

V1
V2
V3
V4
V5
V6
V7
V8
V9
V10
V11
V12
V13
V14
V15
V16
V17
V18
V19
V20
V21
V22
V23
V24
V25
V26
V27
V28
V29
V30
V31
V32
V33
V34
V35
V36
V37
V38

HBCSD134
HBCSB814
HSP3
KissanEarly
HSP1
SilverWhite
HSP2
SilverGold
AA904
RS1
Auriga213
Tarzan3
Sayban201
A011
TARZAN2
Sitara10M
SB149
Sitara12
Sun1
Sitara11M
KZ389
JS1
Leader1
BGC09
Sayban202
Silkee
RCA2
NS161
AGC777
AA919
RCA1
BS52
Trend1
A555
NIABBt1
RH627
FH118
GH142

Cry1Ac/Ab Cry2A Cry1F CP4 Code

YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES

No
No
No
YES
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No

No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No

Note:Threeplantsweretestedforeachentry.

No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No

V39
V40
V41
V42
V43
V44
V45
V46
V47
V48
V49
V50
V51
V52
V53
V54
V55
V56
V57
V58
V59
V60
V61
V62
V63
V64
V65
V66
V67
V68
V69
V70
V71
V72
V73
V74
V75

Variety
FH142
CEMB33
MNH456
VH303
BZU75
VH282
CRIS508
MM58
IRNIBGE5
SLH4
IUB222
IRNIBGE4
CIM600
BH180
CIM602
CEMB55
CIM599
CEMB44
BtBH178
GS444
DNH105
CIM612
PB38
IUB11
MPSII
NIAB112
Cyto124
JS212
VH300
CRIS510
NIA80
CIM591
BH176
CIM573(Standard)
CRIS342(Standard)
CIM598(standard)
MNH886(Standard)

Cry1Ac Cry2ACry1F CP4

YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
No
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
No
No
YES
YES
YES

No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No

No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No

No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No

TABLE3:Very low level of Bt toxin (0.049-0.093 g/g)

Code
V31
V58
V60
V61
V63
V64
V70
V73

Variety
RCA1
GS444
CIM612
PB38
MPSII
NIAB112
CIM591
CRIS342(Standard)

toxing/g
0.081799
0.06758
0.095687
0.049015
0.070658
0.067063
0.070941
0.093661

TABLE4:LowlevelofBttoxin(0.2240.392g/g)
Code
V12
V13
V20
V30
V33
V34
V35
V41
V47
V49
V50
V55
V56
V57
V74

Variety
Tarzan3
Sayban201
Sitara11M
AA919
Trend1
A555
NIABBt1
MNH456
IRNIBGE5
IUB222
IRNIBGE4
CIM599
CEMB44
BtBH178
CIM598(standard)

toxing/g
0.318376
0.332044
0.389604
0.286766
0.355766
0.305219
0.392554
0.36781
0.385146
0.330036
0.392554
0.224745
0.385328
0.242628
0.280579

TABLE5:MediumlevelofBttoxin(0.4120.676g/g)
Code
V1
V2
V3
V4
V5
V6
V7
V8
V14
V16
V18
V21
V23
V24

Variety
HBCSD134
HBCSB814
HSP3
KissanEarly
HSP1
SilverWhite
HSP2
SilverGold
A011
Sitara10M
Sitara12
KZ389
Leader1
BGC09

toxing/g
0.633139
0.67219
0.516533
0.477664
0.56708
0.475225
0.542993
0.579124
0.530434
0.461971
0.455353
0.621095
0.412883
0.697007

V25
V26
V28
V29
V32
V36
V37
V38
V39
V40
V42
V43
V44
V46
V51
V52
V53
V54
V66
V67
V68
V75

Sayban202
Silkee
NS161
AGC777
BS52
RH627
FH118
GH142
FH142
CEMB33
VH303
BZU75
VH282
MM58
CIM600
BH180
CIM602
CEMB55
JS212
VH300
CRIS510
MNH886(Standard)

0.676934
0.462616
0.533535
0.583741
0.44427
0.41927
0.647555
0.414708
0.60982
0.627311
0.602299
0.499745
0.61635
0.458504
0.638796
0.476934
0.507774
0.626569
0.583869
0.568723
0.509051
0.587169

TABLE6:HighlevelofBttoxin(0.7020.86g/g)
Code
V9
V10
V11
V15
V17
V19
V22
V27
V45
V48
V62
V65
V69

Variety
AA904
RS1
Auriga213
TARZAN2
SB149
Sun1
JS1
RCA2
CRIS508
SLH4
IUB11
Cyto124
NIA80

toxing/g
0.800109
0.702822
0.759599
0.786241
0.822737
0.729971
0.861682
0.717006
0.725302
0.813431
0.761085
0.720182
0.773087

TESTINGOFBtCOTTONLINESOFNCVT2012AT
NATIONAL INSTITUTE FOR BIOTECHNOLOGY & GENETIC ENGINEERING (NIBGE)
Sr. Cotton
No Lines
(Code)
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.

V1
V2
V3
V4
V5
V6
V7
V8
V9
V10
V11
V12
V13
V14
V15
V16
V17
V18
V19
V20
V21
V22
V23
V24
V25
V26
V27
V28
V29
V30
V31
V32
V33
V34
V35
V36
V37
V38
V39
V40
V41
V42

Variety
Sad1
HBCSD134
HBCSB814
HSP3
KissanEarly
HSP1
SilverWhite
HSP2
SilverGold
AA904
RS1
Auriga213
Tarzan3
Sayban201
A011
TARZAN2
Sitara10M
SB149
Sitara12
Sun1
Sitara11M
KZ389
JS1
Leader1
BGC09
Sayban202
Silkee
RCA2
NS161
AGC777
AA919*
RCA1
BS52
Trend1
A555
NIABBt1
RH627
FH118
GH142
FH142
CEMB33
MNH456
VH303

+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+

PCR

ImmunoStriptest
for%purity
nptII Mon531 Cry1Ab/Cry1Ac

ELISA

+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+

(g/gorppm)
0.49
0.45
0.34
0.47
0.61
1.19
0.44
0.38
1.93
0.57
0.23
0.40
0.67
0.53
1.09
0.28
0.70
0.25
0.10
0.18
0.82
0.26
0.50
1.35
0.55
0.49
0.61
0.98
0.33
0.78
0.18
0.71
0.42
0.41
0.62
0.60
0.60
0.47
0.32
0.31
0.60
0.57

+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+

80
100
60
80
60
60
80
80
80
80
60
80
60
80
80
80
60
60
60
80
80
80
60
60
60
40
60
80
80
80
80
60
60
80
60
80
60
60
80
60
60
60

Cry1Ac

Sr. Cotton
No Lines
(Code)
43.
44.
45.
46.
47.
48.
49.
50.
51.
52.
53.
54.
55.
56.
57.
58.
59.
60.
61.
62.
63.
64.
65.
66.
67.
68.
69.
70.
71.
72.

V43
V44
V45
V46
V47
V48
V49
V50
V51
V52
V53
V54
V55
V56
V57
V58
V59
V60
V61
V62
V63
V64
V65
V66
V67
V68
V69
V70
V71
V72

73.

V73

74.

V74

75.

V75

Variety

PCR

Sad1
BZU75
VH282
CRIS508
MM58
IRNIBGE5
SLH4
IUB222
IRNIBGE4
CIM600
BH180
CIM602
CEMB55
CIM599
CEMB44
BtBH178
GS444
DNH105
CIM612
PB38
IUB11
MPSII
NIAB112
Cyto124
JS212
VH300
CRIS510
NIA80
CIM591
BH176
CIM573(Non
BtStandard)
CRIS342(Non
BtStandard)
CIM598
(Btstandard)
MNH886
(BtStandard)

ImmunoStriptest
for%purity
nptII Mon531 Cry1Ab/Cry1Ac

Cry1Ac

80
60
80
80
60
80
60
80
80
60
80
80
80
80
60
40
40
60
80
40
0
0
0
60
0
0
60
0
0
0

(g/gorppm)
0.10
0.23
0.43
0.44
0.15
0.83
0.19
0.16
0.36
0.31
0.40
0.84
0.12
0.19
0.43
0.23
0.23
0.24
0.26
0.22
0.00
0.00
0.00
0.40
0.00
0.00
0.19
0.00
0.00
0.00

80

0.12

80

0.21

60

0.40

+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+

+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+

+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+

ELISA